Mutagenetix > Incidental Mutation

Incidental Mutation 'R8551:Zfp735'

659872

Institutional Source

Beutler Lab

Gene Symbol

Zfp735

Ensembl Gene

ENSMUSG00000060630

Gene Name

zinc finger protein 735

Synonyms

1700012C15Rik

MMRRC Submission

068516-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R8551 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

73579604-73604624 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 73603122 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 689 (I689V)

Ref Sequence

ENSEMBL: ENSMUSP00000079269 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080407]

AlphaFold

B1ARH2

Predicted Effect

probably benign
Transcript: ENSMUST00000080407
AA Change: I689V

PolyPhen 2 Score 0.061 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000079269
Gene: ENSMUSG00000060630
AA Change: I689V

Domain	Start	End	E-Value	Type
KRAB	8	68	2.2e-34	SMART
ZnF_C2H2	483	505	4.38e1	SMART
ZnF_C2H2	511	533	2.67e-1	SMART
ZnF_C2H2	539	561	1.81e1	SMART
ZnF_C2H2	567	589	1.5e-4	SMART
ZnF_C2H2	595	617	4.87e-4	SMART
ZnF_C2H2	623	645	4.24e-4	SMART
ZnF_C2H2	651	673	2.27e-4	SMART
ZnF_C2H2	679	701	7.49e-5	SMART
ZnF_C2H2	707	729	4.87e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

98% (39/40)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamtsl3	G	A	7: 82,189,678 (GRCm39)	R634H	probably benign	Het
Birc6	T	G	17: 74,864,949 (GRCm39)	S236R	probably benign	Het
Dnah12	T	A	14: 26,496,227 (GRCm39)	I1493K	probably damaging	Het
Dnah7b	T	C	1: 46,155,360 (GRCm39)	S303P	possibly damaging	Het
Eri2	A	T	7: 119,387,062 (GRCm39)		probably null	Het
Erlec1	C	A	11: 30,881,829 (GRCm39)	C467F	probably damaging	Het
Exph5	A	G	9: 53,285,351 (GRCm39)	T811A	possibly damaging	Het
Galr1	T	A	18: 82,424,191 (GRCm39)	I29F	probably benign	Het
Gata3	C	A	2: 9,867,994 (GRCm39)	C320F	probably damaging	Het
Ggnbp2	A	T	11: 84,732,351 (GRCm39)	Y247*	probably null	Het
Gnao1	A	G	8: 94,682,735 (GRCm39)	Q307R	probably damaging	Het
Hbp1	T	C	12: 31,980,709 (GRCm39)	T465A	probably damaging	Het
Liph	C	G	16: 21,800,158 (GRCm39)	G152R	probably damaging	Het
Lrguk	T	C	6: 34,093,446 (GRCm39)	S656P	probably damaging	Het
Lyrm4	T	A	13: 36,163,844 (GRCm39)	N85I	probably benign	Het
Lyst	T	A	13: 13,808,645 (GRCm39)	I105N	possibly damaging	Het
Maml3	T	C	3: 51,764,488 (GRCm39)	T159A	probably benign	Het
Man1c1	A	T	4: 134,430,326 (GRCm39)	L152*	probably null	Het
Map3k20	T	G	2: 72,232,704 (GRCm39)		probably benign	Het
Mthfsl	G	T	9: 88,570,943 (GRCm39)	R102S	possibly damaging	Het
Myo3a	T	A	2: 22,337,277 (GRCm39)	S391R	probably benign	Het
Naa50	A	G	16: 43,979,996 (GRCm39)	D128G	probably benign	Het
Nlrp14	C	T	7: 106,782,359 (GRCm39)	R519C	possibly damaging	Het
Or5b119	A	G	19: 13,457,109 (GRCm39)	I151T	possibly damaging	Het
Or6d14	G	T	6: 116,534,289 (GRCm39)	R301L	probably damaging	Het
Oxtr	T	C	6: 112,465,939 (GRCm39)	K274E	probably damaging	Het
Prkn	A	G	17: 11,286,103 (GRCm39)	K32R	probably damaging	Het
Relt	T	C	7: 100,512,409 (GRCm39)		probably benign	Het
Rftn1	G	T	17: 50,354,408 (GRCm39)	A318D	probably damaging	Het
Ryr2	T	A	13: 11,575,479 (GRCm39)	I4840F	possibly damaging	Het
Sccpdh	T	A	1: 179,509,013 (GRCm39)	Y27N	probably damaging	Het
Slc7a5	A	G	8: 122,613,050 (GRCm39)	S343P	probably damaging	Het
Snrnp200	A	G	2: 127,068,971 (GRCm39)	D950G	probably benign	Het
Stk38	A	G	17: 29,207,199 (GRCm39)	Y115H	probably damaging	Het
Tas2r116	T	C	6: 132,832,993 (GRCm39)	V198A	probably benign	Het
Tfap2d	A	G	1: 19,175,024 (GRCm39)	D159G	probably benign	Het
Trim69	A	G	2: 122,003,810 (GRCm39)	D253G	probably benign	Het
Trpv4	A	T	5: 114,768,900 (GRCm39)	F359I	possibly damaging	Het
Twist1	C	T	12: 34,008,103 (GRCm39)	R43W	unknown	Het
Usp19	A	T	9: 108,376,496 (GRCm39)	E1026V	possibly damaging	Het
Virma	A	G	4: 11,513,397 (GRCm39)	Y417C	probably damaging	Het

Other mutations in Zfp735

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00492:Zfp735	APN	11	73,602,192 (GRCm39)	missense	possibly damaging	0.86
IGL00798:Zfp735	APN	11	73,602,386 (GRCm39)	missense	possibly damaging	0.72
IGL01642:Zfp735	APN	11	73,601,305 (GRCm39)	missense	possibly damaging	0.73
IGL01684:Zfp735	APN	11	73,581,191 (GRCm39)	missense	possibly damaging	0.86
IGL02096:Zfp735	APN	11	73,602,254 (GRCm39)	missense	probably benign	0.01
IGL02238:Zfp735	APN	11	73,601,319 (GRCm39)	missense	probably benign	0.00
IGL02505:Zfp735	APN	11	73,580,626 (GRCm39)	missense	probably benign	0.03
IGL02740:Zfp735	APN	11	73,601,412 (GRCm39)	missense	possibly damaging	0.53
IGL02957:Zfp735	APN	11	73,601,755 (GRCm39)	missense	probably benign	0.00
bananaquit	UTSW	11	73,601,412 (GRCm39)	nonsense	probably null
bescher	UTSW	11	73,602,979 (GRCm39)	missense	possibly damaging	0.93
Galvanic	UTSW	11	73,602,504 (GRCm39)	nonsense	probably null
grassquit	UTSW	11	73,603,029 (GRCm39)	missense	possibly damaging	0.66
R0114:Zfp735	UTSW	11	73,601,488 (GRCm39)	missense	probably benign	0.33
R0217:Zfp735	UTSW	11	73,602,112 (GRCm39)	missense	possibly damaging	0.73
R0943:Zfp735	UTSW	11	73,602,909 (GRCm39)	missense	probably benign	0.04
R1421:Zfp735	UTSW	11	73,601,523 (GRCm39)	missense	probably benign
R1460:Zfp735	UTSW	11	73,603,159 (GRCm39)	missense	possibly damaging	0.73
R1493:Zfp735	UTSW	11	73,601,305 (GRCm39)	missense	possibly damaging	0.73
R1517:Zfp735	UTSW	11	73,601,470 (GRCm39)	missense	probably benign
R1676:Zfp735	UTSW	11	73,602,301 (GRCm39)	missense	possibly damaging	0.53
R1709:Zfp735	UTSW	11	73,602,589 (GRCm39)	missense	probably benign	0.01
R1871:Zfp735	UTSW	11	73,601,412 (GRCm39)	nonsense	probably null
R1931:Zfp735	UTSW	11	73,602,677 (GRCm39)	missense	possibly damaging	0.69
R2219:Zfp735	UTSW	11	73,601,851 (GRCm39)	missense	possibly damaging	0.53
R2227:Zfp735	UTSW	11	73,602,223 (GRCm39)	nonsense	probably null
R2227:Zfp735	UTSW	11	73,602,222 (GRCm39)	missense	possibly damaging	0.53
R3552:Zfp735	UTSW	11	73,602,067 (GRCm39)	nonsense	probably null
R3856:Zfp735	UTSW	11	73,602,282 (GRCm39)	missense	probably benign	0.01
R3925:Zfp735	UTSW	11	73,601,950 (GRCm39)	missense	probably benign	0.33
R4572:Zfp735	UTSW	11	73,580,611 (GRCm39)	missense	probably benign	0.02
R4585:Zfp735	UTSW	11	73,580,550 (GRCm39)	missense	possibly damaging	0.51
R4586:Zfp735	UTSW	11	73,580,550 (GRCm39)	missense	possibly damaging	0.51
R4619:Zfp735	UTSW	11	73,602,031 (GRCm39)	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73,602,682 (GRCm39)	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73,602,681 (GRCm39)	missense	probably damaging	0.98
R5435:Zfp735	UTSW	11	73,602,939 (GRCm39)	missense	possibly damaging	0.72
R5489:Zfp735	UTSW	11	73,601,419 (GRCm39)	nonsense	probably null
R5516:Zfp735	UTSW	11	73,601,640 (GRCm39)	missense	probably benign
R5654:Zfp735	UTSW	11	73,602,964 (GRCm39)	missense	possibly damaging	0.71
R5990:Zfp735	UTSW	11	73,581,174 (GRCm39)	missense	possibly damaging	0.70
R6332:Zfp735	UTSW	11	73,602,504 (GRCm39)	nonsense	probably null
R6427:Zfp735	UTSW	11	73,581,140 (GRCm39)	missense	possibly damaging	0.73
R6460:Zfp735	UTSW	11	73,602,478 (GRCm39)	missense	probably benign	0.33
R6820:Zfp735	UTSW	11	73,579,783 (GRCm39)	start codon destroyed	probably null	0.01
R6831:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6833:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6834:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6897:Zfp735	UTSW	11	73,601,880 (GRCm39)	missense	probably benign	0.08
R6941:Zfp735	UTSW	11	73,581,159 (GRCm39)	missense	probably benign	0.33
R7335:Zfp735	UTSW	11	73,602,379 (GRCm39)	missense	possibly damaging	0.47
R7366:Zfp735	UTSW	11	73,602,979 (GRCm39)	missense	possibly damaging	0.93
R7474:Zfp735	UTSW	11	73,602,002 (GRCm39)	missense	possibly damaging	0.72
R7487:Zfp735	UTSW	11	73,581,154 (GRCm39)	missense	possibly damaging	0.53
R7583:Zfp735	UTSW	11	73,601,933 (GRCm39)	missense	possibly damaging	0.86
R7866:Zfp735	UTSW	11	73,601,629 (GRCm39)	missense	probably benign	0.00
R8005:Zfp735	UTSW	11	73,603,140 (GRCm39)	nonsense	probably null
R8500:Zfp735	UTSW	11	73,601,811 (GRCm39)	missense	possibly damaging	0.53
R8754:Zfp735	UTSW	11	73,603,000 (GRCm39)	missense	possibly damaging	0.85
R8769:Zfp735	UTSW	11	73,581,127 (GRCm39)	missense	possibly damaging	0.53
R8794:Zfp735	UTSW	11	73,603,029 (GRCm39)	missense	possibly damaging	0.66
R8835:Zfp735	UTSW	11	73,601,692 (GRCm39)	missense	possibly damaging	0.53
R8869:Zfp735	UTSW	11	73,602,510 (GRCm39)	missense	possibly damaging	0.53
R8969:Zfp735	UTSW	11	73,602,699 (GRCm39)	missense	possibly damaging	0.83
R9072:Zfp735	UTSW	11	73,603,060 (GRCm39)	missense	probably benign	0.21
R9073:Zfp735	UTSW	11	73,603,060 (GRCm39)	missense	probably benign	0.21
R9193:Zfp735	UTSW	11	73,580,600 (GRCm39)	missense	possibly damaging	0.71
R9355:Zfp735	UTSW	11	73,602,362 (GRCm39)	missense	probably benign	0.01
R9414:Zfp735	UTSW	11	73,602,023 (GRCm39)	nonsense	probably null
R9456:Zfp735	UTSW	11	73,602,403 (GRCm39)	missense	possibly damaging	0.53
R9573:Zfp735	UTSW	11	73,602,936 (GRCm39)	missense	possibly damaging	0.67
R9647:Zfp735	UTSW	11	73,580,600 (GRCm39)	missense	probably damaging	0.98
R9710:Zfp735	UTSW	11	73,601,806 (GRCm39)	missense	possibly damaging	0.86
Z1176:Zfp735	UTSW	11	73,601,641 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GCTCAGTACTCAGCTCTTCAAAA -3'
(R):5'- AATCATGTAAATGATTCCCCATGC -3'

Sequencing Primer
(F):5'- TCTTCAAGTGCATCAGAGAGTCC -3'
(R):5'- GAAGTCTGTGGTGAACTTTAAGAC -3'

Posted On

2021-01-18