Mutagenetix > Incidental Mutation

Incidental Mutation 'R8242:Vmn2r13'

637600

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r13

Ensembl Gene

ENSMUSG00000091635

Gene Name

vomeronasal 2, receptor 13

Synonyms

Gm4867

MMRRC Submission

067647-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R8242 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109303889-109339973 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 109322872 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 139 (Y139C)

Ref Sequence

ENSEMBL: ENSMUSP00000052977 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053253]

AlphaFold

L7N1X2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000053253
AA Change: Y139C

PolyPhen 2 Score 0.652 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000052977
Gene: ENSMUSG00000091635
AA Change: Y139C

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	76	463	2.8e-29	PFAM
Pfam:NCD3G	506	560	1.3e-18	PFAM
Pfam:7tm_3	593	828	1.8e-54	PFAM

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.2%
20x: 98.2%

Validation Efficiency

98% (42/43)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy10	T	C	1: 165,374,118 (GRCm39)	Y736H	possibly damaging	Het
Akr1c12	G	A	13: 4,322,269 (GRCm39)	R258*	probably null	Het
Ankfn1	C	G	11: 89,417,271 (GRCm39)		probably null	Het
Arfgef3	T	C	10: 18,505,824 (GRCm39)	K947E	probably benign	Het
Barx2	C	A	9: 31,824,227 (GRCm39)	R54L	probably damaging	Het
BC030500	T	C	8: 59,365,388 (GRCm39)	I13T	unknown	Het
Bcl11a	T	A	11: 24,113,208 (GRCm39)	F184I	probably benign	Het
Cfap251	T	A	5: 123,411,914 (GRCm39)	D515E	possibly damaging	Het
Cfap43	A	G	19: 47,885,808 (GRCm39)	L269P	probably damaging	Het
Col8a1	G	T	16: 57,452,721 (GRCm39)	P94Q	possibly damaging	Het
Coq5	T	C	5: 115,417,806 (GRCm39)	V12A	probably benign	Het
Csmd1	A	G	8: 16,760,670 (GRCm39)	V155A	probably benign	Het
Defb22	A	C	2: 152,328,007 (GRCm39)	C59W	probably damaging	Het
Dsg3	A	G	18: 20,669,980 (GRCm39)	T665A	possibly damaging	Het
Gm10542	A	G	18: 44,337,708 (GRCm39)	D62G	possibly damaging	Het
Gm45861	G	A	8: 28,038,821 (GRCm39)	S987N	unknown	Het
Gm5591	T	A	7: 38,219,746 (GRCm39)	T376S	probably benign	Het
Hps3	C	T	3: 20,068,290 (GRCm39)	R588H	possibly damaging	Het
Itpr1	A	T	6: 108,363,658 (GRCm39)	D760V	probably benign	Het
Mfsd8	G	A	3: 40,789,628 (GRCm39)	R140C	probably damaging	Het
Mroh2b	A	T	15: 4,938,522 (GRCm39)	R270S	probably benign	Het
Mroh4	G	T	15: 74,488,157 (GRCm39)	H350N	possibly damaging	Het
Nsd3	G	T	8: 26,196,567 (GRCm39)	E1183*	probably null	Het
Or13a19	T	A	7: 139,902,696 (GRCm39)	L28*	probably null	Het
Or4p7	A	G	2: 88,222,418 (GRCm39)	I276V	possibly damaging	Het
Or51f23b	T	C	7: 102,402,306 (GRCm39)	M277V	probably benign	Het
Or8k53	A	T	2: 86,177,426 (GRCm39)	M228K	probably damaging	Het
Pex5l	T	A	3: 33,060,184 (GRCm39)	T196S	probably benign	Het
Phlpp2	T	C	8: 110,666,834 (GRCm39)	V1121A	probably benign	Het
Rab3gap2	T	A	1: 184,954,050 (GRCm39)	S41T	probably benign	Het
Rad51c	A	T	11: 87,280,712 (GRCm39)	N293K	probably damaging	Het
Rgs3	C	A	4: 62,538,022 (GRCm39)	Q18K	probably benign	Het
Ripk2	C	T	4: 16,124,430 (GRCm39)	G426D	probably benign	Het
Rpl22l1	T	C	3: 28,860,914 (GRCm39)	V44A	possibly damaging	Het
Sntb1	C	T	15: 55,655,629 (GRCm39)	A196T	possibly damaging	Het
Sorcs3	C	T	19: 48,194,913 (GRCm39)	R99W	possibly damaging	Het
Tdrd6	T	C	17: 43,939,821 (GRCm39)	E409G	probably damaging	Het
Timm50	A	T	7: 28,007,836 (GRCm39)	D105E	probably benign	Het
Tsga10	A	T	1: 37,846,182 (GRCm39)	S315T	probably benign	Het
Ttpa	G	A	4: 20,028,511 (GRCm39)	C256Y	probably damaging	Het
Vinac1	G	T	2: 128,881,313 (GRCm39)	C204*	probably null	Het
Washc5	A	G	15: 59,215,971 (GRCm39)	S803P	probably damaging	Het
Zfp985	A	C	4: 147,668,639 (GRCm39)	K502N	possibly damaging	Het

Other mutations in Vmn2r13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00965:Vmn2r13	APN	5	109,303,964 (GRCm39)	missense	probably damaging	1.00
IGL01373:Vmn2r13	APN	5	109,304,568 (GRCm39)	missense	probably damaging	1.00
IGL01946:Vmn2r13	APN	5	109,322,085 (GRCm39)	missense	probably benign	0.01
IGL01971:Vmn2r13	APN	5	109,321,981 (GRCm39)	missense	probably benign	0.01
IGL02636:Vmn2r13	APN	5	109,339,883 (GRCm39)	missense	probably damaging	0.98
IGL03062:Vmn2r13	APN	5	109,304,148 (GRCm39)	missense	probably damaging	1.00
IGL03173:Vmn2r13	APN	5	109,319,645 (GRCm39)	missense	possibly damaging	0.95
IGL03301:Vmn2r13	APN	5	109,305,955 (GRCm39)	missense	probably damaging	0.99
IGL03383:Vmn2r13	APN	5	109,304,398 (GRCm39)	missense	probably damaging	0.98
IGL03048:Vmn2r13	UTSW	5	109,304,151 (GRCm39)	missense	probably damaging	1.00
R0123:Vmn2r13	UTSW	5	109,322,915 (GRCm39)	missense	probably benign	0.00
R0134:Vmn2r13	UTSW	5	109,322,915 (GRCm39)	missense	probably benign	0.00
R0220:Vmn2r13	UTSW	5	109,304,332 (GRCm39)	missense	probably damaging	1.00
R0225:Vmn2r13	UTSW	5	109,322,915 (GRCm39)	missense	probably benign	0.00
R0393:Vmn2r13	UTSW	5	109,304,395 (GRCm39)	missense	probably benign	0.01
R0410:Vmn2r13	UTSW	5	109,321,679 (GRCm39)	missense	probably benign	0.35
R0787:Vmn2r13	UTSW	5	109,304,713 (GRCm39)	missense	probably damaging	0.99
R1200:Vmn2r13	UTSW	5	109,322,068 (GRCm39)	missense	probably damaging	1.00
R1448:Vmn2r13	UTSW	5	109,322,001 (GRCm39)	missense	probably damaging	1.00
R1782:Vmn2r13	UTSW	5	109,306,040 (GRCm39)	missense	probably benign	0.08
R1939:Vmn2r13	UTSW	5	109,339,852 (GRCm39)	missense	possibly damaging	0.88
R2029:Vmn2r13	UTSW	5	109,339,943 (GRCm39)	missense	probably benign	0.13
R2125:Vmn2r13	UTSW	5	109,306,058 (GRCm39)	missense	probably benign	0.00
R2126:Vmn2r13	UTSW	5	109,306,058 (GRCm39)	missense	probably benign	0.00
R2379:Vmn2r13	UTSW	5	109,319,644 (GRCm39)	missense	probably benign	0.05
R2680:Vmn2r13	UTSW	5	109,322,178 (GRCm39)	missense	possibly damaging	0.66
R2888:Vmn2r13	UTSW	5	109,339,840 (GRCm39)	missense	possibly damaging	0.88
R2889:Vmn2r13	UTSW	5	109,339,840 (GRCm39)	missense	possibly damaging	0.88
R2890:Vmn2r13	UTSW	5	109,339,840 (GRCm39)	missense	possibly damaging	0.88
R3014:Vmn2r13	UTSW	5	109,319,627 (GRCm39)	missense	possibly damaging	0.81
R3683:Vmn2r13	UTSW	5	109,304,721 (GRCm39)	missense	probably damaging	1.00
R4074:Vmn2r13	UTSW	5	109,304,566 (GRCm39)	missense	probably damaging	1.00
R4599:Vmn2r13	UTSW	5	109,304,322 (GRCm39)	missense	probably damaging	1.00
R4614:Vmn2r13	UTSW	5	109,323,065 (GRCm39)	missense	probably benign	0.01
R4805:Vmn2r13	UTSW	5	109,304,331 (GRCm39)	missense	probably damaging	1.00
R4822:Vmn2r13	UTSW	5	109,321,938 (GRCm39)	missense	probably damaging	0.99
R4943:Vmn2r13	UTSW	5	109,322,915 (GRCm39)	missense	probably benign	0.00
R5263:Vmn2r13	UTSW	5	109,321,841 (GRCm39)	missense	probably benign	0.00
R5297:Vmn2r13	UTSW	5	109,339,805 (GRCm39)	missense	probably benign	0.00
R5502:Vmn2r13	UTSW	5	109,321,580 (GRCm39)	missense	probably damaging	1.00
R5554:Vmn2r13	UTSW	5	109,339,860 (GRCm39)	missense	possibly damaging	0.49
R5563:Vmn2r13	UTSW	5	109,321,846 (GRCm39)	missense	probably benign	0.00
R5819:Vmn2r13	UTSW	5	109,321,966 (GRCm39)	missense	possibly damaging	0.79
R6074:Vmn2r13	UTSW	5	109,322,167 (GRCm39)	missense	probably benign	0.04
R6416:Vmn2r13	UTSW	5	109,321,982 (GRCm39)	missense	probably damaging	0.99
R6419:Vmn2r13	UTSW	5	109,323,085 (GRCm39)	missense	possibly damaging	0.87
R6484:Vmn2r13	UTSW	5	109,304,540 (GRCm39)	nonsense	probably null
R6486:Vmn2r13	UTSW	5	109,304,425 (GRCm39)	missense	probably benign	0.05
R6545:Vmn2r13	UTSW	5	109,304,806 (GRCm39)	splice site	probably null
R6700:Vmn2r13	UTSW	5	109,322,938 (GRCm39)	missense	probably benign	0.00
R6897:Vmn2r13	UTSW	5	109,306,015 (GRCm39)	missense	possibly damaging	0.90
R6957:Vmn2r13	UTSW	5	109,304,753 (GRCm39)	nonsense	probably null
R7276:Vmn2r13	UTSW	5	109,321,645 (GRCm39)	missense	probably damaging	1.00
R7363:Vmn2r13	UTSW	5	109,339,909 (GRCm39)	missense	probably benign	0.03
R7443:Vmn2r13	UTSW	5	109,339,909 (GRCm39)	missense	probably benign	0.03
R7555:Vmn2r13	UTSW	5	109,319,557 (GRCm39)	splice site	probably null
R7607:Vmn2r13	UTSW	5	109,321,506 (GRCm39)	missense	probably damaging	0.98
R7719:Vmn2r13	UTSW	5	109,319,618 (GRCm39)	missense	probably benign	0.00
R8116:Vmn2r13	UTSW	5	109,322,926 (GRCm39)	missense	probably benign	0.12
R8294:Vmn2r13	UTSW	5	109,322,978 (GRCm39)	missense	probably benign	0.02
R8340:Vmn2r13	UTSW	5	109,322,006 (GRCm39)	missense	probably benign	0.00
R8692:Vmn2r13	UTSW	5	109,319,514 (GRCm39)	missense	probably benign	0.03
R8742:Vmn2r13	UTSW	5	109,304,263 (GRCm39)	missense	probably benign	0.02
R9022:Vmn2r13	UTSW	5	109,304,242 (GRCm39)	missense	possibly damaging	0.94
R9281:Vmn2r13	UTSW	5	109,303,953 (GRCm39)	missense	probably damaging	1.00
R9529:Vmn2r13	UTSW	5	109,304,064 (GRCm39)	missense	probably damaging	1.00
R9708:Vmn2r13	UTSW	5	109,322,007 (GRCm39)	missense	probably benign	0.00
R9746:Vmn2r13	UTSW	5	109,339,773 (GRCm39)	critical splice donor site	probably null
X0066:Vmn2r13	UTSW	5	109,304,085 (GRCm39)	missense	probably benign	0.44

Predicted Primers

PCR Primer
(F):5'- TTCTTGTAGGCACATGTAAATGGG -3'
(R):5'- TGCCTTGTTTTAGAATACCAGCAAG -3'

Sequencing Primer
(F):5'- TGACTCATCACTACCTGTAAGGGG -3'
(R):5'- CCAGCAAGAAGATATGAGTTTTTCC -3'

Posted On

2020-07-13