|Institutional Source||Beutler Lab|
|Gene Name||carboxylesterase 4A|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R6876 (G1)|
|Chromosomal Location||105131800-105150109 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to A at 105144992 bp|
|Amino Acid Change||Valine to Aspartic acid at position 258 (V258D)|
|Ref Sequence||ENSEMBL: ENSMUSP00000125062 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000161289]|
|Predicted Effect||possibly damaging
AA Change: V258D
PolyPhen 2 Score 0.561 (Sensitivity: 0.88; Specificity: 0.91)
AA Change: V258D
|Coding Region Coverage||
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They also participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene, also called CES6, encodes a secreted enzyme, and may play a role in the detoxification of drugs and xenobiotics in neural and other tissues of the body and in the cerebrospinal fluid. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq, Jun 2010]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ces4a||
(F):5'- AAAGGTGCTGGCTTTAAATCTAGG -3'
(R):5'- ACCTCAGGCATTCCACCATG -3'
(F):5'- GCTGGCTTTAAATCTAGGCTCTAGC -3'
(R):5'- AGGCATTCCACCATGACCTTCG -3'