Mutagenetix > Incidental Mutation

Incidental Mutation 'R8065:Zfp286'

619955

Institutional Source

Beutler Lab

Gene Symbol

Zfp286

Ensembl Gene

ENSMUSG00000047342

Gene Name

zinc finger protein 286

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R8065 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

62752577-62789462 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 62753519 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Lysine at position 192 (I192K)

Ref Sequence

ENSEMBL: ENSMUSP00000147022 (fasta)

AlphaFold

Q8C0E6

Predicted Effect

probably benign
Transcript: ENSMUST00000178750

Predicted Effect

unknown
Transcript: ENSMUST00000207597
AA Change: I192K

Meta Mutation Damage Score

0.0852

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (47/47)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Cap2	T	C	13: 46,637,861	V280A	probably damaging	Het
Cbx7	A	T	15: 79,933,898	V1D	unknown	Het
Chodl	A	T	16: 78,946,713	L229F	probably damaging	Het
Depdc5	C	A	5: 32,895,908	N197K	possibly damaging	Het
Diaph3	A	T	14: 87,037,495	L175Q	probably damaging	Het
Dlat	A	T	9: 50,657,849	M218K	possibly damaging	Het
Dnaic1	T	A	4: 41,614,258	D311E	probably damaging	Het
Dpp10	A	G	1: 123,352,660	S646P	probably benign	Het
Ebf1	G	A	11: 44,620,547	V90M	probably benign	Het
Efhd1	C	T	1: 87,264,591	P48S	probably benign	Het
Fbxl16	G	A	17: 25,817,983	V313I	probably damaging	Het
Flrt2	A	G	12: 95,780,774	T629A	probably benign	Het
Gpr87	T	A	3: 59,179,887	I66F	probably damaging	Het
Hmmr	G	A	11: 40,721,672	S206F	probably damaging	Het
Hsd17b4	A	T	18: 50,170,752	I431F	possibly damaging	Het
Iba57	T	C	11: 59,163,260		probably benign	Het
Ibtk	A	C	9: 85,720,863	S696R	probably benign	Het
Igkv11-125	C	A	6: 67,913,830	T44N	probably benign	Het
Itih2	T	A	2: 10,123,483	I136F	probably damaging	Het
Itpr3	T	A	17: 27,110,862	D1543E	probably benign	Het
Ldlr	T	A	9: 21,737,945	C339S	probably damaging	Het
Myh2	G	A	11: 67,181,344	E633K	probably null	Het
Myl10	G	C	5: 136,697,971	V70L	probably benign	Het
Mylk	A	T	16: 34,972,019	E1570V	probably benign	Het
Myo15b	G	A	11: 115,887,943		probably null	Het
N4bp2	T	A	5: 65,807,296	L896H	probably damaging	Het
Naip2	A	T	13: 100,189,222	S59R	probably damaging	Het
Ndc1	T	A	4: 107,390,398	S468T	probably benign	Het
Ndst3	T	C	3: 123,601,445	N512S	probably damaging	Het
Olfr1138	T	A	2: 87,737,803	I174F	probably damaging	Het
Olfr65	A	G	7: 103,906,403		probably benign	Het
Plekhn1	T	C	4: 156,228,240	I54V	possibly damaging	Het
Polr3c	T	C	3: 96,715,652	E350G	probably null	Het
Pskh1	T	G	8: 105,929,855	S388A	possibly damaging	Het
Pum1	T	C	4: 130,751,525	V486A	possibly damaging	Het
Rin2	T	G	2: 145,861,057	S558A	probably damaging	Het
Ripk4	A	T	16: 97,763,537	V58D	probably damaging	Het
Slc17a3	A	G	13: 23,858,087	R491G	unknown	Het
Smyd3	A	G	1: 179,410,463	M113T	possibly damaging	Het
Ssh2	A	G	11: 77,441,985	R431G	probably damaging	Het
Timm22	G	A	11: 76,414,105	D190N	probably damaging	Het
Ube2g1	G	A	11: 72,677,765	G103D	probably benign	Het
Zbtb5	A	T	4: 44,994,972	S137R	probably benign	Het
Zfp105	A	G	9: 122,925,129	T8A	probably benign	Het
Zfp942	T	C	17: 21,930,410	Y38C	probably damaging	Het
Zfpm1	T	C	8: 122,335,584	S461P	probably benign	Het

Other mutations in Zfp286

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02659:Zfp286	APN	11	62783737	missense	possibly damaging	0.54
IGL02745:Zfp286	APN	11	62780874	missense	probably damaging	1.00
IGL02826:Zfp286	APN	11	62787960	missense	probably damaging	0.99
R0233:Zfp286	UTSW	11	62780393	missense	possibly damaging	0.75
R0233:Zfp286	UTSW	11	62780393	missense	possibly damaging	0.75
R0318:Zfp286	UTSW	11	62784962	missense	probably damaging	1.00
R1954:Zfp286	UTSW	11	62783708	missense	possibly damaging	0.46
R1994:Zfp286	UTSW	11	62779820	missense	probably damaging	1.00
R2186:Zfp286	UTSW	11	62780461	missense	probably damaging	0.97
R4258:Zfp286	UTSW	11	62781070	missense	probably benign	0.07
R4327:Zfp286	UTSW	11	62780018	missense	probably damaging	1.00
R4453:Zfp286	UTSW	11	62780204	missense	probably damaging	1.00
R4479:Zfp286	UTSW	11	62780204	missense	probably damaging	1.00
R4647:Zfp286	UTSW	11	62783733	nonsense	probably null
R4667:Zfp286	UTSW	11	62780602	missense	probably benign	0.00
R4883:Zfp286	UTSW	11	62780629	missense	probably benign	0.01
R4978:Zfp286	UTSW	11	62788928	critical splice donor site	probably null
R5120:Zfp286	UTSW	11	62780725	missense	probably benign	0.40
R5533:Zfp286	UTSW	11	62780970	intron	probably benign
R7236:Zfp286	UTSW	11	62783670	critical splice donor site	probably null
R7464:Zfp286	UTSW	11	62780801	missense	probably benign	0.00
R8067:Zfp286	UTSW	11	62753519	missense	unknown
R8716:Zfp286	UTSW	11	62780991	missense	unknown
R9351:Zfp286	UTSW	11	62779975	missense	probably damaging	0.97
Z1186:Zfp286	UTSW	11	62784956	missense	probably damaging	1.00
Z1186:Zfp286	UTSW	11	62787969	missense	probably benign
Z1187:Zfp286	UTSW	11	62784956	missense	probably damaging	1.00
Z1187:Zfp286	UTSW	11	62787969	missense	probably benign
Z1188:Zfp286	UTSW	11	62784956	missense	probably damaging	1.00
Z1188:Zfp286	UTSW	11	62787969	missense	probably benign
Z1189:Zfp286	UTSW	11	62784956	missense	probably damaging	1.00
Z1189:Zfp286	UTSW	11	62787969	missense	probably benign
Z1190:Zfp286	UTSW	11	62784956	missense	probably damaging	1.00
Z1190:Zfp286	UTSW	11	62787969	missense	probably benign
Z1191:Zfp286	UTSW	11	62784956	missense	probably damaging	1.00
Z1191:Zfp286	UTSW	11	62787969	missense	probably benign
Z1192:Zfp286	UTSW	11	62784956	missense	probably damaging	1.00
Z1192:Zfp286	UTSW	11	62787969	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CCAAATGGGTATCTGCAATCAAATG -3'
(R):5'- CCCCAGGTTAGGACATAAAAGC -3'

Sequencing Primer
(F):5'- GTAAATTCTTCGTGGAGATTCCC -3'
(R):5'- CCAGGTTAGGACATAAAAGCAAGTTG -3'

Posted On

2020-01-23