Mutagenetix > Incidental Mutation

Incidental Mutation 'R9738:Clasp2'

731729

Institutional Source

Beutler Lab

Gene Symbol

Clasp2

Ensembl Gene

ENSMUSG00000033392

Gene Name

CLIP associating protein 2

Synonyms

CLASP2alpha, 1500004F14Rik, CLASP2gamma, CLASP2, CLASP2beta, 8030404L10Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9738 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

113570541-113748750 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 113590665 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 68 (L68*)

Ref Sequence

ENSEMBL: ENSMUSP00000150741 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000213663] [ENSMUST00000216817]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000213663
AA Change: L68*

Predicted Effect

probably null
Transcript: ENSMUST00000216817
AA Change: L68*

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 99.3%
20x: 98.3%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Targeted deletion of this gene leads to impaired formation of stable microtubules in a wound healing assay, and results in a 2-fold reduction of directionally persistent migration in mutant embryonic fibroblasts. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd12b	A	G	12: 70,216,039 (GRCm39)	T199A	probably benign	Het
Ank2	A	G	3: 126,737,121 (GRCm39)	F2921S	unknown	Het
Atg12	A	G	18: 46,874,505 (GRCm39)	S37P	probably benign	Het
Bnip3l	G	A	14: 67,246,214 (GRCm39)	P7L	possibly damaging	Het
Cdh5	A	G	8: 104,863,329 (GRCm39)	D413G	probably damaging	Het
Cdk8	A	G	5: 146,236,539 (GRCm39)	N318S	probably benign	Het
Cntnap2	CCACATAAACAACACACA	CCACA	6: 45,992,373 (GRCm39)		probably null	Het
Cpne9	A	C	6: 113,267,401 (GRCm39)	N194T	probably damaging	Het
Cpxm1	A	G	2: 130,235,302 (GRCm39)		probably null	Het
Crtc1	T	C	8: 70,840,205 (GRCm39)	N556S	probably damaging	Het
Gm12888	A	T	4: 121,175,520 (GRCm39)	C87*	probably null	Het
Hivep2	A	G	10: 14,019,583 (GRCm39)	E2118G	probably damaging	Het
Hrh4	A	G	18: 13,155,270 (GRCm39)	N270D	possibly damaging	Het
Iqsec1	G	A	6: 90,671,672 (GRCm39)	Q77*	probably null	Het
Lars1	A	G	18: 42,350,649 (GRCm39)	W887R	probably damaging	Het
Lgr5	A	G	10: 115,288,527 (GRCm39)	Y706H	probably damaging	Het
Ltn1	A	T	16: 87,222,524 (GRCm39)	F170I	probably damaging	Het
Msrb1	T	C	17: 24,958,535 (GRCm39)	S40P	probably damaging	Het
Myh6	T	C	14: 55,189,759 (GRCm39)	I1096V	probably benign	Het
Myh7b	A	G	2: 155,455,963 (GRCm39)	Y116C	probably damaging	Het
Ndrg2	T	C	14: 52,148,238 (GRCm39)	H41R	possibly damaging	Het
Or5an11	A	G	19: 12,245,869 (GRCm39)	T92A	probably benign	Het
Spag17	A	G	3: 99,934,526 (GRCm39)	T603A	possibly damaging	Het
Sugp1	T	A	8: 70,505,256 (GRCm39)	H74Q	probably benign	Het
Tjp1	T	C	7: 64,986,380 (GRCm39)	T243A	probably benign	Het
Tsnaxip1	A	G	8: 106,568,390 (GRCm39)	E358G	possibly damaging	Het
Unc5d	T	A	8: 29,214,332 (GRCm39)	H410L	probably benign	Het
Usp1	T	C	4: 98,819,672 (GRCm39)	V378A	probably benign	Het
Wdtc1	A	G	4: 133,022,604 (GRCm39)	S581P	probably damaging	Het
Zfp947	A	G	17: 22,365,341 (GRCm39)	V111A	probably benign	Het

Other mutations in Clasp2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00772:Clasp2	APN	9	113,735,060 (GRCm39)	splice site	probably benign
IGL00885:Clasp2	APN	9	113,740,484 (GRCm39)	missense	probably damaging	1.00
IGL01314:Clasp2	APN	9	113,735,195 (GRCm39)	missense	possibly damaging	0.89
IGL01344:Clasp2	APN	9	113,642,360 (GRCm39)	splice site	probably null
IGL01567:Clasp2	APN	9	113,709,164 (GRCm39)	missense	probably damaging	1.00
IGL02238:Clasp2	APN	9	113,709,088 (GRCm39)	missense	probably damaging	1.00
IGL02299:Clasp2	APN	9	113,709,057 (GRCm39)	missense	probably damaging	1.00
IGL02323:Clasp2	APN	9	113,697,794 (GRCm39)	splice site	probably benign
IGL02635:Clasp2	APN	9	113,737,910 (GRCm39)	missense	probably damaging	0.98
IGL02645:Clasp2	APN	9	113,719,129 (GRCm39)	missense	probably damaging	1.00
IGL02976:Clasp2	APN	9	113,735,204 (GRCm39)	missense	probably damaging	1.00
IGL03190:Clasp2	APN	9	113,673,208 (GRCm39)	nonsense	probably null
IGL03219:Clasp2	APN	9	113,677,545 (GRCm39)	splice site	probably benign
PIT4810001:Clasp2	UTSW	9	113,735,135 (GRCm39)	missense	probably damaging	1.00
R0067:Clasp2	UTSW	9	113,689,209 (GRCm39)	splice site	probably benign
R0067:Clasp2	UTSW	9	113,689,209 (GRCm39)	splice site	probably benign
R0421:Clasp2	UTSW	9	113,683,370 (GRCm39)	missense	probably benign	0.02
R0432:Clasp2	UTSW	9	113,738,487 (GRCm39)	missense	probably benign	0.00
R0458:Clasp2	UTSW	9	113,735,292 (GRCm39)	splice site	probably null
R0865:Clasp2	UTSW	9	113,740,568 (GRCm39)	missense	possibly damaging	0.57
R0972:Clasp2	UTSW	9	113,676,773 (GRCm39)	missense	possibly damaging	0.58
R1037:Clasp2	UTSW	9	113,725,702 (GRCm39)	splice site	probably benign
R1925:Clasp2	UTSW	9	113,735,265 (GRCm39)	missense	possibly damaging	0.88
R2015:Clasp2	UTSW	9	113,740,568 (GRCm39)	missense	possibly damaging	0.57
R2066:Clasp2	UTSW	9	113,735,225 (GRCm39)	missense	possibly damaging	0.86
R2330:Clasp2	UTSW	9	113,705,372 (GRCm39)	missense	probably damaging	1.00
R2568:Clasp2	UTSW	9	113,707,832 (GRCm39)	missense	probably benign
R3011:Clasp2	UTSW	9	113,730,581 (GRCm39)	missense	probably damaging	1.00
R3879:Clasp2	UTSW	9	113,719,029 (GRCm39)	missense	probably damaging	0.98
R3915:Clasp2	UTSW	9	113,737,805 (GRCm39)	missense	probably damaging	0.99
R3928:Clasp2	UTSW	9	113,735,173 (GRCm39)	missense	probably benign	0.28
R4323:Clasp2	UTSW	9	113,719,027 (GRCm39)	missense	possibly damaging	0.91
R4571:Clasp2	UTSW	9	113,676,789 (GRCm39)	missense	probably damaging	1.00
R4975:Clasp2	UTSW	9	113,732,984 (GRCm39)	missense	probably damaging	1.00
R5445:Clasp2	UTSW	9	113,733,014 (GRCm39)	missense	probably damaging	1.00
R5564:Clasp2	UTSW	9	113,641,836 (GRCm39)	critical splice donor site	probably null
R5697:Clasp2	UTSW	9	113,689,190 (GRCm39)	missense	probably benign	0.01
R5780:Clasp2	UTSW	9	113,679,220 (GRCm39)	missense	probably damaging	0.99
R5787:Clasp2	UTSW	9	113,691,310 (GRCm39)	missense	probably damaging	1.00
R6011:Clasp2	UTSW	9	113,705,315 (GRCm39)	missense	probably benign	0.07
R6026:Clasp2	UTSW	9	113,740,646 (GRCm39)	missense	probably benign	0.13
R6090:Clasp2	UTSW	9	113,681,803 (GRCm39)	missense	probably benign	0.06
R6262:Clasp2	UTSW	9	113,705,420 (GRCm39)	critical splice donor site	probably null
R6427:Clasp2	UTSW	9	113,721,512 (GRCm39)	missense	probably damaging	1.00
R6464:Clasp2	UTSW	9	113,602,785 (GRCm39)	missense	probably damaging	1.00
R6586:Clasp2	UTSW	9	113,642,332 (GRCm39)	missense	probably damaging	1.00
R6628:Clasp2	UTSW	9	113,725,788 (GRCm39)	missense	probably damaging	1.00
R6745:Clasp2	UTSW	9	113,704,338 (GRCm39)	nonsense	probably null
R7032:Clasp2	UTSW	9	113,683,391 (GRCm39)	missense	probably benign	0.04
R7165:Clasp2	UTSW	9	113,615,467 (GRCm39)	splice site	probably null
R7221:Clasp2	UTSW	9	113,681,825 (GRCm39)	missense	probably damaging	0.99
R7336:Clasp2	UTSW	9	113,705,421 (GRCm39)	splice site	probably null
R7583:Clasp2	UTSW	9	113,737,755 (GRCm39)	missense	probably benign	0.02
R7774:Clasp2	UTSW	9	113,677,804 (GRCm39)	splice site	probably null
R7895:Clasp2	UTSW	9	113,733,016 (GRCm39)	missense	probably benign	0.03
R8084:Clasp2	UTSW	9	113,676,823 (GRCm39)	missense	probably benign	0.16
R8109:Clasp2	UTSW	9	113,740,588 (GRCm39)	missense	probably damaging	1.00
R8171:Clasp2	UTSW	9	113,732,974 (GRCm39)	missense	possibly damaging	0.88
R8230:Clasp2	UTSW	9	113,721,482 (GRCm39)	missense	possibly damaging	0.73
R8810:Clasp2	UTSW	9	113,728,649 (GRCm39)	missense	probably damaging	1.00
R8879:Clasp2	UTSW	9	113,602,773 (GRCm39)	missense	probably benign	0.39
R8888:Clasp2	UTSW	9	113,732,936 (GRCm39)	missense	possibly damaging	0.54
R8889:Clasp2	UTSW	9	113,709,251 (GRCm39)	missense	probably damaging	1.00
R8892:Clasp2	UTSW	9	113,709,251 (GRCm39)	missense	probably damaging	1.00
R8922:Clasp2	UTSW	9	113,725,728 (GRCm39)	nonsense	probably null
R9042:Clasp2	UTSW	9	113,735,065 (GRCm39)	missense	probably benign
R9195:Clasp2	UTSW	9	113,671,045 (GRCm39)	missense	probably benign	0.06
R9355:Clasp2	UTSW	9	113,664,309 (GRCm39)	missense	probably damaging	1.00
R9481:Clasp2	UTSW	9	113,670,669 (GRCm39)	missense	probably damaging	1.00
R9502:Clasp2	UTSW	9	113,737,866 (GRCm39)	missense	probably benign	0.01
R9523:Clasp2	UTSW	9	113,705,372 (GRCm39)	missense	probably damaging	0.98
R9525:Clasp2	UTSW	9	113,740,677 (GRCm39)	missense	probably damaging	1.00
R9653:Clasp2	UTSW	9	113,670,993 (GRCm39)	missense	probably benign	0.01
R9699:Clasp2	UTSW	9	113,738,614 (GRCm39)	critical splice donor site	probably null
R9775:Clasp2	UTSW	9	113,725,740 (GRCm39)	missense	probably benign
X0022:Clasp2	UTSW	9	113,681,740 (GRCm39)	missense	probably damaging	1.00
Z1177:Clasp2	UTSW	9	113,599,289 (GRCm39)	missense	probably damaging	1.00
Z1177:Clasp2	UTSW	9	113,737,863 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GAAACTTGGGGTGAGGTCTAAA -3'
(R):5'- TGCCTATTTAAGAACCCTTAATGTCT -3'

Sequencing Primer
(F):5'- GGCTGACCTTGAACTCATAGAGATC -3'
(R):5'- CTGCTGAGTGCTGCGAATCTAAC -3'

Posted On

2022-11-14