Mutagenetix > Incidental Mutation

Incidental Mutation 'R0373:Olfr1225'

30581

Institutional Source

Beutler Lab

Gene Symbol

Olfr1225

Ensembl Gene

ENSMUSG00000101918

Gene Name

olfactory receptor 1225

Synonyms

MOR233-11, GA_x6K02T2Q125-50650037-50649102

MMRRC Submission

038579-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R0373 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

89168380-89174669 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 89170413 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 266 (F266L)

Ref Sequence

ENSEMBL: ENSMUSP00000150915 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000188861] [ENSMUST00000216961] [ENSMUST00000217054]

AlphaFold

Q7TR01

Predicted Effect

probably benign
Transcript: ENSMUST00000099791
AA Change: F266L

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000097379
Gene: ENSMUSG00000075098
AA Change: F266L

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	303	4e-47	PFAM
Pfam:7tm_1	39	304	2.7e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188861
AA Change: F266L

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000140442
Gene: ENSMUSG00000101918
AA Change: F266L

Domain	Start	End	E-Value	Type
low complexity region	24	32	N/A	INTRINSIC
Pfam:7tm_1	39	289	5.5e-24	PFAM
Pfam:7tm_4	138	287	2.3e-35	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216961
AA Change: F266L

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

Predicted Effect

probably benign
Transcript: ENSMUST00000217054
AA Change: F266L

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

Coding Region Coverage

1x: 99.1%
3x: 98.1%
10x: 95.8%
20x: 91.5%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930579F01Rik	A	T	3: 138,173,582 (GRCm38)	L235Q	probably damaging	Het
Adam6b	T	A	12: 113,490,655 (GRCm38)	V364D	probably benign	Het
Akap13	T	C	7: 75,609,929 (GRCm38)	L767P	probably benign	Het
Akap13	T	A	7: 75,730,500 (GRCm38)	S2193T	probably damaging	Het
Anapc11	T	C	11: 120,605,377 (GRCm38)	V69A	probably benign	Het
Ankmy1	C	T	1: 92,896,190 (GRCm38)	R118Q	probably damaging	Het
Ankrd27	T	C	7: 35,638,053 (GRCm38)	S931P	probably benign	Het
Atp6v1c2	G	A	12: 17,288,168 (GRCm38)	R280C	probably damaging	Het
Bbs10	T	A	10: 111,300,052 (GRCm38)	I342N	probably damaging	Het
Calhm2	T	C	19: 47,132,950 (GRCm38)	D260G	possibly damaging	Het
Camk2a	A	G	18: 60,958,238 (GRCm38)	E264G	probably damaging	Het
Ccdc146	T	A	5: 21,319,545 (GRCm38)	M270L	probably benign	Het
Cdc16	A	G	8: 13,779,264 (GRCm38)	T517A	probably benign	Het
Ces1g	T	C	8: 93,331,193 (GRCm38)	H160R	probably benign	Het
Chst4	T	C	8: 110,030,394 (GRCm38)	N196S	probably damaging	Het
Ciz1	A	T	2: 32,367,467 (GRCm38)	N175Y	probably damaging	Het
Cyb5r4	G	A	9: 87,027,040 (GRCm38)	V57I	probably damaging	Het
Cyth3	A	G	5: 143,684,426 (GRCm38)		probably benign	Het
Def6	A	G	17: 28,220,180 (GRCm38)	E255G	probably damaging	Het
Dhtkd1	T	G	2: 5,911,870 (GRCm38)	Q665P	probably damaging	Het
Dsg3	A	C	18: 20,539,747 (GRCm38)	D825A	probably damaging	Het
Eif3m	T	C	2: 105,005,000 (GRCm38)	T242A	probably benign	Het
Emilin3	A	G	2: 160,909,817 (GRCm38)	F101L	probably benign	Het
Epha7	A	G	4: 28,935,700 (GRCm38)		probably null	Het
Fam205a1	T	C	4: 42,851,161 (GRCm38)	I332V	probably benign	Het
Fbxo45	A	T	16: 32,238,405 (GRCm38)	Y224N	probably damaging	Het
Fhod3	A	T	18: 25,090,104 (GRCm38)	M836L	possibly damaging	Het
Fut4	C	A	9: 14,751,210 (GRCm38)	V263F	probably damaging	Het
Ggt1	C	T	10: 75,579,270 (GRCm38)	T206M	probably benign	Het
Gls	T	C	1: 52,188,699 (GRCm38)	R79G	probably damaging	Het
Gm436	A	T	4: 144,686,220 (GRCm38)	M50K	possibly damaging	Het
Grhl1	T	C	12: 24,581,515 (GRCm38)	S156P	probably benign	Het
Ipo8	C	T	6: 148,775,042 (GRCm38)	S983N	probably benign	Het
Kcna7	C	T	7: 45,409,444 (GRCm38)	A385V	probably damaging	Het
Kpnb1	A	T	11: 97,185,090 (GRCm38)	L40Q	probably damaging	Het
Matn1	A	T	4: 130,950,106 (GRCm38)	S209C	probably damaging	Het
Mcc	A	G	18: 44,475,222 (GRCm38)	I501T	probably benign	Het
Mdp1	A	T	14: 55,659,375 (GRCm38)	F104L	probably damaging	Het
Mib2	A	T	4: 155,656,288 (GRCm38)	N626K	probably damaging	Het
Mrgprh	T	C	17: 12,876,956 (GRCm38)	S28P	possibly damaging	Het
Mup-ps23	T	A	4: 61,856,149 (GRCm38)		noncoding transcript	Het
Myh15	A	G	16: 49,182,959 (GRCm38)	T1794A	possibly damaging	Het
Myo18a	C	G	11: 77,821,042 (GRCm38)	P680A	probably benign	Het
Myom2	G	T	8: 15,098,419 (GRCm38)	D532Y	possibly damaging	Het
Ndufaf5	A	G	2: 140,170,881 (GRCm38)	N57S	probably benign	Het
Nectin3	C	T	16: 46,458,187 (GRCm38)	V282M	probably damaging	Het
Nup188	G	T	2: 30,330,988 (GRCm38)	D997Y	probably damaging	Het
Olfm3	T	C	3: 115,122,805 (GRCm38)	V462A	probably damaging	Het
Olfr1044	A	C	2: 86,171,706 (GRCm38)	F37C	probably damaging	Het
Olfr305	A	T	7: 86,363,805 (GRCm38)	C177*	probably null	Het
Opcml	A	G	9: 28,813,398 (GRCm38)	H164R	possibly damaging	Het
Pacrg	A	G	17: 10,403,418 (GRCm38)	I209T	probably damaging	Het
Pcf11	T	C	7: 92,661,215 (GRCm38)	M522V	probably benign	Het
Pck1	T	A	2: 173,153,390 (GRCm38)	M1K	probably null	Het
Pcm1	G	T	8: 41,276,111 (GRCm38)	E707*	probably null	Het
Pcsk5	G	A	19: 17,654,849 (GRCm38)	R318W	probably damaging	Het
Phf11d	A	T	14: 59,353,344 (GRCm38)	M188K	possibly damaging	Het
Ppip5k2	A	T	1: 97,740,537 (GRCm38)	C615*	probably null	Het
Prkdc	T	A	16: 15,791,927 (GRCm38)	S3132T	probably damaging	Het
Prl2c5	A	T	13: 13,183,024 (GRCm38)		probably benign	Het
Prpsap2	A	G	11: 61,741,000 (GRCm38)	I177T	possibly damaging	Het
Rad50	A	G	11: 53,650,519 (GRCm38)	S1297P	probably damaging	Het
Rasip1	T	A	7: 45,635,244 (GRCm38)	N678K	possibly damaging	Het
Rubcn	A	G	16: 32,835,980 (GRCm38)	S544P	probably damaging	Het
Rwdd2a	A	T	9: 86,574,400 (GRCm38)	T210S	possibly damaging	Het
Scd2	A	G	19: 44,303,040 (GRCm38)	D306G	probably damaging	Het
Sema3b	T	C	9: 107,602,918 (GRCm38)	N207S	probably benign	Het
Sf3b2	C	T	19: 5,274,824 (GRCm38)	D845N	probably damaging	Het
Sipa1l2	C	A	8: 125,464,410 (GRCm38)	C947F	probably damaging	Het
Slc12a1	A	T	2: 125,226,031 (GRCm38)	T1013S	probably damaging	Het
Slc18a2	A	T	19: 59,287,367 (GRCm38)	I461L	probably benign	Het
Slc1a6	C	A	10: 78,801,922 (GRCm38)	Y427*	probably null	Het
Slc30a4	A	T	2: 122,689,399 (GRCm38)	I231K	probably damaging	Het
Sos1	G	T	17: 80,453,763 (GRCm38)	A168D	probably damaging	Het
Sptb	T	C	12: 76,621,371 (GRCm38)	S651G	probably benign	Het
Stk36	T	C	1: 74,633,620 (GRCm38)	L1007P	probably damaging	Het
Tek	A	T	4: 94,804,341 (GRCm38)	N229Y	probably damaging	Het
Tep1	A	G	14: 50,836,768 (GRCm38)	F1887L	possibly damaging	Het
Tet1	A	T	10: 62,878,209 (GRCm38)	C602*	probably null	Het
Tnfrsf19	A	G	14: 60,972,036 (GRCm38)	S262P	possibly damaging	Het
Trim5	T	C	7: 104,265,684 (GRCm38)	I393V	probably benign	Het
Trpm6	A	G	19: 18,853,587 (GRCm38)	E1272G	probably benign	Het
Ttc21b	A	T	2: 66,188,326 (GRCm38)	Y1246N	probably damaging	Het
Ttll3	T	A	6: 113,398,777 (GRCm38)	L151H	probably damaging	Het
U2surp	C	T	9: 95,484,443 (GRCm38)	V470I	probably benign	Het
Ubr1	A	T	2: 120,946,657 (GRCm38)	Y276N	probably benign	Het
Uggt1	A	G	1: 36,179,670 (GRCm38)	S59P	probably benign	Het
Unc45a	T	C	7: 80,326,344 (GRCm38)	T796A	probably damaging	Het
Unc5b	C	A	10: 60,778,940 (GRCm38)	V193F	possibly damaging	Het
Upp1	G	T	11: 9,129,590 (GRCm38)	M50I	probably benign	Het
Vps18	C	T	2: 119,293,905 (GRCm38)	R438C	probably damaging	Het
Zfp715	T	C	7: 43,299,336 (GRCm38)	Y400C	possibly damaging	Het
Zfp955b	T	C	17: 33,302,522 (GRCm38)	Y322H	probably benign	Het

Other mutations in Olfr1225

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03230:Olfr1225	APN	2	89,171,089 (GRCm38)	missense	possibly damaging	0.53
IGL03381:Olfr1225	APN	2	89,171,179 (GRCm38)	missense	possibly damaging	0.49
IGL03147:Olfr1225	UTSW	2	89,170,972 (GRCm38)	missense	probably benign	0.03
PIT4458001:Olfr1225	UTSW	2	89,170,633 (GRCm38)	missense	probably benign	0.02
R0100:Olfr1225	UTSW	2	89,171,087 (GRCm38)	missense	probably benign	0.00
R0482:Olfr1225	UTSW	2	89,170,631 (GRCm38)	missense	probably benign	0.37
R0491:Olfr1225	UTSW	2	89,170,360 (GRCm38)	missense	probably benign	0.07
R0548:Olfr1225	UTSW	2	89,170,648 (GRCm38)	missense	probably damaging	1.00
R1123:Olfr1225	UTSW	2	89,170,868 (GRCm38)	missense	possibly damaging	0.89
R1511:Olfr1225	UTSW	2	89,170,937 (GRCm38)	missense	probably damaging	1.00
R1565:Olfr1225	UTSW	2	89,170,627 (GRCm38)	missense	probably benign	0.01
R4204:Olfr1225	UTSW	2	89,170,780 (GRCm38)	missense	probably benign	0.13
R4580:Olfr1225	UTSW	2	89,171,200 (GRCm38)	missense	probably benign	0.01
R4669:Olfr1225	UTSW	2	89,170,901 (GRCm38)	missense	probably damaging	1.00
R5137:Olfr1225	UTSW	2	89,170,400 (GRCm38)	missense	probably benign	0.00
R6391:Olfr1225	UTSW	2	89,170,598 (GRCm38)	missense	probably benign	0.03
R6396:Olfr1225	UTSW	2	89,170,690 (GRCm38)	missense	probably damaging	0.99
R7103:Olfr1225	UTSW	2	89,170,483 (GRCm38)	missense	possibly damaging	0.77
R7187:Olfr1225	UTSW	2	89,171,370 (GRCm38)	start gained	probably benign
R7394:Olfr1225	UTSW	2	89,170,361 (GRCm38)	missense	probably benign
R7758:Olfr1225	UTSW	2	89,171,141 (GRCm38)	missense	probably benign	0.00
R8073:Olfr1225	UTSW	2	89,170,940 (GRCm38)	missense	probably damaging	0.99
R9012:Olfr1225	UTSW	2	89,170,585 (GRCm38)	missense	possibly damaging	0.90
R9632:Olfr1225	UTSW	2	89,171,408 (GRCm38)	start gained	probably benign

Predicted Primers

PCR Primer
(F):5'- TTTGACATGGATGCTTGAAATCACAGC -3'
(R):5'- TGAAGCTTGCCTGCACTGACAC -3'

Sequencing Primer
(F):5'- AGTGCTCTTAGCCAACAGTC -3'
(R):5'- GCCTGCACTGACACCTATATATTTG -3'

Posted On

2013-04-24