Mutagenetix > Incidental Mutations

Incidental Mutation 'R0849:Tas2r109'

77063

Institutional Source

Beutler Lab

Gene Symbol

Tas2r109

Ensembl Gene

ENSMUSG00000062528

Gene Name

taste receptor, type 2, member 109

Synonyms

mt2r62, T2R09, Tas2r9, mGR09

MMRRC Submission

039028-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.048) question?

Stock #

R0849 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

132980015-132980965 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 132980893 bp

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 25 (I25V)

Ref Sequence

ENSEMBL: ENSMUSP00000069300 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000067539]

Predicted Effect

probably benign
Transcript: ENSMUST00000067539
AA Change: I25V

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000069300
Gene: ENSMUSG00000062528
AA Change: I25V

Domain	Start	End	E-Value	Type
Pfam:TAS2R	9	309	4.3e-81	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.5%
10x: 96.5%
20x: 92.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1300017J02Rik	A	C	9: 103,263,057	Y488D	possibly damaging	Het
Arhgap5	T	C	12: 52,519,623	F1126L	probably benign	Het
Arhgef11	G	T	3: 87,735,896	A1472S	probably benign	Het
Armc9	G	A	1: 86,257,270	R681K	probably benign	Het
Atxn2	T	A	5: 121,747,421		probably null	Het
B3gnt6	C	A	7: 98,194,743	L3F	probably benign	Het
Cand2	A	G	6: 115,792,391	T721A	probably damaging	Het
Cntn2	A	T	1: 132,522,386	M590K	probably benign	Het
Cntn4	A	T	6: 106,667,457	D622V	probably damaging	Het
Des	T	G	1: 75,360,628	S71A	probably benign	Het
Dnah5	C	A	15: 28,263,599	R827S	probably damaging	Het
Dstn	G	A	2: 143,938,535	G52S	probably benign	Het
Eln	T	A	5: 134,707,981	R704*	probably null	Het
Fezf2	T	A	14: 12,342,607	K419N	probably damaging	Het
Gm2381	G	A	7: 42,819,948	Q251*	probably null	Het
Gm9268	A	G	7: 43,024,718	Y400C	probably damaging	Het
Grin3a	G	A	4: 49,665,501	R1045*	probably null	Het
Herc2	T	A	7: 56,206,578	H3921Q	probably damaging	Het
Hs3st2	A	G	7: 121,501,032	E367G	possibly damaging	Het
Hydin	G	A	8: 110,598,984	R4675H	probably damaging	Het
Irf2bp1	T	C	7: 19,004,734	S100P	possibly damaging	Het
Itga10	A	G	3: 96,652,530	I500M	possibly damaging	Het
Kcnt2	T	C	1: 140,507,762	V496A	probably damaging	Het
Mansc1	T	C	6: 134,610,707	D169G	probably benign	Het
Mdn1	T	A	4: 32,741,835	S3869T	probably damaging	Het
Mrgprb4	G	A	7: 48,199,120	T20I	probably benign	Het
Nek4	A	G	14: 30,957,296	E198G	probably damaging	Het
Olfr1120	G	T	2: 87,358,265	V274L	probably benign	Het
Olfr1131	C	A	2: 87,629,282	T273K	possibly damaging	Het
Olfr1380	G	A	11: 49,564,302	C127Y	probably damaging	Het
Ppfia4	A	T	1: 134,319,372	F537I	probably benign	Het
Rarb	A	G	14: 16,434,293	V295A	probably damaging	Het
Ryr1	A	G	7: 29,040,679	S3941P	probably damaging	Het
Scnn1b	A	G	7: 121,912,475	N370S	probably damaging	Het
Stard9	C	A	2: 120,673,636	S221R	probably damaging	Het
Sv2c	C	T	13: 95,989,811	G311D	probably damaging	Het
Tbcel	A	C	9: 42,437,157	I305S	probably damaging	Het
Tmem35b	A	G	4: 127,126,197		probably null	Het
Tmem38b	T	C	4: 53,840,765	L60S	probably damaging	Het
Tmtc4	A	G	14: 122,945,554	I244T	possibly damaging	Het
Usp9y	C	A	Y: 1,394,002	C576F	probably damaging	Het
Vcan	A	T	13: 89,704,953	N629K	possibly damaging	Het
Vmn2r22	T	C	6: 123,637,404	Y409C	probably damaging	Het

Other mutations in Tas2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00467:Tas2r109	APN	6	132980023	missense	probably benign	0.04
IGL01837:Tas2r109	APN	6	132980514	missense	probably benign	0.27
IGL02094:Tas2r109	APN	6	132980239	missense	possibly damaging	0.78
R0788:Tas2r109	UTSW	6	132980301	missense	probably benign	0.01
R1542:Tas2r109	UTSW	6	132980910	missense	possibly damaging	0.93
R1583:Tas2r109	UTSW	6	132980426	missense	probably benign	0.01
R2035:Tas2r109	UTSW	6	132980460	missense	probably benign
R3845:Tas2r109	UTSW	6	132980803	missense	probably damaging	0.99
R4060:Tas2r109	UTSW	6	132980185	missense	probably damaging	1.00
R4355:Tas2r109	UTSW	6	132980181	missense	probably benign
R5353:Tas2r109	UTSW	6	132980631	missense	possibly damaging	0.61
R5860:Tas2r109	UTSW	6	132980701	missense	probably benign	0.06
R6211:Tas2r109	UTSW	6	132980624	nonsense	probably null
R6378:Tas2r109	UTSW	6	132980881	missense	probably benign	0.00
R6861:Tas2r109	UTSW	6	132980085	missense	probably benign	0.43
R7319:Tas2r109	UTSW	6	132980700	missense	probably benign	0.33
R8553:Tas2r109	UTSW	6	132980208	missense	probably benign	0.33
Z1088:Tas2r109	UTSW	6	132980301	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- AGACACTGAGGCATGTAGCAAACC -3'
(R):5'- AGCAAAGCACTTCTGGTCTGACTC -3'

Sequencing Primer
(F):5'- TGTAGCAAACCAGACACTAATATGG -3'
(R):5'- TCCTGAGCTGTGTGACAAGAC -3'

Posted On

2013-10-16