|Institutional Source||Beutler Lab|
|Gene Name||argonaute RISC catalytic subunit 3|
|Synonyms||eIF2C3, argonaute 3, C130014L07Rik|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R4079 (G1)|
|Chromosomal Location||126331704-126429556 bp(-) (GRCm38)|
|Type of Mutation||critical splice donor site (1 bp from exon)|
|DNA Base Change (assembly)||C to G at 126353680 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000066633 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000069097] [ENSMUST00000127831] [ENSMUST00000132123]|
|Meta Mutation Damage Score||0.9496|
|Coding Region Coverage||
|Validation Efficiency||97% (73/75)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ago3||
(F):5'- TAGAGACCCAGCTGGAGAAG -3'
(R):5'- ACTGTCAGTGGAGTGCAGTTAT -3'
(F):5'- CCCAGCTGGAGAAGGGAGG -3'
(R):5'- TATTGCAATCATGTGAAACAGGTG -3'