Incidental Mutation 'R8899:Zfp831'
| ID |
679815 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp831
|
| Ensembl Gene |
ENSMUSG00000050600 |
| Gene Name |
zinc finger protein 831 |
| Synonyms |
ENSMUSG00000050600, OTTMUSG00000017459 |
| MMRRC Submission |
068756-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R8899 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
2 |
| Chromosomal Location |
174485327-174552625 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 174485978 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Tryptophan
at position 218
(R218W)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000060255
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000059452]
|
| AlphaFold |
A2ADM8 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000059452
AA Change: R218W
PolyPhen 2
Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000060255
Gene: ENSMUSG00000050600
AA Change: R218W
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
120 |
135 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
143 |
165 |
5.06e-2 |
SMART |
|
ZnF_C2H2
|
171 |
195 |
7.78e-3 |
SMART |
|
low complexity region
|
201 |
216 |
N/A |
INTRINSIC |
|
low complexity region
|
237 |
248 |
N/A |
INTRINSIC |
|
low complexity region
|
345 |
371 |
N/A |
INTRINSIC |
|
low complexity region
|
383 |
392 |
N/A |
INTRINSIC |
|
low complexity region
|
447 |
459 |
N/A |
INTRINSIC |
|
low complexity region
|
645 |
657 |
N/A |
INTRINSIC |
|
low complexity region
|
717 |
736 |
N/A |
INTRINSIC |
|
low complexity region
|
856 |
870 |
N/A |
INTRINSIC |
|
low complexity region
|
1520 |
1529 |
N/A |
INTRINSIC |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.5%
- 20x: 98.4%
|
| Validation Efficiency |
100% (70/70) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 70 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Agxt2 |
A |
T |
15: 10,378,900 (GRCm39) |
N167I |
probably damaging |
Het |
| Arel1 |
A |
G |
12: 84,981,017 (GRCm39) |
I330T |
probably benign |
Het |
| Ate1 |
T |
C |
7: 129,996,389 (GRCm39) |
K484E |
possibly damaging |
Het |
| Atl2 |
T |
C |
17: 80,183,469 (GRCm39) |
D42G |
probably benign |
Het |
| Bltp1 |
T |
C |
3: 37,042,429 (GRCm39) |
I2805T |
probably damaging |
Het |
| Cd209e |
C |
T |
8: 3,901,212 (GRCm39) |
W147* |
probably null |
Het |
| Cdc40 |
G |
A |
10: 40,717,809 (GRCm39) |
Q365* |
probably null |
Het |
| Cenpe |
A |
G |
3: 134,945,644 (GRCm39) |
T1053A |
probably benign |
Het |
| Colgalt1 |
T |
C |
8: 72,076,306 (GRCm39) |
S586P |
probably damaging |
Het |
| Crem |
A |
G |
18: 3,295,370 (GRCm39) |
I91T |
probably damaging |
Het |
| Csrnp3 |
G |
A |
2: 65,852,987 (GRCm39) |
V460I |
possibly damaging |
Het |
| Cul1 |
T |
A |
6: 47,474,246 (GRCm39) |
I136N |
possibly damaging |
Het |
| Ddx19b |
A |
T |
8: 111,737,929 (GRCm39) |
I273N |
probably damaging |
Het |
| Fap |
A |
G |
2: 62,348,817 (GRCm39) |
I505T |
probably damaging |
Het |
| Fbxo44 |
A |
T |
4: 148,238,078 (GRCm39) |
Y216* |
probably null |
Het |
| Fbxw10 |
T |
A |
11: 62,748,567 (GRCm39) |
M398K |
probably damaging |
Het |
| Fut10 |
T |
C |
8: 31,726,514 (GRCm39) |
V423A |
possibly damaging |
Het |
| Fyco1 |
A |
C |
9: 123,655,646 (GRCm39) |
D1037E |
probably benign |
Het |
| Gabrg2 |
G |
A |
11: 41,867,377 (GRCm39) |
R81* |
probably null |
Het |
| Gcn1 |
T |
C |
5: 115,717,220 (GRCm39) |
V204A |
probably benign |
Het |
| Gnl1 |
T |
C |
17: 36,299,608 (GRCm39) |
L593P |
probably damaging |
Het |
| Grm4 |
T |
C |
17: 27,653,754 (GRCm39) |
Q732R |
probably damaging |
Het |
| Icam5 |
T |
C |
9: 20,948,415 (GRCm39) |
V741A |
possibly damaging |
Het |
| Iqub |
C |
T |
6: 24,505,768 (GRCm39) |
E47K |
probably benign |
Het |
| Kcnk3 |
A |
G |
5: 30,779,580 (GRCm39) |
K210R |
probably benign |
Het |
| Kdm2b |
T |
A |
5: 123,125,851 (GRCm39) |
R12* |
probably null |
Het |
| Kifbp |
T |
C |
10: 62,399,282 (GRCm39) |
|
probably benign |
Het |
| Kndc1 |
C |
T |
7: 139,507,708 (GRCm39) |
S1222F |
possibly damaging |
Het |
| Lgi1 |
C |
T |
19: 38,294,538 (GRCm39) |
H413Y |
probably damaging |
Het |
| Lhx8 |
A |
G |
3: 154,033,653 (GRCm39) |
Y51H |
probably damaging |
Het |
| Lim2 |
T |
C |
7: 43,083,055 (GRCm39) |
I80T |
probably benign |
Het |
| Macf1 |
A |
G |
4: 123,368,852 (GRCm39) |
F405L |
probably benign |
Het |
| Mefv |
G |
A |
16: 3,528,764 (GRCm39) |
T559I |
probably damaging |
Het |
| Nin |
A |
G |
12: 70,077,710 (GRCm39) |
W1739R |
probably damaging |
Het |
| Or10h1 |
A |
T |
17: 33,418,718 (GRCm39) |
K232M |
probably damaging |
Het |
| Or11j4 |
T |
C |
14: 50,630,269 (GRCm39) |
F19L |
probably damaging |
Het |
| Or5t16 |
A |
G |
2: 86,818,710 (GRCm39) |
I270T |
probably benign |
Het |
| Or8b3b |
C |
T |
9: 38,584,147 (GRCm39) |
V198I |
probably damaging |
Het |
| Otop3 |
T |
C |
11: 115,231,886 (GRCm39) |
|
probably null |
Het |
| Pate9 |
A |
T |
9: 36,446,254 (GRCm39) |
C53S |
probably damaging |
Het |
| Pip5kl1 |
A |
G |
2: 32,469,082 (GRCm39) |
I247V |
probably benign |
Het |
| Polr2d |
T |
A |
18: 31,922,226 (GRCm39) |
M1K |
probably null |
Het |
| Prss53 |
T |
C |
7: 127,488,193 (GRCm39) |
T141A |
possibly damaging |
Het |
| Rdh12 |
A |
G |
12: 79,268,802 (GRCm39) |
N293S |
probably benign |
Het |
| Rnf146 |
G |
A |
10: 29,223,754 (GRCm39) |
T44I |
probably benign |
Het |
| Rsbn1l |
A |
T |
5: 21,101,865 (GRCm39) |
C588S |
probably damaging |
Het |
| Septin8 |
G |
A |
11: 53,426,862 (GRCm39) |
V208I |
probably damaging |
Het |
| Sgo2a |
T |
G |
1: 58,058,822 (GRCm39) |
S1134A |
possibly damaging |
Het |
| Sh3bp4 |
C |
T |
1: 89,073,297 (GRCm39) |
T715I |
probably benign |
Het |
| Snrnp200 |
A |
G |
2: 127,078,517 (GRCm39) |
T1758A |
probably damaging |
Het |
| Snx6 |
A |
T |
12: 54,812,423 (GRCm39) |
D70E |
probably benign |
Het |
| Spag9 |
T |
C |
11: 93,983,695 (GRCm39) |
S341P |
probably damaging |
Het |
| Sparcl1 |
T |
C |
5: 104,240,590 (GRCm39) |
D278G |
probably benign |
Het |
| Spmap2l |
T |
A |
5: 77,185,200 (GRCm39) |
|
probably null |
Het |
| Srbd1 |
T |
A |
17: 86,292,885 (GRCm39) |
S895C |
|
Het |
| Stxbp5l |
ATTTT |
ATTTTT |
16: 37,036,414 (GRCm39) |
|
probably null |
Het |
| Tas2r136 |
T |
C |
6: 132,754,323 (GRCm39) |
D268G |
probably benign |
Het |
| Tas2r143 |
T |
A |
6: 42,377,888 (GRCm39) |
Y239* |
probably null |
Het |
| Tbc1d17 |
C |
T |
7: 44,492,328 (GRCm39) |
G419D |
probably damaging |
Het |
| Tff2 |
C |
T |
17: 31,362,113 (GRCm39) |
W68* |
probably null |
Het |
| Thop1 |
T |
C |
10: 80,916,440 (GRCm39) |
C483R |
probably damaging |
Het |
| Tmem245 |
A |
C |
4: 56,903,916 (GRCm39) |
|
probably null |
Het |
| Tmem67 |
A |
G |
4: 12,055,038 (GRCm39) |
F655S |
probably damaging |
Het |
| Trim36 |
T |
G |
18: 46,302,264 (GRCm39) |
S583R |
possibly damaging |
Het |
| Ucp1 |
T |
C |
8: 84,017,216 (GRCm39) |
V2A |
probably benign |
Het |
| Ugt1a6a |
T |
A |
1: 88,066,803 (GRCm39) |
M203K |
probably damaging |
Het |
| Usp1 |
A |
G |
4: 98,819,347 (GRCm39) |
K270E |
probably damaging |
Het |
| Vps13c |
T |
C |
9: 67,841,783 (GRCm39) |
F1935S |
probably damaging |
Het |
| Zfp521 |
C |
A |
18: 13,979,137 (GRCm39) |
L425F |
probably damaging |
Het |
| Zfp799 |
G |
T |
17: 33,039,348 (GRCm39) |
P306Q |
probably damaging |
Het |
|
| Other mutations in Zfp831 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00089:Zfp831
|
APN |
2 |
174,488,078 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
IGL00091:Zfp831
|
APN |
2 |
174,487,451 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
IGL00764:Zfp831
|
APN |
2 |
174,487,701 (GRCm39) |
missense |
possibly damaging |
0.72 |
|
IGL01538:Zfp831
|
APN |
2 |
174,486,399 (GRCm39) |
missense |
possibly damaging |
0.72 |
|
IGL01700:Zfp831
|
APN |
2 |
174,486,711 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
IGL01718:Zfp831
|
APN |
2 |
174,485,631 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
IGL02221:Zfp831
|
APN |
2 |
174,485,519 (GRCm39) |
missense |
probably benign |
0.33 |
|
IGL02250:Zfp831
|
APN |
2 |
174,489,994 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
IGL03209:Zfp831
|
APN |
2 |
174,487,059 (GRCm39) |
missense |
probably benign |
0.40 |
|
D4043:Zfp831
|
UTSW |
2 |
174,487,059 (GRCm39) |
missense |
probably benign |
0.40 |
|
FR4304:Zfp831
|
UTSW |
2 |
174,487,274 (GRCm39) |
small insertion |
probably benign |
|
|
FR4340:Zfp831
|
UTSW |
2 |
174,487,273 (GRCm39) |
small insertion |
probably benign |
|
|
FR4449:Zfp831
|
UTSW |
2 |
174,487,275 (GRCm39) |
small insertion |
probably benign |
|
|
FR4449:Zfp831
|
UTSW |
2 |
174,487,264 (GRCm39) |
small insertion |
probably benign |
|
|
FR4589:Zfp831
|
UTSW |
2 |
174,487,261 (GRCm39) |
small insertion |
probably benign |
|
|
FR4737:Zfp831
|
UTSW |
2 |
174,487,276 (GRCm39) |
small insertion |
probably benign |
|
|
FR4737:Zfp831
|
UTSW |
2 |
174,487,269 (GRCm39) |
small insertion |
probably benign |
|
|
FR4737:Zfp831
|
UTSW |
2 |
174,487,264 (GRCm39) |
small insertion |
probably benign |
|
|
IGL02802:Zfp831
|
UTSW |
2 |
174,486,945 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
P0028:Zfp831
|
UTSW |
2 |
174,487,139 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
PIT4531001:Zfp831
|
UTSW |
2 |
174,488,516 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R0631:Zfp831
|
UTSW |
2 |
174,487,083 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R0644:Zfp831
|
UTSW |
2 |
174,487,656 (GRCm39) |
missense |
probably benign |
0.33 |
|
R0782:Zfp831
|
UTSW |
2 |
174,488,423 (GRCm39) |
missense |
probably benign |
0.06 |
|
R1156:Zfp831
|
UTSW |
2 |
174,488,710 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R1280:Zfp831
|
UTSW |
2 |
174,545,852 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1709:Zfp831
|
UTSW |
2 |
174,487,683 (GRCm39) |
missense |
probably benign |
0.33 |
|
R1883:Zfp831
|
UTSW |
2 |
174,545,870 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R1884:Zfp831
|
UTSW |
2 |
174,545,870 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R2127:Zfp831
|
UTSW |
2 |
174,489,917 (GRCm39) |
missense |
probably benign |
0.33 |
|
R2137:Zfp831
|
UTSW |
2 |
174,547,539 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R2268:Zfp831
|
UTSW |
2 |
174,486,034 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2330:Zfp831
|
UTSW |
2 |
174,489,882 (GRCm39) |
nonsense |
probably null |
|
|
R3547:Zfp831
|
UTSW |
2 |
174,499,476 (GRCm39) |
missense |
probably benign |
|
|
R3821:Zfp831
|
UTSW |
2 |
174,485,816 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R4163:Zfp831
|
UTSW |
2 |
174,485,822 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R4232:Zfp831
|
UTSW |
2 |
174,547,447 (GRCm39) |
missense |
possibly damaging |
0.96 |
|
R4778:Zfp831
|
UTSW |
2 |
174,488,600 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R4820:Zfp831
|
UTSW |
2 |
174,547,097 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R4912:Zfp831
|
UTSW |
2 |
174,486,417 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5119:Zfp831
|
UTSW |
2 |
174,547,103 (GRCm39) |
missense |
probably benign |
0.18 |
|
R5152:Zfp831
|
UTSW |
2 |
174,486,357 (GRCm39) |
missense |
probably benign |
0.33 |
|
R5723:Zfp831
|
UTSW |
2 |
174,487,200 (GRCm39) |
missense |
probably benign |
0.23 |
|
R5741:Zfp831
|
UTSW |
2 |
174,486,945 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R5888:Zfp831
|
UTSW |
2 |
174,485,420 (GRCm39) |
missense |
probably benign |
0.18 |
|
R5975:Zfp831
|
UTSW |
2 |
174,485,885 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R6092:Zfp831
|
UTSW |
2 |
174,547,299 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R6158:Zfp831
|
UTSW |
2 |
174,485,651 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R6212:Zfp831
|
UTSW |
2 |
174,487,661 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R6233:Zfp831
|
UTSW |
2 |
174,488,490 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R6248:Zfp831
|
UTSW |
2 |
174,486,308 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R6255:Zfp831
|
UTSW |
2 |
174,488,214 (GRCm39) |
missense |
possibly damaging |
0.96 |
|
R6460:Zfp831
|
UTSW |
2 |
174,488,360 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R6477:Zfp831
|
UTSW |
2 |
174,545,960 (GRCm39) |
missense |
probably benign |
|
|
R6864:Zfp831
|
UTSW |
2 |
174,488,533 (GRCm39) |
missense |
possibly damaging |
0.72 |
|
R7396:Zfp831
|
UTSW |
2 |
174,487,002 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R7447:Zfp831
|
UTSW |
2 |
174,487,896 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R7499:Zfp831
|
UTSW |
2 |
174,485,816 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R7662:Zfp831
|
UTSW |
2 |
174,487,934 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R7857:Zfp831
|
UTSW |
2 |
174,547,035 (GRCm39) |
missense |
probably benign |
0.33 |
|
R7889:Zfp831
|
UTSW |
2 |
174,487,097 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R7896:Zfp831
|
UTSW |
2 |
174,488,921 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R8074:Zfp831
|
UTSW |
2 |
174,486,528 (GRCm39) |
missense |
possibly damaging |
0.72 |
|
R8089:Zfp831
|
UTSW |
2 |
174,486,717 (GRCm39) |
missense |
possibly damaging |
0.96 |
|
R8438:Zfp831
|
UTSW |
2 |
174,486,796 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R8716:Zfp831
|
UTSW |
2 |
174,547,049 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R8757:Zfp831
|
UTSW |
2 |
174,487,874 (GRCm39) |
missense |
probably benign |
|
|
R8759:Zfp831
|
UTSW |
2 |
174,487,874 (GRCm39) |
missense |
probably benign |
|
|
R8976:Zfp831
|
UTSW |
2 |
174,487,079 (GRCm39) |
missense |
possibly damaging |
0.76 |
|
R9146:Zfp831
|
UTSW |
2 |
174,487,461 (GRCm39) |
missense |
possibly damaging |
0.72 |
|
R9257:Zfp831
|
UTSW |
2 |
174,488,156 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R9324:Zfp831
|
UTSW |
2 |
174,547,113 (GRCm39) |
missense |
probably benign |
0.33 |
|
R9467:Zfp831
|
UTSW |
2 |
174,486,789 (GRCm39) |
missense |
probably benign |
0.33 |
|
R9729:Zfp831
|
UTSW |
2 |
174,487,938 (GRCm39) |
missense |
possibly damaging |
0.96 |
|
X0021:Zfp831
|
UTSW |
2 |
174,547,662 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
Z1177:Zfp831
|
UTSW |
2 |
174,485,981 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
| Predicted Primers |
PCR Primer
(F):5'- ACTGCTTGAAGCCCAGTGTG -3'
(R):5'- CTAGTGAAAACTCAGGTGACACC -3'
Sequencing Primer
(F):5'- TGCTGGAGAAACACATCCGGTC -3'
(R):5'- GTGACACCATCTGAGTGTGATCC -3'
|
| Posted On |
2021-08-31 |
Incidental Mutation