Mutagenetix > Incidental Mutation

Incidental Mutation 'R5942:Zfp352'

460335

Institutional Source

Beutler Lab

Gene Symbol

Zfp352

Ensembl Gene

ENSMUSG00000070902

Gene Name

zinc finger protein 352

Synonyms

2czf48

MMRRC Submission

044134-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5942 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

90218820-90225702 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 90225070 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Asparagine at position 482 (K482N)

Ref Sequence

ENSEMBL: ENSMUSP00000102746 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080541] [ENSMUST00000107129]

AlphaFold

A2AML7

Predicted Effect

probably damaging
Transcript: ENSMUST00000080541
AA Change: K482N

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: K482N

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000107129
AA Change: K482N

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: K482N

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Coding Region Coverage

1x: 99.8%
3x: 99.3%
10x: 96.7%
20x: 89.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	C	T	17: 46,312,407	V860M	probably benign	Het
Accs	A	T	2: 93,836,047	L432M	probably damaging	Het
Actrt3	T	A	3: 30,598,664	N94Y	possibly damaging	Het
Adamts18	T	C	8: 113,777,748	Q80R	probably benign	Het
Ccr8	G	T	9: 120,094,706	V296F	probably damaging	Het
Cep170	T	A	1: 176,756,419	E798V	probably damaging	Het
Cttnbp2	T	A	6: 18,448,440	E73D	probably damaging	Het
Cyp2a4	A	T	7: 26,310,704		probably null	Het
Dync2h1	A	T	9: 7,117,466	Y41*	probably null	Het
Enc1	G	A	13: 97,246,379	D466N	probably benign	Het
Enpp1	C	A	10: 24,676,068	E138*	probably null	Het
Ezh2	T	C	6: 47,577,582	R27G	possibly damaging	Het
Fam189a2	A	G	19: 23,986,470	V245A	probably damaging	Het
Fut10	A	G	8: 31,201,457	N110S	possibly damaging	Het
Glt1d1	A	T	5: 127,644,470		probably null	Het
Gm14393	G	A	2: 175,061,896	Q73*	probably null	Het
Gm6904	G	A	14: 59,260,144	P13S	probably benign	Het
Gpr156	C	T	16: 38,004,902	P494S	probably benign	Het
Has2	T	A	15: 56,667,796	K508*	probably null	Het
Hc	A	T	2: 35,028,125	C715*	probably null	Het
Hook2	C	T	8: 84,994,780		probably null	Het
Klhdc7b	A	C	15: 89,387,431	I839L	probably benign	Het
Kndc1	T	C	7: 139,936,879	L1584P	probably damaging	Het
Lamp1	T	C	8: 13,173,941	F358L	probably damaging	Het
Man2a1	A	G	17: 64,625,380	K154R	probably benign	Het
Mga	A	G	2: 119,946,959	I1871V	probably benign	Het
Mgmt	T	A	7: 137,121,490	D96E	probably benign	Het
Morc2a	C	A	11: 3,679,936	T424K	probably damaging	Het
Myo1g	A	G	11: 6,514,888	L462P	probably damaging	Het
Ncaph	C	A	2: 127,116,688		probably null	Het
Nlrc3	A	T	16: 3,949,429	D969E	probably damaging	Het
Nt5c3	A	T	6: 56,897,854		probably null	Het
Olfr1098	T	C	2: 86,923,406	N42S	probably damaging	Het
Olfr1170	A	C	2: 88,224,572	I153M	probably benign	Het
Olfr1255	G	T	2: 89,816,340	E5*	probably null	Het
Olfr194	A	T	16: 59,119,676	Y131*	probably null	Het
Parp14	C	A	16: 35,839,367	M1628I	probably damaging	Het
Parp8	G	T	13: 116,869,433	P693Q	probably benign	Het
Parp9	A	G	16: 35,971,889	D485G	possibly damaging	Het
Pcdha1	T	A	18: 36,930,391	V36D	probably damaging	Het
Pcdhb5	C	T	18: 37,320,785	Q73*	probably null	Het
Pecam1	G	T	11: 106,661,983		probably benign	Het
Pex1	A	G	5: 3,610,277	I527V	probably benign	Het
Ppp4r4	T	A	12: 103,587,447	V388D	possibly damaging	Het
Psmc4	C	T	7: 28,047,055	V202I	probably damaging	Het
Ptx3	T	G	3: 66,220,063	M1R	probably null	Het
Rimbp3	A	G	16: 17,211,888	T1059A	probably benign	Het
Rmdn3	G	A	2: 119,147,577	A181V	probably damaging	Het
Rnase10	A	T	14: 51,009,278	M38L	probably benign	Het
Sec16b	T	A	1: 157,531,350	Y118N	probably damaging	Het
Sigmar1	T	C	4: 41,741,159	T32A	probably benign	Het
Srcap	A	G	7: 127,538,008	D954G	probably damaging	Het
Tfrc	A	G	16: 32,626,715	N618S	possibly damaging	Het
Tnrc6a	A	G	7: 123,186,665	D1028G	probably damaging	Het
Tns3	A	T	11: 8,435,860	D1379E	probably damaging	Het
Ttn	A	T	2: 76,750,161	C23463S	possibly damaging	Het
Ufl1	T	C	4: 25,250,619	T745A	probably benign	Het
Vmn1r231	T	A	17: 20,890,155	Y166F	possibly damaging	Het
Wbp1l	C	A	19: 46,654,430	T290K	probably damaging	Het
Wdr25	A	G	12: 108,898,466	N179S	probably benign	Het
Wdr62	G	A	7: 30,243,079	Q1035*	probably null	Het
Yif1a	C	T	19: 5,091,641	R196C	probably damaging	Het
Zfp647	G	A	15: 76,912,085	P125L	probably damaging	Het

Other mutations in Zfp352

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01963:Zfp352	APN	4	90224154	missense	possibly damaging	0.95
IGL02252:Zfp352	APN	4	90224130	missense	probably benign	0.02
IGL03156:Zfp352	APN	4	90224087	missense	possibly damaging	0.57
IGL03167:Zfp352	APN	4	90224702	missense	probably damaging	0.99
IGL03190:Zfp352	APN	4	90223757	missense	possibly damaging	0.94
IGL03335:Zfp352	APN	4	90224346	missense	probably damaging	0.99
R0051:Zfp352	UTSW	4	90224285	missense	probably damaging	0.99
R0403:Zfp352	UTSW	4	90225009	missense	possibly damaging	0.60
R0550:Zfp352	UTSW	4	90224690	missense	probably damaging	0.99
R0671:Zfp352	UTSW	4	90223919	missense	probably benign
R1034:Zfp352	UTSW	4	90224156	missense	possibly damaging	0.94
R1754:Zfp352	UTSW	4	90223809	missense	probably benign	0.23
R2016:Zfp352	UTSW	4	90225171	missense	probably benign	0.42
R2064:Zfp352	UTSW	4	90225120	missense	probably benign	0.08
R2308:Zfp352	UTSW	4	90225243	missense	probably benign	0.00
R3552:Zfp352	UTSW	4	90225102	missense	probably benign	0.33
R3794:Zfp352	UTSW	4	90225149	missense	probably damaging	1.00
R3795:Zfp352	UTSW	4	90225149	missense	probably damaging	1.00
R4135:Zfp352	UTSW	4	90225024	missense	probably damaging	0.96
R4356:Zfp352	UTSW	4	90223834	missense	possibly damaging	0.91
R4409:Zfp352	UTSW	4	90225164	missense	probably benign	0.00
R4590:Zfp352	UTSW	4	90224535	missense	probably damaging	0.98
R4614:Zfp352	UTSW	4	90225081	missense	probably benign	0.00
R4617:Zfp352	UTSW	4	90225081	missense	probably benign	0.00
R4618:Zfp352	UTSW	4	90225081	missense	probably benign	0.00
R4741:Zfp352	UTSW	4	90224940	missense	possibly damaging	0.94
R4931:Zfp352	UTSW	4	90224304	missense	probably damaging	0.98
R4959:Zfp352	UTSW	4	90224139	missense	probably benign	0.01
R4973:Zfp352	UTSW	4	90224139	missense	probably benign	0.01
R5167:Zfp352	UTSW	4	90224216	missense	possibly damaging	0.94
R5260:Zfp352	UTSW	4	90224460	missense	probably damaging	0.99
R5524:Zfp352	UTSW	4	90225104	missense	possibly damaging	0.95
R6802:Zfp352	UTSW	4	90225200	missense	probably benign	0.33
R6819:Zfp352	UTSW	4	90224699	missense	probably benign
R7072:Zfp352	UTSW	4	90224424	missense	probably benign	0.00
R7099:Zfp352	UTSW	4	90224880	missense	probably benign	0.00
R7569:Zfp352	UTSW	4	90223659	missense	possibly damaging	0.77
R7645:Zfp352	UTSW	4	90224777	missense	probably benign	0.13
R7705:Zfp352	UTSW	4	90225275	missense	possibly damaging	0.94
R8424:Zfp352	UTSW	4	90224243	missense	possibly damaging	0.87
R9180:Zfp352	UTSW	4	90224881	missense	probably benign	0.38
R9378:Zfp352	UTSW	4	90224338	missense	probably benign	0.13
R9509:Zfp352	UTSW	4	90224706	missense	probably damaging	0.99
R9623:Zfp352	UTSW	4	90224891	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGGGTGAATCCTCATACCCTTC -3'
(R):5'- AGCATGTTTCTTCTCCAGATGC -3'

Sequencing Primer
(F):5'- TCAGAAAAACTCTTCGGCTTCCAG -3'
(R):5'- CCAGATGCTTTTTGAGATAAAGCAG -3'

Posted On

2017-02-28