Mutagenetix > Incidental Mutations

Incidental Mutation 'R6312:Zfp60'

509813

Institutional Source

Beutler Lab

Gene Symbol

Zfp60

Ensembl Gene

ENSMUSG00000037640

Gene Name

zinc finger protein 60

Synonyms

6330516O17Rik, Mfg-3, Mfg3

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6312 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

27731389-27753702 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 27748776 bp

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 290 (C290R)

Ref Sequence

ENSEMBL: ENSMUSP00000132607 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042641] [ENSMUST00000108336] [ENSMUST00000130997] [ENSMUST00000136373] [ENSMUST00000167955]

Predicted Effect

probably damaging
Transcript: ENSMUST00000042641
AA Change: C290R

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000036627
Gene: ENSMUSG00000037640
AA Change: C290R

Domain	Start	End	E-Value	Type
KRAB	14	75	8.48e-36	SMART
ZnF_C2H2	173	195	2.67e-1	SMART
ZnF_C2H2	201	223	2.09e-3	SMART
ZnF_C2H2	229	251	1.18e-2	SMART
ZnF_C2H2	257	279	2.24e-3	SMART
ZnF_C2H2	285	307	2.65e-5	SMART
ZnF_C2H2	313	335	5.5e-3	SMART
ZnF_C2H2	341	363	3.89e-3	SMART
ZnF_C2H2	369	391	6.32e-3	SMART
ZnF_C2H2	397	419	5.14e-3	SMART
ZnF_C2H2	425	447	7.9e-4	SMART
ZnF_C2H2	453	475	1.67e-2	SMART
ZnF_C2H2	481	503	4.4e-2	SMART
ZnF_C2H2	509	531	4.24e-4	SMART
ZnF_C2H2	537	559	5.21e-4	SMART
ZnF_C2H2	565	587	5.14e-3	SMART
ZnF_C2H2	593	615	3.89e-3	SMART
ZnF_C2H2	621	643	1.95e-3	SMART
ZnF_C2H2	649	671	6.67e-2	SMART
ZnF_C2H2	677	699	8.34e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000108336
AA Change: C290R

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000103973
Gene: ENSMUSG00000037640
AA Change: C290R

Domain	Start	End	E-Value	Type
KRAB	14	75	8.48e-36	SMART
ZnF_C2H2	173	195	2.67e-1	SMART
ZnF_C2H2	201	223	2.09e-3	SMART
ZnF_C2H2	229	251	1.18e-2	SMART
ZnF_C2H2	257	279	2.24e-3	SMART
ZnF_C2H2	285	307	2.65e-5	SMART
ZnF_C2H2	313	335	5.5e-3	SMART
ZnF_C2H2	341	363	3.89e-3	SMART
ZnF_C2H2	369	391	6.32e-3	SMART
ZnF_C2H2	397	419	5.14e-3	SMART
ZnF_C2H2	425	447	7.9e-4	SMART
ZnF_C2H2	453	475	1.67e-2	SMART
ZnF_C2H2	481	503	4.4e-2	SMART
ZnF_C2H2	509	531	4.24e-4	SMART
ZnF_C2H2	537	559	5.21e-4	SMART
ZnF_C2H2	565	587	5.14e-3	SMART
ZnF_C2H2	593	615	3.89e-3	SMART
ZnF_C2H2	621	643	1.95e-3	SMART
ZnF_C2H2	649	671	6.67e-2	SMART
ZnF_C2H2	677	699	8.34e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000130997

SMART Domains

Protein: ENSMUSP00000118469
Gene: ENSMUSG00000037640

Domain	Start	End	E-Value	Type
KRAB	14	75	8.48e-36	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000136373

SMART Domains

Protein: ENSMUSP00000117049
Gene: ENSMUSG00000037640

Domain	Start	End	E-Value	Type
KRAB	14	55	3.2e-13	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000167955
AA Change: C290R

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000132607
Gene: ENSMUSG00000037640
AA Change: C290R

Domain	Start	End	E-Value	Type
KRAB	14	75	8.48e-36	SMART
ZnF_C2H2	173	195	2.67e-1	SMART
ZnF_C2H2	201	223	2.09e-3	SMART
ZnF_C2H2	229	251	1.18e-2	SMART
ZnF_C2H2	257	279	2.24e-3	SMART
ZnF_C2H2	285	307	2.65e-5	SMART
ZnF_C2H2	313	335	5.5e-3	SMART
ZnF_C2H2	341	363	3.89e-3	SMART
ZnF_C2H2	369	391	6.32e-3	SMART
ZnF_C2H2	397	419	5.14e-3	SMART
ZnF_C2H2	425	447	7.9e-4	SMART
ZnF_C2H2	453	475	1.67e-2	SMART
ZnF_C2H2	481	503	4.4e-2	SMART
ZnF_C2H2	509	531	4.24e-4	SMART
ZnF_C2H2	537	559	5.21e-4	SMART
ZnF_C2H2	565	587	5.14e-3	SMART
ZnF_C2H2	593	615	3.89e-3	SMART
ZnF_C2H2	621	643	1.95e-3	SMART
ZnF_C2H2	649	671	6.67e-2	SMART
ZnF_C2H2	677	699	8.34e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930447C04Rik	A	G	12: 72,889,767	S472P	possibly damaging	Het
9430007A20Rik	A	T	4: 144,528,502	H164L	probably benign	Het
Abraxas2	G	A	7: 132,874,965	A145T	probably damaging	Het
AC153874.1	T	A	10: 77,847,127		probably benign	Het
Acadvl	T	A	11: 70,011,767	M375L	probably damaging	Het
Ankdd1a	C	T	9: 65,508,061	A227T	possibly damaging	Het
Arl4d	T	C	11: 101,667,253	*202R	probably null	Het
B3gat2	G	T	1: 23,815,467	E83*	probably null	Het
BC017158	T	C	7: 128,273,543	K411R	probably benign	Het
Bmper	C	A	9: 23,406,791	Q569K	possibly damaging	Het
C2cd4d	C	A	3: 94,364,435	P336H	probably damaging	Het
Cct2	A	T	10: 117,056,055	S363T	probably benign	Het
Cers5	A	G	15: 99,747,115	V119A	probably benign	Het
Cfhr2	C	T	1: 139,831,079	V78I	possibly damaging	Het
Crocc2	A	T	1: 93,215,710	K1345*	probably null	Het
Cyp4f39	T	C	17: 32,483,294	M255T	probably benign	Het
Dpp6	A	T	5: 27,725,671	I834F	possibly damaging	Het
Dpy19l4	T	A	4: 11,289,671	K205*	probably null	Het
Epg5	T	A	18: 77,979,211	D1056E	possibly damaging	Het
Fam20a	A	C	11: 109,674,630	C452G	probably damaging	Het
Gnai2	A	T	9: 107,635,117	V34E	probably damaging	Het
Gng3	A	G	19: 8,838,633	V7A	probably benign	Het
Hdc	A	G	2: 126,607,406	V77A	possibly damaging	Het
Hint1	G	A	11: 54,869,990	C85Y	probably benign	Het
Kif17	C	T	4: 138,288,193	S551L	probably benign	Het
Lgr5	A	G	10: 115,452,924	L581P	probably damaging	Het
Lig4	G	T	8: 9,971,739	N680K	probably benign	Het
Lipi	T	A	16: 75,573,915	Y138F	probably damaging	Het
Lrp2	C	T	2: 69,436,681	G4294E	probably damaging	Het
Lrrc7	A	G	3: 158,160,609	M1165T	probably benign	Het
Mtpap	T	C	18: 4,396,175	I489T	possibly damaging	Het
Nlrp1b	T	A	11: 71,228,397	N24I	probably benign	Het
Nlrp4a	A	G	7: 26,449,396	T143A	probably benign	Het
Nudt2	A	G	4: 41,480,386	T90A	probably benign	Het
Olfr1055	A	T	2: 86,347,581	F62I	probably damaging	Het
Olfr352	A	T	2: 36,870,465	I300L	probably benign	Het
Olfr659	T	A	7: 104,671,589	Y296N	probably damaging	Het
Olfr883	ATTGCTGTTT	ATTGCTGTTTGCTGTTT	9: 38,026,540		probably null	Het
Olfr883	TTGCTGT	TTGCTGTCTGCTGT	9: 38,026,541		probably null	Het
Olfr883	GTTT	GTTTGCTGTTTT	9: 38,026,546		probably null	Het
Olfr883	TTT	TTTGCTGATT	9: 38,026,547		probably null	Het
Olfr883	T	TGCTGTTC	9: 38,026,549		probably null	Het
Osmr	A	G	15: 6,823,638	V592A	probably damaging	Het
Rbp2	G	T	9: 98,490,647	S13I	probably benign	Het
Rsf1	A	AGGGCGACGG	7: 97,579,904		probably null	Het
Slc6a7	A	G	18: 61,002,385	S381P	probably benign	Het
Slitrk6	A	G	14: 110,750,247	L676P	probably benign	Het
Sspo	T	C	6: 48,457,366		probably null	Het
Tectb	CT	C	19: 55,192,662		probably null	Homo
Tma16	T	C	8: 66,481,466	E79G	probably damaging	Het
Trim14	G	T	4: 46,507,257	H320N	probably damaging	Het
Trim63	A	G	4: 134,325,697	D323G	probably damaging	Het
Vash2	C	A	1: 190,958,683	R309L	probably benign	Het
Vmn1r62	G	A	7: 5,676,084	V255M	possibly damaging	Het
Vmn2r53	T	A	7: 12,598,639		probably null	Het
Zfp382	G	A	7: 30,134,538	R538H	probably damaging	Het
Zfp592	T	A	7: 81,023,436	D49E	probably benign	Het
Zfp69	G	A	4: 120,949,517		probably benign	Het
Zfp790	G	T	7: 29,828,222	G111W	probably damaging	Het
Zfp948	T	C	17: 21,587,167	I207T	possibly damaging	Het

Other mutations in Zfp60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03353:Zfp60	APN	7	27748334	nonsense	probably null
PIT4494001:Zfp60	UTSW	7	27748701	missense	probably damaging	1.00
R1281:Zfp60	UTSW	7	27738427	missense	probably damaging	1.00
R1643:Zfp60	UTSW	7	27736975	missense	probably damaging	0.98
R1883:Zfp60	UTSW	7	27750010	missense	probably benign	0.00
R1930:Zfp60	UTSW	7	27736957	start codon destroyed	probably null	1.00
R3623:Zfp60	UTSW	7	27749328	missense	probably benign	0.01
R3624:Zfp60	UTSW	7	27749328	missense	probably benign	0.01
R3875:Zfp60	UTSW	7	27749581	missense	probably damaging	1.00
R5296:Zfp60	UTSW	7	27738530	intron	probably benign
R5491:Zfp60	UTSW	7	27748515	splice site	probably null
R5724:Zfp60	UTSW	7	27748333	missense	probably benign
R6134:Zfp60	UTSW	7	27749898	missense	probably benign	0.01
R6477:Zfp60	UTSW	7	27749803	missense	probably benign	0.00
R6649:Zfp60	UTSW	7	27748726	missense	probably benign	0.00
R6653:Zfp60	UTSW	7	27748726	missense	probably benign	0.00
R7041:Zfp60	UTSW	7	27749026	missense	probably benign	0.05
R7166:Zfp60	UTSW	7	27749512	missense	possibly damaging	0.82
R7185:Zfp60	UTSW	7	27738405	missense	probably damaging	1.00
R7334:Zfp60	UTSW	7	27749019	missense	probably damaging	1.00
R7560:Zfp60	UTSW	7	27749136	missense	probably damaging	0.99
R7560:Zfp60	UTSW	7	27749271	missense	probably damaging	1.00
R7561:Zfp60	UTSW	7	27748530	missense	probably damaging	1.00
R7673:Zfp60	UTSW	7	27748315	missense	probably benign	0.00
R7961:Zfp60	UTSW	7	27748456	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GTCTACTTACACTAGCAAGACTCTTG -3'
(R):5'- TTACCACACTGCTTACATTCAAACG -3'

Sequencing Primer
(F):5'- ACTGTGGGAAGACCTTCAGACTC -3'
(R):5'- TCAAACGATTTGTCTTCAGTGTG -3'

Posted On

2018-04-02