Mutagenetix > Incidental Mutation

Incidental Mutation 'R2207:Tg'

239179

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000053469

Gene Name

thyroglobulin

Synonyms

Tgn

MMRRC Submission

040209-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R2207 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66670753-66850721 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 66681939 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 401 (G401D)

Ref Sequence

ENSEMBL: ENSMUSP00000070239 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065916]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000065916
AA Change: G401D

PolyPhen 2 Score 0.150 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469
AA Change: G401D

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.5%
20x: 95.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mice homozygous for a spontaneous mutation exhibit enlarged thyroid gland, hypothyroidism, abnormal thyroid gland morphology, and decreased body weight. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 99 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700014D04Rik	A	G	13: 59,743,106 (GRCm38)	V300A	probably benign	Het
2410137M14Rik	A	G	17: 36,978,073 (GRCm38)		probably benign	Het
8430408G22Rik	G	A	6: 116,651,722 (GRCm38)	V9M	possibly damaging	Het
Abcb1b	C	T	5: 8,824,803 (GRCm38)	R488C	probably benign	Het
Adam34	T	C	8: 43,652,237 (GRCm38)	I124V	probably benign	Het
Aicda	T	A	6: 122,561,285 (GRCm38)	V134D	possibly damaging	Het
Akt2	T	A	7: 27,637,200 (GRCm38)		probably null	Het
Aldh1a3	T	C	7: 66,406,021 (GRCm38)	R341G	probably damaging	Het
Ankrd12	G	T	17: 66,031,574 (GRCm38)		probably null	Het
Anxa11	A	G	14: 25,874,297 (GRCm38)	Y244C	probably damaging	Het
Atp2c2	G	A	8: 119,748,309 (GRCm38)	R551Q	probably damaging	Het
B4galt3	C	A	1: 171,274,043 (GRCm38)	H196N	probably damaging	Het
Bcl11a	G	T	11: 24,163,343 (GRCm38)	G229W	probably damaging	Het
Brip1	A	G	11: 86,061,877 (GRCm38)	V1026A	probably benign	Het
Cacna1h	A	T	17: 25,385,013 (GRCm38)	S1282T	probably benign	Het
Calcr	T	C	6: 3,717,133 (GRCm38)	Y109C	probably damaging	Het
Ccdc39	T	C	3: 33,836,733 (GRCm38)	I241V	probably damaging	Het
Ccdc9	A	G	7: 16,284,269 (GRCm38)		probably benign	Het
Cdk17	G	A	10: 93,228,762 (GRCm38)	D298N	probably damaging	Het
Cdkl3	A	G	11: 52,027,193 (GRCm38)	*354W	probably null	Het
Celf6	A	T	9: 59,604,327 (GRCm38)	Y401F	possibly damaging	Het
Clec4a4	C	T	6: 123,013,807 (GRCm38)	L169F	probably damaging	Het
Col14a1	T	C	15: 55,463,686 (GRCm38)	F1411L	unknown	Het
Col4a2	G	T	8: 11,443,352 (GRCm38)	G1354W	probably damaging	Het
Crygs	C	T	16: 22,805,551 (GRCm38)	G102D	possibly damaging	Het
Cst13	A	T	2: 148,823,282 (GRCm38)	R66W	probably damaging	Het
Dhx8	A	G	11: 101,750,971 (GRCm38)	T632A	probably benign	Het
Disp1	A	G	1: 183,088,342 (GRCm38)	F838S	possibly damaging	Het
Dlg1	A	G	16: 31,853,846 (GRCm38)	H599R	probably benign	Het
Dnah12	T	A	14: 26,781,787 (GRCm38)	V1654E	probably damaging	Het
Dnah5	C	T	15: 28,343,671 (GRCm38)	L2406F	probably benign	Het
Fam98b	C	G	2: 117,267,819 (GRCm38)	R257G	probably damaging	Het
Fbn2	A	T	18: 58,081,399 (GRCm38)	C900*	probably null	Het
Fgf1	A	G	18: 38,847,085 (GRCm38)	Y79H	possibly damaging	Het
Foxn1	C	A	11: 78,358,804 (GRCm38)	A632S	probably benign	Het
Fsip2	T	G	2: 82,977,479 (GRCm38)	S1381A	probably benign	Het
Gtf2b	G	A	3: 142,778,320 (GRCm38)	G85D	probably benign	Het
Gyg	C	A	3: 20,150,539 (GRCm38)	G161C	probably damaging	Het
Hemgn	G	T	4: 46,396,301 (GRCm38)	L312I	possibly damaging	Het
Hic2	T	C	16: 17,257,460 (GRCm38)	M51T	possibly damaging	Het
Hivep2	C	A	10: 14,128,969 (GRCm38)	T437K	probably benign	Het
Hpx	C	T	7: 105,592,426 (GRCm38)	R287H	probably damaging	Het
Igf2bp3	C	T	6: 49,088,554 (GRCm38)	G468E	possibly damaging	Het
Il5ra	C	A	6: 106,712,441 (GRCm38)	E397*	probably null	Het
Itfg1	A	T	8: 85,776,198 (GRCm38)	S246R	probably benign	Het
Kdm2a	A	T	19: 4,362,870 (GRCm38)	D29E	probably damaging	Het
Lamc2	T	G	1: 153,133,706 (GRCm38)	E784D	possibly damaging	Het
Lrp2	A	T	2: 69,467,028 (GRCm38)	N3196K	possibly damaging	Het
Lyzl1	T	A	18: 4,181,962 (GRCm38)	C96*	probably null	Het
Maf1	A	G	15: 76,352,518 (GRCm38)	T17A	probably benign	Het
Map1b	T	C	13: 99,431,083 (GRCm38)	D1710G	unknown	Het
Megf8	C	T	7: 25,349,797 (GRCm38)	T1773I	probably damaging	Het
Mei1	T	C	15: 82,103,249 (GRCm38)	M414T	probably benign	Het
Myo15	A	G	11: 60,506,034 (GRCm38)	N2643S	probably benign	Het
Ndufa9	A	G	6: 126,844,809 (GRCm38)	Y64H	probably damaging	Het
Neb	A	T	2: 52,211,567 (GRCm38)	L4354*	probably null	Het
Nom1	T	A	5: 29,439,974 (GRCm38)	I480N	probably damaging	Het
Nrxn3	C	T	12: 89,348,312 (GRCm38)	T331M	probably damaging	Het
Olfr1307	A	C	2: 111,944,925 (GRCm38)	F177C	probably damaging	Het
Olfr345	G	T	2: 36,640,189 (GRCm38)	R50M	possibly damaging	Het
Olfr403	G	T	11: 74,196,324 (GRCm38)	V274L	possibly damaging	Het
Olfr643	A	G	7: 104,059,405 (GRCm38)	S66P	probably damaging	Het
Pcdhb15	A	T	18: 37,475,022 (GRCm38)	T436S	probably benign	Het
Pcdhb18	T	A	18: 37,491,289 (GRCm38)	N557K	probably damaging	Het
Pcdhb6	T	C	18: 37,335,580 (GRCm38)	M518T	probably benign	Het
Pgm2l1	T	A	7: 100,268,112 (GRCm38)		probably null	Het
Pih1d2	C	A	9: 50,621,079 (GRCm38)	H162N	probably benign	Het
Pitrm1	T	A	13: 6,569,291 (GRCm38)	Y721N	probably damaging	Het
Pla2r1	C	A	2: 60,458,435 (GRCm38)	V618F	probably damaging	Het
Plb1	T	C	5: 32,316,640 (GRCm38)	S599P	possibly damaging	Het
Prkg1	T	C	19: 30,578,860 (GRCm38)	D562G	probably damaging	Het
Proser1	T	G	3: 53,478,391 (GRCm38)	S565A	probably benign	Het
Prx	T	A	7: 27,516,788 (GRCm38)	V238E	probably damaging	Het
Psapl1	T	C	5: 36,205,165 (GRCm38)	I367T	probably damaging	Het
Rc3h1	T	C	1: 160,940,025 (GRCm38)	V128A	probably damaging	Het
Rrm1	T	A	7: 102,442,026 (GRCm38)	M1K	probably null	Het
Rsf1	GCG	GCGACG	7: 97,579,907 (GRCm38)		probably benign	Het
Rsl1	A	G	13: 67,182,828 (GRCm38)	T447A	probably benign	Het
Ryr2	A	G	13: 11,810,937 (GRCm38)	S552P	probably damaging	Het
Sbf1	A	G	15: 89,306,693 (GRCm38)	S225P	possibly damaging	Het
Serpina3c	T	A	12: 104,151,498 (GRCm38)	I194F	probably benign	Het
Setd3	C	T	12: 108,107,285 (GRCm38)	V578M	probably benign	Het
Setx	GTGGCT	GT	2: 29,154,061 (GRCm38)	1814	probably null	Het
Slc6a1	T	A	6: 114,308,671 (GRCm38)	V356E	probably damaging	Het
Slfn1	A	G	11: 83,121,166 (GRCm38)	E36G	possibly damaging	Het
Sorcs1	T	C	19: 50,230,217 (GRCm38)	H609R	possibly damaging	Het
Spag16	A	G	1: 70,724,884 (GRCm38)	H621R	probably benign	Het
Tnxb	A	C	17: 34,709,417 (GRCm38)	T2602P	possibly damaging	Het
Trip11	T	C	12: 101,873,442 (GRCm38)	N1643S	probably benign	Het
Ttn	G	A	2: 76,879,343 (GRCm38)	R1581*	probably null	Het
Ttn	A	T	2: 76,965,811 (GRCm38)	I590K	probably benign	Het
Vmn1r225	A	G	17: 20,502,349 (GRCm38)	I17M	possibly damaging	Het
Vmn1r232	A	G	17: 20,914,203 (GRCm38)	L45P	probably benign	Het
Wdr7	T	A	18: 63,777,607 (GRCm38)	V690E	possibly damaging	Het
Xaf1	A	T	11: 72,303,402 (GRCm38)	E36D	possibly damaging	Het
Zfp131	A	T	13: 119,775,812 (GRCm38)	F303I	probably damaging	Het
Zfp513	T	G	5: 31,200,423 (GRCm38)	K202T	probably damaging	Het
Zfp788	A	T	7: 41,649,640 (GRCm38)	I567F	probably damaging	Het
Zfyve26	A	G	12: 79,246,087 (GRCm38)	V2096A	probably damaging	Het

Other mutations in Tg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Tg	APN	15	66,847,166 (GRCm38)	missense	probably damaging	1.00
IGL00230:Tg	APN	15	66,827,290 (GRCm38)	missense	probably benign	0.00
IGL00324:Tg	APN	15	66,693,424 (GRCm38)	missense	probably benign
IGL00428:Tg	APN	15	66,773,424 (GRCm38)	missense	probably benign	0.33
IGL00703:Tg	APN	15	66,696,489 (GRCm38)	missense	probably benign	0.34
IGL00808:Tg	APN	15	66,683,813 (GRCm38)	missense	probably damaging	1.00
IGL00833:Tg	APN	15	66,688,801 (GRCm38)	missense	probably benign	0.34
IGL00899:Tg	APN	15	66,674,073 (GRCm38)	critical splice donor site	probably null
IGL00921:Tg	APN	15	66,764,453 (GRCm38)	missense	probably benign	0.28
IGL00975:Tg	APN	15	66,681,882 (GRCm38)	missense	probably benign
IGL01288:Tg	APN	15	66,736,276 (GRCm38)	missense	possibly damaging	0.81
IGL01397:Tg	APN	15	66,696,092 (GRCm38)	splice site	probably benign
IGL01634:Tg	APN	15	66,729,566 (GRCm38)	missense	probably benign	0.34
IGL01646:Tg	APN	15	66,678,087 (GRCm38)	missense	probably damaging	1.00
IGL01704:Tg	APN	15	66,671,351 (GRCm38)	missense	probably damaging	0.98
IGL01958:Tg	APN	15	66,759,486 (GRCm38)	missense	probably benign	0.06
IGL02093:Tg	APN	15	66,692,374 (GRCm38)	missense	possibly damaging	0.83
IGL02113:Tg	APN	15	66,705,330 (GRCm38)	missense	probably benign	0.08
IGL02138:Tg	APN	15	66,717,233 (GRCm38)	missense	probably benign	0.01
IGL02156:Tg	APN	15	66,705,348 (GRCm38)	missense	probably benign	0.19
IGL02169:Tg	APN	15	66,757,943 (GRCm38)	missense	probably benign	0.04
IGL02342:Tg	APN	15	66,764,291 (GRCm38)	missense	probably benign
IGL02434:Tg	APN	15	66,764,342 (GRCm38)	missense	probably damaging	0.97
IGL02506:Tg	APN	15	66,741,594 (GRCm38)	missense	possibly damaging	0.71
IGL02513:Tg	APN	15	66,705,274 (GRCm38)	missense	probably benign
IGL02549:Tg	APN	15	66,839,361 (GRCm38)	missense	probably damaging	1.00
IGL02669:Tg	APN	15	66,748,726 (GRCm38)	splice site	probably benign
IGL02756:Tg	APN	15	66,734,586 (GRCm38)	missense	probably benign
IGL02800:Tg	APN	15	66,757,886 (GRCm38)	missense	probably damaging	1.00
IGL02828:Tg	APN	15	66,682,394 (GRCm38)	missense	probably damaging	1.00
IGL02927:Tg	APN	15	66,678,093 (GRCm38)	missense	probably damaging	1.00
IGL03061:Tg	APN	15	66,671,405 (GRCm38)	missense	probably damaging	1.00
IGL03105:Tg	APN	15	66,715,106 (GRCm38)	missense	probably benign	0.01
IGL03160:Tg	APN	15	66,839,303 (GRCm38)	nonsense	probably null
IGL03242:Tg	APN	15	66,683,798 (GRCm38)	missense	probably damaging	0.99
Also_ran	UTSW	15	66,678,839 (GRCm38)	missense	probably damaging	1.00
bedraggled	UTSW	15	66,740,714 (GRCm38)	missense	probably damaging	1.00
foster	UTSW	15	66,693,260 (GRCm38)	nonsense	probably null
hognose	UTSW	15	66,717,208 (GRCm38)	missense	probably damaging	0.99
ito	UTSW	15	66,766,162 (GRCm38)	nonsense	probably null
ito2	UTSW	15	66,671,396 (GRCm38)	missense	probably damaging	1.00
ito3	UTSW	15	66,773,474 (GRCm38)	missense	probably damaging	1.00
ito4	UTSW	15	66,696,520 (GRCm38)	missense	possibly damaging	0.47
Papua	UTSW	15	66,674,050 (GRCm38)	missense	probably damaging	1.00
Pipistrella	UTSW	15	66,696,135 (GRCm38)	missense	probably damaging	1.00
pluribus	UTSW	15	66,715,163 (GRCm38)	missense	probably damaging	0.98
samarai	UTSW	15	66,758,006 (GRCm38)	critical splice donor site	probably null
sariba	UTSW	15	66,694,870 (GRCm38)	missense	probably benign	0.01
ticker	UTSW	15	66,827,382 (GRCm38)	nonsense	probably null
Vampire	UTSW	15	66,682,827 (GRCm38)	missense	probably damaging	1.00
IGL03134:Tg	UTSW	15	66,740,718 (GRCm38)	missense	probably damaging	1.00
P0019:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
R0121:Tg	UTSW	15	66,740,781 (GRCm38)	missense	probably benign	0.04
R0135:Tg	UTSW	15	66,694,870 (GRCm38)	missense	probably benign	0.01
R0227:Tg	UTSW	15	66,698,446 (GRCm38)	missense	possibly damaging	0.84
R0448:Tg	UTSW	15	66,764,442 (GRCm38)	missense	probably damaging	1.00
R0453:Tg	UTSW	15	66,828,533 (GRCm38)	missense	probably benign	0.09
R0504:Tg	UTSW	15	66,682,404 (GRCm38)	missense	probably damaging	0.97
R0543:Tg	UTSW	15	66,729,597 (GRCm38)	missense	probably benign	0.13
R0638:Tg	UTSW	15	66,717,208 (GRCm38)	missense	probably damaging	0.99
R0639:Tg	UTSW	15	66,741,484 (GRCm38)	critical splice acceptor site	probably null
R0646:Tg	UTSW	15	66,729,626 (GRCm38)	missense	probably damaging	0.99
R0666:Tg	UTSW	15	66,737,521 (GRCm38)	missense	probably benign
R0673:Tg	UTSW	15	66,741,484 (GRCm38)	critical splice acceptor site	probably null
R0689:Tg	UTSW	15	66,839,404 (GRCm38)	splice site	probably benign
R0704:Tg	UTSW	15	66,757,880 (GRCm38)	missense	probably benign	0.02
R0730:Tg	UTSW	15	66,678,789 (GRCm38)	missense	probably damaging	1.00
R0830:Tg	UTSW	15	66,725,144 (GRCm38)	missense	probably damaging	1.00
R0959:Tg	UTSW	15	66,708,010 (GRCm38)	missense	probably damaging	0.98
R1027:Tg	UTSW	15	66,672,409 (GRCm38)	missense	possibly damaging	0.65
R1061:Tg	UTSW	15	66,698,559 (GRCm38)	missense	probably benign	0.09
R1086:Tg	UTSW	15	66,684,062 (GRCm38)	missense	probably benign
R1103:Tg	UTSW	15	66,719,655 (GRCm38)	missense	probably benign	0.45
R1240:Tg	UTSW	15	66,828,548 (GRCm38)	missense	probably benign	0.16
R1281:Tg	UTSW	15	66,696,489 (GRCm38)	missense	probably benign	0.34
R1470:Tg	UTSW	15	66,849,463 (GRCm38)	missense	possibly damaging	0.95
R1470:Tg	UTSW	15	66,849,463 (GRCm38)	missense	possibly damaging	0.95
R1531:Tg	UTSW	15	66,850,502 (GRCm38)	missense	probably benign	0.02
R1544:Tg	UTSW	15	66,705,232 (GRCm38)	missense	probably benign	0.04
R1550:Tg	UTSW	15	66,693,430 (GRCm38)	missense	possibly damaging	0.52
R1575:Tg	UTSW	15	66,729,685 (GRCm38)	critical splice donor site	probably null
R1638:Tg	UTSW	15	66,696,166 (GRCm38)	nonsense	probably null
R1655:Tg	UTSW	15	66,828,568 (GRCm38)	critical splice donor site	probably null
R1671:Tg	UTSW	15	66,692,387 (GRCm38)	missense	possibly damaging	0.89
R1789:Tg	UTSW	15	66,737,548 (GRCm38)	missense	probably benign	0.00
R1883:Tg	UTSW	15	66,671,309 (GRCm38)	missense	probably damaging	1.00
R1984:Tg	UTSW	15	66,682,842 (GRCm38)	missense	probably benign
R2063:Tg	UTSW	15	66,828,553 (GRCm38)	missense	probably damaging	1.00
R2092:Tg	UTSW	15	66,849,607 (GRCm38)	missense	probably null	0.26
R2109:Tg	UTSW	15	66,729,594 (GRCm38)	missense	probably benign	0.02
R2128:Tg	UTSW	15	66,694,894 (GRCm38)	missense	probably benign	0.10
R2129:Tg	UTSW	15	66,694,894 (GRCm38)	missense	probably benign	0.10
R2219:Tg	UTSW	15	66,681,933 (GRCm38)	missense	probably benign	0.03
R2228:Tg	UTSW	15	66,674,011 (GRCm38)	missense	probably damaging	0.99
R2229:Tg	UTSW	15	66,674,011 (GRCm38)	missense	probably damaging	0.99
R2259:Tg	UTSW	15	66,683,898 (GRCm38)	missense	probably benign
R2994:Tg	UTSW	15	66,681,953 (GRCm38)	missense	probably benign
R3904:Tg	UTSW	15	66,766,162 (GRCm38)	nonsense	probably null
R3946:Tg	UTSW	15	66,674,023 (GRCm38)	missense	probably damaging	1.00
R3965:Tg	UTSW	15	66,684,190 (GRCm38)	missense	probably benign
R4245:Tg	UTSW	15	66,696,469 (GRCm38)	missense	possibly damaging	0.68
R4451:Tg	UTSW	15	66,766,147 (GRCm38)	missense	probably benign	0.01
R4487:Tg	UTSW	15	66,671,396 (GRCm38)	missense	probably damaging	1.00
R4489:Tg	UTSW	15	66,707,942 (GRCm38)	missense	probably damaging	1.00
R4623:Tg	UTSW	15	66,735,271 (GRCm38)	missense	probably benign	0.23
R4659:Tg	UTSW	15	66,673,920 (GRCm38)	missense	possibly damaging	0.67
R4728:Tg	UTSW	15	66,682,827 (GRCm38)	missense	probably damaging	1.00
R4760:Tg	UTSW	15	66,693,319 (GRCm38)	missense	probably damaging	1.00
R4797:Tg	UTSW	15	66,758,006 (GRCm38)	critical splice donor site	probably null
R4944:Tg	UTSW	15	66,764,337 (GRCm38)	missense	probably damaging	1.00
R4998:Tg	UTSW	15	66,674,050 (GRCm38)	missense	probably damaging	1.00
R5009:Tg	UTSW	15	66,696,586 (GRCm38)	missense	probably benign	0.01
R5025:Tg	UTSW	15	66,707,930 (GRCm38)	missense	probably damaging	1.00
R5035:Tg	UTSW	15	66,681,813 (GRCm38)	splice site	probably null
R5049:Tg	UTSW	15	66,827,382 (GRCm38)	nonsense	probably null
R5073:Tg	UTSW	15	66,735,252 (GRCm38)	missense	probably benign	0.05
R5169:Tg	UTSW	15	66,678,780 (GRCm38)	nonsense	probably null
R5185:Tg	UTSW	15	66,773,474 (GRCm38)	missense	probably damaging	1.00
R5227:Tg	UTSW	15	66,759,567 (GRCm38)	missense	possibly damaging	0.87
R5300:Tg	UTSW	15	66,678,855 (GRCm38)	missense	probably damaging	1.00
R5334:Tg	UTSW	15	66,678,055 (GRCm38)	missense	probably damaging	1.00
R5339:Tg	UTSW	15	66,678,093 (GRCm38)	missense	probably damaging	1.00
R5402:Tg	UTSW	15	66,739,168 (GRCm38)	missense	probably damaging	0.98
R5441:Tg	UTSW	15	66,696,520 (GRCm38)	missense	possibly damaging	0.47
R5509:Tg	UTSW	15	66,827,293 (GRCm38)	missense	probably benign	0.45
R5580:Tg	UTSW	15	66,685,300 (GRCm38)	missense	possibly damaging	0.66
R5582:Tg	UTSW	15	66,693,435 (GRCm38)	missense	probably damaging	1.00
R5624:Tg	UTSW	15	66,838,057 (GRCm38)	missense	probably benign	0.11
R5686:Tg	UTSW	15	66,688,889 (GRCm38)	missense	probably benign	0.28
R6042:Tg	UTSW	15	66,683,993 (GRCm38)	missense	probably benign	0.01
R6122:Tg	UTSW	15	66,828,457 (GRCm38)	missense	probably damaging	1.00
R6146:Tg	UTSW	15	66,673,367 (GRCm38)	splice site	probably null
R6159:Tg	UTSW	15	66,735,247 (GRCm38)	missense	possibly damaging	0.71
R6223:Tg	UTSW	15	66,707,922 (GRCm38)	missense	probably benign	0.15
R6480:Tg	UTSW	15	66,671,311 (GRCm38)	missense	probably damaging	1.00
R6505:Tg	UTSW	15	66,759,558 (GRCm38)	missense	probably damaging	0.99
R6531:Tg	UTSW	15	66,839,362 (GRCm38)	missense	probably damaging	0.99
R6614:Tg	UTSW	15	66,735,259 (GRCm38)	missense	probably damaging	0.99
R6698:Tg	UTSW	15	66,839,362 (GRCm38)	missense	probably damaging	1.00
R6798:Tg	UTSW	15	66,678,839 (GRCm38)	missense	probably damaging	1.00
R6837:Tg	UTSW	15	66,696,135 (GRCm38)	missense	probably damaging	1.00
R6861:Tg	UTSW	15	66,688,891 (GRCm38)	missense	probably benign	0.00
R6888:Tg	UTSW	15	66,696,246 (GRCm38)	missense	probably damaging	0.99
R6933:Tg	UTSW	15	66,764,309 (GRCm38)	missense	possibly damaging	0.73
R6983:Tg	UTSW	15	66,693,358 (GRCm38)	missense	probably benign	0.01
R7078:Tg	UTSW	15	66,673,543 (GRCm38)	missense	probably damaging	1.00
R7244:Tg	UTSW	15	66,740,714 (GRCm38)	missense	probably damaging	1.00
R7320:Tg	UTSW	15	66,694,784 (GRCm38)	missense	possibly damaging	0.71
R7334:Tg	UTSW	15	66,725,272 (GRCm38)	missense	probably benign	0.01
R7418:Tg	UTSW	15	66,696,583 (GRCm38)	missense	probably damaging	0.99
R7485:Tg	UTSW	15	66,696,588 (GRCm38)	missense	probably benign	0.04
R7524:Tg	UTSW	15	66,696,161 (GRCm38)	missense	probably benign	0.01
R7529:Tg	UTSW	15	66,694,768 (GRCm38)	missense	probably damaging	0.99
R7540:Tg	UTSW	15	66,689,927 (GRCm38)	missense	probably benign	0.16
R7583:Tg	UTSW	15	66,764,418 (GRCm38)	missense	probably damaging	1.00
R7594:Tg	UTSW	15	66,729,583 (GRCm38)	missense	probably benign	0.20
R7667:Tg	UTSW	15	66,715,163 (GRCm38)	missense	probably damaging	0.98
R7722:Tg	UTSW	15	66,764,309 (GRCm38)	missense	possibly damaging	0.73
R7790:Tg	UTSW	15	66,849,604 (GRCm38)	missense	probably damaging	0.99
R7838:Tg	UTSW	15	66,693,263 (GRCm38)	missense	probably benign	0.00
R7890:Tg	UTSW	15	66,683,814 (GRCm38)	missense	probably damaging	1.00
R7904:Tg	UTSW	15	66,705,279 (GRCm38)	missense	probably benign	0.08
R7919:Tg	UTSW	15	66,684,074 (GRCm38)	missense	possibly damaging	0.73
R7921:Tg	UTSW	15	66,683,793 (GRCm38)	missense	probably benign	0.08
R8037:Tg	UTSW	15	66,688,875 (GRCm38)	missense	probably benign	0.00
R8038:Tg	UTSW	15	66,688,875 (GRCm38)	missense	probably benign	0.00
R8214:Tg	UTSW	15	66,773,398 (GRCm38)	missense	probably damaging	1.00
R8304:Tg	UTSW	15	66,693,260 (GRCm38)	nonsense	probably null
R8688:Tg	UTSW	15	66,694,953 (GRCm38)	critical splice donor site	probably benign
R8709:Tg	UTSW	15	66,681,937 (GRCm38)	missense	probably benign	0.08
R8714:Tg	UTSW	15	66,684,042 (GRCm38)	missense	probably damaging	0.97
R8901:Tg	UTSW	15	66,685,335 (GRCm38)	missense	probably damaging	1.00
R8917:Tg	UTSW	15	66,773,483 (GRCm38)	critical splice donor site	probably null
R9023:Tg	UTSW	15	66,683,673 (GRCm38)	missense	probably damaging	1.00
R9232:Tg	UTSW	15	66,698,461 (GRCm38)	missense	probably benign	0.01
R9310:Tg	UTSW	15	66,827,269 (GRCm38)	missense	possibly damaging	0.69
R9361:Tg	UTSW	15	66,685,397 (GRCm38)	missense	possibly damaging	0.50
R9389:Tg	UTSW	15	66,689,324 (GRCm38)	missense	probably benign	0.04
R9501:Tg	UTSW	15	66,847,074 (GRCm38)	missense	possibly damaging	0.52
R9510:Tg	UTSW	15	66,674,064 (GRCm38)	missense	probably damaging	1.00
R9594:Tg	UTSW	15	66,735,260 (GRCm38)	nonsense	probably null
R9629:Tg	UTSW	15	66,683,738 (GRCm38)	missense	possibly damaging	0.95
R9701:Tg	UTSW	15	66,766,142 (GRCm38)	missense	probably benign	0.03
R9743:Tg	UTSW	15	66,689,990 (GRCm38)	missense	probably benign	0.18
R9748:Tg	UTSW	15	66,847,159 (GRCm38)	missense	possibly damaging	0.91
T0975:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
X0005:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
X0065:Tg	UTSW	15	66,682,454 (GRCm38)	missense	probably damaging	1.00
X0067:Tg	UTSW	15	66,748,743 (GRCm38)	missense	probably benign	0.10
Z1177:Tg	UTSW	15	66,849,547 (GRCm38)	missense	probably benign	0.02
Z1177:Tg	UTSW	15	66,685,310 (GRCm38)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- GAAGGGTCCGGAGAACTACTTG -3'
(R):5'- ATGTCCCTCCAAACAGGTTCTG -3'

Sequencing Primer
(F):5'- GAACTACTTGTTCCGCTAACTATGG -3'
(R):5'- TCCAAACAGGTTCTGCTGGAGTC -3'

Posted On

2014-10-02