Mutagenetix > Incidental Mutation

Incidental Mutation 'R2398:Vmn2r67'

248596

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r67

Ensembl Gene

ENSMUSG00000095664

Gene Name

vomeronasal 2, receptor 67

Synonyms

EG620672

MMRRC Submission

040365-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.111) question?

Stock #

R2398 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

84785448-84805110 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 84785921 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 695 (I695V)

Ref Sequence

ENSEMBL: ENSMUSP00000126007 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168730]

AlphaFold

K7N6T2

Predicted Effect

probably damaging
Transcript: ENSMUST00000168730
AA Change: I695V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126007
Gene: ENSMUSG00000095664
AA Change: I695V

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:ANF_receptor	77	464	2.1e-31	PFAM
Pfam:NCD3G	507	559	4.8e-19	PFAM
Pfam:7tm_3	590	827	1.4e-53	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.8%
20x: 93.4%

Validation Efficiency

100% (39/39)

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930568D16Rik	T	C	2: 35,244,872 (GRCm39)	D160G	possibly damaging	Het
Akr1c6	T	C	13: 4,499,035 (GRCm39)	S208P	probably benign	Het
Ap3b2	A	G	7: 81,126,943 (GRCm39)	F269S	probably damaging	Het
Ap3d1	G	A	10: 80,555,006 (GRCm39)	Q440*	probably null	Het
Cog2	T	C	8: 125,256,665 (GRCm39)	I137T	probably benign	Het
Cse1l	A	G	2: 166,770,917 (GRCm39)	Y369C	probably damaging	Het
Dnah9	T	C	11: 65,806,029 (GRCm39)	N3357D	probably damaging	Het
Fam83b	T	C	9: 76,409,500 (GRCm39)	T209A	probably damaging	Het
Gbp2	G	A	3: 142,339,123 (GRCm39)	A392T	probably benign	Het
Grsf1	G	A	5: 88,821,695 (GRCm39)	T123I	probably damaging	Het
Gzmc	T	A	14: 56,470,228 (GRCm39)	I90F	possibly damaging	Het
Hook2	T	A	8: 85,717,928 (GRCm39)	N42K	probably damaging	Het
Hsp90aa1	T	C	12: 110,658,755 (GRCm39)	M629V	possibly damaging	Het
Ifna14	T	C	4: 88,489,863 (GRCm39)	D58G	possibly damaging	Het
Krtap4-8	T	C	11: 99,671,103 (GRCm39)		probably benign	Het
Mocs1	T	C	17: 49,759,862 (GRCm39)	I381T	probably damaging	Het
Nbas	A	G	12: 13,482,946 (GRCm39)	T1408A	probably damaging	Het
Or6c219	A	G	10: 129,781,076 (GRCm39)	F285S	probably benign	Het
Or8j3b	T	A	2: 86,205,183 (GRCm39)	D191V	probably damaging	Het
Orc1	C	T	4: 108,459,166 (GRCm39)	T445I	possibly damaging	Het
Pdik1l	T	C	4: 134,005,710 (GRCm39)	K411E	probably benign	Het
Pias3	A	G	3: 96,611,129 (GRCm39)	I443V	probably benign	Het
Psmd2	T	C	16: 20,478,222 (GRCm39)	S536P	possibly damaging	Het
Rnf10	C	T	5: 115,385,332 (GRCm39)	R554H	probably benign	Het
Smarcc2	C	T	10: 128,305,551 (GRCm39)	T325I	possibly damaging	Het
Smchd1	C	A	17: 71,667,136 (GRCm39)	C1752F	probably damaging	Het
Smchd1	G	A	17: 71,733,431 (GRCm39)		probably benign	Het
Svil	A	G	18: 5,060,613 (GRCm39)		probably null	Het
Tex52	T	C	6: 128,356,540 (GRCm39)	S78P	probably damaging	Het
Tmem63c	T	G	12: 87,103,307 (GRCm39)	V27G	probably damaging	Het
Ttc41	A	G	10: 86,549,250 (GRCm39)	N148S	possibly damaging	Het
Wipf2	T	A	11: 98,789,543 (GRCm39)		probably null	Het
Zc3h18	A	G	8: 123,140,605 (GRCm39)		probably benign	Het
Zfp804a	T	A	2: 82,089,013 (GRCm39)	N947K	possibly damaging	Het
Zfyve26	T	C	12: 79,329,573 (GRCm39)		probably null	Het
Zic2	G	T	14: 122,716,329 (GRCm39)	E422*	probably null	Het
Zmym4	A	T	4: 126,816,929 (GRCm39)	D4E	probably damaging	Het

Other mutations in Vmn2r67

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00096:Vmn2r67	APN	7	84,801,138 (GRCm39)	missense	probably damaging	1.00
IGL01346:Vmn2r67	APN	7	84,786,127 (GRCm39)	missense	probably damaging	1.00
IGL01373:Vmn2r67	APN	7	84,785,834 (GRCm39)	missense	probably benign	0.10
IGL01674:Vmn2r67	APN	7	84,785,651 (GRCm39)	missense	probably damaging	1.00
IGL01978:Vmn2r67	APN	7	84,800,649 (GRCm39)	critical splice donor site	probably null
IGL02013:Vmn2r67	APN	7	84,800,863 (GRCm39)	missense	probably benign	0.09
IGL02115:Vmn2r67	APN	7	84,800,787 (GRCm39)	missense	probably damaging	0.99
IGL02250:Vmn2r67	APN	7	84,805,008 (GRCm39)	missense	probably benign
IGL02252:Vmn2r67	APN	7	84,805,008 (GRCm39)	missense	probably benign
IGL02328:Vmn2r67	APN	7	84,799,898 (GRCm39)	missense	probably benign	0.41
IGL02740:Vmn2r67	APN	7	84,785,818 (GRCm39)	missense	probably damaging	1.00
IGL02940:Vmn2r67	APN	7	84,785,951 (GRCm39)	missense	probably benign	0.07
IGL03237:Vmn2r67	APN	7	84,799,118 (GRCm39)	missense	probably damaging	1.00
R0512:Vmn2r67	UTSW	7	84,799,900 (GRCm39)	missense	probably damaging	1.00
R1029:Vmn2r67	UTSW	7	84,785,974 (GRCm39)	missense	probably damaging	1.00
R1193:Vmn2r67	UTSW	7	84,800,653 (GRCm39)	missense	probably damaging	0.98
R1282:Vmn2r67	UTSW	7	84,785,932 (GRCm39)	missense	probably benign
R1416:Vmn2r67	UTSW	7	84,800,824 (GRCm39)	missense	probably benign	0.06
R1429:Vmn2r67	UTSW	7	84,802,031 (GRCm39)	missense	possibly damaging	0.65
R1462:Vmn2r67	UTSW	7	84,805,046 (GRCm39)	missense	probably benign	0.00
R1462:Vmn2r67	UTSW	7	84,805,046 (GRCm39)	missense	probably benign	0.00
R1970:Vmn2r67	UTSW	7	84,801,013 (GRCm39)	missense	probably benign
R2229:Vmn2r67	UTSW	7	84,801,250 (GRCm39)	missense	probably benign	0.21
R2246:Vmn2r67	UTSW	7	84,785,764 (GRCm39)	missense	probably damaging	1.00
R2262:Vmn2r67	UTSW	7	84,786,182 (GRCm39)	missense	probably damaging	0.96
R4249:Vmn2r67	UTSW	7	84,799,722 (GRCm39)	splice site	probably null
R4666:Vmn2r67	UTSW	7	84,799,831 (GRCm39)	missense	probably benign
R4669:Vmn2r67	UTSW	7	84,799,732 (GRCm39)	missense	probably benign	0.11
R4966:Vmn2r67	UTSW	7	84,785,593 (GRCm39)	missense	probably damaging	1.00
R5264:Vmn2r67	UTSW	7	84,801,453 (GRCm39)	missense	probably damaging	1.00
R5296:Vmn2r67	UTSW	7	84,786,230 (GRCm39)	missense	probably damaging	1.00
R5327:Vmn2r67	UTSW	7	84,785,698 (GRCm39)	missense	probably damaging	1.00
R5401:Vmn2r67	UTSW	7	84,785,765 (GRCm39)	missense	probably damaging	1.00
R5510:Vmn2r67	UTSW	7	84,801,023 (GRCm39)	missense	probably benign	0.39
R5574:Vmn2r67	UTSW	7	84,801,099 (GRCm39)	missense	probably benign	0.00
R5643:Vmn2r67	UTSW	7	84,799,151 (GRCm39)	nonsense	probably null
R5914:Vmn2r67	UTSW	7	84,801,044 (GRCm39)	missense	probably damaging	1.00
R6248:Vmn2r67	UTSW	7	84,799,768 (GRCm39)	missense	probably damaging	0.99
R6291:Vmn2r67	UTSW	7	84,799,142 (GRCm39)	missense	possibly damaging	0.88
R6309:Vmn2r67	UTSW	7	84,801,124 (GRCm39)	missense	probably benign
R6442:Vmn2r67	UTSW	7	84,805,046 (GRCm39)	missense	possibly damaging	0.82
R6665:Vmn2r67	UTSW	7	84,785,900 (GRCm39)	missense	probably benign	0.07
R6701:Vmn2r67	UTSW	7	84,802,023 (GRCm39)	missense	probably damaging	1.00
R6848:Vmn2r67	UTSW	7	84,801,840 (GRCm39)	missense	probably benign	0.00
R6852:Vmn2r67	UTSW	7	84,801,361 (GRCm39)	missense	probably damaging	0.99
R6991:Vmn2r67	UTSW	7	84,804,953 (GRCm39)	missense	possibly damaging	0.55
R7143:Vmn2r67	UTSW	7	84,801,846 (GRCm39)	missense	probably benign
R7197:Vmn2r67	UTSW	7	84,785,774 (GRCm39)	missense	possibly damaging	0.77
R7393:Vmn2r67	UTSW	7	84,805,086 (GRCm39)	missense	probably null	0.87
R7420:Vmn2r67	UTSW	7	84,785,944 (GRCm39)	missense	possibly damaging	0.52
R7622:Vmn2r67	UTSW	7	84,785,662 (GRCm39)	missense	probably damaging	1.00
R7664:Vmn2r67	UTSW	7	84,805,019 (GRCm39)	missense	probably benign	0.21
R7665:Vmn2r67	UTSW	7	84,801,196 (GRCm39)	nonsense	probably null
R7896:Vmn2r67	UTSW	7	84,785,920 (GRCm39)	missense	probably damaging	1.00
R7913:Vmn2r67	UTSW	7	84,801,036 (GRCm39)	missense	possibly damaging	0.87
R8026:Vmn2r67	UTSW	7	84,785,924 (GRCm39)	missense	probably damaging	1.00
R8114:Vmn2r67	UTSW	7	84,805,097 (GRCm39)	missense	probably benign	0.01
R8317:Vmn2r67	UTSW	7	84,785,834 (GRCm39)	missense	probably benign	0.10
R8363:Vmn2r67	UTSW	7	84,804,969 (GRCm39)	missense	probably benign	0.00
R8421:Vmn2r67	UTSW	7	84,785,893 (GRCm39)	missense	probably damaging	0.98
R8444:Vmn2r67	UTSW	7	84,785,854 (GRCm39)	missense	probably benign	0.01
R8751:Vmn2r67	UTSW	7	84,801,450 (GRCm39)	missense	probably benign	0.01
R8810:Vmn2r67	UTSW	7	84,786,346 (GRCm39)	missense	probably damaging	1.00
R8811:Vmn2r67	UTSW	7	84,799,895 (GRCm39)	missense	probably damaging	0.98
R9215:Vmn2r67	UTSW	7	84,802,008 (GRCm39)	missense	probably benign	0.00
R9342:Vmn2r67	UTSW	7	84,785,788 (GRCm39)	missense	probably benign	0.00
R9433:Vmn2r67	UTSW	7	84,804,917 (GRCm39)	missense	possibly damaging	0.60
R9453:Vmn2r67	UTSW	7	84,800,697 (GRCm39)	missense	probably benign	0.32
R9471:Vmn2r67	UTSW	7	84,799,723 (GRCm39)	critical splice donor site	probably null
R9526:Vmn2r67	UTSW	7	84,785,834 (GRCm39)	missense	probably benign	0.10
R9538:Vmn2r67	UTSW	7	84,801,327 (GRCm39)	missense
R9544:Vmn2r67	UTSW	7	84,786,317 (GRCm39)	missense	possibly damaging	0.53
R9574:Vmn2r67	UTSW	7	84,786,017 (GRCm39)	missense	probably benign	0.00
R9599:Vmn2r67	UTSW	7	84,804,941 (GRCm39)	missense	probably damaging	0.96
R9768:Vmn2r67	UTSW	7	84,802,037 (GRCm39)	missense	probably benign	0.23

Predicted Primers

PCR Primer
(F):5'- GGCTTCATTGAATGTATCAGGC -3'
(R):5'- CATCCCACTACGGTCATCTG -3'

Sequencing Primer
(F):5'- GCTACAGTGAAACTTGCTAGAGC -3'
(R):5'- ACGGTCATCTGTATCCTGCAG -3'

Posted On

2014-11-11