Mutagenetix > Incidental Mutation

Incidental Mutation 'R1546:Vcan'

172203

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, DPEAAE, heart defect, Cspg2, 5430420N07Rik

MMRRC Submission

039585-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1546 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

89655312-89742509 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 89692956 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 1490 (S1490P)

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109544

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109546
AA Change: S1490P

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: S1490P

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000159910
AA Change: S530P

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614
AA Change: S530P

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Coding Region Coverage

1x: 99.0%
3x: 98.0%
10x: 95.2%
20x: 88.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2510039O18Rik	T	C	4: 147,941,775	S251P	probably damaging	Het
4931409K22Rik	A	T	5: 24,555,428		probably null	Het
4932438A13Rik	T	C	3: 36,870,056	V10A	possibly damaging	Het
Aaas	C	A	15: 102,346,718	R79L	probably benign	Het
Acap2	C	A	16: 31,104,936	E657*	probably null	Het
Adgrg5	A	T	8: 94,941,630	E441V	probably benign	Het
Alkbh2	C	T	5: 114,124,226	E148K	probably damaging	Het
AY358078	C	T	14: 51,820,419		probably null	Het
Bco2	A	G	9: 50,550,629	V25A	possibly damaging	Het
Carf	T	A	1: 60,126,036		probably null	Het
Ccdc38	A	T	10: 93,565,879	I134L	probably benign	Het
Cgnl1	A	G	9: 71,725,815	S85P	probably benign	Het
Ctsl	A	G	13: 64,367,879	V126A	probably damaging	Het
Cwc27	A	C	13: 104,802,185	S206A	probably damaging	Het
D630045J12Rik	A	G	6: 38,190,655	I1004T	probably damaging	Het
Dgki	A	G	6: 37,050,203	V401A	probably damaging	Het
Dpp8	C	T	9: 65,063,493	H545Y	possibly damaging	Het
Dpy19l1	A	T	9: 24,475,384	C205S	probably damaging	Het
Enpp2	C	T	15: 54,845,829	E797K	probably benign	Het
Ephb2	C	T	4: 136,771,009	R253H	probably damaging	Het
Esrra	T	C	19: 6,920,297	T31A	probably benign	Het
Ewsr1	C	A	11: 5,078,574		probably benign	Het
Flt4	G	T	11: 49,631,981	R475L	probably benign	Het
Gm13547	A	G	2: 29,763,909	E138G	possibly damaging	Het
Gm572	A	T	4: 148,666,819	R216S	possibly damaging	Het
Hapln2	T	A	3: 88,024,097	Y37F	probably benign	Het
Hcn1	ACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC	ACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC	13: 117,975,766		probably benign	Het
Hhla1	C	T	15: 65,933,327	A369T	probably benign	Het
Hist1h2ae	A	G	13: 23,570,945	V55A	probably damaging	Het
Hmg20a	T	A	9: 56,467,401	F14I	possibly damaging	Het
Itga2	A	T	13: 114,849,420	S940T	possibly damaging	Het
Kcnt2	A	G	1: 140,431,378	N377S	probably benign	Het
Kirrel	C	T	3: 87,089,151	M380I	probably null	Het
Lhx6	A	G	2: 36,091,037	S298P	probably benign	Het
Lrp2	C	T	2: 69,502,610	G1521D	probably damaging	Het
Mogat2	A	G	7: 99,232,559	W57R	probably damaging	Het
Ms4a3	T	C	19: 11,632,907	N97S	probably benign	Het
Myo1a	A	G	10: 127,712,624	D380G	probably damaging	Het
Nufip2	T	A	11: 77,691,606	D115E	probably damaging	Het
Ogn	A	T	13: 49,609,333	K50N	probably benign	Het
Olfr202	T	C	16: 59,284,003	R165G	probably damaging	Het
Olfr250	A	T	9: 38,367,548	M1L	probably benign	Het
Pde8b	G	A	13: 95,046,443	T269I	probably damaging	Het
Ppargc1b	A	T	18: 61,310,606	D495E	probably damaging	Het
Prdm16	C	A	4: 154,528,660	K103N	possibly damaging	Het
Proc	C	T	18: 32,127,410	G221S	probably damaging	Het
Pxk	A	G	14: 8,164,091	N561S	probably damaging	Het
Rapgef5	A	G	12: 117,647,101	N323S	probably benign	Het
Slc6a13	T	G	6: 121,332,374	D281E	possibly damaging	Het
Slc8a1	T	C	17: 81,648,247	Y454C	probably damaging	Het
Sntg2	C	A	12: 30,288,296	L115F	probably damaging	Het
Spata13	A	G	14: 60,756,408	D1103G	probably damaging	Het
Supv3l1	G	A	10: 62,432,446	A540V	probably benign	Het
Tet1	A	T	10: 62,812,910	D1914E	probably damaging	Het
Tmem30a	A	G	9: 79,771,288	*329Q	probably null	Het
Tspan5	A	T	3: 138,898,341	L162F	probably damaging	Het
Ttn	T	A	2: 76,719,052	K31760N	probably damaging	Het
Tyr	A	T	7: 87,437,992	D437E	probably benign	Het
Ubr4	T	A	4: 139,416,927	L1427*	probably null	Het
Utrn	C	A	10: 12,436,364	D616Y	probably damaging	Het
Vcl	T	A	14: 21,008,950	C545S	probably damaging	Het
Vmn2r4	C	T	3: 64,406,888	G224D	probably damaging	Het
Vmn2r97	T	A	17: 18,947,848	V788E	probably damaging	Het
Vrtn	G	A	12: 84,648,508	V11M	probably damaging	Het
Zbtb21	A	T	16: 97,952,027	V380D	probably damaging	Het
Zcchc14	G	A	8: 121,604,263		probably benign	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89704702	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89692319	missense	probably benign
IGL00504:Vcan	APN	13	89691275	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89688979	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89703726	missense	probably benign
IGL00743:Vcan	APN	13	89725306	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89662052	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89679958	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89691668	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89703943	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89704169	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89688675	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89690586	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89689745	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89725388	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89689205	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89689359	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89684438	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89725565	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89684493	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89703077	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89704849	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89690657	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89688623	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89703275	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89661932	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89705006	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89661985	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89703668	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89703668	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89678073	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89691261	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89712191	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89691275	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89703546	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89703199	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89678145	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89704660	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89703772	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89712253	missense	probably damaging	1.00
R0561:Vcan	UTSW	13	89731464	missense	possibly damaging	0.86
R0636:Vcan	UTSW	13	89704706	missense	probably damaging	0.99
R0636:Vcan	UTSW	13	89712267	missense	probably damaging	1.00
R0680:Vcan	UTSW	13	89679822	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89704953	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89685077	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89692410	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89705663	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89704303	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89679794	splice site	probably null
R1219:Vcan	UTSW	13	89679904	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89657556	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89693055	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89693055	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89688484	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89688484	missense	probably benign	0.00
R1604:Vcan	UTSW	13	89689661	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89705663	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89703667	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89661946	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89703547	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89688483	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89721775	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89704735	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89691681	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89705212	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89705392	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89690871	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89693015	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89705534	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89702926	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89703424	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89692742	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89689038	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89690985	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89693303	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89693303	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89693303	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89689737	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89703529	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89703410	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89689449	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89704675	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89704237	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89703301	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89692543	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89692543	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89692543	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89679887	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89685158	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89725547	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89725486	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89704028	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89679934	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89705789	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89704739	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89688842	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89703977	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89657472	intron	probably benign
R5124:Vcan	UTSW	13	89725517	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89690240	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89690872	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89692532	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89691600	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89690286	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89691810	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89703112	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89688671	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89691503	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89691135	frame shift	probably null
R5687:Vcan	UTSW	13	89678134	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89679950	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89703952	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89692691	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89657536	nonsense	probably null
R6135:Vcan	UTSW	13	89689926	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89691220	nonsense	probably null
R6280:Vcan	UTSW	13	89725373	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89691597	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89704832	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89690687	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89704731	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89705182	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89705125	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89712381	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89689431	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89678133	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89679956	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89693407	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89690618	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89690241	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89705686	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89689110	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89725591	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89688936	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89705270	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89721686	missense	probably damaging	1.00
R7250:Vcan	UTSW	13	89731457	critical splice donor site	probably null
R7262:Vcan	UTSW	13	89705161	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89692733	nonsense	probably null
R7299:Vcan	UTSW	13	89705266	missense	probably benign
R7301:Vcan	UTSW	13	89705266	missense	probably benign
R7425:Vcan	UTSW	13	89689832	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89704118	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89692458	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89692223	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89685114	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89685114	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89691789	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89704619	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89689323	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89692937	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89688654	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89688654	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89691858	missense	probably benign
R7998:Vcan	UTSW	13	89704327	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89704360	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89657290	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89657658	missense	probably damaging	1.00
R8103:Vcan	UTSW	13	89703320	nonsense	probably null
R8124:Vcan	UTSW	13	89704254	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89704987	nonsense	probably null
R8166:Vcan	UTSW	13	89692736	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89704970	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89704335	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89688743	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89691098	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89692320	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89692111	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89704907	missense	probably benign
R9049:Vcan	UTSW	13	89678105	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89691027	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89704525	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89679931	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89690496	nonsense	probably null
R9259:Vcan	UTSW	13	89690870	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89689333	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89703412	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89693009	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89691540	missense
R9579:Vcan	UTSW	13	89689594	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89705372	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89692962	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89691741	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89691128	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89689811	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89692493	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89705749	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89692571	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89703524	missense	probably benign	0.00
Z1177:Vcan	UTSW	13	89703788	nonsense	probably null
Z1177:Vcan	UTSW	13	89704073	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGAGCTGAAGAACTCTCCGAGAAC -3'
(R):5'- TCGTTACCACAGTGCCTAAGGACC -3'

Sequencing Primer
(F):5'- GAGTAAGTGGTTCTTCCTCCAAGAC -3'
(R):5'- CAGTACGAAAGTGTTGCACCTTC -3'

Posted On

2014-04-13