|Institutional Source||Beutler Lab|
|Gene Name||CD14 antigen|
|Synonyms||monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein|
|Is this an essential gene?||Probably non essential (E-score: 0.121)|
|Stock #||R6006 (G1)|
|Chromosomal Location||36725074-36726736 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 36726282 bp|
|Amino Acid Change||Aspartic acid to Glycine at position 40 (D40G)|
|Ref Sequence||ENSEMBL: ENSMUSP00000056669 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000061829]|
|PDB Structure||Crystal structure of CD14 [X-RAY DIFFRACTION]|
|Predicted Effect||possibly damaging
AA Change: D40G
PolyPhen 2 Score 0.506 (Sensitivity: 0.88; Specificity: 0.90)
AA Change: D40G
|Coding Region Coverage||
FUNCTION: This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]
PHENOTYPE: Homozygous null mice exhibit macrophages with impaired responses to LPS or E.coli, resulting in a reduction or loss of cytokine production. Macrophages also cannot contain vesicular stomatitis virus infection. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cd14||
(F):5'- GCAGGGCTCCGAATAGAATC -3'
(R):5'- AGACAGTCTTGGGGTGGAACTG -3'
(F):5'- GCTCCGAATAGAATCCGACTAGG -3'
(R):5'- TGGAGCCAACCTGAGGAATCTC -3'