Mutagenetix > Incidental Mutation

Incidental Mutation 'R8676:Tmem89'

661394

Institutional Source

Beutler Lab

Gene Symbol

Tmem89

Ensembl Gene

ENSMUSG00000025652

Gene Name

transmembrane protein 89

Synonyms

1700024B07Rik

MMRRC Submission

068531-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R8676 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

108914619-108915563 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 108915027 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 132 (L132F)

Ref Sequence

ENSEMBL: ENSMUSP00000142016 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026744] [ENSMUST00000098376] [ENSMUST00000192852]

AlphaFold

Q9DA04

Predicted Effect

probably benign
Transcript: ENSMUST00000026744

SMART Domains

Protein: ENSMUSP00000026744
Gene: ENSMUSG00000025652

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:TMEM89	23	47	6.3e-16	PFAM
Pfam:TMEM89	46	166	1.1e-62	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000098376

SMART Domains

Protein: ENSMUSP00000095979
Gene: ENSMUSG00000023259

Domain	Start	End	E-Value	Type
Pfam:Sulfate_transp	69	458	4.4e-113	PFAM
low complexity region	471	484	N/A	INTRINSIC
PDB:3LLO\|A	489	546	2e-16	PDB
low complexity region	554	593	N/A	INTRINSIC
low complexity region	638	650	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000191404

Predicted Effect

unknown
Transcript: ENSMUST00000192852
AA Change: L132F

SMART Domains

Protein: ENSMUSP00000142016
Gene: ENSMUSG00000025652
AA Change: L132F

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:TMEM89	23	47	1.8e-15	PFAM
Pfam:TMEM89	45	118	1.3e-37	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

93% (54/58)

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610037L13Rik	A	G	4: 107,895,599 (GRCm38)	N152D	unknown	Het
1700021F05Rik	A	G	10: 43,532,937 (GRCm38)	L70S	probably benign	Het
Acsm3	T	A	7: 119,775,169 (GRCm38)	S281R	probably damaging	Het
Adipor2	G	T	6: 119,363,486 (GRCm38)		probably benign	Het
Alk	T	C	17: 71,897,941 (GRCm38)	S1079G	probably damaging	Het
Ankrd27	T	C	7: 35,602,584 (GRCm38)		probably null	Het
Anxa6	C	A	11: 55,001,282 (GRCm38)	E283*	probably null	Het
Bnc2	T	C	4: 84,276,313 (GRCm38)	H858R	possibly damaging	Het
Btnl6	T	C	17: 34,508,069 (GRCm38)	S496G	probably benign	Het
Ccdc88a	T	C	11: 29,460,860 (GRCm38)	S449P	probably benign	Het
Cdh23	A	T	10: 60,410,910 (GRCm38)	D916E	probably damaging	Het
Cfap43	A	T	19: 47,748,017 (GRCm38)	L1345H	possibly damaging	Het
Cyld	A	T	8: 88,729,510 (GRCm38)	H396L	probably benign	Het
Cyp20a1	A	G	1: 60,379,420 (GRCm38)	T340A	possibly damaging	Het
Dera	A	T	6: 137,830,204 (GRCm38)	I217F	probably damaging	Het
Dnah11	A	G	12: 118,190,804 (GRCm38)	L247P	probably damaging	Het
Eftud2	G	A	11: 102,868,621 (GRCm38)	T152M	probably damaging	Het
Epb41l2	C	T	10: 25,443,776 (GRCm38)	T169M	probably benign	Het
Fam186a	T	C	15: 99,947,142 (GRCm38)	D407G	unknown	Het
Fam189a2	T	C	19: 23,988,494 (GRCm38)	K214E	probably damaging	Het
Gli3	T	A	13: 15,715,034 (GRCm38)	C578S	probably damaging	Het
Gm436	G	A	4: 144,670,113 (GRCm38)	R350C	possibly damaging	Het
Gm6408	A	G	5: 146,482,427 (GRCm38)	N84S	probably benign	Het
Heatr5a	AGCACACTGCAGGAAGCTCACACAGCACAGCATACCTTCAGGAGTGCACACTGCAGGAAGCTCACACAGCACAGCATACCTTCAGGAGAGCACACTGCAGGAAGCTCA	AGCACACTGCAGGAAGCTCACACAGCACAGCATACCTTCAGGAGAGCACACTGCAGGAAGCTCA	12: 51,887,919 (GRCm38)		probably benign	Het
Herc2	C	A	7: 56,188,613 (GRCm38)	T3296K	probably damaging	Het
Hnf4g	A	T	3: 3,643,073 (GRCm38)		probably benign	Het
Hyal4	C	A	6: 24,755,827 (GRCm38)	Q15K	probably damaging	Het
Itpr3	G	A	17: 27,118,677 (GRCm38)		probably benign	Het
Kcna3	A	G	3: 107,036,592 (GRCm38)	E57G	probably damaging	Het
Kcnc3	C	A	7: 44,591,596 (GRCm38)	D237E	probably benign	Het
Map3k8	A	G	18: 4,343,137 (GRCm38)	V130A	probably benign	Het
Mpp7	A	G	18: 7,440,430 (GRCm38)		probably null	Het
Myh13	T	A	11: 67,342,485 (GRCm38)	L610Q	probably damaging	Het
Olfr1020	T	A	2: 85,849,902 (GRCm38)	M150K	probably benign	Het
Olfr1339	C	A	4: 118,735,038 (GRCm38)	P170T	probably damaging	Het
Olfr917	T	C	9: 38,665,768 (GRCm38)	I25M	probably benign	Het
Pcdhb5	A	T	18: 37,321,076 (GRCm38)	T170S	probably benign	Het
Polr3b	T	A	10: 84,680,387 (GRCm38)	H626Q	probably benign	Het
Prkg1	A	T	19: 31,764,746 (GRCm38)	L26Q	probably damaging	Het
Prob1	T	C	18: 35,653,986 (GRCm38)	N405S	possibly damaging	Het
Proz	T	G	8: 13,073,630 (GRCm38)	S300R	probably damaging	Het
Psg19	A	G	7: 18,794,065 (GRCm38)	I251T	probably benign	Het
Rcbtb1	T	C	14: 59,229,952 (GRCm38)	I413T	possibly damaging	Het
Rnf144b	T	A	13: 47,228,976 (GRCm38)	Y103N	probably damaging	Het
Rspry1	G	C	8: 94,632,119 (GRCm38)	G194R	probably benign	Het
Scn2b	A	G	9: 45,125,619 (GRCm38)	I142V	probably damaging	Het
Spata20	A	T	11: 94,481,781 (GRCm38)	L588H	probably damaging	Het
Stk32b	T	A	5: 37,457,159 (GRCm38)	H335L	probably benign	Het
Taar4	A	C	10: 23,960,903 (GRCm38)	D137A	possibly damaging	Het
Tchh	CTCCGCCGGGAGCAAGAGCTCCGCCGGGAGCAAGAGTTCCGCCGGGAGCAAGAGCTCCGCCGGGAGCAAGAGTTCCGCCGGGAGCAAGAGCTCCGCC	CTCCGCCGGGAGCAAGAGCTCCGCCGGGAGCAAGAGTTCCGCCGGGAGCAAGAGCTCCGCC	3: 93,446,708 (GRCm38)		probably benign	Het
Tek	A	T	4: 94,849,837 (GRCm38)	H708L	probably benign	Het
Ugt2b38	T	C	5: 87,411,822 (GRCm38)	I404V	probably benign	Het
Vmn1r16	C	T	6: 57,322,829 (GRCm38)	M269I	probably benign	Het
Vmn1r201	A	G	13: 22,475,252 (GRCm38)	K212R	probably damaging	Het
Vmn2r11	A	C	5: 109,053,760 (GRCm38)	F293V	probably damaging	Het
Zdhhc17	A	T	10: 110,962,379 (GRCm38)		probably benign	Het
Zfp423	C	A	8: 87,782,710 (GRCm38)	M335I	probably benign	Het
Zfp74	T	C	7: 29,934,654 (GRCm38)	Y543C	probably damaging	Het
Zfp975	A	T	7: 42,662,840 (GRCm38)	S116R	probably benign	Het

Other mutations in Tmem89

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0060:Tmem89	UTSW	9	108,915,417 (GRCm38)	missense	probably damaging	1.00
R0060:Tmem89	UTSW	9	108,915,417 (GRCm38)	missense	probably damaging	1.00
R0063:Tmem89	UTSW	9	108,914,812 (GRCm38)	missense	probably benign	0.00
R0063:Tmem89	UTSW	9	108,914,812 (GRCm38)	missense	probably benign	0.00
R2447:Tmem89	UTSW	9	108,914,800 (GRCm38)	missense	probably damaging	0.98
R4778:Tmem89	UTSW	9	108,915,375 (GRCm38)	missense	probably damaging	0.98
R4906:Tmem89	UTSW	9	108,914,761 (GRCm38)	unclassified	probably benign
R5529:Tmem89	UTSW	9	108,915,477 (GRCm38)	missense	probably damaging	0.98
R9095:Tmem89	UTSW	9	108,914,661 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AGGAAATCGCATCTACCCCG -3'
(R):5'- CTTTCCAGTGTTTGCCGGAG -3'

Sequencing Primer
(F):5'- GTGGCTGGGGTCACAATCAC -3'
(R):5'- GGAATAGGTCACTGGGTCTCC -3'

Posted On

2021-03-08