Mutagenetix > Incidental Mutation

Incidental Mutation 'R1852:Vcan'

208250

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, heart defect, Cspg2, DPEAAE, 5430420N07Rik

MMRRC Submission

039876-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1852 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

89803431-89890628 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 89853511 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Alanine at position 483 (E483A)

Ref Sequence

ENSEMBL: ENSMUSP00000105171 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109544
AA Change: E483A

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614
AA Change: E483A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109546
AA Change: E483A

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: E483A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159910

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.0%
20x: 91.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 106 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900092C05Rik	A	G	7: 12,246,629 (GRCm39)		probably null	Het
4930433I11Rik	T	A	7: 40,643,037 (GRCm39)	D326E	probably benign	Het
Abca8a	T	C	11: 109,960,212 (GRCm39)	D676G	probably damaging	Het
Abcb7	T	C	X: 103,349,005 (GRCm39)	M153V	probably benign	Het
Ablim3	G	A	18: 61,982,466 (GRCm39)	H160Y	probably benign	Het
Acsl6	T	C	11: 54,251,902 (GRCm39)	I683T	probably damaging	Het
Adgrg5	A	T	8: 95,664,428 (GRCm39)	Y345F	probably damaging	Het
Alg6	T	G	4: 99,634,599 (GRCm39)	F114V	probably benign	Het
Ank2	A	C	3: 126,791,500 (GRCm39)		probably null	Het
Ankrd24	A	T	10: 81,478,775 (GRCm39)		probably benign	Het
Ano8	T	C	8: 71,936,131 (GRCm39)	K222E	probably damaging	Het
Arhgef5	T	C	6: 43,252,119 (GRCm39)	S957P	probably benign	Het
Bltp2	A	G	11: 78,159,299 (GRCm39)	D499G	probably damaging	Het
Bmpr1b	A	T	3: 141,563,163 (GRCm39)		probably null	Het
C3	A	G	17: 57,529,823 (GRCm39)	V2A	probably damaging	Het
Capn1	A	G	19: 6,059,133 (GRCm39)	V276A	possibly damaging	Het
Ccdc57	T	C	11: 120,812,499 (GRCm39)	E85G	probably damaging	Het
Chil3	C	T	3: 106,056,117 (GRCm39)		probably null	Het
Chl1	A	T	6: 103,676,120 (GRCm39)		probably null	Het
Chodl	C	T	16: 78,738,146 (GRCm39)	P38L	probably benign	Het
Clec4a2	G	T	6: 123,116,084 (GRCm39)	E124*	probably null	Het
Cmtr1	T	C	17: 29,921,229 (GRCm39)	V825A	probably benign	Het
Cnksr3	T	C	10: 7,070,539 (GRCm39)	I232V	probably benign	Het
Cntn1	C	T	15: 92,203,021 (GRCm39)	R768C	probably damaging	Het
Col15a1	G	T	4: 47,299,278 (GRCm39)		probably null	Het
Col6a3	T	A	1: 90,735,256 (GRCm39)	I798F	possibly damaging	Het
Cpxcr1	T	A	X: 115,387,758 (GRCm39)	L223*	probably null	Het
Cul1	A	G	6: 47,497,764 (GRCm39)	N615S	probably damaging	Het
Cxcr2	A	G	1: 74,198,438 (GRCm39)	I311V	probably benign	Het
Cyp2c67	C	T	19: 39,605,811 (GRCm39)	G362S	probably benign	Het
D930048N14Rik	T	A	11: 51,544,692 (GRCm39)		probably benign	Het
Ddx49	T	C	8: 70,753,633 (GRCm39)	T79A	probably damaging	Het
Defb46	T	A	8: 19,289,991 (GRCm39)		probably null	Het
Dnah17	T	A	11: 118,012,742 (GRCm39)	I145F	probably damaging	Het
Dock8	A	T	19: 25,104,492 (GRCm39)	I725F	probably benign	Het
Dppa4	G	A	16: 48,108,247 (GRCm39)	A11T	probably damaging	Het
Enam	T	A	5: 88,652,324 (GRCm39)	S1278T	possibly damaging	Het
Fndc3a	A	G	14: 72,794,283 (GRCm39)	V829A	probably damaging	Het
Gatb	T	G	3: 85,526,184 (GRCm39)	L354R	probably damaging	Het
Gm10787	A	T	10: 76,857,711 (GRCm39)		noncoding transcript	Het
Gsap	T	A	5: 21,495,543 (GRCm39)		probably null	Het
Gtf2ird1	T	C	5: 134,411,434 (GRCm39)		probably null	Het
Has3	A	T	8: 107,600,711 (GRCm39)	I58F	probably damaging	Het
Haus5	A	T	7: 30,357,926 (GRCm39)		probably null	Het
Hip1r	T	C	5: 124,129,568 (GRCm39)	V169A	probably benign	Het
Hnf1a	T	A	5: 115,108,770 (GRCm39)	D45V	probably damaging	Het
Il20ra	T	A	10: 19,618,767 (GRCm39)	Y72N	probably damaging	Het
Ipo11	A	T	13: 106,948,765 (GRCm39)	V914E	possibly damaging	Het
Itpr1	T	C	6: 108,363,667 (GRCm39)	L763P	probably damaging	Het
Katnal2	C	A	18: 77,103,719 (GRCm39)	R104L	probably benign	Het
Kcng4	G	T	8: 120,352,947 (GRCm39)	P321Q	probably benign	Het
Larp4b	T	C	13: 9,187,339 (GRCm39)		probably null	Het
Lars1	T	C	18: 42,345,673 (GRCm39)	N1001S	probably benign	Het
Lhcgr	T	A	17: 89,072,604 (GRCm39)	I148F	probably damaging	Het
Lsm10	T	A	4: 125,991,756 (GRCm39)	S37R	possibly damaging	Het
Mfsd13a	A	G	19: 46,360,619 (GRCm39)		probably null	Het
Mipep	T	A	14: 61,080,689 (GRCm39)	C560*	probably null	Het
Mme	G	A	3: 63,235,467 (GRCm39)	D172N	probably benign	Het
Mme	A	G	3: 63,235,404 (GRCm39)	S97G	probably benign	Het
Mtrex	A	T	13: 113,009,461 (GRCm39)	H979Q	probably benign	Het
Mx1	A	T	16: 97,249,403 (GRCm39)	L608Q	probably damaging	Het
Myt1l	T	C	12: 29,901,660 (GRCm39)	M208T	probably benign	Het
Neb	T	C	2: 52,118,554 (GRCm39)	I3987V	probably damaging	Het
Nelfcd	A	G	2: 174,265,771 (GRCm39)		probably null	Het
Neto1	T	C	18: 86,414,009 (GRCm39)	M1T	probably null	Het
Nom1	T	C	5: 29,651,876 (GRCm39)	F738S	possibly damaging	Het
Or10v1	C	A	19: 11,874,249 (GRCm39)	P288H	probably damaging	Het
Or4f61	C	A	2: 111,922,192 (GRCm39)	V285L	probably benign	Het
Or51h5	C	T	7: 102,577,648 (GRCm39)	S271L	probably damaging	Het
Or5b123	C	T	19: 13,596,967 (GRCm39)	S147F	probably damaging	Het
Or5w14	T	C	2: 87,541,317 (GRCm39)		probably null	Het
Or6c203	T	C	10: 129,010,197 (GRCm39)	K231R	probably benign	Het
Or6f2	T	A	7: 139,756,474 (GRCm39)	L147*	probably null	Het
Or9m1b	T	G	2: 87,836,865 (GRCm39)	I86L	probably damaging	Het
Pak1ip1	A	G	13: 41,164,708 (GRCm39)	T264A	possibly damaging	Het
Palb2	G	T	7: 121,713,537 (GRCm39)	D915E	possibly damaging	Het
Pank4	T	C	4: 155,060,816 (GRCm39)	L491P	probably damaging	Het
Paqr7	T	C	4: 134,234,980 (GRCm39)	V279A	probably benign	Het
Pcif1	G	A	2: 164,730,386 (GRCm39)	R373Q	probably damaging	Het
Pdgfa	T	A	5: 138,964,927 (GRCm39)	N185I	probably benign	Het
Plcd4	T	C	1: 74,588,520 (GRCm39)	V123A	possibly damaging	Het
Pramel26	T	C	4: 143,539,396 (GRCm39)	I32M	probably benign	Het
Prune2	A	G	19: 17,176,503 (GRCm39)	I154V	probably damaging	Het
Rac3	T	G	11: 120,614,320 (GRCm39)	L148R	possibly damaging	Het
Rapgef6	T	A	11: 54,533,637 (GRCm39)	D362E	probably benign	Het
Rbl1	A	G	2: 157,016,823 (GRCm39)	I592T	probably benign	Het
Rbl2	A	C	8: 91,822,191 (GRCm39)	D408A	possibly damaging	Het
Retreg2	A	G	1: 75,123,319 (GRCm39)	K416E	probably benign	Het
Rorb	A	T	19: 18,939,447 (GRCm39)	L234H	probably damaging	Het
Rpp40	T	G	13: 36,080,897 (GRCm39)	D279A	probably benign	Het
Siglec1	C	A	2: 130,923,420 (GRCm39)	V442L	probably damaging	Het
Sik3	C	G	9: 46,132,387 (GRCm39)	H1276Q	probably benign	Het
Slc25a34	G	A	4: 141,349,579 (GRCm39)	T192I	probably benign	Het
Sp140l1	G	A	1: 85,062,852 (GRCm39)		probably benign	Het
Sptbn5	T	C	2: 119,902,125 (GRCm39)	I126M	possibly damaging	Het
Strada	A	G	11: 106,062,047 (GRCm39)	V94A	possibly damaging	Het
Stxbp5	C	A	10: 9,688,042 (GRCm39)	V420F	possibly damaging	Het
Tgm1	T	C	14: 55,942,398 (GRCm39)	Y651C	probably damaging	Het
Tmem200c	T	A	17: 69,147,612 (GRCm39)	V65E	probably damaging	Het
Tmem74	A	T	15: 43,730,559 (GRCm39)	D161E	probably benign	Het
Trgc3	A	T	13: 19,445,261 (GRCm39)	M70L	probably damaging	Het
Vmn2r124	A	G	17: 18,283,436 (GRCm39)	T377A	probably benign	Het
Vmn2r3	A	C	3: 64,166,815 (GRCm39)	I772S	probably damaging	Het
Wdfy3	A	G	5: 102,063,242 (GRCm39)	V1342A	probably benign	Het
Zfp507	T	C	7: 35,487,176 (GRCm39)	H764R	probably damaging	Het
Znrf3	A	G	11: 5,237,455 (GRCm39)	I218T	possibly damaging	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89,852,821 (GRCm39)	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89,840,438 (GRCm39)	missense	probably benign
IGL00504:Vcan	APN	13	89,839,394 (GRCm39)	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89,837,098 (GRCm39)	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89,851,845 (GRCm39)	missense	probably benign
IGL00743:Vcan	APN	13	89,873,425 (GRCm39)	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89,810,171 (GRCm39)	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89,828,077 (GRCm39)	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89,839,787 (GRCm39)	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89,852,062 (GRCm39)	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89,852,288 (GRCm39)	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89,836,794 (GRCm39)	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89,838,705 (GRCm39)	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89,837,864 (GRCm39)	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89,873,507 (GRCm39)	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89,837,324 (GRCm39)	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89,837,478 (GRCm39)	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89,832,557 (GRCm39)	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89,873,684 (GRCm39)	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89,832,612 (GRCm39)	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89,851,196 (GRCm39)	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89,852,968 (GRCm39)	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89,838,776 (GRCm39)	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89,836,742 (GRCm39)	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89,851,394 (GRCm39)	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89,810,051 (GRCm39)	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89,853,125 (GRCm39)	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89,810,104 (GRCm39)	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89,826,192 (GRCm39)	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89,839,380 (GRCm39)	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89,860,310 (GRCm39)	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89,839,394 (GRCm39)	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89,851,665 (GRCm39)	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89,851,318 (GRCm39)	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89,826,264 (GRCm39)	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89,852,779 (GRCm39)	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89,851,891 (GRCm39)	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89,879,583 (GRCm39)	missense	possibly damaging	0.86
R0561:Vcan	UTSW	13	89,860,372 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,860,386 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,852,825 (GRCm39)	missense	probably damaging	0.99
R0680:Vcan	UTSW	13	89,827,941 (GRCm39)	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89,853,072 (GRCm39)	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89,833,196 (GRCm39)	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89,840,529 (GRCm39)	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89,852,422 (GRCm39)	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89,827,913 (GRCm39)	splice site	probably null
R1219:Vcan	UTSW	13	89,828,023 (GRCm39)	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89,805,675 (GRCm39)	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89,841,075 (GRCm39)	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89,837,780 (GRCm39)	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89,851,786 (GRCm39)	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89,810,065 (GRCm39)	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89,851,666 (GRCm39)	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89,836,602 (GRCm39)	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89,869,894 (GRCm39)	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89,852,854 (GRCm39)	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89,839,800 (GRCm39)	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89,853,331 (GRCm39)	missense	possibly damaging	0.75
R1868:Vcan	UTSW	13	89,838,990 (GRCm39)	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89,841,134 (GRCm39)	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89,853,653 (GRCm39)	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89,851,045 (GRCm39)	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89,851,543 (GRCm39)	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89,840,861 (GRCm39)	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89,837,157 (GRCm39)	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89,839,104 (GRCm39)	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89,837,856 (GRCm39)	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89,851,648 (GRCm39)	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89,851,529 (GRCm39)	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89,837,568 (GRCm39)	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89,852,794 (GRCm39)	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89,852,356 (GRCm39)	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89,851,420 (GRCm39)	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89,828,006 (GRCm39)	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89,833,277 (GRCm39)	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89,873,666 (GRCm39)	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89,873,605 (GRCm39)	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89,852,147 (GRCm39)	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89,828,053 (GRCm39)	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89,853,908 (GRCm39)	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89,852,858 (GRCm39)	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89,836,961 (GRCm39)	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89,852,096 (GRCm39)	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89,805,591 (GRCm39)	intron	probably benign
R5124:Vcan	UTSW	13	89,873,636 (GRCm39)	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89,838,359 (GRCm39)	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89,838,991 (GRCm39)	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89,840,651 (GRCm39)	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89,839,719 (GRCm39)	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89,838,405 (GRCm39)	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89,839,929 (GRCm39)	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89,851,231 (GRCm39)	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89,836,790 (GRCm39)	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89,839,622 (GRCm39)	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89,839,254 (GRCm39)	frame shift	probably null
R5687:Vcan	UTSW	13	89,826,253 (GRCm39)	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89,828,069 (GRCm39)	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89,852,071 (GRCm39)	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89,840,810 (GRCm39)	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89,805,655 (GRCm39)	nonsense	probably null
R6135:Vcan	UTSW	13	89,838,045 (GRCm39)	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89,839,339 (GRCm39)	nonsense	probably null
R6280:Vcan	UTSW	13	89,873,492 (GRCm39)	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89,839,716 (GRCm39)	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89,852,951 (GRCm39)	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89,838,806 (GRCm39)	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89,852,850 (GRCm39)	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89,853,301 (GRCm39)	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89,853,244 (GRCm39)	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89,860,500 (GRCm39)	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89,837,550 (GRCm39)	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89,826,252 (GRCm39)	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89,828,075 (GRCm39)	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89,841,526 (GRCm39)	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89,838,737 (GRCm39)	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89,838,360 (GRCm39)	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89,853,805 (GRCm39)	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89,837,229 (GRCm39)	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89,873,710 (GRCm39)	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89,837,055 (GRCm39)	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89,853,389 (GRCm39)	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89,879,576 (GRCm39)	critical splice donor site	probably null
R7250:Vcan	UTSW	13	89,869,805 (GRCm39)	missense	probably damaging	1.00
R7262:Vcan	UTSW	13	89,853,280 (GRCm39)	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89,840,852 (GRCm39)	nonsense	probably null
R7299:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7301:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7425:Vcan	UTSW	13	89,837,951 (GRCm39)	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89,852,237 (GRCm39)	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89,840,577 (GRCm39)	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89,840,342 (GRCm39)	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89,839,908 (GRCm39)	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89,852,738 (GRCm39)	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89,837,442 (GRCm39)	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89,841,056 (GRCm39)	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89,839,977 (GRCm39)	missense	probably benign
R7998:Vcan	UTSW	13	89,852,446 (GRCm39)	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89,852,479 (GRCm39)	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89,805,409 (GRCm39)	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89,851,439 (GRCm39)	nonsense	probably null
R8103:Vcan	UTSW	13	89,805,777 (GRCm39)	missense	probably damaging	1.00
R8124:Vcan	UTSW	13	89,852,373 (GRCm39)	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89,853,106 (GRCm39)	nonsense	probably null
R8166:Vcan	UTSW	13	89,840,855 (GRCm39)	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89,853,089 (GRCm39)	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89,852,454 (GRCm39)	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89,836,862 (GRCm39)	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89,839,217 (GRCm39)	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89,840,439 (GRCm39)	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89,840,230 (GRCm39)	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89,853,026 (GRCm39)	missense	probably benign
R9049:Vcan	UTSW	13	89,826,224 (GRCm39)	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89,839,146 (GRCm39)	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89,852,644 (GRCm39)	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89,828,050 (GRCm39)	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89,838,615 (GRCm39)	nonsense	probably null
R9259:Vcan	UTSW	13	89,838,989 (GRCm39)	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89,837,452 (GRCm39)	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89,851,531 (GRCm39)	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89,841,128 (GRCm39)	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89,839,659 (GRCm39)	missense
R9579:Vcan	UTSW	13	89,837,713 (GRCm39)	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89,853,491 (GRCm39)	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89,841,081 (GRCm39)	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89,839,860 (GRCm39)	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89,839,247 (GRCm39)	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89,837,930 (GRCm39)	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89,840,612 (GRCm39)	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89,853,868 (GRCm39)	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89,840,690 (GRCm39)	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89,852,192 (GRCm39)	missense	probably damaging	1.00
Z1177:Vcan	UTSW	13	89,851,907 (GRCm39)	nonsense	probably null
Z1177:Vcan	UTSW	13	89,851,643 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TCCATAGTGACTTGTGGTTGCC -3'
(R):5'- CAGATTAGCCATGGAATCACCC -3'

Sequencing Primer
(F):5'- AATTCAGGACTGGCGCTTAC -3'
(R):5'- TGGAATCACCCACAACTACTCGG -3'

Posted On

2014-06-23