Mutagenetix > Incidental Mutation

Incidental Mutation 'R5428:Gad1-ps'

427023

Institutional Source

Beutler Lab

Gene Symbol

Gad1-ps

Ensembl Gene

ENSMUSG00000090665

Gene Name

glutamate decarboxylase 1, pseudogene

Synonyms

Gad-1ps

MMRRC Submission

042994-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.208) question?

Stock #

R5428 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

99279906-99281681 bp(+) (GRCm39)

Type of Mutation

exon

DNA Base Change (assembly)

G to A at 99281009 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000167243

SMART Domains

Protein: ENSMUSP00000133048
Gene: ENSMUSG00000090665

Domain	Start	End	E-Value	Type
Pfam:Pyridoxal_deC	1	368	4.3e-153	PFAM
Pfam:Beta_elim_lyase	91	436	7.3e-8	PFAM
Pfam:Aminotran_5	130	370	4.7e-7	PFAM

Meta Mutation Damage Score

0.2911

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

98% (62/63)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921524L21Rik	G	C	18: 6,635,918 (GRCm39)	V324L	probably benign	Het
Abl2	T	C	1: 156,469,681 (GRCm39)	C982R	probably damaging	Het
Adgrl4	G	A	3: 151,248,323 (GRCm39)	C665Y	probably damaging	Het
Adra1d	A	G	2: 131,403,323 (GRCm39)	S256P	probably damaging	Het
Aipl1	T	A	11: 71,921,313 (GRCm39)	Y194F	probably benign	Het
Arfgef1	C	A	1: 10,231,060 (GRCm39)	D1150Y	probably damaging	Het
Arl14epl	A	T	18: 47,059,387 (GRCm39)	M1L	possibly damaging	Het
Colgalt1	G	T	8: 72,075,420 (GRCm39)	R442L	probably damaging	Het
Ctnnd1	C	T	2: 84,447,133 (GRCm39)	V371M	probably damaging	Het
Dach1	A	G	14: 98,406,705 (GRCm39)	V14A	unknown	Het
Defa30	A	G	8: 21,625,419 (GRCm39)	D61G	probably benign	Het
Dnaja2	A	T	8: 86,266,804 (GRCm39)	C308S	probably benign	Het
Emc4	A	G	2: 112,197,700 (GRCm39)		probably benign	Het
Foxp1	A	G	6: 98,993,592 (GRCm39)	V104A	probably damaging	Het
Fry	T	G	5: 150,328,824 (GRCm39)	L1319R	possibly damaging	Het
Gja10	T	A	4: 32,601,169 (GRCm39)	H405L	probably benign	Het
Gm27047	A	G	6: 130,607,527 (GRCm39)		noncoding transcript	Het
Grm1	T	A	10: 10,595,307 (GRCm39)	T774S	probably damaging	Het
Gtse1	A	C	15: 85,746,340 (GRCm39)	D52A	probably benign	Het
Kctd17	T	A	15: 78,312,982 (GRCm39)	F42Y	probably damaging	Het
Kndc1	A	G	7: 139,488,878 (GRCm39)	K178E	probably damaging	Het
Lrrc74b	T	C	16: 17,376,125 (GRCm39)	E175G	probably damaging	Het
Macf1	A	G	4: 123,278,661 (GRCm39)	I5927T	probably damaging	Het
Maml2	A	G	9: 13,617,191 (GRCm39)	N935S	probably benign	Het
Man2a1	A	G	17: 65,019,295 (GRCm39)	I720V	probably benign	Het
Map3k5	C	T	10: 19,899,399 (GRCm39)	H219Y	possibly damaging	Het
Map4k5	T	C	12: 69,884,787 (GRCm39)	T314A	possibly damaging	Het
Mast3	A	G	8: 71,237,377 (GRCm39)	V615A	possibly damaging	Het
Mcmbp	C	T	7: 128,306,248 (GRCm39)	V457I	probably benign	Het
Myt1l	G	A	12: 29,882,331 (GRCm39)	G509R	unknown	Het
Nacc1	T	C	8: 85,402,783 (GRCm39)	I337V	probably damaging	Het
Nipbl	T	C	15: 8,359,780 (GRCm39)	D1475G	probably benign	Het
Orc1	T	A	4: 108,457,137 (GRCm39)	D392E	probably benign	Het
Pappa2	T	C	1: 158,642,355 (GRCm39)	T1297A	possibly damaging	Het
Plag1	C	T	4: 3,905,538 (GRCm39)	V51I	possibly damaging	Het
Ppp1r12a	G	A	10: 108,089,208 (GRCm39)	E616K	possibly damaging	Het
Ptgs1	A	G	2: 36,135,280 (GRCm39)	M415V	probably benign	Het
Ralgapa2	C	T	2: 146,176,414 (GRCm39)	E1683K	probably damaging	Het
Rap2a	T	A	14: 120,716,406 (GRCm39)	F90I	probably benign	Het
Rcvrn	A	G	11: 67,590,875 (GRCm39)	E153G	possibly damaging	Het
Sharpin	C	A	15: 76,234,866 (GRCm39)		probably benign	Het
Skil	T	A	3: 31,151,647 (GRCm39)	D56E	probably benign	Het
Slc22a18	G	T	7: 143,033,082 (GRCm39)	G57W	probably damaging	Het
Sntb1	C	G	15: 55,506,191 (GRCm39)	G461R	probably damaging	Het
Synj1	T	C	16: 90,788,406 (GRCm39)	D154G	probably damaging	Het
Tbc1d24	A	T	17: 24,400,746 (GRCm39)	N156K	probably benign	Het
Tcea1	A	T	1: 4,950,568 (GRCm39)		probably benign	Het
Tmc3	T	C	7: 83,261,755 (GRCm39)	V611A	probably damaging	Het
Tnrc6c	A	G	11: 117,591,588 (GRCm39)	M1V	probably null	Het
Tomm5	A	T	4: 45,106,689 (GRCm39)		probably benign	Het
Ttc6	A	G	12: 57,736,620 (GRCm39)	K1207R	probably null	Het
Ttn	T	C	2: 76,591,448 (GRCm39)	T12747A	possibly damaging	Het
Ttn	A	T	2: 76,715,480 (GRCm39)		probably benign	Het
Utrn	T	C	10: 12,569,175 (GRCm39)	D1147G	probably benign	Het
Vmn1r53	T	C	6: 90,200,395 (GRCm39)	I310V	probably benign	Het
Washc4	A	C	10: 83,410,386 (GRCm39)	D658A	probably benign	Het
Wrap73	G	A	4: 154,229,731 (GRCm39)	R34Q	probably damaging	Het
Ylpm1	T	C	12: 85,077,003 (GRCm39)	F1243L	probably benign	Het
Zbtb24	T	C	10: 41,340,784 (GRCm39)	S605P	probably benign	Het
Zfy1	T	G	Y: 726,205 (GRCm39)	H520P	possibly damaging	Het

Other mutations in Gad1-ps

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL00963:Gad1-ps	APN	10	99,281,310 (GRCm39)	exon	noncoding transcript
IGL01301:Gad1-ps	APN	10	99,281,013 (GRCm39)	exon	noncoding transcript
IGL01394:Gad1-ps	APN	10	99,281,424 (GRCm39)	exon	noncoding transcript
IGL02220:Gad1-ps	APN	10	99,281,184 (GRCm39)	exon	noncoding transcript
IGL02240:Gad1-ps	APN	10	99,280,820 (GRCm39)	exon	noncoding transcript
IGL03406:Gad1-ps	APN	10	99,280,641 (GRCm39)	exon	noncoding transcript
ANU18:Gad1-ps	UTSW	10	99,281,013 (GRCm39)	exon	noncoding transcript
R0305:Gad1-ps	UTSW	10	99,280,665 (GRCm39)	exon	noncoding transcript
R0446:Gad1-ps	UTSW	10	99,281,383 (GRCm39)	exon	noncoding transcript
R0538:Gad1-ps	UTSW	10	99,280,854 (GRCm39)	exon	noncoding transcript
R1511:Gad1-ps	UTSW	10	99,281,331 (GRCm39)	exon	noncoding transcript
R1734:Gad1-ps	UTSW	10	99,281,637 (GRCm39)	exon	noncoding transcript
R1745:Gad1-ps	UTSW	10	99,281,386 (GRCm39)	exon	noncoding transcript
R1886:Gad1-ps	UTSW	10	99,281,444 (GRCm39)	exon	noncoding transcript
R3111:Gad1-ps	UTSW	10	99,280,383 (GRCm39)	exon	noncoding transcript
R3617:Gad1-ps	UTSW	10	99,281,260 (GRCm39)	exon	noncoding transcript
R5042:Gad1-ps	UTSW	10	99,281,516 (GRCm39)	exon	noncoding transcript
R5223:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5234:Gad1-ps	UTSW	10	99,281,188 (GRCm39)	exon	noncoding transcript
R5275:Gad1-ps	UTSW	10	99,280,751 (GRCm39)	exon	noncoding transcript
R5295:Gad1-ps	UTSW	10	99,280,751 (GRCm39)	exon	noncoding transcript
R5334:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5335:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5336:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5337:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5396:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5397:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5399:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5429:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5431:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5661:Gad1-ps	UTSW	10	99,280,901 (GRCm39)	exon	noncoding transcript
R5667:Gad1-ps	UTSW	10	99,280,395 (GRCm39)	exon	noncoding transcript
R5671:Gad1-ps	UTSW	10	99,280,395 (GRCm39)	exon	noncoding transcript
R5885:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript
R5886:Gad1-ps	UTSW	10	99,281,009 (GRCm39)	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- TGCTCTTGGCTTTGGAACAG -3'
(R):5'- TGCACACATCTGGCTGCATC -3'

Sequencing Primer
(F):5'- CTCTTGGCTTTGGAACAGACAATG -3'
(R):5'- ACATCTGGCTGCATCCTTGGAG -3'

Posted On

2016-09-01