Mutagenetix > Incidental Mutation

Incidental Mutation 'R1782:Trrap'

195379

Institutional Source

Beutler Lab

Gene Symbol

Trrap

Ensembl Gene

ENSMUSG00000045482

Gene Name

transformation/transcription domain-associated protein

Synonyms

transactivation/transformation-domain associated protein

MMRRC Submission

039813-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1782 (G1)

Quality Score

176

Status

Not validated

Chromosome

Chromosomal Location

144704547-144796588 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 144759513 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 2231 (V2231A)

Ref Sequence

ENSEMBL: ENSMUSP00000148419 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038980] [ENSMUST00000094120] [ENSMUST00000100467] [ENSMUST00000213013]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000038980
AA Change: V2212A

PolyPhen 2 Score 0.770 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000042544
Gene: ENSMUSG00000045482
AA Change: V2212A

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1664	2e-6	SMART
low complexity region	1832	1843	N/A	INTRINSIC
low complexity region	1866	1881	N/A	INTRINSIC
low complexity region	2289	2303	N/A	INTRINSIC
Pfam:FAT	2830	3174	4.7e-69	PFAM
low complexity region	3363	3376	N/A	INTRINSIC
low complexity region	3407	3418	N/A	INTRINSIC
PI3Kc	3509	3798	5.11e-8	SMART
FATC	3797	3829	1.89e-3	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094120
AA Change: V2230A

PolyPhen 2 Score 0.880 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000091668
Gene: ENSMUSG00000045482
AA Change: V2230A

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1682	2e-6	SMART
low complexity region	1850	1861	N/A	INTRINSIC
low complexity region	1884	1899	N/A	INTRINSIC
low complexity region	2307	2321	N/A	INTRINSIC
Pfam:FAT	2848	3203	1.1e-68	PFAM
low complexity region	3392	3405	N/A	INTRINSIC
low complexity region	3436	3447	N/A	INTRINSIC
PI3Kc	3538	3827	5.11e-8	SMART
FATC	3826	3858	1.89e-3	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000100467
AA Change: V2212A

PolyPhen 2 Score 0.883 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000098035
Gene: ENSMUSG00000045482
AA Change: V2212A

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1664	2e-6	SMART
low complexity region	1832	1843	N/A	INTRINSIC
low complexity region	1866	1881	N/A	INTRINSIC
low complexity region	2289	2303	N/A	INTRINSIC
Pfam:FAT	2830	3174	4.7e-69	PFAM
low complexity region	3381	3394	N/A	INTRINSIC
low complexity region	3425	3436	N/A	INTRINSIC
PI3Kc	3527	3816	5.11e-8	SMART
FATC	3815	3847	1.89e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132347

Predicted Effect

unknown
Transcript: ENSMUST00000132925
AA Change: V1951A

SMART Domains

Protein: ENSMUSP00000122021
Gene: ENSMUSG00000045482
AA Change: V1951A

Domain	Start	End	E-Value	Type
low complexity region	197	242	N/A	INTRINSIC
low complexity region	244	255	N/A	INTRINSIC
SCOP:d1gw5a_	474	1003	9e-7	SMART
Blast:PI3Kc	480	579	1e-13	BLAST
low complexity region	1083	1092	N/A	INTRINSIC
low complexity region	1572	1583	N/A	INTRINSIC
low complexity region	1606	1621	N/A	INTRINSIC
low complexity region	2029	2043	N/A	INTRINSIC
Pfam:FAT	2570	2914	1.5e-69	PFAM
low complexity region	3121	3134	N/A	INTRINSIC
low complexity region	3165	3176	N/A	INTRINSIC
PI3Kc	3267	3556	5.11e-8	SMART
FATC	3555	3587	1.89e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143357

Predicted Effect

possibly damaging
Transcript: ENSMUST00000213013
AA Change: V2231A

PolyPhen 2 Score 0.928 (Sensitivity: 0.81; Specificity: 0.94)

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.2%
20x: 92.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a large multidomain protein of the phosphoinositide 3-kinase-related kinases (PIKK) family. The encoded protein is a common component of many histone acetyltransferase (HAT) complexes and plays a role in transcription and DNA repair by recruiting HAT complexes to chromatin. Deregulation of this gene may play a role in several types of cancer including glioblastoma multiforme. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
PHENOTYPE: Homozygous embryos die prior to E3.5 and exhibit embryonic and extraembryonic tissue disorganization. Mitotic abnormalities were also noted in homozygous cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	T	11: 9,247,971 (GRCm39)	T2573S	probably benign	Het
Adgra3	G	T	5: 50,129,404 (GRCm39)	T738K	probably benign	Het
Adgrl4	C	T	3: 151,248,442 (GRCm39)	Q705*	probably null	Het
Atp2b1	T	G	10: 98,839,063 (GRCm39)	D630E	probably benign	Het
Atp2c1	G	A	9: 105,308,786 (GRCm39)	R577C	probably damaging	Het
Atp9b	A	G	18: 80,809,137 (GRCm39)	V211A	probably damaging	Het
C8g	T	A	2: 25,389,094 (GRCm39)	D163V	possibly damaging	Het
Catsper1	T	C	19: 5,385,937 (GRCm39)	Y57H	probably benign	Het
Ccdc25	C	T	14: 66,091,597 (GRCm39)	A72V	probably benign	Het
Cdca2	T	G	14: 67,915,260 (GRCm39)	E666D	probably benign	Het
Cdh23	G	A	10: 60,324,321 (GRCm39)	T313I	probably damaging	Het
Cfap57	C	A	4: 118,472,172 (GRCm39)	R69L	probably damaging	Het
Chat	C	A	14: 32,130,944 (GRCm39)	V566L	probably damaging	Het
Cntn3	T	C	6: 102,250,772 (GRCm39)	I259V	probably damaging	Het
Cog1	A	G	11: 113,544,792 (GRCm39)	T325A	probably benign	Het
Cxcl10	A	G	5: 92,495,662 (GRCm39)	*94Q	probably null	Het
Cyp4a12b	T	C	4: 115,291,178 (GRCm39)	Y369H	probably damaging	Het
Dhx33	T	C	11: 70,892,466 (GRCm39)	Y101C	probably damaging	Het
Dock6	A	G	9: 21,723,142 (GRCm39)	M1593T	probably damaging	Het
Dpy19l3	T	C	7: 35,407,580 (GRCm39)	T488A	possibly damaging	Het
Elapor2	A	T	5: 9,471,620 (GRCm39)	K320N	possibly damaging	Het
Fbxw14	A	G	9: 109,107,759 (GRCm39)	I205T	possibly damaging	Het
Fbxw7	T	C	3: 84,811,126 (GRCm39)	F84L	probably benign	Het
Flii	T	C	11: 60,605,462 (GRCm39)	T1212A	probably benign	Het
Fosl1	T	A	19: 5,500,210 (GRCm39)	I43N	probably damaging	Het
Gabrr2	G	A	4: 33,085,593 (GRCm39)	A338T	probably damaging	Het
Gask1b	T	C	3: 79,793,838 (GRCm39)	L102S	possibly damaging	Het
Gatad2b	T	C	3: 90,249,178 (GRCm39)	V72A	probably benign	Het
Gorasp1	G	T	9: 119,761,888 (GRCm39)	N48K	probably damaging	Het
Gramd1b	C	A	9: 40,324,633 (GRCm39)	D139Y	probably damaging	Het
Gtf3c1	A	T	7: 125,266,246 (GRCm39)	V1030E	probably damaging	Het
Havcr2	C	T	11: 46,345,844 (GRCm39)	T6I	unknown	Het
Hgs	A	G	11: 120,369,331 (GRCm39)	E340G	probably damaging	Het
Irx2	A	G	13: 72,779,585 (GRCm39)	T290A	probably benign	Het
Itgb8	T	A	12: 119,155,853 (GRCm39)	I200F	probably damaging	Het
Josd2	A	G	7: 44,120,577 (GRCm39)	I105V	probably damaging	Het
Kcnh7	T	A	2: 62,566,513 (GRCm39)	D806V	probably damaging	Het
Kctd8	A	G	5: 69,498,319 (GRCm39)	V109A	possibly damaging	Het
Kmt2d	T	A	15: 98,755,429 (GRCm39)		probably benign	Het
Krtap2-4	A	T	11: 99,505,353 (GRCm39)	V86E	probably damaging	Het
Lgr6	C	G	1: 134,915,717 (GRCm39)	V344L	probably damaging	Het
Lime1	A	T	2: 181,024,849 (GRCm39)	R168W	possibly damaging	Het
Macroh2a1	A	T	13: 56,222,134 (GRCm39)	M339K	probably damaging	Het
Magel2	C	T	7: 62,030,605 (GRCm39)	Q1170*	probably null	Het
Ndufaf3	A	T	9: 108,443,210 (GRCm39)	I169N	probably damaging	Het
Neb	T	A	2: 52,174,357 (GRCm39)	K1501*	probably null	Het
Nim1k	A	T	13: 120,173,687 (GRCm39)	S402R	probably benign	Het
Nt5dc2	T	G	14: 30,860,158 (GRCm39)	S395R	probably damaging	Het
Oaz2	G	T	9: 65,596,143 (GRCm39)	V132L	probably benign	Het
Or2t45	T	A	11: 58,669,631 (GRCm39)	L226H	probably damaging	Het
Or4l1	A	T	14: 50,166,096 (GRCm39)	W302R	probably benign	Het
Or5b104	T	A	19: 13,072,167 (GRCm39)	I282F	probably damaging	Het
Or7g25	C	T	9: 19,159,877 (GRCm39)	V273I	probably benign	Het
Or8k39	T	C	2: 86,563,026 (GRCm39)	K310R	probably benign	Het
Pfkl	A	G	10: 77,824,554 (GRCm39)	V717A	probably benign	Het
Phf8-ps	T	C	17: 33,286,662 (GRCm39)	I47V	probably benign	Het
Phgdh	C	T	3: 98,228,063 (GRCm39)	V231I	probably damaging	Het
Pkhd1	A	T	1: 20,635,935 (GRCm39)	M465K	probably damaging	Het
Ppp3r1	A	G	11: 17,148,281 (GRCm39)	H163R	probably benign	Het
Prune2	T	A	19: 17,099,537 (GRCm39)	N1680K	probably benign	Het
Puf60	T	A	15: 75,943,724 (GRCm39)	I216L	probably benign	Het
Rev3l	T	A	10: 39,675,881 (GRCm39)	N190K	probably benign	Het
Rp1	G	A	1: 4,419,312 (GRCm39)	S600L	probably benign	Het
Rpl3l	A	G	17: 24,952,430 (GRCm39)	I217V	probably benign	Het
Scly	T	A	1: 91,236,102 (GRCm39)	V194D	probably damaging	Het
Scnn1b	A	G	7: 121,517,184 (GRCm39)	T607A	probably benign	Het
Slc13a3	G	A	2: 165,287,439 (GRCm39)	L172F	probably benign	Het
Sorbs2	A	G	8: 46,258,733 (GRCm39)	Y1090C	probably damaging	Het
Spag17	T	A	3: 99,918,070 (GRCm39)	M351K	probably benign	Het
St14	A	G	9: 31,011,460 (GRCm39)	Y444H	probably damaging	Het
Taf8	C	A	17: 47,809,136 (GRCm39)	A109S	probably benign	Het
Tbc1d30	T	A	10: 121,103,525 (GRCm39)	K502N	probably damaging	Het
Them5	T	C	3: 94,251,796 (GRCm39)	S136P	probably benign	Het
Tmem248	T	A	5: 130,260,769 (GRCm39)	N111K	probably damaging	Het
Tmem74	C	A	15: 43,730,348 (GRCm39)	V232L	probably damaging	Het
Tnip2	A	T	5: 34,657,012 (GRCm39)	H264Q	probably benign	Het
Trim5	A	G	7: 103,915,023 (GRCm39)		probably null	Het
Trim63	A	G	4: 134,050,349 (GRCm39)	Q211R	probably benign	Het
Ttn	T	C	2: 76,565,831 (GRCm39)	S28174G	probably benign	Het
Ugt2b36	T	C	5: 87,229,440 (GRCm39)	D341G	possibly damaging	Het
Uroc1	G	A	6: 90,313,901 (GRCm39)	E63K	probably damaging	Het
Ush2a	T	C	1: 188,643,382 (GRCm39)	V4248A	probably benign	Het
Usp1	A	G	4: 98,822,435 (GRCm39)	H583R	probably damaging	Het
Usp8	T	A	2: 126,561,971 (GRCm39)	F55Y	probably damaging	Het
Vmn2r13	T	A	5: 109,306,040 (GRCm39)	T513S	probably benign	Het
Wdr73	T	C	7: 80,541,526 (GRCm39)	T339A	probably damaging	Het
Wnt2	T	A	6: 18,008,639 (GRCm39)	N266I	possibly damaging	Het
Wwp2	AGAACT	A	8: 108,233,031 (GRCm39)		probably null	Het

Other mutations in Trrap

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Trrap	APN	5	144,716,784 (GRCm39)	splice site	probably benign
IGL00470:Trrap	APN	5	144,754,848 (GRCm39)	missense	probably damaging	1.00
IGL00490:Trrap	APN	5	144,762,035 (GRCm39)	missense	probably benign	0.40
IGL01072:Trrap	APN	5	144,721,065 (GRCm39)	splice site	probably benign
IGL01087:Trrap	APN	5	144,783,349 (GRCm39)	missense	probably damaging	0.99
IGL01300:Trrap	APN	5	144,741,628 (GRCm39)	missense	probably damaging	1.00
IGL01350:Trrap	APN	5	144,767,779 (GRCm39)	missense	possibly damaging	0.92
IGL01410:Trrap	APN	5	144,767,831 (GRCm39)	missense	probably benign	0.00
IGL01571:Trrap	APN	5	144,770,097 (GRCm39)	splice site	probably benign
IGL01748:Trrap	APN	5	144,770,150 (GRCm39)	missense	probably damaging	1.00
IGL01839:Trrap	APN	5	144,758,685 (GRCm39)	missense	probably damaging	1.00
IGL01976:Trrap	APN	5	144,793,799 (GRCm39)	missense	probably benign	0.00
IGL02075:Trrap	APN	5	144,765,304 (GRCm39)	missense	probably benign	0.00
IGL02127:Trrap	APN	5	144,753,243 (GRCm39)	missense	probably benign	0.22
IGL02131:Trrap	APN	5	144,777,246 (GRCm39)	missense	probably damaging	1.00
IGL02287:Trrap	APN	5	144,769,348 (GRCm39)	missense	probably damaging	1.00
IGL02301:Trrap	APN	5	144,714,727 (GRCm39)	missense	probably benign	0.05
IGL02336:Trrap	APN	5	144,735,200 (GRCm39)	missense	probably benign	0.39
IGL02526:Trrap	APN	5	144,761,360 (GRCm39)	missense	probably benign	0.00
IGL02873:Trrap	APN	5	144,777,889 (GRCm39)	splice site	probably benign
IGL02953:Trrap	APN	5	144,752,774 (GRCm39)	missense	probably damaging	0.99
IGL03404:Trrap	APN	5	144,769,996 (GRCm39)	missense	probably benign	0.00
Buffer	UTSW	5	144,771,014 (GRCm39)	missense	probably benign	0.06
Card-tower	UTSW	5	144,741,576 (GRCm39)	missense	probably damaging	1.00
Cookie	UTSW	5	144,730,859 (GRCm39)	missense	probably damaging	1.00
Glass_house	UTSW	5	144,782,287 (GRCm39)	missense	possibly damaging	0.67
Immovable	UTSW	5	144,727,665 (GRCm39)	missense	possibly damaging	0.66
R5049_trrap_520	UTSW	5	144,763,527 (GRCm39)	missense	probably damaging	1.00
R7167_Trrap_977	UTSW	5	144,776,424 (GRCm39)	missense	probably benign	0.39
vitreous	UTSW	5	144,742,537 (GRCm39)	missense	probably damaging	1.00
PIT4243001:Trrap	UTSW	5	144,733,781 (GRCm39)	missense	probably benign	0.00
PIT4466001:Trrap	UTSW	5	144,765,410 (GRCm39)	missense	probably benign	0.02
R0062:Trrap	UTSW	5	144,719,003 (GRCm39)	splice site	probably benign
R0062:Trrap	UTSW	5	144,719,003 (GRCm39)	splice site	probably benign
R0112:Trrap	UTSW	5	144,759,571 (GRCm39)	nonsense	probably null
R0126:Trrap	UTSW	5	144,742,560 (GRCm39)	nonsense	probably null
R0257:Trrap	UTSW	5	144,741,045 (GRCm39)	missense	probably benign	0.31
R0325:Trrap	UTSW	5	144,753,205 (GRCm39)	missense	probably benign	0.05
R0376:Trrap	UTSW	5	144,753,149 (GRCm39)	missense	probably benign	0.03
R0396:Trrap	UTSW	5	144,751,366 (GRCm39)	missense	probably damaging	0.99
R0448:Trrap	UTSW	5	144,776,377 (GRCm39)	missense	possibly damaging	0.66
R0454:Trrap	UTSW	5	144,783,287 (GRCm39)	missense	probably damaging	1.00
R0711:Trrap	UTSW	5	144,790,309 (GRCm39)	missense	probably damaging	1.00
R0827:Trrap	UTSW	5	144,751,640 (GRCm39)	missense	probably benign	0.00
R1005:Trrap	UTSW	5	144,742,537 (GRCm39)	missense	probably damaging	1.00
R1147:Trrap	UTSW	5	144,741,576 (GRCm39)	missense	probably damaging	1.00
R1147:Trrap	UTSW	5	144,741,576 (GRCm39)	missense	probably damaging	1.00
R1179:Trrap	UTSW	5	144,714,749 (GRCm39)	missense	possibly damaging	0.94
R1218:Trrap	UTSW	5	144,753,219 (GRCm39)	missense	probably damaging	1.00
R1264:Trrap	UTSW	5	144,726,409 (GRCm39)	splice site	probably benign
R1374:Trrap	UTSW	5	144,783,428 (GRCm39)	missense	probably damaging	1.00
R1401:Trrap	UTSW	5	144,794,232 (GRCm39)	missense	possibly damaging	0.93
R1480:Trrap	UTSW	5	144,755,123 (GRCm39)	missense	probably benign
R1538:Trrap	UTSW	5	144,774,012 (GRCm39)	missense	possibly damaging	0.65
R1751:Trrap	UTSW	5	144,751,385 (GRCm39)	critical splice donor site	probably null
R1779:Trrap	UTSW	5	144,765,400 (GRCm39)	missense	probably benign	0.01
R1792:Trrap	UTSW	5	144,790,396 (GRCm39)	missense	possibly damaging	0.87
R1859:Trrap	UTSW	5	144,767,761 (GRCm39)	missense	probably benign	0.04
R1861:Trrap	UTSW	5	144,752,727 (GRCm39)	splice site	probably null
R1902:Trrap	UTSW	5	144,752,863 (GRCm39)	missense	probably damaging	1.00
R1903:Trrap	UTSW	5	144,752,863 (GRCm39)	missense	probably damaging	1.00
R2021:Trrap	UTSW	5	144,790,298 (GRCm39)	missense	possibly damaging	0.94
R2026:Trrap	UTSW	5	144,739,854 (GRCm39)	missense	possibly damaging	0.86
R2036:Trrap	UTSW	5	144,765,372 (GRCm39)	missense	probably benign	0.08
R2099:Trrap	UTSW	5	144,719,049 (GRCm39)	missense	possibly damaging	0.46
R2108:Trrap	UTSW	5	144,762,684 (GRCm39)	missense	probably benign	0.01
R2113:Trrap	UTSW	5	144,781,021 (GRCm39)	missense	probably damaging	1.00
R2174:Trrap	UTSW	5	144,758,665 (GRCm39)	missense	probably benign	0.40
R2442:Trrap	UTSW	5	144,754,776 (GRCm39)	missense	probably damaging	1.00
R2568:Trrap	UTSW	5	144,780,179 (GRCm39)	critical splice donor site	probably null
R3442:Trrap	UTSW	5	144,729,062 (GRCm39)	missense	probably benign	0.03
R3853:Trrap	UTSW	5	144,728,975 (GRCm39)	missense	probably damaging	1.00
R4401:Trrap	UTSW	5	144,780,128 (GRCm39)	missense	possibly damaging	0.60
R4493:Trrap	UTSW	5	144,767,858 (GRCm39)	missense	probably benign	0.21
R4524:Trrap	UTSW	5	144,762,131 (GRCm39)	missense	probably benign	0.38
R4569:Trrap	UTSW	5	144,728,928 (GRCm39)	missense	probably benign	0.13
R4672:Trrap	UTSW	5	144,722,290 (GRCm39)	missense	probably damaging	0.97
R4732:Trrap	UTSW	5	144,753,380 (GRCm39)	missense	probably damaging	1.00
R4733:Trrap	UTSW	5	144,753,380 (GRCm39)	missense	probably damaging	1.00
R4791:Trrap	UTSW	5	144,740,087 (GRCm39)	missense	probably damaging	1.00
R4795:Trrap	UTSW	5	144,769,298 (GRCm39)	missense	probably benign	0.06
R4827:Trrap	UTSW	5	144,737,758 (GRCm39)	missense	probably benign	0.02
R4839:Trrap	UTSW	5	144,782,402 (GRCm39)	missense	probably damaging	1.00
R4915:Trrap	UTSW	5	144,742,545 (GRCm39)	missense	probably damaging	0.99
R4951:Trrap	UTSW	5	144,742,530 (GRCm39)	missense	possibly damaging	0.65
R4959:Trrap	UTSW	5	144,793,770 (GRCm39)	missense	probably damaging	1.00
R5049:Trrap	UTSW	5	144,763,527 (GRCm39)	missense	probably damaging	1.00
R5074:Trrap	UTSW	5	144,787,989 (GRCm39)	missense	probably damaging	1.00
R5236:Trrap	UTSW	5	144,754,596 (GRCm39)	missense	probably benign	0.07
R5281:Trrap	UTSW	5	144,750,313 (GRCm39)	missense	probably benign	0.13
R5322:Trrap	UTSW	5	144,781,034 (GRCm39)	missense	probably damaging	1.00
R5457:Trrap	UTSW	5	144,786,787 (GRCm39)	missense	probably damaging	1.00
R5590:Trrap	UTSW	5	144,719,075 (GRCm39)	missense	probably benign	0.05
R5799:Trrap	UTSW	5	144,767,755 (GRCm39)	missense	probably benign
R5885:Trrap	UTSW	5	144,731,603 (GRCm39)	missense	probably damaging	1.00
R5905:Trrap	UTSW	5	144,786,730 (GRCm39)	missense	possibly damaging	0.95
R5908:Trrap	UTSW	5	144,723,518 (GRCm39)	missense	probably damaging	0.96
R5956:Trrap	UTSW	5	144,744,201 (GRCm39)	splice site	silent
R5992:Trrap	UTSW	5	144,746,994 (GRCm39)	missense	probably benign	0.00
R6017:Trrap	UTSW	5	144,781,051 (GRCm39)	missense	probably damaging	1.00
R6029:Trrap	UTSW	5	144,762,724 (GRCm39)	missense	possibly damaging	0.75
R6029:Trrap	UTSW	5	144,754,489 (GRCm39)	missense	possibly damaging	0.94
R6117:Trrap	UTSW	5	144,739,771 (GRCm39)	missense	possibly damaging	0.78
R6166:Trrap	UTSW	5	144,718,791 (GRCm39)	missense	possibly damaging	0.66
R6234:Trrap	UTSW	5	144,776,523 (GRCm39)	splice site	probably null
R6288:Trrap	UTSW	5	144,748,802 (GRCm39)	missense	probably damaging	1.00
R6290:Trrap	UTSW	5	144,741,828 (GRCm39)	missense	probably damaging	1.00
R6316:Trrap	UTSW	5	144,750,336 (GRCm39)	missense	probably benign	0.02
R6398:Trrap	UTSW	5	144,727,680 (GRCm39)	missense	possibly damaging	0.83
R6413:Trrap	UTSW	5	144,720,856 (GRCm39)	missense	possibly damaging	0.83
R6499:Trrap	UTSW	5	144,793,812 (GRCm39)	missense	probably damaging	1.00
R6529:Trrap	UTSW	5	144,771,014 (GRCm39)	missense	probably benign	0.06
R6574:Trrap	UTSW	5	144,752,360 (GRCm39)	critical splice donor site	probably null
R6631:Trrap	UTSW	5	144,708,460 (GRCm39)	missense	possibly damaging	0.94
R6727:Trrap	UTSW	5	144,793,760 (GRCm39)	missense	probably damaging	1.00
R6776:Trrap	UTSW	5	144,788,066 (GRCm39)	nonsense	probably null
R6914:Trrap	UTSW	5	144,720,853 (GRCm39)	missense	possibly damaging	0.83
R6942:Trrap	UTSW	5	144,720,853 (GRCm39)	missense	possibly damaging	0.83
R6945:Trrap	UTSW	5	144,727,665 (GRCm39)	missense	possibly damaging	0.66
R7023:Trrap	UTSW	5	144,728,964 (GRCm39)	missense	possibly damaging	0.64
R7107:Trrap	UTSW	5	144,733,945 (GRCm39)	missense	probably benign	0.05
R7139:Trrap	UTSW	5	144,739,988 (GRCm39)	missense	possibly damaging	0.65
R7148:Trrap	UTSW	5	144,758,613 (GRCm39)	missense	possibly damaging	0.77
R7167:Trrap	UTSW	5	144,776,424 (GRCm39)	missense	probably benign	0.39
R7171:Trrap	UTSW	5	144,730,859 (GRCm39)	missense	probably damaging	1.00
R7205:Trrap	UTSW	5	144,779,517 (GRCm39)	missense	possibly damaging	0.94
R7215:Trrap	UTSW	5	144,733,945 (GRCm39)	missense	probably benign	0.05
R7255:Trrap	UTSW	5	144,795,764 (GRCm39)	missense	probably damaging	1.00
R7261:Trrap	UTSW	5	144,782,287 (GRCm39)	missense	possibly damaging	0.67
R7264:Trrap	UTSW	5	144,751,333 (GRCm39)	missense	probably benign	0.05
R7372:Trrap	UTSW	5	144,726,208 (GRCm39)	missense	probably benign
R7447:Trrap	UTSW	5	144,776,284 (GRCm39)	missense	probably damaging	0.97
R7449:Trrap	UTSW	5	144,788,019 (GRCm39)	missense	probably damaging	1.00
R7655:Trrap	UTSW	5	144,779,422 (GRCm39)	missense	probably damaging	1.00
R7656:Trrap	UTSW	5	144,779,422 (GRCm39)	missense	probably damaging	1.00
R7662:Trrap	UTSW	5	144,769,321 (GRCm39)	missense	probably benign	0.00
R7716:Trrap	UTSW	5	144,713,956 (GRCm39)	missense	possibly damaging	0.73
R8143:Trrap	UTSW	5	144,772,707 (GRCm39)	splice site	probably null
R8183:Trrap	UTSW	5	144,765,343 (GRCm39)	missense	probably benign	0.01
R8265:Trrap	UTSW	5	144,722,344 (GRCm39)	missense	possibly damaging	0.53
R8273:Trrap	UTSW	5	144,727,975 (GRCm39)	missense	probably damaging	1.00
R8556:Trrap	UTSW	5	144,762,747 (GRCm39)	missense	probably benign	0.44
R8674:Trrap	UTSW	5	144,727,842 (GRCm39)	missense	probably benign	0.02
R8777:Trrap	UTSW	5	144,773,949 (GRCm39)	missense	probably benign	0.10
R8777-TAIL:Trrap	UTSW	5	144,773,949 (GRCm39)	missense	probably benign	0.10
R8817:Trrap	UTSW	5	144,782,348 (GRCm39)	missense	probably damaging	1.00
R8841:Trrap	UTSW	5	144,781,021 (GRCm39)	missense	probably damaging	1.00
R8871:Trrap	UTSW	5	144,758,649 (GRCm39)	missense	probably benign	0.30
R8937:Trrap	UTSW	5	144,757,063 (GRCm39)	missense	probably damaging	1.00
R8966:Trrap	UTSW	5	144,740,162 (GRCm39)	missense	probably damaging	0.96
R9010:Trrap	UTSW	5	144,783,226 (GRCm39)	missense	probably damaging	1.00
R9095:Trrap	UTSW	5	144,733,961 (GRCm39)	missense	probably damaging	1.00
R9127:Trrap	UTSW	5	144,767,830 (GRCm39)	missense	probably benign	0.16
R9132:Trrap	UTSW	5	144,726,362 (GRCm39)	missense	probably benign	0.03
R9224:Trrap	UTSW	5	144,708,049 (GRCm39)	missense	possibly damaging	0.70
R9338:Trrap	UTSW	5	144,727,925 (GRCm39)	missense	probably benign
R9380:Trrap	UTSW	5	144,769,981 (GRCm39)	missense	probably benign
R9404:Trrap	UTSW	5	144,752,225 (GRCm39)	missense	possibly damaging	0.85
R9457:Trrap	UTSW	5	144,763,478 (GRCm39)	missense	probably damaging	1.00
R9464:Trrap	UTSW	5	144,763,517 (GRCm39)	missense	probably damaging	0.99
R9504:Trrap	UTSW	5	144,742,904 (GRCm39)	missense	probably damaging	1.00
R9583:Trrap	UTSW	5	144,777,330 (GRCm39)	missense	probably damaging	1.00
R9584:Trrap	UTSW	5	144,777,330 (GRCm39)	missense	probably damaging	1.00
R9585:Trrap	UTSW	5	144,777,330 (GRCm39)	missense	probably damaging	1.00
R9608:Trrap	UTSW	5	144,780,128 (GRCm39)	missense	possibly damaging	0.60
R9728:Trrap	UTSW	5	144,726,193 (GRCm39)	missense	probably benign	0.22
R9782:Trrap	UTSW	5	144,758,716 (GRCm39)	missense	probably damaging	0.99
X0060:Trrap	UTSW	5	144,780,171 (GRCm39)	missense	probably damaging	0.96
Z1088:Trrap	UTSW	5	144,771,007 (GRCm39)	missense	probably benign	0.00
Z1177:Trrap	UTSW	5	144,756,518 (GRCm39)	missense	probably damaging	1.00
Z1177:Trrap	UTSW	5	144,747,154 (GRCm39)	missense
Z1177:Trrap	UTSW	5	144,793,761 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTTGAAAAGGTACTGGTCCTGCAC -3'
(R):5'- AGGTTTTCCCATGACCGCTGTC -3'

Sequencing Primer
(F):5'- GTTAGGTCACAGTATCATACCAAGC -3'
(R):5'- ATGACCGCTGTCCAGTGAG -3'

Posted On

2014-05-23