Incidental Mutation 'R1394:Pcdh12'
| ID |
162767 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Pcdh12
|
| Ensembl Gene |
ENSMUSG00000024440 |
| Gene Name |
protocadherin 12 |
| Synonyms |
VE-cadherin-2, vascular endothelial cadherin-2 |
| MMRRC Submission |
039456-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R1394 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
18 |
| Chromosomal Location |
38400145-38417454 bp(-) (GRCm39) |
| Type of Mutation |
critical splice donor site (2 bp from exon) |
| DNA Base Change (assembly) |
A to G
at 38414242 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000025311
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000025311]
[ENSMUST00000194012]
|
| AlphaFold |
O55134 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000025311
|
| SMART Domains |
Protein: ENSMUSP00000025311
Gene: ENSMUSG00000024440
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
17 |
N/A |
INTRINSIC |
|
CA
|
53 |
133 |
4.42e-2 |
SMART |
|
CA
|
157 |
242 |
2.55e-17 |
SMART |
|
CA
|
266 |
350 |
2.31e-24 |
SMART |
|
CA
|
376 |
458 |
3.86e-26 |
SMART |
|
CA
|
482 |
563 |
6.27e-26 |
SMART |
|
CA
|
621 |
704 |
3.02e-2 |
SMART |
|
transmembrane domain
|
716 |
738 |
N/A |
INTRINSIC |
|
low complexity region
|
960 |
975 |
N/A |
INTRINSIC |
|
low complexity region
|
1032 |
1041 |
N/A |
INTRINSIC |
|
low complexity region
|
1115 |
1125 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000193123
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000194012
|
| SMART Domains |
Protein: ENSMUSP00000141907
Gene: ENSMUSG00000024440
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
56 |
66 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000195747
|
| Coding Region Coverage |
- 1x: 98.9%
- 3x: 98.0%
- 10x: 95.3%
- 20x: 89.4%
|
| Validation Efficiency |
|
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 6 cadherin repeats, a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene localizes to the region on chromosome 5 where the protocadherin gene clusters reside. The exon organization of this transcript is similar to that of the gene cluster transcripts, notably the first large exon, but no significant sequence homology exists. The function of this cellular adhesion protein is undetermined but mouse protocadherin 12 does not bind catenins and appears to have no affect on cell migration or growth. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a targeted mutation are viable, fertile and do not display any obvious histomorphological abnormalities. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 48 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 2900026A02Rik |
A |
G |
5: 113,249,362 (GRCm39) |
Y122H |
probably damaging |
Het |
| Ankrd27 |
T |
C |
7: 35,315,294 (GRCm39) |
F481S |
possibly damaging |
Het |
| Casd1 |
T |
G |
6: 4,624,117 (GRCm39) |
C303W |
probably damaging |
Het |
| Cep128 |
C |
T |
12: 91,233,754 (GRCm39) |
R438Q |
probably benign |
Het |
| Cep192 |
A |
G |
18: 67,991,992 (GRCm39) |
T1957A |
probably damaging |
Het |
| Cep290 |
T |
C |
10: 100,373,391 (GRCm39) |
S1224P |
possibly damaging |
Het |
| Col5a2 |
A |
G |
1: 45,442,579 (GRCm39) |
|
probably null |
Het |
| Cwc22 |
G |
A |
2: 77,759,823 (GRCm39) |
R75C |
possibly damaging |
Het |
| Cyp4a30b |
A |
T |
4: 115,328,089 (GRCm39) |
|
probably null |
Het |
| Dnah11 |
T |
C |
12: 117,936,099 (GRCm39) |
D3298G |
possibly damaging |
Het |
| Drc3 |
T |
C |
11: 60,284,545 (GRCm39) |
I450T |
possibly damaging |
Het |
| Dst |
T |
C |
1: 34,204,236 (GRCm39) |
|
probably null |
Het |
| Dync1h1 |
G |
A |
12: 110,602,943 (GRCm39) |
E2195K |
probably benign |
Het |
| Emilin2 |
G |
T |
17: 71,560,066 (GRCm39) |
D970E |
possibly damaging |
Het |
| Fcgbp |
A |
G |
7: 27,792,804 (GRCm39) |
H936R |
probably damaging |
Het |
| Fkbp15 |
T |
A |
4: 62,246,109 (GRCm39) |
M440L |
probably benign |
Het |
| Fryl |
T |
C |
5: 73,230,255 (GRCm39) |
H1634R |
probably damaging |
Het |
| Gm44511 |
G |
A |
6: 128,797,293 (GRCm39) |
S32L |
possibly damaging |
Het |
| Gtf3c3 |
C |
T |
1: 54,456,937 (GRCm39) |
A488T |
probably damaging |
Het |
| Ift81 |
G |
T |
5: 122,706,986 (GRCm39) |
D485E |
probably benign |
Het |
| Ipp |
T |
A |
4: 116,395,109 (GRCm39) |
L548* |
probably null |
Het |
| Itm2a |
C |
T |
X: 106,441,807 (GRCm39) |
V200I |
possibly damaging |
Het |
| Kank1 |
A |
G |
19: 25,405,528 (GRCm39) |
N1182S |
probably damaging |
Het |
| Mkks |
C |
T |
2: 136,722,882 (GRCm39) |
G92S |
probably damaging |
Het |
| Mybbp1a |
C |
T |
11: 72,334,474 (GRCm39) |
P243L |
probably damaging |
Het |
| Myo1f |
A |
G |
17: 33,802,714 (GRCm39) |
D386G |
probably damaging |
Het |
| Obsl1 |
T |
C |
1: 75,469,309 (GRCm39) |
S109G |
probably damaging |
Het |
| Or6z6 |
T |
A |
7: 6,491,361 (GRCm39) |
T171S |
probably damaging |
Het |
| Or9s13 |
T |
A |
1: 92,548,267 (GRCm39) |
I213N |
probably benign |
Het |
| Phlpp1 |
T |
C |
1: 106,278,348 (GRCm39) |
V920A |
possibly damaging |
Het |
| Phlpp2 |
T |
A |
8: 110,603,662 (GRCm39) |
C109* |
probably null |
Het |
| Prickle2 |
T |
A |
6: 92,353,363 (GRCm39) |
H701L |
possibly damaging |
Het |
| Psen1 |
G |
A |
12: 83,771,346 (GRCm39) |
G209R |
probably damaging |
Het |
| Psg19 |
T |
C |
7: 18,530,983 (GRCm39) |
N57S |
probably damaging |
Het |
| Rdh12 |
A |
G |
12: 79,255,839 (GRCm39) |
T9A |
probably benign |
Het |
| Rgma |
G |
T |
7: 73,067,542 (GRCm39) |
A360S |
probably benign |
Het |
| Scyl2 |
T |
A |
10: 89,476,827 (GRCm39) |
K766M |
possibly damaging |
Het |
| Sned1 |
G |
A |
1: 93,209,376 (GRCm39) |
V830M |
possibly damaging |
Het |
| Spata31e5 |
T |
C |
1: 28,815,890 (GRCm39) |
E714G |
possibly damaging |
Het |
| Spata46 |
A |
G |
1: 170,139,573 (GRCm39) |
T191A |
probably benign |
Het |
| Spin1 |
T |
C |
13: 51,298,517 (GRCm39) |
Y179H |
probably damaging |
Het |
| Tecpr1 |
T |
C |
5: 144,143,357 (GRCm39) |
T673A |
possibly damaging |
Het |
| Tenm3 |
T |
A |
8: 48,729,435 (GRCm39) |
M1508L |
probably benign |
Het |
| Vasn |
C |
T |
16: 4,467,576 (GRCm39) |
R508* |
probably null |
Het |
| Vmn2r15 |
T |
A |
5: 109,442,014 (GRCm39) |
I140L |
probably benign |
Het |
| Wdr44 |
T |
G |
X: 23,662,298 (GRCm39) |
C645G |
probably damaging |
Het |
| Zfand1 |
G |
T |
3: 10,411,269 (GRCm39) |
T62K |
probably benign |
Het |
| Zfy1 |
C |
T |
Y: 725,957 (GRCm39) |
V603I |
possibly damaging |
Het |
|
| Other mutations in Pcdh12 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00898:Pcdh12
|
APN |
18 |
38,414,510 (GRCm39) |
missense |
probably benign |
|
|
IGL00964:Pcdh12
|
APN |
18 |
38,415,784 (GRCm39) |
missense |
probably benign |
0.27 |
|
IGL01105:Pcdh12
|
APN |
18 |
38,408,400 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02011:Pcdh12
|
APN |
18 |
38,414,473 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02234:Pcdh12
|
APN |
18 |
38,416,588 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02452:Pcdh12
|
APN |
18 |
38,414,746 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL03412:Pcdh12
|
APN |
18 |
38,416,568 (GRCm39) |
missense |
probably benign |
0.24 |
|
R0729:Pcdh12
|
UTSW |
18 |
38,415,517 (GRCm39) |
missense |
probably benign |
0.20 |
|
R1330:Pcdh12
|
UTSW |
18 |
38,414,914 (GRCm39) |
missense |
probably benign |
0.13 |
|
R1413:Pcdh12
|
UTSW |
18 |
38,416,496 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1993:Pcdh12
|
UTSW |
18 |
38,415,196 (GRCm39) |
missense |
possibly damaging |
0.62 |
|
R2115:Pcdh12
|
UTSW |
18 |
38,417,039 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2567:Pcdh12
|
UTSW |
18 |
38,415,149 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2926:Pcdh12
|
UTSW |
18 |
38,415,443 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R3810:Pcdh12
|
UTSW |
18 |
38,414,290 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3813:Pcdh12
|
UTSW |
18 |
38,416,667 (GRCm39) |
nonsense |
probably null |
|
|
R5275:Pcdh12
|
UTSW |
18 |
38,417,154 (GRCm39) |
utr 5 prime |
probably benign |
|
|
R5400:Pcdh12
|
UTSW |
18 |
38,401,951 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5523:Pcdh12
|
UTSW |
18 |
38,416,192 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5539:Pcdh12
|
UTSW |
18 |
38,414,797 (GRCm39) |
missense |
possibly damaging |
0.77 |
|
R5604:Pcdh12
|
UTSW |
18 |
38,401,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6012:Pcdh12
|
UTSW |
18 |
38,416,805 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6042:Pcdh12
|
UTSW |
18 |
38,414,558 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6129:Pcdh12
|
UTSW |
18 |
38,410,912 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6239:Pcdh12
|
UTSW |
18 |
38,415,454 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6508:Pcdh12
|
UTSW |
18 |
38,414,390 (GRCm39) |
nonsense |
probably null |
|
|
R7250:Pcdh12
|
UTSW |
18 |
38,415,029 (GRCm39) |
missense |
probably benign |
|
|
R7259:Pcdh12
|
UTSW |
18 |
38,414,677 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7271:Pcdh12
|
UTSW |
18 |
38,416,100 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7489:Pcdh12
|
UTSW |
18 |
38,414,842 (GRCm39) |
missense |
possibly damaging |
0.77 |
|
R8103:Pcdh12
|
UTSW |
18 |
38,415,212 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8157:Pcdh12
|
UTSW |
18 |
38,415,850 (GRCm39) |
missense |
probably benign |
|
|
R8322:Pcdh12
|
UTSW |
18 |
38,414,630 (GRCm39) |
nonsense |
probably null |
|
|
R8471:Pcdh12
|
UTSW |
18 |
38,415,308 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8503:Pcdh12
|
UTSW |
18 |
38,415,574 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R8510:Pcdh12
|
UTSW |
18 |
38,415,109 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R8677:Pcdh12
|
UTSW |
18 |
38,415,191 (GRCm39) |
missense |
probably benign |
0.01 |
|
R8788:Pcdh12
|
UTSW |
18 |
38,416,109 (GRCm39) |
missense |
probably benign |
0.19 |
|
R9274:Pcdh12
|
UTSW |
18 |
38,415,950 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9639:Pcdh12
|
UTSW |
18 |
38,402,032 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9697:Pcdh12
|
UTSW |
18 |
38,415,022 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
Z1177:Pcdh12
|
UTSW |
18 |
38,416,045 (GRCm39) |
missense |
probably benign |
0.01 |
|
| Predicted Primers |
PCR Primer
(F):5'- TTTCCTAATGCAGATGTGGGAGAAGC -3'
(R):5'- CGGGAGAACCTAAACCTTCCTGAGTC -3'
Sequencing Primer
(F):5'- tggtgggaagaggtggag -3'
(R):5'- TGGTAGCCCCATAGCGAG -3'
|
| Posted On |
2014-03-17 |
Incidental Mutation