Mutagenetix > Incidental Mutations

Incidental Mutation 'R6477:Olfr1154'

516825

Institutional Source

Beutler Lab

Gene Symbol

Olfr1154

Ensembl Gene

ENSMUSG00000075146

Gene Name

olfactory receptor 1154

Synonyms

GA_x6K02T2Q125-49403456-49402524, MOR173-2

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R6477 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

87901256-87905166 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 87902990 bp

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 229 (S229P)

Ref Sequence

ENSEMBL: ENSMUSP00000148909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099844] [ENSMUST00000215017] [ENSMUST00000215862] [ENSMUST00000217436]

Predicted Effect

probably damaging
Transcript: ENSMUST00000099844
AA Change: S229P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000097432
Gene: ENSMUSG00000075146
AA Change: S229P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	8.1e-47	PFAM
Pfam:7tm_1	41	289	3.6e-18	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215017
AA Change: S229P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215862
AA Change: S229P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000217436

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.8%
20x: 93.1%

Validation Efficiency

97% (60/62)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931406P16Rik	A	G	7: 34,257,630		probably null	Het
4933415F23Rik	A	T	1: 23,101,780	L151Q	probably benign	Het
Aatk	T	C	11: 120,018,870	D23G	probably benign	Het
Abca14	T	C	7: 120,325,102	I1659T	probably benign	Het
Ankdd1a	C	T	9: 65,502,212	V481M	probably benign	Het
Ankfy1	G	A	11: 72,730,482	R198Q	possibly damaging	Het
Cfap161	T	A	7: 83,794,022	R20*	probably null	Het
Cops7a	A	T	6: 124,960,176	V184E	probably damaging	Het
Cpa3	T	C	3: 20,239,575	E83G	possibly damaging	Het
Crls1	C	T	2: 132,861,233	S201L	probably benign	Het
Cyp4f39	C	A	17: 32,481,817	S153R	probably damaging	Het
Ddx54	T	A	5: 120,621,778	I410N	probably damaging	Het
Dido1	T	A	2: 180,660,481	T1877S	probably benign	Het
Dip2c	A	G	13: 9,623,760	S1079G	probably damaging	Het
Dnhd1	G	A	7: 105,677,886	V681I	probably benign	Het
Dst	A	G	1: 34,208,728		probably null	Het
Eya2	A	G	2: 165,763,761	T362A	probably benign	Het
Hgs	G	A	11: 120,469,655	V60M	probably damaging	Het
Inpp4b	A	G	8: 81,844,714		probably null	Het
Iqub	T	A	6: 24,449,745	N707I	probably damaging	Het
Kdr	C	A	5: 75,968,841	A129S	probably benign	Het
Lhcgr	A	T	17: 88,742,373	M575K	probably damaging	Het
Lsamp	G	T	16: 42,168,165		probably benign	Het
Mcm5	T	C	8: 75,112,602	V161A	probably benign	Het
Mmp14	A	T	14: 54,437,658	H249L	probably damaging	Het
Mroh5	A	G	15: 73,790,755	S405P	probably damaging	Het
Mroh9	C	A	1: 163,076,304	L46F	probably damaging	Het
Mtrr	A	T	13: 68,570,073	H357Q	probably damaging	Het
Myrf	G	A	19: 10,228,785	P89L	probably benign	Het
Mzb1	C	T	18: 35,648,258		probably null	Het
Naip6	A	G	13: 100,316,008	S182P	probably damaging	Het
Nmur2	A	T	11: 56,029,591	F276Y	probably damaging	Het
Nudt12	G	T	17: 59,011,145	S35Y	probably benign	Het
Olfr572	T	A	7: 102,928,378	V250E	probably damaging	Het
Olfr657	T	C	7: 104,635,679	S2P	probably benign	Het
Osbpl1a	T	C	18: 12,756,261	I541V	probably benign	Het
Parp4	A	G	14: 56,647,237	K1258E	probably benign	Het
Plcxd2	C	G	16: 45,980,659	K67N	probably damaging	Het
Pnpla6	A	G	8: 3,536,627	T930A	probably benign	Het
Pomt1	G	A	2: 32,248,716		probably null	Het
Pramel6	A	G	2: 87,510,602	Y426C	possibly damaging	Het
Prss27	A	G	17: 24,044,261	T83A	probably damaging	Het
Rbm27	T	A	18: 42,333,318	V915E	probably damaging	Het
Rnf114	T	A	2: 167,503,488	D10E	probably benign	Het
Sbk1	A	G	7: 126,291,178	E121G	probably damaging	Het
Senp6	A	T	9: 80,093,625	R39*	probably null	Het
Sergef	A	T	7: 46,633,826	I94N	probably benign	Het
Slc30a9	C	T	5: 67,328,524	R183W	probably benign	Het
Smurf1	C	T	5: 144,889,792	R414H	possibly damaging	Het
Sptb	A	T	12: 76,606,392	W1566R	probably damaging	Het
Tenm2	T	C	11: 36,010,507		probably null	Het
Tnni1	T	C	1: 135,805,566	V42A	probably benign	Het
Tnxb	A	G	17: 34,719,539	Y3098C	probably damaging	Het
Ubald2	T	C	11: 116,434,574	F46L	probably benign	Het
Ubqln5	G	A	7: 104,128,258	S453F	probably damaging	Het
Ubr3	A	T	2: 69,979,429	R1248*	probably null	Het
Utp20	A	G	10: 88,768,918	V1705A	probably benign	Het
Zfp60	G	A	7: 27,749,803	R632H	probably benign	Het
Zfp638	A	G	6: 83,965,578	Y957C	probably damaging	Het
Zfp825	A	T	13: 74,480,910	S162R	possibly damaging	Het
Zfp831	A	C	2: 174,704,167	K1355T	probably benign	Het

Other mutations in Olfr1154

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Olfr1154	APN	2	87903527	missense	probably benign	0.00
IGL01878:Olfr1154	APN	2	87903331	nonsense	probably null
IGL02683:Olfr1154	APN	2	87903104	missense	possibly damaging	0.80
IGL02836:Olfr1154	APN	2	87903380	missense	possibly damaging	0.81
R0432:Olfr1154	UTSW	2	87902960	missense	probably damaging	1.00
R1123:Olfr1154	UTSW	2	87902904	missense	probably damaging	0.99
R1223:Olfr1154	UTSW	2	87902819	missense	probably damaging	0.99
R1561:Olfr1154	UTSW	2	87903161	missense	probably benign	0.00
R1964:Olfr1154	UTSW	2	87903667	missense	probably benign	0.00
R2041:Olfr1154	UTSW	2	87902797	missense	probably damaging	1.00
R2219:Olfr1154	UTSW	2	87902925	nonsense	probably null
R2233:Olfr1154	UTSW	2	87903475	missense	probably damaging	1.00
R3719:Olfr1154	UTSW	2	87903103	missense	probably benign	0.05
R4826:Olfr1154	UTSW	2	87903349	missense	probably damaging	1.00
R4908:Olfr1154	UTSW	2	87903189	missense	probably damaging	1.00
R5056:Olfr1154	UTSW	2	87903571	missense	probably damaging	1.00
R5589:Olfr1154	UTSW	2	87903347	missense	probably benign	0.26
R6532:Olfr1154	UTSW	2	87903202	missense	probably damaging	1.00
R6666:Olfr1154	UTSW	2	87903508	missense	probably damaging	1.00
R6693:Olfr1154	UTSW	2	87903308	missense	probably damaging	1.00
R6724:Olfr1154	UTSW	2	87903602	missense	probably benign	0.00
R7784:Olfr1154	UTSW	2	87903193	missense	probably benign	0.01
Z1088:Olfr1154	UTSW	2	87903584	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- ATTTGACGTCTTTGTTCCTGAGAC -3'
(R):5'- CCAATTGTCATTCTGCTCCAAAG -3'

Sequencing Primer
(F):5'- TCCATTGAATGGCCTGT -3'
(R):5'- GTCATTCTGCTCCAAAGTAATTAACC -3'

Posted On

2018-05-21