Mutagenetix > Incidental Mutation

Incidental Mutation 'R6682:Zfp9'

527540

Institutional Source

Beutler Lab

Gene Symbol

Zfp9

Ensembl Gene

ENSMUSG00000072623

Gene Name

zinc finger protein 9

Synonyms

Zfp-9, Krox-4, 1810048F22Rik

MMRRC Submission

044801-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.428) question?

Stock #

R6682 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

118438911-118456281 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 118444202 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 47 (V47A)

Ref Sequence

ENSEMBL: ENSMUSP00000123810 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000161170]

AlphaFold

Q8BIS1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000161170
AA Change: V47A

PolyPhen 2 Score 0.825 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000123810
Gene: ENSMUSG00000072623
AA Change: V47A

Domain	Start	End	E-Value	Type
KRAB	8	68	2.26e-31	SMART
ZnF_C2H2	117	139	1.82e-3	SMART
ZnF_C2H2	144	166	4.17e-3	SMART
ZnF_C2H2	172	194	5.99e-4	SMART
ZnF_C2H2	200	222	2.79e-4	SMART
ZnF_C2H2	228	250	1.56e-2	SMART
ZnF_C2H2	256	278	6.88e-4	SMART
ZnF_C2H2	284	306	7.9e-4	SMART
ZnF_C2H2	312	334	2.75e-3	SMART
ZnF_C2H2	340	362	5.14e-3	SMART
ZnF_C2H2	368	390	1.26e-2	SMART
ZnF_C2H2	396	418	1.6e-4	SMART
ZnF_C2H2	424	446	9.08e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204066

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aspm	T	C	1: 139,385,460 (GRCm39)	V368A	possibly damaging	Het
Bcl9l	A	G	9: 44,412,400 (GRCm39)	T92A	possibly damaging	Het
Celsr2	A	T	3: 108,307,817 (GRCm39)		probably null	Het
Cndp2	T	C	18: 84,695,455 (GRCm39)	K149E	probably benign	Het
Cnpy4	T	C	5: 138,185,984 (GRCm39)		probably null	Het
Cox6c	A	T	15: 35,938,319 (GRCm39)		probably null	Het
Cpt2	G	T	4: 107,761,627 (GRCm39)	S158R	probably damaging	Het
Dlgap1	A	G	17: 71,094,118 (GRCm39)	K813R	probably damaging	Het
Dock10	A	C	1: 80,490,338 (GRCm39)	L1927R	probably damaging	Het
Gak	T	A	5: 108,746,742 (GRCm39)	K430I	probably damaging	Het
Grik3	T	A	4: 125,544,259 (GRCm39)	Y327N	probably damaging	Het
Ldb3	T	A	14: 34,274,221 (GRCm39)	T334S	possibly damaging	Het
Ldlr	A	G	9: 21,643,671 (GRCm39)	D85G	probably benign	Het
Med26	T	A	8: 73,249,927 (GRCm39)	T391S	probably benign	Het
Mmp27	A	G	9: 7,573,606 (GRCm39)	T233A	probably benign	Het
Mob1a	A	G	6: 83,311,132 (GRCm39)	Y117C	possibly damaging	Het
Mrps35	A	T	6: 146,949,777 (GRCm39)	E97V	possibly damaging	Het
Msln	A	T	17: 25,971,993 (GRCm39)	S75T	probably damaging	Het
Myoz1	C	T	14: 20,703,687 (GRCm39)		probably null	Het
Nim1k	A	G	13: 120,173,724 (GRCm39)	I390T	probably benign	Het
Or10k2	A	G	8: 84,268,187 (GRCm39)	H138R	probably benign	Het
Or4k51	C	T	2: 111,584,980 (GRCm39)	P129S	probably damaging	Het
Pclo	A	C	5: 14,589,893 (GRCm39)	Q731P	unknown	Het
Prl3d3	G	A	13: 27,345,023 (GRCm39)	E132K	probably benign	Het
Pth1r	C	T	9: 110,556,319 (GRCm39)		probably null	Het
Ptpru	T	C	4: 131,548,093 (GRCm39)	M135V	probably benign	Het
Slc12a9	A	T	5: 137,325,663 (GRCm39)	L316Q	probably damaging	Het
Slc35f6	G	A	5: 30,814,764 (GRCm39)	M177I	possibly damaging	Het
Smc4	T	A	3: 68,914,574 (GRCm39)	S62R	probably damaging	Het
Tmem179	C	T	12: 112,469,714 (GRCm39)	D29N	probably benign	Het
Togaram2	A	T	17: 72,011,749 (GRCm39)	D476V	probably benign	Het
Trpc4ap	C	T	2: 155,479,687 (GRCm39)		probably null	Het
Trpm8	C	A	1: 88,254,224 (GRCm39)	T149K	probably damaging	Het
Uhmk1	C	T	1: 170,039,804 (GRCm39)		probably null	Het
Vmn2r79	C	A	7: 86,653,370 (GRCm39)	T545K	possibly damaging	Het
Vmn2r95	C	A	17: 18,660,489 (GRCm39)	N300K	probably damaging	Het
Wdr41	G	A	13: 95,149,639 (GRCm39)	G419D	probably damaging	Het
Zc3hav1	A	T	6: 38,302,130 (GRCm39)	H597Q	probably benign	Het
Zfp704	T	C	3: 9,630,253 (GRCm39)	E36G	probably benign	Het

Other mutations in Zfp9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00336:Zfp9	APN	6	118,441,436 (GRCm39)	missense	probably damaging	1.00
IGL01093:Zfp9	APN	6	118,442,800 (GRCm39)	missense	probably benign	0.14
IGL01512:Zfp9	APN	6	118,444,292 (GRCm39)	missense	probably damaging	1.00
R0491:Zfp9	UTSW	6	118,442,163 (GRCm39)	missense	probably damaging	0.96
R1716:Zfp9	UTSW	6	118,441,712 (GRCm39)	missense	probably damaging	1.00
R1858:Zfp9	UTSW	6	118,442,021 (GRCm39)	missense	probably benign	0.15
R4088:Zfp9	UTSW	6	118,441,730 (GRCm39)	missense	probably damaging	1.00
R4490:Zfp9	UTSW	6	118,442,273 (GRCm39)	missense	probably benign	0.06
R4627:Zfp9	UTSW	6	118,441,937 (GRCm39)	missense	probably damaging	1.00
R4951:Zfp9	UTSW	6	118,441,408 (GRCm39)	missense	probably damaging	1.00
R5344:Zfp9	UTSW	6	118,442,140 (GRCm39)	missense	probably damaging	1.00
R5594:Zfp9	UTSW	6	118,442,000 (GRCm39)	missense	probably damaging	0.98
R6198:Zfp9	UTSW	6	118,454,282 (GRCm39)	start codon destroyed	probably null
R6986:Zfp9	UTSW	6	118,441,426 (GRCm39)	missense	possibly damaging	0.77
R7147:Zfp9	UTSW	6	118,441,963 (GRCm39)	missense	probably damaging	1.00
R7799:Zfp9	UTSW	6	118,441,843 (GRCm39)	missense	probably damaging	1.00
R7921:Zfp9	UTSW	6	118,442,032 (GRCm39)	missense	possibly damaging	0.70
R7934:Zfp9	UTSW	6	118,441,847 (GRCm39)	missense	probably damaging	0.99
R8111:Zfp9	UTSW	6	118,441,561 (GRCm39)	missense	probably damaging	1.00
R8369:Zfp9	UTSW	6	118,441,353 (GRCm39)	missense	probably damaging	0.99
R8916:Zfp9	UTSW	6	118,442,223 (GRCm39)	nonsense	probably null
R9660:Zfp9	UTSW	6	118,443,851 (GRCm39)	missense	probably benign
R9728:Zfp9	UTSW	6	118,443,851 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACACATTCCTTGGGAGAGAGG -3'
(R):5'- ACCTTTGTGCCTTCCTGAAG -3'

Sequencing Primer
(F):5'- CTGGTATCCCCAAACAGATG -3'
(R):5'- TCCTGAAGTCACTTCACACTGAG -3'

Posted On

2018-07-23