Mutagenetix > Incidental Mutation

Incidental Mutation 'R7966:Vmn2r69'

650370

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r69

Ensembl Gene

ENSMUSG00000091006

Gene Name

vomeronasal 2, receptor 69

Synonyms

MMRRC Submission

046009-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R7966 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85055584-85064884 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 85060762 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 274 (I274T)

Ref Sequence

ENSEMBL: ENSMUSP00000132726 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171213]

AlphaFold

G3XA45

Predicted Effect

possibly damaging
Transcript: ENSMUST00000171213
AA Change: I274T

PolyPhen 2 Score 0.808 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000132726
Gene: ENSMUSG00000091006
AA Change: I274T

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.3e-28	PFAM
Pfam:NCD3G	507	559	1.8e-20	PFAM
Pfam:7tm_3	592	827	3.2e-54	PFAM

Meta Mutation Damage Score

0.1712

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

98% (52/53)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	A	T	12: 71,219,903 (GRCm39)	I817L	probably benign	Het
Adcy8	A	T	15: 64,573,939 (GRCm39)	W1055R	probably damaging	Het
Anks4b	A	G	7: 119,781,923 (GRCm39)	E318G	probably benign	Het
Arid3a	A	G	10: 79,767,889 (GRCm39)	T229A	probably benign	Het
Cckar	T	C	5: 53,858,580 (GRCm39)	K247E	possibly damaging	Het
Cfap52	A	T	11: 67,844,571 (GRCm39)		probably null	Het
Ckap4	A	G	10: 84,363,449 (GRCm39)	V538A	probably damaging	Het
Cped1	T	C	6: 22,059,953 (GRCm39)		probably null	Het
Ctsf	T	G	19: 4,906,567 (GRCm39)	F165V	probably damaging	Het
Cyp4v3	G	T	8: 45,785,954 (GRCm39)	A21E	probably benign	Het
Cytip	T	C	2: 58,037,944 (GRCm39)	E140G	probably damaging	Het
D130043K22Rik	A	C	13: 25,077,406 (GRCm39)	Q1013P	probably damaging	Het
Dpp6	A	G	5: 27,928,370 (GRCm39)	M763V	probably benign	Het
Eps15	T	A	4: 109,178,340 (GRCm39)	Y193N	probably damaging	Het
Ghrhr	T	C	6: 55,356,083 (GRCm39)	W59R	probably damaging	Het
Hdac4	A	G	1: 91,861,402 (GRCm39)	V1056A	possibly damaging	Het
Ipcef1	T	C	10: 6,850,668 (GRCm39)	T312A	probably damaging	Het
Itpr3	T	C	17: 27,331,002 (GRCm39)		probably null	Het
Kif6	A	G	17: 49,993,453 (GRCm39)	I182V	probably damaging	Het
Lcn3	A	G	2: 25,656,389 (GRCm39)	K90E	probably damaging	Het
Lig3	T	C	11: 82,681,342 (GRCm39)	S446P	probably damaging	Het
Ncln	G	A	10: 81,326,103 (GRCm39)	Q283*	probably null	Het
Nlrp4c	A	G	7: 6,069,322 (GRCm39)	T408A	probably damaging	Het
Oas1h	T	C	5: 121,009,962 (GRCm39)	F346L	probably damaging	Het
Olig2	AGCCGCCGCCGCCGCCGCAGCCGCCGCCGCCGC	AGCCGCCGCCGCCGCAGCCGCCGCCGCCGC	16: 91,023,962 (GRCm39)		probably benign	Het
Or2ad1	A	T	13: 21,326,356 (GRCm39)	Y290*	probably null	Het
Or52b4	T	C	7: 102,184,623 (GRCm39)	I223T	probably damaging	Het
Or52s1b	A	T	7: 102,822,062 (GRCm39)	F261I	probably damaging	Het
Or8b55	A	G	9: 38,727,536 (GRCm39)	I246V	probably benign	Het
Pdcd1	A	T	1: 93,969,186 (GRCm39)	V44E	probably damaging	Het
Prdm13	A	T	4: 21,679,932 (GRCm39)	I186N	unknown	Het
Prpf4b	A	G	13: 35,085,428 (GRCm39)	D958G	probably damaging	Het
Prss59	A	T	6: 40,903,022 (GRCm39)	Y117N	probably benign	Het
Prune2	A	G	19: 17,156,223 (GRCm39)	N2792S	probably damaging	Het
Robo1	T	A	16: 72,780,760 (GRCm39)	I830N	possibly damaging	Het
Scaper	A	G	9: 55,669,611 (GRCm39)	V355A	probably damaging	Het
Scn3b	C	T	9: 40,193,846 (GRCm39)	A191V	probably benign	Het
Slc13a3	G	C	2: 165,272,155 (GRCm39)	S296C	probably benign	Het
Slc6a16	T	A	7: 44,917,477 (GRCm39)	I445N	possibly damaging	Het
Snrnp35	A	G	5: 124,628,565 (GRCm39)	Y126C	possibly damaging	Het
Spock2	A	G	10: 59,957,554 (GRCm39)	H98R	possibly damaging	Het
Sptssb	A	T	3: 69,728,286 (GRCm39)	Y50*	probably null	Het
Syne1	A	G	10: 5,066,965 (GRCm39)		probably null	Het
Tcf21	T	C	10: 22,695,706 (GRCm39)	T33A	probably benign	Het
Tecta	A	G	9: 42,306,258 (GRCm39)	F57L	probably damaging	Het
Tgs1	C	A	4: 3,586,215 (GRCm39)	P364H	probably benign	Het
Tmem242	A	G	17: 5,461,711 (GRCm39)	I119T	probably benign	Het
Ttll3	CAAAGTAA	CAAAGTAAAGTAA	6: 113,376,118 (GRCm39)		probably null	Het
Vmn2r3	T	A	3: 64,186,235 (GRCm39)	N150I	probably damaging	Het
Vwf	T	A	6: 125,616,304 (GRCm39)	L1206*	probably null	Het
Zfp157	T	A	5: 138,445,833 (GRCm39)	W63R	probably benign	Het
Zfp455	T	C	13: 67,347,302 (GRCm39)	Y10H	probably benign	Het
Zfp654	T	C	16: 64,605,239 (GRCm39)	T447A	probably damaging	Het
Zhx2	T	C	15: 57,685,063 (GRCm39)	I144T	probably damaging	Het

Other mutations in Vmn2r69

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r69	APN	7	85,055,739 (GRCm39)	missense	probably benign
IGL01457:Vmn2r69	APN	7	85,055,836 (GRCm39)	missense	possibly damaging	0.87
IGL01760:Vmn2r69	APN	7	85,056,072 (GRCm39)	missense	possibly damaging	0.90
IGL01834:Vmn2r69	APN	7	85,061,576 (GRCm39)	missense	probably damaging	1.00
IGL02001:Vmn2r69	APN	7	85,056,434 (GRCm39)	missense	probably benign	0.05
IGL02057:Vmn2r69	APN	7	85,060,990 (GRCm39)	missense	possibly damaging	0.93
IGL02289:Vmn2r69	APN	7	85,056,054 (GRCm39)	missense	probably damaging	1.00
IGL02472:Vmn2r69	APN	7	85,058,960 (GRCm39)	missense	probably benign	0.01
IGL02478:Vmn2r69	APN	7	85,055,889 (GRCm39)	missense	probably damaging	1.00
IGL02554:Vmn2r69	APN	7	85,059,014 (GRCm39)	missense	probably damaging	1.00
IGL02723:Vmn2r69	APN	7	85,059,416 (GRCm39)	missense	probably damaging	1.00
R0526:Vmn2r69	UTSW	7	85,060,711 (GRCm39)	missense	probably damaging	1.00
R0560:Vmn2r69	UTSW	7	85,058,922 (GRCm39)	critical splice donor site	probably null
R0909:Vmn2r69	UTSW	7	85,055,873 (GRCm39)	missense	probably benign	0.00
R0976:Vmn2r69	UTSW	7	85,056,108 (GRCm39)	missense	probably damaging	1.00
R1158:Vmn2r69	UTSW	7	85,059,058 (GRCm39)	splice site	probably benign
R1459:Vmn2r69	UTSW	7	85,055,908 (GRCm39)	nonsense	probably null
R1482:Vmn2r69	UTSW	7	85,056,082 (GRCm39)	missense	probably damaging	1.00
R1917:Vmn2r69	UTSW	7	85,060,891 (GRCm39)	missense	probably damaging	1.00
R2016:Vmn2r69	UTSW	7	85,056,493 (GRCm39)	missense	probably damaging	0.98
R2108:Vmn2r69	UTSW	7	85,059,404 (GRCm39)	missense	probably benign
R2571:Vmn2r69	UTSW	7	85,064,764 (GRCm39)	missense	probably benign
R2910:Vmn2r69	UTSW	7	85,055,918 (GRCm39)	missense	probably damaging	1.00
R2920:Vmn2r69	UTSW	7	85,060,973 (GRCm39)	missense	probably benign	0.08
R3708:Vmn2r69	UTSW	7	85,061,029 (GRCm39)	missense	probably damaging	0.98
R3710:Vmn2r69	UTSW	7	85,055,601 (GRCm39)	missense	probably benign
R4757:Vmn2r69	UTSW	7	85,061,575 (GRCm39)	missense	probably damaging	0.99
R4823:Vmn2r69	UTSW	7	85,060,508 (GRCm39)	missense	probably benign	0.21
R4870:Vmn2r69	UTSW	7	85,060,793 (GRCm39)	missense	possibly damaging	0.93
R4918:Vmn2r69	UTSW	7	85,055,967 (GRCm39)	missense	probably benign	0.06
R5022:Vmn2r69	UTSW	7	85,060,367 (GRCm39)	missense	possibly damaging	0.72
R5174:Vmn2r69	UTSW	7	85,064,739 (GRCm39)	missense	possibly damaging	0.92
R5200:Vmn2r69	UTSW	7	85,055,717 (GRCm39)	missense	probably damaging	1.00
R5278:Vmn2r69	UTSW	7	85,060,991 (GRCm39)	missense	probably benign	0.02
R5643:Vmn2r69	UTSW	7	85,056,404 (GRCm39)	missense	probably damaging	0.98
R5996:Vmn2r69	UTSW	7	85,061,117 (GRCm39)	splice site	probably null
R6083:Vmn2r69	UTSW	7	85,055,711 (GRCm39)	missense	probably damaging	1.00
R6140:Vmn2r69	UTSW	7	85,060,657 (GRCm39)	missense	probably damaging	0.99
R6306:Vmn2r69	UTSW	7	85,064,799 (GRCm39)	missense	probably benign	0.04
R6330:Vmn2r69	UTSW	7	85,060,835 (GRCm39)	missense	probably benign
R6380:Vmn2r69	UTSW	7	85,061,067 (GRCm39)	missense	probably benign
R6466:Vmn2r69	UTSW	7	85,056,378 (GRCm39)	missense	probably benign	0.01
R6542:Vmn2r69	UTSW	7	85,060,413 (GRCm39)	nonsense	probably null
R6583:Vmn2r69	UTSW	7	85,059,017 (GRCm39)	missense	probably benign
R6623:Vmn2r69	UTSW	7	85,056,309 (GRCm39)	missense	possibly damaging	0.84
R6709:Vmn2r69	UTSW	7	85,061,069 (GRCm39)	missense	probably benign	0.03
R6732:Vmn2r69	UTSW	7	85,060,351 (GRCm39)	missense	probably benign	0.00
R6741:Vmn2r69	UTSW	7	85,061,724 (GRCm39)	missense	probably benign	0.01
R7070:Vmn2r69	UTSW	7	85,060,688 (GRCm39)	missense	probably damaging	0.98
R7234:Vmn2r69	UTSW	7	85,056,315 (GRCm39)	missense	probably benign	0.22
R7323:Vmn2r69	UTSW	7	85,060,972 (GRCm39)	missense	possibly damaging	0.95
R7427:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7428:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7453:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7532:Vmn2r69	UTSW	7	85,059,622 (GRCm39)	missense	probably benign	0.36
R7556:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7562:Vmn2r69	UTSW	7	85,056,420 (GRCm39)	missense	probably benign
R7592:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7708:Vmn2r69	UTSW	7	85,061,755 (GRCm39)	missense	possibly damaging	0.87
R7803:Vmn2r69	UTSW	7	85,056,324 (GRCm39)	missense	probably benign	0.00
R7960:Vmn2r69	UTSW	7	85,055,973 (GRCm39)	missense	probably benign
R8071:Vmn2r69	UTSW	7	85,055,713 (GRCm39)	nonsense	probably null
R8237:Vmn2r69	UTSW	7	85,060,340 (GRCm39)	missense	probably benign	0.02
R8347:Vmn2r69	UTSW	7	85,064,838 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r69	UTSW	7	85,055,783 (GRCm39)	missense	probably damaging	1.00
R8795:Vmn2r69	UTSW	7	85,064,883 (GRCm39)	start codon destroyed	probably null	0.94
R8831:Vmn2r69	UTSW	7	85,059,018 (GRCm39)	nonsense	probably null
R8856:Vmn2r69	UTSW	7	85,061,663 (GRCm39)	missense	probably benign	0.00
R8998:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R8999:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R9161:Vmn2r69	UTSW	7	85,056,177 (GRCm39)	missense	possibly damaging	0.88
R9228:Vmn2r69	UTSW	7	85,064,697 (GRCm39)	missense	probably benign	0.01
R9494:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	missense	probably damaging	1.00
R9494:Vmn2r69	UTSW	7	85,056,084 (GRCm39)	missense	probably benign	0.08
R9541:Vmn2r69	UTSW	7	85,056,209 (GRCm39)	missense	probably benign
R9620:Vmn2r69	UTSW	7	85,061,504 (GRCm39)	missense	probably benign	0.10
Z1176:Vmn2r69	UTSW	7	85,055,696 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CGATGGTGGCAATGAACATC -3'
(R):5'- GGAGTGATTGTTACAAGTGATGACC -3'

Sequencing Primer
(F):5'- TGGCAAAGGAAAATTCACTACTG -3'
(R):5'- TGTTACAAGTGATGACCAAGGAATAC -3'

Posted On

2020-09-15