Incidental Mutation 'R9313:Siglecg'
ID 705715
Institutional Source Beutler Lab
Gene Symbol Siglecg
Ensembl Gene ENSMUSG00000030468
Gene Name sialic acid binding Ig-like lectin G
Synonyms mSiglec-G, A630096C01Rik
MMRRC Submission
Accession Numbers
Essential gene? Probably non essential (E-score: 0.077) question?
Stock # R9313 (G1)
Quality Score 225.009
Status Not validated
Chromosome 7
Chromosomal Location 43408204-43418358 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 43412432 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 534 (D534G)
Ref Sequence ENSEMBL: ENSMUSP00000005592 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000005592]
AlphaFold Q80ZE3
Predicted Effect probably benign
Transcript: ENSMUST00000005592
AA Change: D534G

PolyPhen 2 Score 0.029 (Sensitivity: 0.95; Specificity: 0.82)
SMART Domains Protein: ENSMUSP00000005592
Gene: ENSMUSG00000030468
AA Change: D534G

DomainStartEndE-ValueType
signal peptide 1 17 N/A INTRINSIC
IG 27 139 5.21e-2 SMART
IG_like 148 232 8.97e0 SMART
IGc2 262 325 3.38e-10 SMART
IGc2 366 427 8.26e-5 SMART
low complexity region 473 480 N/A INTRINSIC
transmembrane domain 545 564 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002]
PHENOTYPE: Mice homozygous for a null allele exhibit increased B-1 cell numbers, increased IgM levels and IgM-producing plasma cells, and produce more IgM autoantibodies. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 66 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700006A11Rik T C 3: 124,413,571 T324A probably benign Het
4930452B06Rik T A 14: 8,518,635 T274S probably benign Het
Aadat G A 8: 60,526,601 V166I probably benign Het
Abca17 C T 17: 24,346,233 S75N probably benign Het
Adgrb1 A G 15: 74,539,775 T376A probably damaging Het
Atp5o CTTTGACG C 16: 91,926,916 probably null Het
Atp5o TTTGACGGT TT 16: 91,926,917 probably null Het
Car9 A G 4: 43,507,180 E42G probably benign Het
Cep41 T C 6: 30,680,346 K9R probably null Het
Ckm A G 7: 19,415,473 T141A probably benign Het
Clcc1 G T 3: 108,674,660 R360S probably benign Het
Clcn3 A C 8: 60,937,469 I146R probably damaging Het
Crlf1 A G 8: 70,498,816 D115G probably damaging Het
Dnah1 T A 14: 31,266,013 I3483F probably damaging Het
Dopey1 T C 9: 86,524,588 *386Q probably null Het
Eftud2 A G 11: 102,839,436 V899A probably benign Het
Ephx3 C G 17: 32,189,316 D45H probably benign Het
Faap100 A G 11: 120,376,862 S362P probably damaging Het
Fdps A T 3: 89,099,348 D78E probably benign Het
Fsd1l T G 4: 53,694,760 W405G probably damaging Het
Fsd1l T C 4: 53,701,093 V485A possibly damaging Het
Gm12887 C T 4: 121,616,504 V50M probably benign Het
Gm16486 G A 8: 70,708,704 G182D probably damaging Het
Gm45785 T C 7: 140,818,703 I94V unknown Het
Gm8251 A G 1: 44,057,360 V1526A probably benign Het
Hcls1 C T 16: 36,956,638 A230V probably benign Het
Hmcn1 A C 1: 150,646,592 V3519G probably benign Het
Hmgcs1 A G 13: 119,704,427 Y360C probably benign Het
Hsd17b13 A G 5: 103,965,773 probably null Het
Ift57 T A 16: 49,736,722 D235E possibly damaging Het
Iltifb A G 10: 118,294,233 C89R probably damaging Het
Krt9 T A 11: 100,188,721 Y615F unknown Het
Ltbp2 G A 12: 84,791,090 P1192L probably benign Het
Marco T C 1: 120,494,085 E130G probably damaging Het
Ms4a18 A T 19: 11,011,440 L184Q probably damaging Het
Mtus1 A T 8: 41,082,886 S598T probably damaging Het
Nbeal2 C A 9: 110,634,368 R1265L probably damaging Het
Odf2 G T 2: 29,926,803 G754C probably benign Het
Olfr1131 T C 2: 87,628,732 S90P probably benign Het
Olfr1274-ps C A 2: 90,401,573 T304K probably benign Het
Olfr164 T G 16: 19,286,350 Y131S probably benign Het
Olfr810 A T 10: 129,790,920 I223N probably damaging Het
Paqr8 G A 1: 20,934,904 W94* probably null Het
Pkd1 G A 17: 24,594,958 G4132D probably damaging Het
Pom121l2 A G 13: 21,984,336 M926V probably benign Het
Postn A T 3: 54,365,915 Y79F probably damaging Het
Prl7d1 T C 13: 27,709,199 E242G probably benign Het
Rcor3 G T 1: 192,125,881 H165Q possibly damaging Het
Rnf157 A G 11: 116,359,892 V161A probably damaging Het
Rnf38 G A 4: 44,143,584 T150M probably damaging Het
Scn4b T A 9: 45,146,715 V35E probably damaging Het
Serpina3i T C 12: 104,265,413 I103T probably damaging Het
Shcbp1 T A 8: 4,744,518 D425V probably damaging Het
Sidt2 T C 9: 45,941,360 T776A possibly damaging Het
Sipa1l3 T A 7: 29,378,014 T778S probably benign Het
Slc13a1 A G 6: 24,108,204 V291A probably benign Het
Stra6l A G 4: 45,881,454 I439V probably benign Het
Tdrd7 G A 4: 46,005,319 S375N probably benign Het
Vmn1r192 T A 13: 22,188,021 I10L probably benign Het
Vmn2r34 T G 7: 7,683,818 L293F possibly damaging Het
Xirp2 G T 2: 67,516,978 A3188S probably damaging Het
Zcchc6 A T 13: 59,799,984 M841K probably benign Het
Zfp219 A G 14: 52,008,743 V309A probably damaging Het
Zfp341 C A 2: 154,627,987 P197T probably damaging Het
Zfp628 G A 7: 4,919,550 R257H probably benign Het
Zfp644 T G 5: 106,636,458 Y741S probably benign Het
Other mutations in Siglecg
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00528:Siglecg APN 7 43409057 missense possibly damaging 0.64
IGL00556:Siglecg APN 7 43411795 missense probably benign 0.02
IGL01806:Siglecg APN 7 43411464 splice site probably null
IGL01947:Siglecg APN 7 43408763 missense probably benign 0.43
IGL02257:Siglecg APN 7 43411904 missense probably benign 0.00
IGL02410:Siglecg APN 7 43408829 missense probably damaging 0.99
IGL02454:Siglecg APN 7 43408895 missense probably benign 0.00
Chamonix UTSW 7 43409422 missense possibly damaging 0.91
Dollywood UTSW 7 43411099 missense probably damaging 1.00
glowworm UTSW 7 43408579 missense probably benign 0.04
Montblanc UTSW 7 43411386 intron probably benign
Shenandoah UTSW 7 43408802 missense probably damaging 0.99
shenandoah2 UTSW 7 43412017 missense possibly damaging 0.82
Sherando UTSW 7 43409057 missense possibly damaging 0.64
Smokies UTSW 7 43409279 missense probably benign 0.02
IGL02988:Siglecg UTSW 7 43418052 missense probably damaging 1.00
R0134:Siglecg UTSW 7 43411171 missense probably damaging 1.00
R0225:Siglecg UTSW 7 43411171 missense probably damaging 1.00
R0480:Siglecg UTSW 7 43411126 missense probably benign 0.42
R1538:Siglecg UTSW 7 43417889 missense possibly damaging 0.53
R1681:Siglecg UTSW 7 43408941 missense probably benign 0.17
R2358:Siglecg UTSW 7 43409422 missense possibly damaging 0.91
R4428:Siglecg UTSW 7 43417926 missense possibly damaging 0.84
R4429:Siglecg UTSW 7 43417926 missense possibly damaging 0.84
R4736:Siglecg UTSW 7 43417908 missense probably benign 0.03
R4754:Siglecg UTSW 7 43411871 intron probably benign
R5017:Siglecg UTSW 7 43411386 intron probably benign
R5713:Siglecg UTSW 7 43408802 missense probably damaging 0.99
R5777:Siglecg UTSW 7 43409413 missense possibly damaging 0.80
R5892:Siglecg UTSW 7 43412204 intron probably benign
R6153:Siglecg UTSW 7 43412017 missense possibly damaging 0.82
R6154:Siglecg UTSW 7 43412017 missense possibly damaging 0.82
R6331:Siglecg UTSW 7 43408754 missense possibly damaging 0.83
R6562:Siglecg UTSW 7 43409057 missense possibly damaging 0.64
R6749:Siglecg UTSW 7 43408979 missense probably benign 0.00
R7066:Siglecg UTSW 7 43411742 missense probably benign 0.40
R7884:Siglecg UTSW 7 43409279 missense probably benign 0.02
R8275:Siglecg UTSW 7 43412468 missense probably benign
R8554:Siglecg UTSW 7 43408896 missense probably benign 0.01
R8846:Siglecg UTSW 7 43412518 missense probably benign 0.02
R8873:Siglecg UTSW 7 43418024 missense probably benign 0.00
R8887:Siglecg UTSW 7 43408584 missense probably benign 0.18
R9012:Siglecg UTSW 7 43411099 missense probably damaging 1.00
R9032:Siglecg UTSW 7 43411625 missense probably benign 0.24
R9048:Siglecg UTSW 7 43408579 missense probably benign 0.04
R9085:Siglecg UTSW 7 43411625 missense probably benign 0.24
R9320:Siglecg UTSW 7 43409429 missense probably benign 0.33
R9745:Siglecg UTSW 7 43418052 missense probably damaging 0.98
RF006:Siglecg UTSW 7 43408864 nonsense probably null
Z1177:Siglecg UTSW 7 43412022 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ACTAAGATTCGCTATGGAGGGG -3'
(R):5'- TCGCCACCTTGCTAATACTG -3'

Sequencing Primer
(F):5'- CTCTGTGGTTAGAGCGAAGTC -3'
(R):5'- GCTAATACTGTTCCACCTGCC -3'
Posted On 2022-03-25