Incidental Mutation 'R0849:Tas2r109'
ID77063
Institutional Source Beutler Lab
Gene Symbol Tas2r109
Ensembl Gene ENSMUSG00000062528
Gene Nametaste receptor, type 2, member 109
Synonymsmt2r62, T2R09, Tas2r9, mGR09
MMRRC Submission 039028-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.056) question?
Stock #R0849 (G1)
Quality Score225
Status Not validated
Chromosome6
Chromosomal Location132980015-132980965 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 132980893 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Valine at position 25 (I25V)
Ref Sequence ENSEMBL: ENSMUSP00000069300 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000067539]
Predicted Effect probably benign
Transcript: ENSMUST00000067539
AA Change: I25V

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
SMART Domains Protein: ENSMUSP00000069300
Gene: ENSMUSG00000062528
AA Change: I25V

DomainStartEndE-ValueType
Pfam:TAS2R 9 309 4.3e-81 PFAM
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.5%
  • 10x: 96.5%
  • 20x: 92.5%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 43 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1300017J02Rik A C 9: 103,263,057 Y488D possibly damaging Het
Arhgap5 T C 12: 52,519,623 F1126L probably benign Het
Arhgef11 G T 3: 87,735,896 A1472S probably benign Het
Armc9 G A 1: 86,257,270 R681K probably benign Het
Atxn2 T A 5: 121,747,421 probably null Het
B3gnt6 C A 7: 98,194,743 L3F probably benign Het
Cand2 A G 6: 115,792,391 T721A probably damaging Het
Cntn2 A T 1: 132,522,386 M590K probably benign Het
Cntn4 A T 6: 106,667,457 D622V probably damaging Het
Des T G 1: 75,360,628 S71A probably benign Het
Dnah5 C A 15: 28,263,599 R827S probably damaging Het
Dstn G A 2: 143,938,535 G52S probably benign Het
Eln T A 5: 134,707,981 R704* probably null Het
Fezf2 T A 14: 12,342,607 K419N probably damaging Het
Gm2381 G A 7: 42,819,948 Q251* probably null Het
Gm9268 A G 7: 43,024,718 Y400C probably damaging Het
Grin3a G A 4: 49,665,501 R1045* probably null Het
Herc2 T A 7: 56,206,578 H3921Q probably damaging Het
Hs3st2 A G 7: 121,501,032 E367G possibly damaging Het
Hydin G A 8: 110,598,984 R4675H probably damaging Het
Irf2bp1 T C 7: 19,004,734 S100P possibly damaging Het
Itga10 A G 3: 96,652,530 I500M possibly damaging Het
Kcnt2 T C 1: 140,507,762 V496A probably damaging Het
Mansc1 T C 6: 134,610,707 D169G probably benign Het
Mdn1 T A 4: 32,741,835 S3869T probably damaging Het
Mrgprb4 G A 7: 48,199,120 T20I probably benign Het
Nek4 A G 14: 30,957,296 E198G probably damaging Het
Olfr1120 G T 2: 87,358,265 V274L probably benign Het
Olfr1131 C A 2: 87,629,282 T273K possibly damaging Het
Olfr1380 G A 11: 49,564,302 C127Y probably damaging Het
Ppfia4 A T 1: 134,319,372 F537I probably benign Het
Rarb A G 14: 16,434,293 V295A probably damaging Het
Ryr1 A G 7: 29,040,679 S3941P probably damaging Het
Scnn1b A G 7: 121,912,475 N370S probably damaging Het
Stard9 C A 2: 120,673,636 S221R probably damaging Het
Sv2c C T 13: 95,989,811 G311D probably damaging Het
Tbcel A C 9: 42,437,157 I305S probably damaging Het
Tmem35b A G 4: 127,126,197 probably null Het
Tmem38b T C 4: 53,840,765 L60S probably damaging Het
Tmtc4 A G 14: 122,945,554 I244T possibly damaging Het
Usp9y C A Y: 1,394,002 C576F probably damaging Het
Vcan A T 13: 89,704,953 N629K possibly damaging Het
Vmn2r22 T C 6: 123,637,404 Y409C probably damaging Het
Other mutations in Tas2r109
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00467:Tas2r109 APN 6 132980023 missense probably benign 0.04
IGL01837:Tas2r109 APN 6 132980514 missense probably benign 0.27
IGL02094:Tas2r109 APN 6 132980239 missense possibly damaging 0.78
R0788:Tas2r109 UTSW 6 132980301 missense probably benign 0.01
R1542:Tas2r109 UTSW 6 132980910 missense possibly damaging 0.93
R1583:Tas2r109 UTSW 6 132980426 missense probably benign 0.01
R2035:Tas2r109 UTSW 6 132980460 missense probably benign
R3845:Tas2r109 UTSW 6 132980803 missense probably damaging 0.99
R4060:Tas2r109 UTSW 6 132980185 missense probably damaging 1.00
R4355:Tas2r109 UTSW 6 132980181 missense probably benign
R5353:Tas2r109 UTSW 6 132980631 missense possibly damaging 0.61
R5860:Tas2r109 UTSW 6 132980701 missense probably benign 0.06
R6211:Tas2r109 UTSW 6 132980624 nonsense probably null
R6378:Tas2r109 UTSW 6 132980881 missense probably benign 0.00
R6861:Tas2r109 UTSW 6 132980085 missense probably benign 0.43
R7319:Tas2r109 UTSW 6 132980700 missense probably benign 0.33
Z1088:Tas2r109 UTSW 6 132980301 missense probably benign 0.07
Predicted Primers PCR Primer
(F):5'- AGACACTGAGGCATGTAGCAAACC -3'
(R):5'- AGCAAAGCACTTCTGGTCTGACTC -3'

Sequencing Primer
(F):5'- TGTAGCAAACCAGACACTAATATGG -3'
(R):5'- TCCTGAGCTGTGTGACAAGAC -3'
Posted On2013-10-16