Incidental Mutation 'R2877:Gpr25'
ID 260581
Institutional Source Beutler Lab
Gene Symbol Gpr25
Ensembl Gene ENSMUSG00000052759
Gene Name G protein-coupled receptor 25
Synonyms LOC383563
MMRRC Submission 040465-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R2877 (G1)
Quality Score 225
Status Validated
Chromosome 1
Chromosomal Location 136187535-136188611 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to T at 136188553 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glycine to Aspartic acid at position 20 (G20D)
Ref Sequence ENSEMBL: ENSMUSP00000083583 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000086395]
AlphaFold P0C5I1
Predicted Effect possibly damaging
Transcript: ENSMUST00000086395
AA Change: G20D

PolyPhen 2 Score 0.864 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000083583
Gene: ENSMUSG00000052759
AA Change: G20D

Pfam:7tm_1 56 304 1.1e-40 PFAM
low complexity region 328 342 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000181385
Predicted Effect noncoding transcript
Transcript: ENSMUST00000193939
Meta Mutation Damage Score 0.1795 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.4%
  • 20x: 95.4%
Validation Efficiency 96% (66/69)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. This gene has been linked to arterial stiffness. [provided by RefSeq, Nov 2012]
Allele List at MGI
Other mutations in this stock
Total: 58 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930522L14Rik A C 5: 109,886,811 (GRCm39) probably benign Het
Abca13 C T 11: 9,241,889 (GRCm39) L1251F possibly damaging Het
Accsl A T 2: 93,689,755 (GRCm39) M384K probably damaging Het
Adss1 A C 12: 112,600,623 (GRCm39) K197N probably damaging Het
Alg11 A G 8: 22,555,374 (GRCm39) N170D possibly damaging Het
Ambn T C 5: 88,608,559 (GRCm39) probably benign Het
Anapc7 T C 5: 122,566,219 (GRCm39) Y43H probably benign Het
Anxa10 T A 8: 62,513,373 (GRCm39) I255F probably damaging Het
Atg2b A T 12: 105,630,268 (GRCm39) Y374* probably null Het
Axl T A 7: 25,465,949 (GRCm39) M563L probably damaging Het
Carmil2 A G 8: 106,422,055 (GRCm39) E1108G probably damaging Het
Casp1 T C 9: 5,303,110 (GRCm39) M188T probably damaging Het
Chd3 A T 11: 69,251,998 (GRCm39) C87* probably null Het
Col6a3 G A 1: 90,703,321 (GRCm39) T2475I unknown Het
Creb3l4 A T 3: 90,149,615 (GRCm39) S83R probably damaging Het
Cyp2a4 G A 7: 26,011,612 (GRCm39) E278K possibly damaging Het
Dync1li2 A C 8: 105,156,047 (GRCm39) Y265D probably damaging Het
Eif3f G A 7: 108,534,019 (GRCm39) probably null Het
Eipr1 C T 12: 28,810,091 (GRCm39) T22I possibly damaging Het
Fbxo48 A G 11: 16,903,382 (GRCm39) K3E possibly damaging Het
Fbxw13 A G 9: 109,010,534 (GRCm39) F368S probably damaging Het
Fbxw19 A T 9: 109,315,038 (GRCm39) W175R probably damaging Het
Fibcd1 G A 2: 31,728,678 (GRCm39) P60S probably benign Het
Foxa3 A T 7: 18,748,805 (GRCm39) M107K probably benign Het
Foxj2 G A 6: 122,819,791 (GRCm39) D560N probably damaging Het
Gfm2 A G 13: 97,289,757 (GRCm39) R181G possibly damaging Het
Grin1 A G 2: 25,187,641 (GRCm39) V594A probably damaging Het
Itpripl1 A G 2: 126,983,534 (GRCm39) V196A probably benign Het
Kcns1 A G 2: 164,006,682 (GRCm39) I427T probably damaging Het
Kiz T C 2: 146,731,476 (GRCm39) V322A possibly damaging Het
Mslnl G A 17: 25,961,908 (GRCm39) V128M probably damaging Het
Muc19 T A 15: 91,777,200 (GRCm39) noncoding transcript Het
Naa16 A G 14: 79,580,738 (GRCm39) M592T probably benign Het
Naa80 A T 9: 107,460,367 (GRCm39) E87D possibly damaging Het
Ncapd3 T A 9: 26,955,783 (GRCm39) probably null Het
Nebl C A 2: 17,439,740 (GRCm39) D178Y probably damaging Het
Nedd1 T A 10: 92,549,988 (GRCm39) N99I possibly damaging Het
Nuak1 T C 10: 84,211,209 (GRCm39) D293G possibly damaging Het
Or5b123 A T 19: 13,596,996 (GRCm39) I157F probably damaging Het
Or5m9b T C 2: 85,905,675 (GRCm39) M197T possibly damaging Het
Palb2 A G 7: 121,713,652 (GRCm39) V877A probably damaging Het
Rassf6 C T 5: 90,754,664 (GRCm39) V205I probably damaging Het
Rbak A G 5: 143,159,860 (GRCm39) Y398H probably damaging Het
Rcbtb1 A G 14: 59,448,041 (GRCm39) probably benign Het
Robo4 CGG CG 9: 37,322,786 (GRCm39) probably null Het
Smpdl3a C A 10: 57,685,181 (GRCm39) T317K probably damaging Het
Speer4e1 C T 5: 14,987,130 (GRCm39) V92M probably damaging Het
Syne2 A G 12: 76,047,605 (GRCm39) I4009V probably benign Het
Tarbp1 G A 8: 127,154,571 (GRCm39) L1474F probably damaging Het
Tchh A G 3: 93,351,535 (GRCm39) E325G unknown Het
Trpc4 T C 3: 54,198,761 (GRCm39) S562P probably damaging Het
Ttn T C 2: 76,567,409 (GRCm39) D27828G probably damaging Het
Ulk4 T C 9: 121,089,105 (GRCm39) D258G probably benign Het
Vmn1r215 A G 13: 23,260,731 (GRCm39) H257R probably benign Het
Vmn2r56 A C 7: 12,444,954 (GRCm39) M433R probably benign Het
Vmn2r76 C A 7: 85,875,201 (GRCm39) C592F probably benign Het
Zcchc8 C T 5: 123,838,766 (GRCm39) V591I probably benign Het
Znhit6 G C 3: 145,282,409 (GRCm39) G96A probably benign Het
Other mutations in Gpr25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03028:Gpr25 APN 1 136,188,550 (GRCm39) missense probably benign 0.00
R1728:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1729:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1732:Gpr25 UTSW 1 136,187,866 (GRCm39) missense probably benign 0.01
R1739:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1762:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1783:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1785:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1907:Gpr25 UTSW 1 136,188,538 (GRCm39) missense probably benign 0.00
R3081:Gpr25 UTSW 1 136,187,623 (GRCm39) missense possibly damaging 0.68
R5132:Gpr25 UTSW 1 136,188,103 (GRCm39) missense probably damaging 1.00
R7133:Gpr25 UTSW 1 136,188,559 (GRCm39) missense probably damaging 1.00
R8163:Gpr25 UTSW 1 136,187,596 (GRCm39) missense probably damaging 1.00
R8248:Gpr25 UTSW 1 136,188,415 (GRCm39) missense probably benign 0.03
R9051:Gpr25 UTSW 1 136,188,026 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2015-01-23