Incidental Mutation 'PIT4486001:Mkrn2os'
ID 556100
Institutional Source Beutler Lab
Gene Symbol Mkrn2os
Ensembl Gene ENSMUSG00000068011
Gene Name makorin, ring finger protein 2, opposite strand
Synonyms 2510049J12Rik
Accession Numbers
Essential gene? Probably non essential (E-score: 0.071) question?
Stock # PIT4486001 (G1)
Quality Score 143.008
Status Not validated
Chromosome 6
Chromosomal Location 115560508-115569537 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 115562444 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 173 (D173G)
Ref Sequence ENSEMBL: ENSMUSP00000108580 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000088962] [ENSMUST00000112957]
AlphaFold D3Z393
Predicted Effect probably benign
Transcript: ENSMUST00000088962
Predicted Effect probably benign
Transcript: ENSMUST00000112957
AA Change: D173G

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)
Coding Region Coverage
  • 1x: 93.0%
  • 3x: 90.5%
  • 10x: 84.1%
  • 20x: 70.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 51 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700018F24Rik T C 5: 144,980,914 (GRCm39) S108P probably damaging Het
Abhd16b A T 2: 181,135,752 (GRCm39) Q218L probably benign Het
Abhd3 T A 18: 10,645,233 (GRCm39) I354F probably benign Het
Abt1 T C 13: 23,607,851 (GRCm39) Y51C possibly damaging Het
Actl9 T A 17: 33,653,172 (GRCm39) Y411N possibly damaging Het
Ano4 A G 10: 88,828,891 (GRCm39) V516A probably damaging Het
Bptf A T 11: 106,945,614 (GRCm39) S2542T probably damaging Het
Card11 C T 5: 140,862,163 (GRCm39) V1045M probably damaging Het
Ccdc121 T A 5: 31,645,087 (GRCm39) I280K probably damaging Het
Cd300ld2 CGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGTCAGAACTGTGGATGGCACAACTGTGCATGGCAGAACTGTGGATGGCACAACTGTGGATGGCAGAACTGTGG CGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGGCAGAACTGTGGATGTCAGAACTGTGGATGTCAGAACTGTGGATGGCACAACTGTGCATGGCAGAACTGTGGATGGCACAACTGTGGATGGCAGAACTGTGG 11: 114,903,257 (GRCm39) probably benign Het
Cdh3 A G 8: 107,268,122 (GRCm39) K386E possibly damaging Het
Cks1b C A 3: 89,323,621 (GRCm39) Q49H probably damaging Het
Clpb A T 7: 101,313,139 (GRCm39) D41V probably benign Het
Cyp3a11 A T 5: 145,797,302 (GRCm39) M359K probably damaging Het
Cyp3a13 A T 5: 137,908,228 (GRCm39) I207N probably benign Het
Dennd4c T A 4: 86,717,701 (GRCm39) L566* probably null Het
Dhtkd1 A T 2: 5,904,806 (GRCm39) D859E probably benign Het
Efcab6 T C 15: 83,857,514 (GRCm39) D295G probably benign Het
Fcgbp A G 7: 27,774,698 (GRCm39) T91A possibly damaging Het
Gm11569 GCAGCTGGGCCTGCAGCAGCTGGAAATGCAGCAGCTAGGACGGCAACA GCA 11: 99,689,491 (GRCm39) probably benign Het
Gsdma3 A G 11: 98,528,880 (GRCm39) K454E unknown Het
Herc1 T A 9: 66,279,671 (GRCm39) I193N probably damaging Het
Kdm5b T A 1: 134,556,423 (GRCm39) L1370Q probably damaging Het
Lrrc37 A T 11: 103,509,027 (GRCm39) H980Q unknown Het
Map4 T G 9: 109,901,682 (GRCm39) V965G probably damaging Het
Ndfip2 A G 14: 105,532,300 (GRCm39) D232G probably damaging Het
Nipal2 C T 15: 34,584,875 (GRCm39) G231D probably damaging Het
Notch3 A T 17: 32,373,737 (GRCm39) N490K probably damaging Het
Or5p68 A G 7: 107,945,529 (GRCm39) S220P possibly damaging Het
Or8g50 T A 9: 39,648,535 (GRCm39) C141* probably null Het
Prkar2a T C 9: 108,610,326 (GRCm39) L185S probably damaging Het
Ptpn9 T G 9: 56,968,287 (GRCm39) N542K probably damaging Het
Pus10 G A 11: 23,662,326 (GRCm39) probably null Het
Pyroxd2 A G 19: 42,728,828 (GRCm39) S191P probably benign Het
Rab15 T A 12: 76,848,716 (GRCm39) K122* probably null Het
Rara A G 11: 98,864,321 (GRCm39) N416S possibly damaging Het
Rims2 T C 15: 39,339,916 (GRCm39) V870A possibly damaging Het
Sec16a T C 2: 26,315,785 (GRCm39) T293A Het
Slc26a3 G A 12: 31,520,949 (GRCm39) D718N probably benign Het
Slc44a5 G A 3: 153,964,659 (GRCm39) V520I possibly damaging Het
Spata31e2 G A 1: 26,724,410 (GRCm39) P257S probably damaging Het
Tgfb2 A T 1: 186,422,924 (GRCm39) Y142N probably benign Het
Tgfbi A T 13: 56,777,607 (GRCm39) I364F probably damaging Het
Tmem144 A C 3: 79,734,174 (GRCm39) D176E probably benign Het
Tns4 A T 11: 98,962,161 (GRCm39) L612Q probably damaging Het
Toe1 A G 4: 116,663,692 (GRCm39) L76S probably damaging Het
Trank1 T C 9: 111,219,175 (GRCm39) F1971L probably damaging Het
Tsen54 G T 11: 115,713,422 (GRCm39) V481F probably damaging Het
Uimc1 A G 13: 55,223,381 (GRCm39) L297P probably damaging Het
Wnt8a T C 18: 34,680,636 (GRCm39) Y334H probably damaging Het
Zfp281 A G 1: 136,554,741 (GRCm39) D573G possibly damaging Het
Other mutations in Mkrn2os
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01759:Mkrn2os APN 6 115,569,292 (GRCm39) missense probably benign 0.37
IGL01982:Mkrn2os APN 6 115,562,492 (GRCm39) missense probably damaging 1.00
IGL02883:Mkrn2os APN 6 115,563,670 (GRCm39) nonsense probably null
R0458:Mkrn2os UTSW 6 115,563,631 (GRCm39) missense probably damaging 0.99
R1989:Mkrn2os UTSW 6 115,566,311 (GRCm39) missense probably damaging 0.99
R7058:Mkrn2os UTSW 6 115,563,635 (GRCm39) missense probably benign 0.01
R7167:Mkrn2os UTSW 6 115,562,474 (GRCm39) missense probably damaging 0.99
R7609:Mkrn2os UTSW 6 115,563,687 (GRCm39) missense possibly damaging 0.86
R8958:Mkrn2os UTSW 6 115,562,317 (GRCm39) missense probably benign 0.18
R8959:Mkrn2os UTSW 6 115,562,317 (GRCm39) missense probably benign 0.18
R9051:Mkrn2os UTSW 6 115,562,325 (GRCm39) missense probably benign 0.23
Predicted Primers PCR Primer
(F):5'- CCCTCAGATGCAGCATTTGG -3'
(R):5'- AGCCTTTACTGTTAGCATTCTCAGG -3'

Sequencing Primer
(F):5'- CTCAGATGCAGCATTTGGTCCAG -3'
(R):5'- ATGGTAGCCTCAGGTGTAACC -3'
Posted On 2019-06-07