Mutagenetix > Incidental Mutation

Incidental Mutation 'R7569:Zfp352'

585626

Institutional Source

Beutler Lab

Gene Symbol

Zfp352

Ensembl Gene

ENSMUSG00000070902

Gene Name

zinc finger protein 352

Synonyms

2czf48

MMRRC Submission

045657-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7569 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

90218820-90225702 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 90223659 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 12 (P12L)

Ref Sequence

ENSEMBL: ENSMUSP00000102746 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080541] [ENSMUST00000107129]

AlphaFold

A2AML7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000080541
AA Change: P12L

PolyPhen 2 Score 0.775 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: P12L

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107129
AA Change: P12L

PolyPhen 2 Score 0.775 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: P12L

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Meta Mutation Damage Score

0.1415

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (58/59)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700007K13Rik	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	2: 28,466,110 (GRCm38)		probably benign	Het
9330182O14Rik	A	T	15: 40,144,948 (GRCm38)	T73S	unknown	Het
Acvrl1	A	G	15: 101,135,755 (GRCm38)	Q106R	probably benign	Het
Adgb	A	T	10: 10,431,252 (GRCm38)	D329E	probably benign	Het
Ankrd12	A	T	17: 65,982,905 (GRCm38)	D1844E	probably damaging	Het
Ankub1	G	A	3: 57,665,618 (GRCm38)	R228*	probably null	Het
Apol6	A	C	15: 77,050,698 (GRCm38)		probably benign	Het
Ascc2	G	A	11: 4,679,506 (GRCm38)	V618M	probably damaging	Het
Asic3	A	G	5: 24,414,048 (GRCm38)	T113A	probably benign	Het
BC034090	T	A	1: 155,217,405 (GRCm38)	H769L	probably benign	Het
Bcl11a	G	T	11: 24,085,458 (GRCm38)	E65*	probably null	Het
Birc6	G	T	17: 74,598,082 (GRCm38)	R1290L	possibly damaging	Het
C6	A	G	15: 4,789,581 (GRCm38)	E465G	probably benign	Het
Cav2	T	A	6: 17,282,079 (GRCm38)	I112N	probably damaging	Het
Cep131	G	A	11: 120,066,713 (GRCm38)	A848V	probably damaging	Het
Col8a1	T	A	16: 57,627,192 (GRCm38)	I652F	unknown	Het
Cul4a	A	G	8: 13,123,493 (GRCm38)	N180S	probably benign	Het
Dhx38	A	G	8: 109,560,695 (GRCm38)	S214P	probably damaging	Het
Dmxl2	A	G	9: 54,415,987 (GRCm38)	V1197A	possibly damaging	Het
Dusp27	T	C	1: 166,108,035 (GRCm38)	D198G	probably damaging	Het
Dynlt1f	A	G	17: 6,655,782 (GRCm38)	S7P	not run	Het
Epha1	T	A	6: 42,365,422 (GRCm38)	T331S	possibly damaging	Het
Gm10436	A	T	12: 88,176,315 (GRCm38)	Y373N	probably benign	Het
Gm49333	T	A	16: 20,642,487 (GRCm38)	Y641*	probably null	Het
Hus1b	A	G	13: 30,946,864 (GRCm38)	Y271H	probably damaging	Het
Kmt2b	A	G	7: 30,569,553 (GRCm38)	V2610A	possibly damaging	Het
Lama2	A	G	10: 27,265,050 (GRCm38)	L651P	probably damaging	Het
Lgr5	T	C	10: 115,462,756 (GRCm38)	Y361C	probably damaging	Het
Map1s	T	C	8: 70,913,498 (GRCm38)	V349A	probably benign	Het
Map3k6	A	G	4: 133,250,077 (GRCm38)	R912G	probably benign	Het
Marc2	A	T	1: 184,841,425 (GRCm38)	F92Y	possibly damaging	Het
Nampt	T	A	12: 32,850,434 (GRCm38)	H459Q	probably benign	Het
Nlrp1a	T	C	11: 71,109,043 (GRCm38)	M817V	probably benign	Het
Npsr1	A	G	9: 24,313,730 (GRCm38)	R345G	probably benign	Het
Nuak2	G	A	1: 132,316,281 (GRCm38)	A18T	possibly damaging	Het
Olfr1428	T	C	19: 12,109,021 (GRCm38)	D175G	possibly damaging	Het
Olfr993	A	G	2: 85,414,135 (GRCm38)	V248A	probably damaging	Het
Ppp4r3b	T	C	11: 29,188,540 (GRCm38)	F296S	possibly damaging	Het
Pycr2	T	C	1: 180,904,518 (GRCm38)	F19L	probably benign	Het
Robo2	T	A	16: 74,035,115 (GRCm38)	T226S	possibly damaging	Het
Rock1	A	T	18: 10,140,194 (GRCm38)	N132K	probably damaging	Het
Rp1	A	T	1: 4,284,840 (GRCm38)	L379Q	unknown	Het
Sema6d	A	G	2: 124,657,972 (GRCm38)	I323V	possibly damaging	Het
Slc12a4	A	G	8: 105,945,847 (GRCm38)	I814T	probably damaging	Het
Slc18a2	G	A	19: 59,284,152 (GRCm38)	G352R	probably damaging	Het
Slc39a14	T	C	14: 70,309,827 (GRCm38)	T357A	possibly damaging	Het
Smarcad1	T	A	6: 65,052,711 (GRCm38)	D94E	probably benign	Het
Sorcs2	A	G	5: 36,025,876 (GRCm38)	Y1018H	probably benign	Het
Srebf1	G	T	11: 60,200,121 (GRCm38)	T1069K	possibly damaging	Het
St3gal3	A	G	4: 117,964,356 (GRCm38)	V123A	probably benign	Het
Stradb	A	G	1: 58,991,151 (GRCm38)	Y188C	unknown	Het
Sult2a2	T	C	7: 13,779,505 (GRCm38)	F186L	probably benign	Het
Syne2	A	G	12: 75,927,390 (GRCm38)	T1120A	probably benign	Het
Taok1	A	T	11: 77,555,614 (GRCm38)	S430T	probably benign	Het
Tmem62	A	T	2: 121,006,930 (GRCm38)	I573L	probably benign	Het
Trav9-1	T	C	14: 53,488,124 (GRCm38)	S7P	probably benign	Het
U2af1l4	A	T	7: 30,563,557 (GRCm38)	I24F	probably damaging	Het
Usp33	T	A	3: 152,391,665 (GRCm38)	I840N	probably damaging	Het
Vmn2r74	A	C	7: 85,952,336 (GRCm38)	I698S	probably damaging	Het
Zfp507	T	A	7: 35,794,544 (GRCm38)	E358V	probably damaging	Het

Other mutations in Zfp352

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01963:Zfp352	APN	4	90,224,154 (GRCm38)	missense	possibly damaging	0.95
IGL02252:Zfp352	APN	4	90,224,130 (GRCm38)	missense	probably benign	0.02
IGL03156:Zfp352	APN	4	90,224,087 (GRCm38)	missense	possibly damaging	0.57
IGL03167:Zfp352	APN	4	90,224,702 (GRCm38)	missense	probably damaging	0.99
IGL03190:Zfp352	APN	4	90,223,757 (GRCm38)	missense	possibly damaging	0.94
IGL03335:Zfp352	APN	4	90,224,346 (GRCm38)	missense	probably damaging	0.99
R0051:Zfp352	UTSW	4	90,224,285 (GRCm38)	missense	probably damaging	0.99
R0403:Zfp352	UTSW	4	90,225,009 (GRCm38)	missense	possibly damaging	0.60
R0550:Zfp352	UTSW	4	90,224,690 (GRCm38)	missense	probably damaging	0.99
R0671:Zfp352	UTSW	4	90,223,919 (GRCm38)	missense	probably benign
R1034:Zfp352	UTSW	4	90,224,156 (GRCm38)	missense	possibly damaging	0.94
R1754:Zfp352	UTSW	4	90,223,809 (GRCm38)	missense	probably benign	0.23
R2016:Zfp352	UTSW	4	90,225,171 (GRCm38)	missense	probably benign	0.42
R2064:Zfp352	UTSW	4	90,225,120 (GRCm38)	missense	probably benign	0.08
R2308:Zfp352	UTSW	4	90,225,243 (GRCm38)	missense	probably benign	0.00
R3552:Zfp352	UTSW	4	90,225,102 (GRCm38)	missense	probably benign	0.33
R3794:Zfp352	UTSW	4	90,225,149 (GRCm38)	missense	probably damaging	1.00
R3795:Zfp352	UTSW	4	90,225,149 (GRCm38)	missense	probably damaging	1.00
R4135:Zfp352	UTSW	4	90,225,024 (GRCm38)	missense	probably damaging	0.96
R4356:Zfp352	UTSW	4	90,223,834 (GRCm38)	missense	possibly damaging	0.91
R4409:Zfp352	UTSW	4	90,225,164 (GRCm38)	missense	probably benign	0.00
R4590:Zfp352	UTSW	4	90,224,535 (GRCm38)	missense	probably damaging	0.98
R4614:Zfp352	UTSW	4	90,225,081 (GRCm38)	missense	probably benign	0.00
R4617:Zfp352	UTSW	4	90,225,081 (GRCm38)	missense	probably benign	0.00
R4618:Zfp352	UTSW	4	90,225,081 (GRCm38)	missense	probably benign	0.00
R4741:Zfp352	UTSW	4	90,224,940 (GRCm38)	missense	possibly damaging	0.94
R4931:Zfp352	UTSW	4	90,224,304 (GRCm38)	missense	probably damaging	0.98
R4959:Zfp352	UTSW	4	90,224,139 (GRCm38)	missense	probably benign	0.01
R4973:Zfp352	UTSW	4	90,224,139 (GRCm38)	missense	probably benign	0.01
R5167:Zfp352	UTSW	4	90,224,216 (GRCm38)	missense	possibly damaging	0.94
R5260:Zfp352	UTSW	4	90,224,460 (GRCm38)	missense	probably damaging	0.99
R5524:Zfp352	UTSW	4	90,225,104 (GRCm38)	missense	possibly damaging	0.95
R5942:Zfp352	UTSW	4	90,225,070 (GRCm38)	missense	probably damaging	0.98
R6802:Zfp352	UTSW	4	90,225,200 (GRCm38)	missense	probably benign	0.33
R6819:Zfp352	UTSW	4	90,224,699 (GRCm38)	missense	probably benign
R7072:Zfp352	UTSW	4	90,224,424 (GRCm38)	missense	probably benign	0.00
R7099:Zfp352	UTSW	4	90,224,880 (GRCm38)	missense	probably benign	0.00
R7645:Zfp352	UTSW	4	90,224,777 (GRCm38)	missense	probably benign	0.13
R7705:Zfp352	UTSW	4	90,225,275 (GRCm38)	missense	possibly damaging	0.94
R8424:Zfp352	UTSW	4	90,224,243 (GRCm38)	missense	possibly damaging	0.87
R9180:Zfp352	UTSW	4	90,224,881 (GRCm38)	missense	probably benign	0.38
R9378:Zfp352	UTSW	4	90,224,338 (GRCm38)	missense	probably benign	0.13
R9509:Zfp352	UTSW	4	90,224,706 (GRCm38)	missense	probably damaging	0.99
R9623:Zfp352	UTSW	4	90,224,891 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGTATGATGCCCTGACCTCC -3'
(R):5'- TGGAAACCAATGGCTTTTCCTG -3'

Sequencing Primer
(F):5'- GACCTCCAGCACATATTCTCTG -3'
(R):5'- AAACCAATGGCTTTTCCTGGGTATG -3'

Posted On

2019-10-17