Mutagenetix > Incidental Mutation

Incidental Mutation 'R8554:Vmn2r65'

660024

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r65

Ensembl Gene

ENSMUSG00000066372

Gene Name

vomeronasal 2, receptor 65

Synonyms

ENSMUSG00000070600

MMRRC Submission

068517-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R8554 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

84589377-84613217 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 84595960 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 241 (I241M)

Ref Sequence

ENSEMBL: ENSMUSP00000036551 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044583]

AlphaFold

G3X931

Predicted Effect

probably benign
Transcript: ENSMUST00000044583
AA Change: I241M

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000036551
Gene: ENSMUSG00000066372
AA Change: I241M

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	73	435	5.6e-41	PFAM
Pfam:NCD3G	501	553	1.3e-21	PFAM
Pfam:7tm_3	584	821	2.3e-53	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adtrp	G	A	13: 41,969,636 (GRCm39)	T89I	possibly damaging	Het
Aopep	T	A	13: 63,444,711 (GRCm39)	Y94N	possibly damaging	Het
Apc	T	C	18: 34,445,999 (GRCm39)	V947A	probably damaging	Het
Apob	A	C	12: 8,037,830 (GRCm39)	K334N	probably damaging	Het
Camk2d	A	T	3: 126,564,448 (GRCm39)	Q119L	possibly damaging	Het
Ccdc60	A	T	5: 116,328,171 (GRCm39)	F98I	probably damaging	Het
Cd47	A	G	16: 49,688,304 (GRCm39)	T22A	probably benign	Het
Crat	A	G	2: 30,300,035 (GRCm39)	V115A	probably benign	Het
Csmd3	T	C	15: 47,507,538 (GRCm39)	M2992V	probably benign	Het
Dsg4	T	A	18: 20,586,100 (GRCm39)	N263K	probably damaging	Het
Eln	A	C	5: 134,738,964 (GRCm39)		probably benign	Het
Ep300	A	G	15: 81,523,228 (GRCm39)	E1284G	unknown	Het
Fam193a	T	A	5: 34,633,115 (GRCm39)	M122K	probably benign	Het
Fcgr1	A	T	3: 96,199,788 (GRCm39)	W40R	probably damaging	Het
Gm3486	T	A	14: 41,209,119 (GRCm39)	Q84L	probably damaging	Het
Golga2	G	A	2: 32,183,357 (GRCm39)	D80N	probably damaging	Het
Isg20	T	C	7: 78,566,425 (GRCm39)	Y125H	probably benign	Het
Kdf1	A	G	4: 133,256,188 (GRCm39)	I302V	probably damaging	Het
Kics2	T	A	10: 121,575,960 (GRCm39)	I27N	probably benign	Het
Krt33a	T	A	11: 99,903,209 (GRCm39)	T278S	possibly damaging	Het
Lrp1b	T	A	2: 41,234,495 (GRCm39)	Q1038L	probably benign	Het
Marchf6	G	A	15: 31,482,976 (GRCm39)	H476Y	probably damaging	Het
Myom1	A	G	17: 71,343,448 (GRCm39)	E215G	possibly damaging	Het
Or2aa1	A	T	11: 59,480,312 (GRCm39)	L201Q	possibly damaging	Het
Or2h15	T	A	17: 38,441,489 (GRCm39)	Q198L	probably damaging	Het
Or4c122	T	C	2: 89,079,595 (GRCm39)	T148A	possibly damaging	Het
Pate11	A	G	9: 36,387,788 (GRCm39)	I25V	probably benign	Het
Pdlim2	T	A	14: 70,408,698 (GRCm39)	T173S	probably benign	Het
Pitpnb	T	C	5: 111,494,372 (GRCm39)	M74T	probably benign	Het
Pou2f2	C	A	7: 24,814,981 (GRCm39)		probably benign	Het
Rab35	A	C	5: 115,783,690 (GRCm39)		probably null	Het
Rasef	A	T	4: 73,645,844 (GRCm39)	D508E	probably benign	Het
Rev3l	T	C	10: 39,682,838 (GRCm39)	S319P	probably benign	Het
Rftn1	G	T	17: 50,354,408 (GRCm39)	A318D	probably damaging	Het
Rgl3	A	G	9: 21,900,159 (GRCm39)	S44P	probably benign	Het
Rnf214	C	A	9: 45,778,797 (GRCm39)		probably null	Het
Rpsa	T	C	9: 119,958,317 (GRCm39)	V76A	possibly damaging	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,130 (GRCm39)		probably benign	Het
Senp7	T	C	16: 55,978,973 (GRCm39)	V529A	probably benign	Het
Siglecg	C	T	7: 43,058,320 (GRCm39)	S69L	probably benign	Het
Sos1	G	T	17: 80,705,842 (GRCm39)	T1243K	probably damaging	Het
Tent5a	T	C	9: 85,208,784 (GRCm39)	D13G	possibly damaging	Het
Tlcd3a	A	G	11: 76,096,244 (GRCm39)	H124R	probably damaging	Het
Tmc2	A	G	2: 130,106,084 (GRCm39)	T872A	probably benign	Het
Tnfrsf8	A	C	4: 145,023,511 (GRCm39)	C107W	probably damaging	Het
Tnn	T	C	1: 159,937,986 (GRCm39)	Y913C	probably damaging	Het
Ttn	T	C	2: 76,568,376 (GRCm39)	T19179A	probably damaging	Het
Usp42	T	A	5: 143,706,137 (GRCm39)	K294N	probably damaging	Het
Vmn1r123	T	A	7: 20,896,971 (GRCm39)	C288S	probably benign	Het
Vmn1r29	T	A	6: 58,285,191 (GRCm39)	*304K	probably null	Het
Vmn2r1	T	C	3: 63,997,334 (GRCm39)	I330T	probably damaging	Het
Vmn2r16	A	G	5: 109,511,997 (GRCm39)	I735V	probably benign	Het

Other mutations in Vmn2r65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00942:Vmn2r65	APN	7	84,592,761 (GRCm39)	missense	probably damaging	0.99
IGL01104:Vmn2r65	APN	7	84,589,996 (GRCm39)	missense	possibly damaging	0.93
IGL01154:Vmn2r65	APN	7	84,592,729 (GRCm39)	missense	probably benign	0.00
IGL01453:Vmn2r65	APN	7	84,589,708 (GRCm39)	missense	probably damaging	1.00
IGL01675:Vmn2r65	APN	7	84,596,587 (GRCm39)	missense	probably benign	0.00
IGL01748:Vmn2r65	APN	7	84,589,507 (GRCm39)	missense	probably damaging	1.00
IGL01997:Vmn2r65	APN	7	84,589,978 (GRCm39)	missense	probably damaging	1.00
IGL02527:Vmn2r65	APN	7	84,595,724 (GRCm39)	missense	possibly damaging	0.82
IGL02617:Vmn2r65	APN	7	84,589,549 (GRCm39)	missense	probably damaging	1.00
IGL02676:Vmn2r65	APN	7	84,589,381 (GRCm39)	missense	possibly damaging	0.86
IGL03084:Vmn2r65	APN	7	84,592,354 (GRCm39)	missense	probably damaging	1.00
A4554:Vmn2r65	UTSW	7	84,595,791 (GRCm39)	missense	probably damaging	0.96
PIT4651001:Vmn2r65	UTSW	7	84,595,461 (GRCm39)	missense	probably benign	0.12
R0322:Vmn2r65	UTSW	7	84,595,756 (GRCm39)	missense	probably benign	0.01
R0453:Vmn2r65	UTSW	7	84,595,442 (GRCm39)	missense	probably benign	0.03
R0862:Vmn2r65	UTSW	7	84,592,853 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1508:Vmn2r65	UTSW	7	84,589,886 (GRCm39)	missense	probably benign	0.00
R1687:Vmn2r65	UTSW	7	84,590,026 (GRCm39)	missense	probably benign	0.02
R1876:Vmn2r65	UTSW	7	84,595,505 (GRCm39)	missense	probably damaging	1.00
R2136:Vmn2r65	UTSW	7	84,592,781 (GRCm39)	missense	probably damaging	0.96
R2259:Vmn2r65	UTSW	7	84,590,119 (GRCm39)	missense	possibly damaging	0.86
R2338:Vmn2r65	UTSW	7	84,590,051 (GRCm39)	missense	possibly damaging	0.86
R2880:Vmn2r65	UTSW	7	84,613,094 (GRCm39)	missense	probably damaging	1.00
R3410:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3411:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3770:Vmn2r65	UTSW	7	84,589,623 (GRCm39)	missense	probably damaging	1.00
R3800:Vmn2r65	UTSW	7	84,589,738 (GRCm39)	missense	possibly damaging	0.93
R3850:Vmn2r65	UTSW	7	84,595,859 (GRCm39)	missense	probably benign	0.00
R4105:Vmn2r65	UTSW	7	84,595,691 (GRCm39)	missense	probably benign	0.03
R4568:Vmn2r65	UTSW	7	84,596,677 (GRCm39)	missense	probably damaging	1.00
R4677:Vmn2r65	UTSW	7	84,613,082 (GRCm39)	missense	possibly damaging	0.55
R4768:Vmn2r65	UTSW	7	84,596,602 (GRCm39)	missense	probably damaging	1.00
R4778:Vmn2r65	UTSW	7	84,592,801 (GRCm39)	missense	possibly damaging	0.47
R5057:Vmn2r65	UTSW	7	84,589,819 (GRCm39)	missense	probably damaging	1.00
R5279:Vmn2r65	UTSW	7	84,589,849 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r65	UTSW	7	84,595,542 (GRCm39)	missense	probably damaging	0.99
R5394:Vmn2r65	UTSW	7	84,595,862 (GRCm39)	missense	probably benign	0.04
R5487:Vmn2r65	UTSW	7	84,595,529 (GRCm39)	missense	possibly damaging	0.76
R5580:Vmn2r65	UTSW	7	84,596,802 (GRCm39)	missense	probably damaging	0.99
R5638:Vmn2r65	UTSW	7	84,590,047 (GRCm39)	missense	probably damaging	1.00
R5673:Vmn2r65	UTSW	7	84,596,615 (GRCm39)	missense	probably benign	0.23
R5688:Vmn2r65	UTSW	7	84,589,900 (GRCm39)	missense	probably benign	0.00
R5935:Vmn2r65	UTSW	7	84,592,869 (GRCm39)	missense	probably benign	0.00
R6354:Vmn2r65	UTSW	7	84,589,574 (GRCm39)	missense	probably benign	0.35
R6372:Vmn2r65	UTSW	7	84,589,861 (GRCm39)	missense	probably damaging	1.00
R6924:Vmn2r65	UTSW	7	84,613,198 (GRCm39)	missense	probably benign	0.20
R7021:Vmn2r65	UTSW	7	84,596,587 (GRCm39)	missense	probably benign	0.00
R7195:Vmn2r65	UTSW	7	84,592,347 (GRCm39)	critical splice donor site	probably null
R7422:Vmn2r65	UTSW	7	84,595,569 (GRCm39)	missense	probably damaging	0.99
R7654:Vmn2r65	UTSW	7	84,590,261 (GRCm39)	missense	probably damaging	1.00
R7686:Vmn2r65	UTSW	7	84,589,744 (GRCm39)	missense	probably damaging	0.99
R7691:Vmn2r65	UTSW	7	84,592,851 (GRCm39)	missense	probably benign	0.30
R7798:Vmn2r65	UTSW	7	84,596,192 (GRCm39)	missense	probably damaging	1.00
R7798:Vmn2r65	UTSW	7	84,595,530 (GRCm39)	missense	probably benign	0.00
R8103:Vmn2r65	UTSW	7	84,595,919 (GRCm39)	missense	probably damaging	1.00
R8272:Vmn2r65	UTSW	7	84,596,817 (GRCm39)	missense	probably benign	0.02
R8303:Vmn2r65	UTSW	7	84,589,391 (GRCm39)	nonsense	probably null
R8354:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8454:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8489:Vmn2r65	UTSW	7	84,589,964 (GRCm39)	missense	possibly damaging	0.85
R8680:Vmn2r65	UTSW	7	84,589,388 (GRCm39)	missense	probably benign	0.00
R8731:Vmn2r65	UTSW	7	84,589,447 (GRCm39)	nonsense	probably null
R8839:Vmn2r65	UTSW	7	84,595,489 (GRCm39)	nonsense	probably null
R8847:Vmn2r65	UTSW	7	84,590,212 (GRCm39)	missense	probably damaging	1.00
R8916:Vmn2r65	UTSW	7	84,595,665 (GRCm39)	missense	probably benign	0.21
R9254:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9379:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9392:Vmn2r65	UTSW	7	84,589,718 (GRCm39)	missense	possibly damaging	0.95
R9563:Vmn2r65	UTSW	7	84,589,880 (GRCm39)	nonsense	probably null
R9686:Vmn2r65	UTSW	7	84,590,084 (GRCm39)	missense	probably benign	0.45
X0067:Vmn2r65	UTSW	7	84,590,113 (GRCm39)	missense	probably benign	0.04
Z1088:Vmn2r65	UTSW	7	84,592,473 (GRCm39)	critical splice acceptor site	probably null
Z1177:Vmn2r65	UTSW	7	84,590,234 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- GTGTTCTCATCCCAATGTGAGG -3'
(R):5'- ATCCTCTCCTAAGTGACCGG -3'

Sequencing Primer
(F):5'- CATCCCAATGTGAGGTGGTAATCC -3'
(R):5'- GGGAACACTATCCTTATCTGTACCAG -3'

Posted On

2021-01-18