|Institutional Source||Beutler Lab|
|Gene Name||free fatty acid receptor 4|
|Synonyms||Gpr129, O3far1, Pgr4, Gpr120|
|Is this an essential gene?||Probably non essential (E-score: 0.110)|
|Stock #||R8030 (G1)|
|Chromosomal Location||38097079-38114263 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 38107391 bp|
|Amino Acid Change||Isoleucine to Valine at position 193 (I193V)|
|Ref Sequence||ENSEMBL: ENSMUSP00000063660 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000067098]|
|Predicted Effect||possibly damaging
AA Change: I193V
PolyPhen 2 Score 0.796 (Sensitivity: 0.84; Specificity: 0.93)
AA Change: I193V
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
PHENOTYPE: Homozygotes for a null allele show altered taste responses to fatty acids. Homozygotes for another null allele develop obesity, liver steatosis, and impaired glucose metabolism, adipogenesis and lipogenesis on a high-fat diet. Homozygotes for a third allele show altered islet somatostatin secretion. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ffar4||
(F):5'- TGTAAGAGGCAGCACCATGG -3'
(R):5'- TGTTAGGACCCCAAAGTGTC -3'
(F):5'- GCAGCACCATGGGCAAAG -3'
(R):5'- GTTAGGACCCCAAAGTGTCCTCTG -3'