Mutagenetix > Incidental Mutation

Incidental Mutation 'R0633:Wrap73'

58056

Institutional Source

Beutler Lab

Gene Symbol

Wrap73

Ensembl Gene

ENSMUSG00000029029

Gene Name

WD repeat containing, antisense to Trp73

Synonyms

DD57, Wdr8, 5330425N03Rik, 2610044M17Rik

MMRRC Submission

038822-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.652) question?

Stock #

R0633 (G1)

Quality Score

156

Status

Not validated

Chromosome

Chromosomal Location

154142372-154167420 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 154142491 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Tyrosine at position 16 (F16Y)

Ref Sequence

ENSEMBL: ENSMUSP00000030895 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030895] [ENSMUST00000105644]

AlphaFold

Q9JM98

Predicted Effect

probably damaging
Transcript: ENSMUST00000030895
AA Change: F16Y

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000030895
Gene: ENSMUSG00000029029
AA Change: F16Y

Domain	Start	End	E-Value	Type
Blast:WD40	38	77	4e-18	BLAST
Blast:WD40	81	120	6e-16	BLAST
Blast:WD40	125	163	9e-6	BLAST
WD40	167	208	2.28e2	SMART
WD40	215	251	1.58e-2	SMART
WD40	319	360	2.29e1	SMART
WD40	363	401	4.18e-2	SMART
Blast:WD40	402	443	2e-19	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000105644

SMART Domains

Protein: ENSMUSP00000101269
Gene: ENSMUSG00000029026

Domain	Start	End	E-Value	Type
Pfam:P53	112	308	3.1e-115	PFAM
Pfam:P53_tetramer	344	383	8.3e-21	PFAM
low complexity region	390	398	N/A	INTRINSIC
SAM	486	552	2.71e-5	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142665

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.4%
20x: 94.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Studies of the related mouse protein suggest that the encoded protein may play a role in the process of ossification. [provided by RefSeq, Mar 2009]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810474O19Rik	A	T	6: 149,325,701 (GRCm38)	I82L	probably benign	Het
4921530L21Rik	T	G	14: 95,881,943 (GRCm38)	N45K	probably damaging	Het
4933408B17Rik	A	G	18: 34,586,266 (GRCm38)	V167A	possibly damaging	Het
Adamts8	A	T	9: 30,943,511 (GRCm38)	R18S	probably damaging	Het
Adgb	G	A	10: 10,391,729 (GRCm38)	A923V	probably benign	Het
Aldh1a3	A	G	7: 66,400,222 (GRCm38)	V416A	probably damaging	Het
Alox5	C	T	6: 116,420,384 (GRCm38)	G280R	probably damaging	Het
Anapc5	A	T	5: 122,800,632 (GRCm38)	Y360N	probably damaging	Het
Apbb1	C	T	7: 105,558,963 (GRCm38)	V685I	probably damaging	Het
Apc2	C	A	10: 80,307,455 (GRCm38)	A463E	probably damaging	Het
Arhgap21	C	T	2: 20,855,387 (GRCm38)	W1170*	probably null	Het
Atat1	G	A	17: 35,901,423 (GRCm38)	R305C	probably damaging	Het
Cars2	T	C	8: 11,550,511 (GRCm38)	D56G	probably benign	Het
Cdc42bpb	T	C	12: 111,345,555 (GRCm38)	I108V	probably damaging	Het
Cftr	T	A	6: 18,305,980 (GRCm38)	I1255K	probably damaging	Het
Ckap5	T	C	2: 91,550,743 (GRCm38)	L148P	probably damaging	Het
Cntn4	A	G	6: 106,679,248 (GRCm38)		probably null	Het
Cpe	G	A	8: 64,609,203 (GRCm38)	P273L	probably damaging	Het
Cpsf7	A	G	19: 10,531,782 (GRCm38)	D19G	probably benign	Het
Ddx25	C	A	9: 35,545,972 (GRCm38)	R349L	probably damaging	Het
Depdc7	T	C	2: 104,722,881 (GRCm38)	D446G	probably benign	Het
Det1	T	A	7: 78,843,935 (GRCm38)	N107I	probably benign	Het
Dock6	A	T	9: 21,844,417 (GRCm38)	D170E	probably benign	Het
Dvl1	C	T	4: 155,858,295 (GRCm38)	L673F	probably damaging	Het
Gucy1b1	A	T	3: 82,045,460 (GRCm38)	I222K	probably benign	Het
Hfm1	T	C	5: 106,917,601 (GRCm38)	T71A	possibly damaging	Het
Ikzf1	A	G	11: 11,769,223 (GRCm38)	E310G	probably damaging	Het
Impg1	T	C	9: 80,394,155 (GRCm38)	E163G	possibly damaging	Het
Itpr2	G	T	6: 146,374,456 (GRCm38)	H426Q	probably damaging	Het
Itpripl2	C	T	7: 118,490,256 (GRCm38)	G360D	probably benign	Het
Kif14	C	T	1: 136,527,305 (GRCm38)	R1572C	probably damaging	Het
L3mbtl3	A	T	10: 26,302,685 (GRCm38)	H568Q	unknown	Het
Lgi2	A	G	5: 52,554,460 (GRCm38)	Y173H	probably damaging	Het
Lpar5	A	C	6: 125,081,991 (GRCm38)	Y225S	probably benign	Het
Lpin3	A	G	2: 160,903,974 (GRCm38)	H675R	probably damaging	Het
Lrp2	C	A	2: 69,448,120 (GRCm38)	G3963V	probably damaging	Het
Man1a2	G	T	3: 100,684,575 (GRCm38)	D13E	possibly damaging	Het
Map1a	T	C	2: 121,308,014 (GRCm38)	V2753A	probably damaging	Het
Mitf	C	A	6: 98,003,904 (GRCm38)	N97K	probably damaging	Het
Msh2	A	G	17: 87,672,810 (GRCm38)		probably null	Het
Msr1	T	C	8: 39,620,000 (GRCm38)	E170G	probably damaging	Het
Myrip	C	A	9: 120,388,236 (GRCm38)	R79S	probably damaging	Het
Nek10	G	A	14: 14,857,782 (GRCm38)		probably null	Het
Neto1	C	T	18: 86,404,729 (GRCm38)	R104*	probably null	Het
Nom1	A	C	5: 29,451,100 (GRCm38)	K821T	probably damaging	Het
Nrxn1	A	G	17: 90,704,181 (GRCm38)	V340A	probably damaging	Het
Nxpe4	A	T	9: 48,396,597 (GRCm38)	I334F	probably benign	Het
Olfr1043	T	A	2: 86,162,091 (GRCm38)	N286I	probably damaging	Het
Olfr1065	C	T	2: 86,445,129 (GRCm38)	M284I	probably benign	Het
Olfr1247	T	C	2: 89,609,374 (GRCm38)	M243V	probably benign	Het
Olfr1489	T	C	19: 13,633,336 (GRCm38)	V75A	probably damaging	Het
Olfr382	A	G	11: 73,516,927 (GRCm38)	S91P	probably benign	Het
Olfr705	T	C	7: 106,713,977 (GRCm38)	K235E	probably benign	Het
Padi4	A	G	4: 140,757,585 (GRCm38)	S322P	probably damaging	Het
Peli3	A	G	19: 4,941,782 (GRCm38)	Y44H	probably damaging	Het
Prdm4	A	G	10: 85,907,903 (GRCm38)	S163P	probably damaging	Het
Prom2	T	C	2: 127,539,525 (GRCm38)	D227G	probably benign	Het
Ptgfr	C	T	3: 151,801,763 (GRCm38)	R321H	probably benign	Het
Rgs3	G	A	4: 62,625,906 (GRCm38)	R136H	probably damaging	Het
Rgsl1	T	G	1: 153,844,107 (GRCm38)	N3T	possibly damaging	Het
Rif1	T	C	2: 52,112,563 (GRCm38)	S2010P	probably benign	Het
Rngtt	T	C	4: 33,368,690 (GRCm38)	F408L	probably damaging	Het
Rtn3	T	G	19: 7,457,593 (GRCm38)	T326P	probably benign	Het
Slc18b1	A	C	10: 23,806,038 (GRCm38)	M167L	probably benign	Het
Slc22a26	A	G	19: 7,788,210 (GRCm38)		probably null	Het
Slitrk6	T	C	14: 110,751,885 (GRCm38)	D130G	probably damaging	Het
Snap47	A	G	11: 59,428,613 (GRCm38)	V233A	probably benign	Het
Sumf1	A	C	6: 108,144,671 (GRCm38)	Y158D	probably damaging	Het
Tbc1d15	A	T	10: 115,220,310 (GRCm38)	H252Q	probably benign	Het
Thsd7b	T	C	1: 130,188,526 (GRCm38)	S1339P	possibly damaging	Het
Tmem45a2	T	C	16: 57,049,414 (GRCm38)	I56V	probably benign	Het
Ttc21b	A	G	2: 66,236,233 (GRCm38)	S359P	probably benign	Het
Ttc27	T	C	17: 74,729,977 (GRCm38)	I215T	probably benign	Het
Ttn	C	T	2: 76,724,195 (GRCm38)	V30759I	possibly damaging	Het
Vdac3	T	C	8: 22,580,388 (GRCm38)	N168S	probably damaging	Het
Wdr7	T	C	18: 63,865,300 (GRCm38)	V1106A	probably benign	Het
Zfat	C	A	15: 68,180,803 (GRCm38)	D381Y	probably damaging	Het

Other mutations in Wrap73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00793:Wrap73	APN	4	154,152,639 (GRCm38)	missense	probably damaging	0.99
IGL01562:Wrap73	APN	4	154,145,337 (GRCm38)	missense	possibly damaging	0.63
IGL01863:Wrap73	APN	4	154,145,333 (GRCm38)	missense	probably benign	0.02
IGL02342:Wrap73	APN	4	154,148,780 (GRCm38)	missense	probably benign	0.36
IGL03012:Wrap73	APN	4	154,145,234 (GRCm38)	splice site	probably benign
IGL03303:Wrap73	APN	4	154,146,543 (GRCm38)	missense	probably damaging	0.98
R0128:Wrap73	UTSW	4	154,142,500 (GRCm38)	missense	possibly damaging	0.81
R0455:Wrap73	UTSW	4	154,148,743 (GRCm38)	missense	possibly damaging	0.63
R0524:Wrap73	UTSW	4	154,145,307 (GRCm38)	missense	probably damaging	1.00
R0528:Wrap73	UTSW	4	154,145,319 (GRCm38)	missense	probably damaging	1.00
R0533:Wrap73	UTSW	4	154,156,154 (GRCm38)	missense	possibly damaging	0.91
R0533:Wrap73	UTSW	4	154,151,649 (GRCm38)	missense	probably damaging	1.00
R1118:Wrap73	UTSW	4	154,152,427 (GRCm38)	splice site	probably null
R1669:Wrap73	UTSW	4	154,156,131 (GRCm38)	missense	probably damaging	0.99
R1725:Wrap73	UTSW	4	154,148,752 (GRCm38)	missense	possibly damaging	0.73
R2070:Wrap73	UTSW	4	154,148,743 (GRCm38)	missense	possibly damaging	0.63
R4530:Wrap73	UTSW	4	154,156,707 (GRCm38)	unclassified	probably benign
R4669:Wrap73	UTSW	4	154,151,696 (GRCm38)	missense	probably benign	0.26
R4969:Wrap73	UTSW	4	154,152,681 (GRCm38)	missense	probably damaging	1.00
R5254:Wrap73	UTSW	4	154,155,346 (GRCm38)	missense	probably benign	0.00
R5334:Wrap73	UTSW	4	154,145,274 (GRCm38)	missense	probably damaging	0.97
R5428:Wrap73	UTSW	4	154,145,274 (GRCm38)	missense	probably damaging	0.97
R5431:Wrap73	UTSW	4	154,145,274 (GRCm38)	missense	probably damaging	0.97
R5728:Wrap73	UTSW	4	154,154,642 (GRCm38)	critical splice donor site	probably null
R7338:Wrap73	UTSW	4	154,152,586 (GRCm38)	missense	probably benign	0.26
R7426:Wrap73	UTSW	4	154,156,127 (GRCm38)	missense	probably damaging	1.00
R7480:Wrap73	UTSW	4	154,152,586 (GRCm38)	missense	probably benign	0.26
R7680:Wrap73	UTSW	4	154,156,622 (GRCm38)	missense	probably benign	0.20

Predicted Primers

PCR Primer
(F):5'- GCCCCTTTAAGACTCAAACTCGGAC -3'
(R):5'- GTGCCCTCAAATGTGTGAAAGCAG -3'

Sequencing Primer
(F):5'- TTTAAGACTCAAACTCGGACTTCCC -3'
(R):5'- GTGTGAAAGCAGAGTCATCTTATCC -3'

Posted On

2013-07-11