Mutagenetix > Incidental Mutation

Incidental Mutation 'R2971:Tmem201'

265672

Institutional Source

Beutler Lab

Gene Symbol

Tmem201

Ensembl Gene

ENSMUSG00000044700

Gene Name

transmembrane protein 201

Synonyms

Samp1, D4Ertd429e

MMRRC Submission

040525-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.426) question?

Stock #

R2971 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

149799832-149822501 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to G at 149806902 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000101312 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054459] [ENSMUST00000103208] [ENSMUST00000105687]

AlphaFold

A2A8U2

Predicted Effect

probably benign
Transcript: ENSMUST00000054459

SMART Domains

Protein: ENSMUSP00000050481
Gene: ENSMUSG00000044700

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
Pfam:Ima1_N	46	171	2.6e-43	PFAM
Pfam:DUF2448	191	392	4.4e-102	PFAM
low complexity region	433	455	N/A	INTRINSIC
low complexity region	486	521	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000103208

SMART Domains

Protein: ENSMUSP00000099497
Gene: ENSMUSG00000044700

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
Pfam:Ima1_N	46	171	1.1e-43	PFAM
Pfam:DUF2448	191	392	2.1e-103	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105687

SMART Domains

Protein: ENSMUSP00000101312
Gene: ENSMUSG00000044700

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
Pfam:Ima1_N	46	171	2.4e-39	PFAM
Pfam:DUF2448	191	389	3.1e-96	PFAM
low complexity region	433	455	N/A	INTRINSIC
low complexity region	486	521	N/A	INTRINSIC
transmembrane domain	638	660	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139923

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.6%

Validation Efficiency

100% (35/35)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acan	A	G	7: 78,749,447 (GRCm39)	D1406G	possibly damaging	Het
Aebp2	T	C	6: 140,579,624 (GRCm39)		probably null	Het
Ap5m1	T	A	14: 49,321,339 (GRCm39)	Y49*	probably null	Het
Atp8b5	T	C	4: 43,361,953 (GRCm39)		probably benign	Het
Baz1a	A	G	12: 54,970,224 (GRCm39)	S518P	probably damaging	Het
Ces1c	T	A	8: 93,830,821 (GRCm39)	D445V	probably benign	Het
Ctnnbl1	C	T	2: 157,713,106 (GRCm39)	H464Y	probably benign	Het
Cyp2j6	T	C	4: 96,420,018 (GRCm39)	K238E	probably benign	Het
Gdf10	G	A	14: 33,646,148 (GRCm39)	R99H	probably damaging	Het
Gm4779	G	A	X: 100,836,568 (GRCm39)	P116L	possibly damaging	Het
Gucy2g	A	G	19: 55,198,708 (GRCm39)	S812P	probably damaging	Het
Ifit3b	C	T	19: 34,589,417 (GRCm39)	Q198*	probably null	Het
Irgm1	A	T	11: 48,757,417 (GRCm39)	Y131*	probably null	Het
Man1c1	G	C	4: 134,430,749 (GRCm39)	P11R	probably damaging	Het
Myh7b	A	G	2: 155,474,175 (GRCm39)	N1630S	probably benign	Het
Myo5a	T	C	9: 75,023,484 (GRCm39)	I15T	probably damaging	Het
Naip6	C	T	13: 100,437,108 (GRCm39)	A472T	probably benign	Het
Nme6	A	G	9: 109,671,159 (GRCm39)		probably benign	Het
Or11g25	T	A	14: 50,723,065 (GRCm39)	I50N	probably damaging	Het
Or5al6	T	C	2: 85,976,908 (GRCm39)	T57A	probably damaging	Het
Plch2	T	G	4: 155,075,224 (GRCm39)	M797L	probably benign	Het
Plscr2	G	T	9: 92,172,724 (GRCm39)	E128*	probably null	Het
Plxna2	T	A	1: 194,480,039 (GRCm39)	D1403E	probably damaging	Het
Pou6f2	T	C	13: 18,556,552 (GRCm39)	T25A	unknown	Het
Psmb11	T	C	14: 54,862,800 (GRCm39)	V6A	possibly damaging	Het
Ptch1	T	G	13: 63,672,773 (GRCm39)	E944A	probably benign	Het
Ptprd	T	G	4: 76,025,561 (GRCm39)	S546R	probably benign	Het
Rbp3	A	G	14: 33,676,411 (GRCm39)	N120D	probably benign	Het
Skint1	C	A	4: 111,878,527 (GRCm39)	P153H	possibly damaging	Het
Slc15a4	A	G	5: 127,681,600 (GRCm39)		probably null	Het
Ube2v1	T	C	2: 167,452,256 (GRCm39)	N89D	probably damaging	Het
Zfp282	A	T	6: 47,874,866 (GRCm39)		probably null	Het
Zfp560	C	A	9: 20,260,240 (GRCm39)	M207I	probably benign	Het
Zfp697	T	C	3: 98,335,617 (GRCm39)	Y461H	probably damaging	Het

Other mutations in Tmem201

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01323:Tmem201	APN	4	149,804,045 (GRCm39)	splice site	probably benign
R0508:Tmem201	UTSW	4	149,816,343 (GRCm39)	missense	probably damaging	1.00
R0717:Tmem201	UTSW	4	149,803,267 (GRCm39)	missense	probably damaging	1.00
R1660:Tmem201	UTSW	4	149,804,032 (GRCm39)	missense	probably damaging	1.00
R2138:Tmem201	UTSW	4	149,802,537 (GRCm39)	missense	probably damaging	1.00
R4430:Tmem201	UTSW	4	149,815,596 (GRCm39)	missense	probably benign	0.03
R4704:Tmem201	UTSW	4	149,811,774 (GRCm39)	missense	possibly damaging	0.77
R4876:Tmem201	UTSW	4	149,806,727 (GRCm39)	missense	probably damaging	1.00
R4966:Tmem201	UTSW	4	149,803,144 (GRCm39)	missense	probably benign
R4991:Tmem201	UTSW	4	149,812,612 (GRCm39)	missense	possibly damaging	0.95
R5518:Tmem201	UTSW	4	149,802,534 (GRCm39)	missense	probably benign
R5818:Tmem201	UTSW	4	149,811,849 (GRCm39)	missense	probably benign	0.33
R7399:Tmem201	UTSW	4	149,815,554 (GRCm39)	missense	possibly damaging	0.95
R8142:Tmem201	UTSW	4	149,803,114 (GRCm39)	missense	probably benign
R8170:Tmem201	UTSW	4	149,803,177 (GRCm39)	missense	probably benign	0.29
R8294:Tmem201	UTSW	4	149,815,554 (GRCm39)	missense	possibly damaging	0.95
R8513:Tmem201	UTSW	4	149,812,380 (GRCm39)	missense	probably damaging	0.99
R8808:Tmem201	UTSW	4	149,814,138 (GRCm39)	missense	possibly damaging	0.79
R9026:Tmem201	UTSW	4	149,812,627 (GRCm39)	missense	probably benign	0.08
X0064:Tmem201	UTSW	4	149,802,528 (GRCm39)	missense	possibly damaging	0.75

Predicted Primers

PCR Primer
(F):5'- TCAGAGGAGGTATGGTTCCCAG -3'
(R):5'- ACCTGCTCCTAATCAGTGGAC -3'

Sequencing Primer
(F):5'- TATGGTTCCCAGCGAGAGG -3'
(R):5'- AATCAGTGGACAGTTGCCTC -3'

Posted On

2015-02-05