Mutagenetix > Incidental Mutations

Incidental Mutation 'R7618:Ogfod3'

589011

Institutional Source

Beutler Lab

Gene Symbol

Ogfod3

Ensembl Gene

ENSMUSG00000025169

Gene Name

2-oxoglutarate and iron-dependent oxygenase domain containing 3

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R7618 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

121177591-121204711 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 121202978 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Phenylalanine at position 69 (V69F)

Ref Sequence

ENSEMBL: ENSMUSP00000026169 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026169] [ENSMUST00000038831] [ENSMUST00000106117] [ENSMUST00000124768]

AlphaFold

Q9D136

Predicted Effect

probably damaging
Transcript: ENSMUST00000026169
AA Change: V69F

PolyPhen 2 Score 0.965 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000026169
Gene: ENSMUSG00000025169
AA Change: V69F

Domain	Start	End	E-Value	Type
transmembrane domain	39	61	N/A	INTRINSIC
P4Hc	104	302	1.82e-25	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000038831

SMART Domains

Protein: ENSMUSP00000048479
Gene: ENSMUSG00000039307

Domain	Start	End	E-Value	Type
Pfam:Glyco_hydro_20	30	223	2.9e-12	PFAM
low complexity region	528	538	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106117

SMART Domains

Protein: ENSMUSP00000101723
Gene: ENSMUSG00000039307

Domain	Start	End	E-Value	Type
Pfam:Glyco_hydro_20	31	229	1.7e-18	PFAM
low complexity region	455	465	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124768

SMART Domains

Protein: ENSMUSP00000122008
Gene: ENSMUSG00000039307

Domain	Start	End	E-Value	Type
Pfam:Glyco_hydro_20	22	152	8.3e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000151495

SMART Domains

Protein: ENSMUSP00000123073
Gene: ENSMUSG00000039307

Domain	Start	End	E-Value	Type
Pfam:Glyco_hydro_20	27	220	3.1e-12	PFAM
low complexity region	525	535	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.5%

Validation Efficiency

98% (51/52)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610010F05Rik	A	C	11: 23,584,550	C602W	possibly damaging	Het
4930432K21Rik	A	G	8: 84,166,870	Q222R	possibly damaging	Het
Akap11	T	C	14: 78,498,860	D1830G		Het
Alms1	A	G	6: 85,678,417	N2846S	probably benign	Het
Amt	A	C	9: 108,299,878	E228D	probably damaging	Het
Ankar	T	C	1: 72,675,766	M618V	probably benign	Het
Ankrd60	T	C	2: 173,571,041		probably null	Het
Aoc1	A	G	6: 48,906,386	T399A	possibly damaging	Het
Aqp11	T	A	7: 97,737,666	I108F	probably benign	Het
Arfgef3	G	T	10: 18,646,281	Q666K	probably damaging	Het
Bin2	T	A	15: 100,645,013	R430W	probably damaging	Het
Cdh18	T	A	15: 23,366,970	V254D	probably damaging	Het
Cfap46	A	T	7: 139,603,239	S159R		Het
Clnk	A	T	5: 38,736,355	S220T	probably benign	Het
Col19a1	G	T	1: 24,322,084	H608Q	probably benign	Het
Cplx1	C	T	5: 108,525,529	E24K	possibly damaging	Het
Dnah3	A	T	7: 119,978,378	L2031Q	probably damaging	Het
Dok1	T	C	6: 83,032,891	E79G	probably benign	Het
Eif4g3	T	C	4: 138,171,118	S902P	probably damaging	Het
Emilin3	T	A	2: 160,909,279	E183D	probably benign	Het
Fam124b	G	A	1: 80,213,837		probably benign	Het
Gm9195	T	C	14: 72,452,835	Y1816C	probably damaging	Het
Ighv5-9-1	T	A	12: 113,736,199	I98F	probably damaging	Het
Il31ra	G	A	13: 112,551,980	P21L	possibly damaging	Het
Kat6a	A	G	8: 22,862,562	I121V	possibly damaging	Het
Kif11	T	C	19: 37,411,560	W832R	probably benign	Het
Klhl9	T	C	4: 88,720,535	T490A	possibly damaging	Het
Lars	G	T	18: 42,244,891	A153E	probably benign	Het
Muc5b	A	T	7: 141,867,597	I4275L	probably benign	Het
Myo10	T	A	15: 25,726,475	C294*	probably null	Het
Nceh1	T	A	3: 27,183,217		probably null	Het
Ncf1	T	A	5: 134,227,267	T93S	probably benign	Het
Nfatc2	G	A	2: 168,534,999	R545C	probably damaging	Het
Nos1	C	T	5: 117,903,944	P545S	probably benign	Het
Olfr1197	A	T	2: 88,728,836	Y254*	probably null	Het
Phf12	A	G	11: 78,026,134	N272S	unknown	Het
Prkcz	T	A	4: 155,262,482	I581F	probably damaging	Het
Rasgrf2	T	C	13: 91,987,966	H8R		Het
Rb1cc1	T	A	1: 6,265,558		probably null	Het
Rcor2	T	A	19: 7,271,046	M186K	possibly damaging	Het
Rnf111	C	A	9: 70,503,332		probably benign	Het
Serinc3	A	T	2: 163,630,969	F247Y	possibly damaging	Het
Serpina1c	T	A	12: 103,898,770	I206F	probably damaging	Het
Slc25a10	G	A	11: 120,496,971		probably null	Het
Syne2	T	G	12: 75,945,334	H1993Q	probably benign	Het
Tap1	A	G	17: 34,188,238	Y120C	possibly damaging	Het
Tex30	A	T	1: 44,088,250		probably null	Het
Ube2ql1	G	T	13: 69,738,947	Q132K	probably benign	Het
Unc13d	T	C	11: 116,066,721	N803D	probably damaging	Het
Vcan	G	A	13: 89,692,223	S1734F	probably damaging	Het
Wdfy4	T	C	14: 32,985,739	Y2630C		Het
Wdr93	A	G	7: 79,785,726	T668A	probably benign	Het

Other mutations in Ogfod3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01954:Ogfod3	APN	11	121203025	missense	probably benign	0.06
IGL02398:Ogfod3	APN	11	121203025	missense	probably benign	0.06
IGL02458:Ogfod3	APN	11	121200923	missense	probably benign	0.22
IGL03411:Ogfod3	APN	11	121177804	makesense	probably null
PIT4403001:Ogfod3	UTSW	11	121196735	critical splice donor site	probably null
R0145:Ogfod3	UTSW	11	121195070	splice site	probably benign
R1302:Ogfod3	UTSW	11	121183474	missense	probably damaging	1.00
R3926:Ogfod3	UTSW	11	121183429	missense	probably damaging	1.00
R4823:Ogfod3	UTSW	11	121195201	missense	probably benign	0.42
R4825:Ogfod3	UTSW	11	121195201	missense	probably benign	0.42
R4909:Ogfod3	UTSW	11	121197492	missense	probably damaging	1.00
R6378:Ogfod3	UTSW	11	121202935	missense	probably benign	0.01
R6953:Ogfod3	UTSW	11	121202998	missense	probably benign
R7051:Ogfod3	UTSW	11	121195205	missense	probably damaging	0.99
R7741:Ogfod3	UTSW	11	121183536	critical splice acceptor site	probably null
R8112:Ogfod3	UTSW	11	121204550	missense	probably damaging	1.00
R8714:Ogfod3	UTSW	11	121196782	missense	possibly damaging	0.79

Predicted Primers

PCR Primer
(F):5'- GCTGAGTTTCATTTCTGTCACG -3'
(R):5'- TAGTATGATGGCTGCATCCAG -3'

Sequencing Primer
(F):5'- TCTCCTGAAGGTGAAGTTACAGGC -3'
(R):5'- CCAGTGGCTGGTTATCGTCTC -3'

Posted On

2019-10-24