Mutagenetix > Incidental Mutation

Incidental Mutation 'R9248:Apob'

701379

Institutional Source

Beutler Lab

Gene Symbol

Apob

Ensembl Gene

ENSMUSG00000020609

Gene Name

apolipoprotein B

Synonyms

apob-100, apob-48

MMRRC Submission

Accession Numbers

Essential gene?

Probably essential (E-score: 0.859) question?

Stock #

R9248 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

8027648-8066835 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 8065231 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 4067 (Q4067*)

Ref Sequence

ENSEMBL: ENSMUSP00000036044 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037520] [ENSMUST00000037811] [ENSMUST00000171239]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000037520
AA Change: Q4034*

SMART Domains

Protein: ENSMUSP00000035761
Gene: ENSMUSG00000020609
AA Change: Q4034*

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	33	585	6.03e-94	SMART
DUF1943	619	932	7.88e-97	SMART
Pfam:DUF1081	945	1059	9.4e-32	PFAM
low complexity region	1100	1109	N/A	INTRINSIC
Blast:LPD_N	1249	1311	9e-22	BLAST
low complexity region	1632	1644	N/A	INTRINSIC
internal_repeat_1	1882	2038	6.61e-9	PROSPERO
SCOP:d1gw5a_	2105	2577	9e-5	SMART
internal_repeat_1	2973	3150	6.61e-9	PROSPERO
low complexity region	3561	3580	N/A	INTRINSIC
low complexity region	3928	3936	N/A	INTRINSIC
Pfam:ApoB100_C	4401	4456	5.6e-34	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000037811
AA Change: Q4067*

SMART Domains

Protein: ENSMUSP00000036044
Gene: ENSMUSG00000020609
AA Change: Q4067*

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	46	598	6.03e-94	SMART
DUF1943	632	945	7.88e-97	SMART
Pfam:DUF1081	960	1070	6.3e-39	PFAM
low complexity region	1113	1122	N/A	INTRINSIC
Blast:LPD_N	1282	1344	1e-21	BLAST
low complexity region	1665	1677	N/A	INTRINSIC
internal_repeat_1	1915	2071	6.6e-9	PROSPERO
SCOP:d1gw5a_	2138	2610	9e-5	SMART
internal_repeat_1	3006	3183	6.6e-9	PROSPERO
low complexity region	3594	3613	N/A	INTRINSIC
low complexity region	3961	3969	N/A	INTRINSIC
Pfam:ApoB100_C	4434	4490	1.6e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171239

SMART Domains

Protein: ENSMUSP00000129496
Gene: ENSMUSG00000020609

Domain	Start	End	E-Value	Type
low complexity region	348	356	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

100% (55/55)

MGI Phenotype

FUNCTION: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mutants usually die by midgestation and longer survivors exhibit exencephaly. Heterozygotes show reduced plasma cholesterol and apolipoprotein levels. Single isoform B100 and B48 null mutants are viable. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acaa1b	T	C	9: 118,983,002 (GRCm39)	N132S	probably benign	Het
Agpat2	A	G	2: 26,483,601 (GRCm39)	*279Q	probably null	Het
Akr1c19	C	A	13: 4,292,974 (GRCm39)	D243E	probably benign	Het
Alg2	A	G	4: 47,474,001 (GRCm39)	F96L	probably benign	Het
C1ra	A	T	6: 124,489,580 (GRCm39)		probably benign	Het
C8a	T	C	4: 104,703,199 (GRCm39)	Y330C	probably damaging	Het
Ccdc185	A	G	1: 182,576,221 (GRCm39)	V156A	probably benign	Het
Ces1g	A	G	8: 94,060,319 (GRCm39)	L100P	possibly damaging	Het
Cnbd1	T	C	4: 18,862,113 (GRCm39)	N359S	possibly damaging	Het
Crybb2	C	T	5: 113,211,094 (GRCm39)	A65T	probably benign	Het
Dcaf5	T	C	12: 80,386,563 (GRCm39)	D521G	probably benign	Het
Dnmt1	A	G	9: 20,833,408 (GRCm39)	F631L	possibly damaging	Het
Dok1	T	A	6: 83,008,893 (GRCm39)	D263V	possibly damaging	Het
Ecel1	A	G	1: 87,081,112 (GRCm39)	F293L	probably benign	Het
Ehmt1	A	T	2: 24,738,077 (GRCm39)	L509Q	possibly damaging	Het
Fbln2	T	A	6: 91,231,556 (GRCm39)	V551E	possibly damaging	Het
Fig4	C	T	10: 41,153,478 (GRCm39)	V108I	probably benign	Het
Gpa33	A	G	1: 165,991,396 (GRCm39)	Y209C	probably damaging	Het
Heatr5a	T	C	12: 51,963,026 (GRCm39)	H52R		Het
Heatr9	A	T	11: 83,409,281 (GRCm39)	D157E	possibly damaging	Het
Jakmip2	A	C	18: 43,685,242 (GRCm39)	M682R	probably benign	Het
Krt1	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	15: 101,758,813 (GRCm39)		probably benign	Het
Layn	A	G	9: 50,968,760 (GRCm39)	S328P	possibly damaging	Het
Mblac2	A	G	13: 81,859,769 (GRCm39)	D41G	probably damaging	Het
Mboat1	A	G	13: 30,410,392 (GRCm39)	Y283C	probably damaging	Het
Mdga2	A	G	12: 66,736,226 (GRCm39)	V334A	possibly damaging	Het
Mroh4	C	T	15: 74,485,167 (GRCm39)	R515H	possibly damaging	Het
Nicn1	C	T	9: 108,171,708 (GRCm39)	R163C	possibly damaging	Het
Nos1	G	C	5: 118,017,402 (GRCm39)	R255P	probably benign	Het
Nsrp1	G	A	11: 76,937,036 (GRCm39)	R387W	probably benign	Het
Or5p59	T	C	7: 107,703,256 (GRCm39)	S247P	probably damaging	Het
Or8c16	T	C	9: 38,130,706 (GRCm39)	Y193H	probably benign	Het
Ostm1	T	C	10: 42,574,210 (GRCm39)	V301A	probably damaging	Het
Pcdhb13	A	T	18: 37,577,608 (GRCm39)	D662V	probably damaging	Het
Pfpl	T	A	19: 12,406,374 (GRCm39)	S208R	probably damaging	Het
Plin5	A	T	17: 56,419,324 (GRCm39)	V366E	probably damaging	Het
Rmi1	A	G	13: 58,556,899 (GRCm39)	I383V	probably benign	Het
Rnh1	T	C	7: 140,740,714 (GRCm39)	T414A	probably benign	Het
Sash1	C	A	10: 8,617,296 (GRCm39)	G537W	probably damaging	Het
Slc9c1	A	G	16: 45,370,551 (GRCm39)	N264S	probably benign	Het
Speg	A	C	1: 75,398,420 (GRCm39)	T1956P	probably damaging	Het
St14	T	C	9: 31,002,905 (GRCm39)	Y666C	probably damaging	Het
Stab2	T	A	10: 86,727,481 (GRCm39)	H1448L	probably damaging	Het
Syne2	C	T	12: 76,154,230 (GRCm39)		probably benign	Het
Taf9	T	A	13: 100,790,860 (GRCm39)		probably benign	Het
Thumpd2	G	A	17: 81,334,040 (GRCm39)	A516V	possibly damaging	Het
Tmem132e	A	G	11: 82,335,308 (GRCm39)	K797E	probably damaging	Het
Tpte	A	G	8: 22,841,489 (GRCm39)	T494A	possibly damaging	Het
Ttc41	T	A	10: 86,567,113 (GRCm39)	L593Q	probably benign	Het
Uggt1	A	C	1: 36,249,103 (GRCm39)	I279S	possibly damaging	Het
Uxs1	A	G	1: 43,804,084 (GRCm39)	F277S	probably damaging	Het
Vat1	A	T	11: 101,351,380 (GRCm39)	N320K	possibly damaging	Het
Wdr81	G	T	11: 75,336,256 (GRCm39)	A592E		Het
Zfp780b	C	T	7: 27,673,143 (GRCm39)		probably null	Het
Zfp944	A	T	17: 22,562,619 (GRCm39)		probably null	Het
Zfp956	G	T	6: 47,934,437 (GRCm39)	G136W	possibly damaging	Het

Other mutations in Apob

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00402:Apob	APN	12	8,043,065 (GRCm39)	splice site	probably benign
IGL00421:Apob	APN	12	8,060,197 (GRCm39)	missense	probably damaging	0.99
IGL00658:Apob	APN	12	8,059,471 (GRCm39)	missense	probably benign	0.08
IGL00768:Apob	APN	12	8,052,107 (GRCm39)	missense	probably damaging	1.00
IGL00833:Apob	APN	12	8,060,101 (GRCm39)	missense	probably benign	0.14
IGL00926:Apob	APN	12	8,065,421 (GRCm39)	missense	probably benign	0.01
IGL01065:Apob	APN	12	8,053,299 (GRCm39)	missense	probably damaging	0.99
IGL01313:Apob	APN	12	8,050,898 (GRCm39)	missense	probably damaging	1.00
IGL01419:Apob	APN	12	8,052,251 (GRCm39)	missense	probably damaging	0.99
IGL01461:Apob	APN	12	8,051,884 (GRCm39)	missense	probably benign	0.13
IGL02002:Apob	APN	12	8,044,822 (GRCm39)	missense	probably benign	0.03
IGL02031:Apob	APN	12	8,065,222 (GRCm39)	missense	probably benign
IGL02102:Apob	APN	12	8,039,407 (GRCm39)	missense	possibly damaging	0.94
IGL02115:Apob	APN	12	8,042,923 (GRCm39)	missense	probably benign	0.06
IGL02513:Apob	APN	12	8,042,979 (GRCm39)	missense	probably benign	0.01
IGL02967:Apob	APN	12	8,065,366 (GRCm39)	nonsense	probably null
IGL03005:Apob	APN	12	8,043,059 (GRCm39)	splice site	probably benign
IGL03011:Apob	APN	12	8,047,883 (GRCm39)	missense	probably damaging	1.00
IGL03116:Apob	APN	12	8,066,350 (GRCm39)	missense	probably damaging	0.98
IGL03215:Apob	APN	12	8,063,818 (GRCm39)	missense	possibly damaging	0.92
IGL03227:Apob	APN	12	8,066,089 (GRCm39)	missense	probably benign	0.04
Aesthete	UTSW	12	8,060,080 (GRCm39)	nonsense	probably null
Essence	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
Ethos	UTSW	12	8,040,394 (GRCm39)	missense	probably null	1.00
IGL02835:Apob	UTSW	12	8,065,097 (GRCm39)	missense	possibly damaging	0.86
IGL02837:Apob	UTSW	12	8,055,102 (GRCm39)	missense	probably damaging	1.00
R0071:Apob	UTSW	12	8,052,111 (GRCm39)	missense	probably damaging	0.98
R0071:Apob	UTSW	12	8,052,111 (GRCm39)	missense	probably damaging	0.98
R0116:Apob	UTSW	12	8,039,113 (GRCm39)	unclassified	probably benign
R0180:Apob	UTSW	12	8,058,285 (GRCm39)	nonsense	probably null
R0288:Apob	UTSW	12	8,040,779 (GRCm39)	nonsense	probably null
R0295:Apob	UTSW	12	8,052,181 (GRCm39)	nonsense	probably null
R0305:Apob	UTSW	12	8,062,210 (GRCm39)	missense	probably damaging	1.00
R0312:Apob	UTSW	12	8,059,034 (GRCm39)	missense	probably benign
R0324:Apob	UTSW	12	8,060,521 (GRCm39)	missense	probably benign	0.41
R0326:Apob	UTSW	12	8,040,307 (GRCm39)	missense	probably damaging	1.00
R0363:Apob	UTSW	12	8,060,136 (GRCm39)	missense	probably damaging	1.00
R0390:Apob	UTSW	12	8,038,678 (GRCm39)	missense	probably damaging	0.99
R0462:Apob	UTSW	12	8,050,896 (GRCm39)	missense	probably damaging	1.00
R0471:Apob	UTSW	12	8,040,406 (GRCm39)	missense	probably damaging	1.00
R0532:Apob	UTSW	12	8,066,188 (GRCm39)	missense	possibly damaging	0.48
R0548:Apob	UTSW	12	8,056,282 (GRCm39)	missense	probably damaging	1.00
R0560:Apob	UTSW	12	8,055,101 (GRCm39)	missense	probably damaging	1.00
R0595:Apob	UTSW	12	8,058,369 (GRCm39)	missense	probably benign	0.01
R0600:Apob	UTSW	12	8,056,440 (GRCm39)	missense	probably damaging	1.00
R0626:Apob	UTSW	12	8,066,193 (GRCm39)	missense	probably benign	0.45
R0685:Apob	UTSW	12	8,060,742 (GRCm39)	missense	probably benign
R0765:Apob	UTSW	12	8,066,518 (GRCm39)	missense	probably benign
R0790:Apob	UTSW	12	8,060,245 (GRCm39)	missense	probably damaging	1.00
R0918:Apob	UTSW	12	8,033,941 (GRCm39)	missense	probably benign	0.10
R0962:Apob	UTSW	12	8,039,191 (GRCm39)	missense	probably damaging	0.98
R1055:Apob	UTSW	12	8,044,963 (GRCm39)	missense	probably damaging	1.00
R1077:Apob	UTSW	12	8,056,017 (GRCm39)	missense	probably benign
R1143:Apob	UTSW	12	8,062,354 (GRCm39)	missense	probably benign	0.26
R1163:Apob	UTSW	12	8,061,654 (GRCm39)	missense	probably damaging	1.00
R1266:Apob	UTSW	12	8,056,093 (GRCm39)	missense	probably benign	0.37
R1434:Apob	UTSW	12	8,059,715 (GRCm39)	missense	probably damaging	1.00
R1442:Apob	UTSW	12	8,036,165 (GRCm39)	missense	probably benign	0.31
R1445:Apob	UTSW	12	8,066,084 (GRCm39)	missense	possibly damaging	0.48
R1459:Apob	UTSW	12	8,061,937 (GRCm39)	missense	possibly damaging	0.92
R1459:Apob	UTSW	12	8,056,047 (GRCm39)	missense	probably benign
R1465:Apob	UTSW	12	8,061,421 (GRCm39)	missense	possibly damaging	0.91
R1465:Apob	UTSW	12	8,061,421 (GRCm39)	missense	possibly damaging	0.91
R1508:Apob	UTSW	12	8,061,481 (GRCm39)	missense	possibly damaging	0.92
R1518:Apob	UTSW	12	8,039,207 (GRCm39)	missense	probably benign	0.01
R1531:Apob	UTSW	12	8,047,880 (GRCm39)	missense	possibly damaging	0.65
R1547:Apob	UTSW	12	8,053,368 (GRCm39)	missense	probably benign	0.08
R1574:Apob	UTSW	12	8,040,839 (GRCm39)	missense	possibly damaging	0.51
R1574:Apob	UTSW	12	8,040,839 (GRCm39)	missense	possibly damaging	0.51
R1682:Apob	UTSW	12	8,062,365 (GRCm39)	missense	probably benign	0.00
R1709:Apob	UTSW	12	8,059,306 (GRCm39)	missense	probably damaging	0.98
R1718:Apob	UTSW	12	8,066,087 (GRCm39)	missense	probably benign	0.02
R1752:Apob	UTSW	12	8,038,766 (GRCm39)	missense	probably benign	0.01
R1781:Apob	UTSW	12	8,059,603 (GRCm39)	missense	possibly damaging	0.96
R1818:Apob	UTSW	12	8,063,064 (GRCm39)	missense	possibly damaging	0.93
R1818:Apob	UTSW	12	8,056,834 (GRCm39)	missense	probably damaging	0.98
R1842:Apob	UTSW	12	8,061,559 (GRCm39)	missense	probably damaging	1.00
R1843:Apob	UTSW	12	8,057,602 (GRCm39)	missense	possibly damaging	0.65
R1853:Apob	UTSW	12	8,060,928 (GRCm39)	nonsense	probably null
R1990:Apob	UTSW	12	8,051,039 (GRCm39)	missense	probably damaging	1.00
R2016:Apob	UTSW	12	8,057,751 (GRCm39)	missense	possibly damaging	0.48
R2017:Apob	UTSW	12	8,057,751 (GRCm39)	missense	possibly damaging	0.48
R2023:Apob	UTSW	12	8,061,090 (GRCm39)	missense	probably benign	0.01
R2037:Apob	UTSW	12	8,057,488 (GRCm39)	missense	probably benign	0.37
R2054:Apob	UTSW	12	8,063,134 (GRCm39)	missense	probably damaging	1.00
R2057:Apob	UTSW	12	8,052,164 (GRCm39)	nonsense	probably null
R2085:Apob	UTSW	12	8,062,240 (GRCm39)	missense	probably damaging	1.00
R2159:Apob	UTSW	12	8,060,081 (GRCm39)	missense	probably benign	0.12
R2209:Apob	UTSW	12	8,057,752 (GRCm39)	missense	probably benign	0.28
R2249:Apob	UTSW	12	8,057,499 (GRCm39)	missense	probably damaging	1.00
R2254:Apob	UTSW	12	8,061,256 (GRCm39)	missense	possibly damaging	0.92
R2265:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2266:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2267:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2268:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2296:Apob	UTSW	12	8,044,879 (GRCm39)	missense	probably damaging	0.97
R2897:Apob	UTSW	12	8,060,356 (GRCm39)	missense	probably damaging	1.00
R3431:Apob	UTSW	12	8,060,778 (GRCm39)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,056,327 (GRCm39)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,061,763 (GRCm39)	missense	possibly damaging	0.46
R3899:Apob	UTSW	12	8,065,849 (GRCm39)	missense	possibly damaging	0.87
R4020:Apob	UTSW	12	8,044,914 (GRCm39)	nonsense	probably null
R4050:Apob	UTSW	12	8,065,390 (GRCm39)	missense	probably benign	0.02
R4351:Apob	UTSW	12	8,043,054 (GRCm39)	missense	probably benign	0.03
R4365:Apob	UTSW	12	8,066,083 (GRCm39)	missense	possibly damaging	0.95
R4366:Apob	UTSW	12	8,066,083 (GRCm39)	missense	possibly damaging	0.95
R4456:Apob	UTSW	12	8,065,445 (GRCm39)	missense	probably damaging	1.00
R4458:Apob	UTSW	12	8,065,445 (GRCm39)	missense	probably damaging	1.00
R4600:Apob	UTSW	12	8,058,568 (GRCm39)	missense	probably damaging	1.00
R4611:Apob	UTSW	12	8,061,331 (GRCm39)	missense	probably damaging	1.00
R4646:Apob	UTSW	12	8,062,759 (GRCm39)	missense	probably benign	0.21
R4678:Apob	UTSW	12	8,045,585 (GRCm39)	missense	probably damaging	1.00
R4685:Apob	UTSW	12	8,056,456 (GRCm39)	missense	probably benign	0.00
R4707:Apob	UTSW	12	8,056,205 (GRCm39)	missense	probably damaging	0.96
R4726:Apob	UTSW	12	8,040,267 (GRCm39)	missense	probably damaging	0.98
R4792:Apob	UTSW	12	8,058,051 (GRCm39)	missense	probably benign	0.26
R4822:Apob	UTSW	12	8,065,741 (GRCm39)	missense	probably benign	0.04
R4834:Apob	UTSW	12	8,064,101 (GRCm39)	missense	possibly damaging	0.49
R4835:Apob	UTSW	12	8,065,391 (GRCm39)	missense	possibly damaging	0.56
R4887:Apob	UTSW	12	8,063,099 (GRCm39)	missense	probably damaging	1.00
R4910:Apob	UTSW	12	8,057,848 (GRCm39)	missense	probably damaging	1.00
R5072:Apob	UTSW	12	8,058,714 (GRCm39)	missense	probably benign	0.00
R5073:Apob	UTSW	12	8,055,219 (GRCm39)	critical splice donor site	probably null
R5074:Apob	UTSW	12	8,055,219 (GRCm39)	critical splice donor site	probably null
R5101:Apob	UTSW	12	8,061,934 (GRCm39)	missense	probably benign	0.09
R5123:Apob	UTSW	12	8,057,630 (GRCm39)	splice site	probably null
R5133:Apob	UTSW	12	8,058,898 (GRCm39)	missense	probably damaging	0.99
R5135:Apob	UTSW	12	8,060,086 (GRCm39)	missense	probably damaging	1.00
R5137:Apob	UTSW	12	8,061,384 (GRCm39)	missense	possibly damaging	0.63
R5160:Apob	UTSW	12	8,062,126 (GRCm39)	missense	possibly damaging	0.90
R5173:Apob	UTSW	12	8,058,238 (GRCm39)	missense	probably benign	0.00
R5202:Apob	UTSW	12	8,063,737 (GRCm39)	missense	probably damaging	0.98
R5229:Apob	UTSW	12	8,027,806 (GRCm39)	missense	probably benign
R5292:Apob	UTSW	12	8,055,912 (GRCm39)	missense	probably benign	0.01
R5378:Apob	UTSW	12	8,061,865 (GRCm39)	missense	probably damaging	0.99
R5494:Apob	UTSW	12	8,061,762 (GRCm39)	missense	probably damaging	0.99
R5517:Apob	UTSW	12	8,040,906 (GRCm39)	missense	probably damaging	1.00
R5576:Apob	UTSW	12	8,048,662 (GRCm39)	missense	probably damaging	1.00
R5582:Apob	UTSW	12	8,060,788 (GRCm39)	missense	probably damaging	1.00
R5629:Apob	UTSW	12	8,057,847 (GRCm39)	missense	probably damaging	1.00
R5678:Apob	UTSW	12	8,041,494 (GRCm39)	missense	possibly damaging	0.92
R5732:Apob	UTSW	12	8,060,353 (GRCm39)	missense	probably benign	0.15
R5734:Apob	UTSW	12	8,038,781 (GRCm39)	missense	probably damaging	1.00
R5742:Apob	UTSW	12	8,057,191 (GRCm39)	missense	probably damaging	1.00
R5751:Apob	UTSW	12	8,062,619 (GRCm39)	nonsense	probably null
R5776:Apob	UTSW	12	8,056,149 (GRCm39)	missense	possibly damaging	0.57
R5778:Apob	UTSW	12	8,065,074 (GRCm39)	missense	probably benign	0.45
R5783:Apob	UTSW	12	8,051,022 (GRCm39)	missense	probably damaging	1.00
R5786:Apob	UTSW	12	8,065,304 (GRCm39)	missense	possibly damaging	0.48
R5837:Apob	UTSW	12	8,053,277 (GRCm39)	missense	probably benign	0.04
R5857:Apob	UTSW	12	8,065,397 (GRCm39)	missense	probably benign	0.00
R6029:Apob	UTSW	12	8,066,243 (GRCm39)	missense	probably damaging	0.99
R6032:Apob	UTSW	12	8,045,513 (GRCm39)	missense	probably benign	0.02
R6032:Apob	UTSW	12	8,045,513 (GRCm39)	missense	probably benign	0.02
R6086:Apob	UTSW	12	8,065,164 (GRCm39)	missense	probably benign
R6110:Apob	UTSW	12	8,061,883 (GRCm39)	missense	probably damaging	1.00
R6131:Apob	UTSW	12	8,065,874 (GRCm39)	missense	probably benign	0.17
R6157:Apob	UTSW	12	8,056,077 (GRCm39)	missense	probably benign
R6179:Apob	UTSW	12	8,055,060 (GRCm39)	nonsense	probably null
R6247:Apob	UTSW	12	8,051,801 (GRCm39)	missense	probably damaging	1.00
R6279:Apob	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
R6300:Apob	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
R6320:Apob	UTSW	12	8,039,194 (GRCm39)	missense	probably benign	0.27
R6339:Apob	UTSW	12	8,066,188 (GRCm39)	missense	probably damaging	0.99
R6353:Apob	UTSW	12	8,059,421 (GRCm39)	missense	probably damaging	1.00
R6395:Apob	UTSW	12	8,058,507 (GRCm39)	missense	probably benign	0.45
R6441:Apob	UTSW	12	8,037,796 (GRCm39)	missense	probably damaging	1.00
R6492:Apob	UTSW	12	8,058,261 (GRCm39)	missense	probably damaging	0.99
R6495:Apob	UTSW	12	8,040,394 (GRCm39)	missense	probably null	1.00
R6502:Apob	UTSW	12	8,051,814 (GRCm39)	missense	probably damaging	0.99
R6520:Apob	UTSW	12	8,033,124 (GRCm39)	missense	probably damaging	1.00
R6644:Apob	UTSW	12	8,059,077 (GRCm39)	missense	probably damaging	0.97
R6704:Apob	UTSW	12	8,060,379 (GRCm39)	missense	probably damaging	0.98
R6750:Apob	UTSW	12	8,047,853 (GRCm39)	missense	probably damaging	1.00
R6759:Apob	UTSW	12	8,061,049 (GRCm39)	missense	probably benign	0.06
R6812:Apob	UTSW	12	8,033,062 (GRCm39)	missense	probably damaging	0.98
R6865:Apob	UTSW	12	8,058,847 (GRCm39)	missense	probably benign	0.05
R6873:Apob	UTSW	12	8,065,995 (GRCm39)	missense	probably benign	0.00
R7013:Apob	UTSW	12	8,060,080 (GRCm39)	nonsense	probably null
R7067:Apob	UTSW	12	8,059,423 (GRCm39)	missense	probably damaging	1.00
R7084:Apob	UTSW	12	8,059,591 (GRCm39)	missense	probably benign
R7113:Apob	UTSW	12	8,045,539 (GRCm39)	missense	probably damaging	1.00
R7175:Apob	UTSW	12	8,057,034 (GRCm39)	missense	probably benign	0.33
R7196:Apob	UTSW	12	8,033,893 (GRCm39)	missense	possibly damaging	0.90
R7199:Apob	UTSW	12	8,055,072 (GRCm39)	missense	probably damaging	1.00
R7205:Apob	UTSW	12	8,055,087 (GRCm39)	missense	probably damaging	0.98
R7251:Apob	UTSW	12	8,057,037 (GRCm39)	missense	probably damaging	0.98
R7474:Apob	UTSW	12	8,059,185 (GRCm39)	missense	probably benign	0.29
R7484:Apob	UTSW	12	8,056,884 (GRCm39)	nonsense	probably null
R7538:Apob	UTSW	12	8,052,219 (GRCm39)	missense	probably damaging	0.98
R7636:Apob	UTSW	12	8,059,516 (GRCm39)	missense	possibly damaging	0.86
R7646:Apob	UTSW	12	8,059,189 (GRCm39)	missense	probably damaging	0.99
R7787:Apob	UTSW	12	8,040,780 (GRCm39)	missense	probably damaging	0.97
R7793:Apob	UTSW	12	8,058,124 (GRCm39)	missense	probably damaging	0.99
R7836:Apob	UTSW	12	8,051,885 (GRCm39)	missense	possibly damaging	0.72
R7895:Apob	UTSW	12	8,061,933 (GRCm39)	missense	probably benign	0.00
R8005:Apob	UTSW	12	8,059,744 (GRCm39)	missense	probably benign	0.01
R8013:Apob	UTSW	12	8,060,798 (GRCm39)	missense	possibly damaging	0.94
R8014:Apob	UTSW	12	8,060,798 (GRCm39)	missense	possibly damaging	0.94
R8111:Apob	UTSW	12	8,058,801 (GRCm39)	missense	probably benign	0.16
R8117:Apob	UTSW	12	8,056,435 (GRCm39)	missense	probably damaging	0.99
R8226:Apob	UTSW	12	8,059,056 (GRCm39)	missense	probably benign	0.00
R8244:Apob	UTSW	12	8,060,548 (GRCm39)	missense	probably damaging	0.96
R8280:Apob	UTSW	12	8,060,851 (GRCm39)	missense	possibly damaging	0.46
R8310:Apob	UTSW	12	8,059,033 (GRCm39)	missense	probably benign	0.00
R8327:Apob	UTSW	12	8,051,015 (GRCm39)	missense	possibly damaging	0.72
R8329:Apob	UTSW	12	8,061,135 (GRCm39)	missense	probably damaging	0.98
R8331:Apob	UTSW	12	8,051,882 (GRCm39)	missense	probably benign	0.28
R8351:Apob	UTSW	12	8,056,356 (GRCm39)	missense	probably benign	0.29
R8412:Apob	UTSW	12	8,058,069 (GRCm39)	missense	probably benign	0.33
R8425:Apob	UTSW	12	8,038,842 (GRCm39)	missense	possibly damaging	0.70
R8481:Apob	UTSW	12	8,044,807 (GRCm39)	splice site	probably null
R8493:Apob	UTSW	12	8,059,009 (GRCm39)	missense	possibly damaging	0.87
R8529:Apob	UTSW	12	8,057,353 (GRCm39)	missense	probably damaging	1.00
R8554:Apob	UTSW	12	8,037,830 (GRCm39)	missense	probably damaging	0.98
R8692:Apob	UTSW	12	8,058,270 (GRCm39)	missense	probably damaging	0.98
R8695:Apob	UTSW	12	8,057,830 (GRCm39)	missense	probably damaging	1.00
R8977:Apob	UTSW	12	8,065,990 (GRCm39)	missense	probably damaging	0.99
R9016:Apob	UTSW	12	8,035,408 (GRCm39)	splice site	silent
R9020:Apob	UTSW	12	8,063,999 (GRCm39)	missense	probably damaging	1.00
R9037:Apob	UTSW	12	8,066,501 (GRCm39)	missense	probably benign	0.15
R9053:Apob	UTSW	12	8,058,954 (GRCm39)	missense	possibly damaging	0.72
R9062:Apob	UTSW	12	8,058,046 (GRCm39)	missense	possibly damaging	0.91
R9142:Apob	UTSW	12	8,062,705 (GRCm39)	missense	possibly damaging	0.95
R9180:Apob	UTSW	12	8,047,925 (GRCm39)	missense	probably damaging	1.00
R9205:Apob	UTSW	12	8,030,635 (GRCm39)	missense	probably damaging	0.99
R9277:Apob	UTSW	12	8,061,183 (GRCm39)	missense	probably benign	0.01
R9305:Apob	UTSW	12	8,058,053 (GRCm39)	missense	probably benign	0.04
R9358:Apob	UTSW	12	8,060,833 (GRCm39)	missense	probably benign	0.14
R9375:Apob	UTSW	12	8,029,261 (GRCm39)	missense	possibly damaging	0.91
R9385:Apob	UTSW	12	8,056,399 (GRCm39)	missense	possibly damaging	0.91
R9386:Apob	UTSW	12	8,056,629 (GRCm39)	missense	probably damaging	0.99
R9392:Apob	UTSW	12	8,057,098 (GRCm39)	missense	probably benign	0.45
R9470:Apob	UTSW	12	8,039,219 (GRCm39)	missense	possibly damaging	0.94
R9523:Apob	UTSW	12	8,052,069 (GRCm39)	missense	probably damaging	1.00
R9545:Apob	UTSW	12	8,033,890 (GRCm39)	missense	possibly damaging	0.81
R9629:Apob	UTSW	12	8,059,054 (GRCm39)	missense	probably damaging	1.00
R9702:Apob	UTSW	12	8,057,559 (GRCm39)	missense	probably damaging	0.96
R9703:Apob	UTSW	12	8,030,507 (GRCm39)	missense	probably damaging	0.99
R9719:Apob	UTSW	12	8,065,464 (GRCm39)	missense	probably benign	0.15
R9726:Apob	UTSW	12	8,056,926 (GRCm39)	missense	probably damaging	0.99
R9729:Apob	UTSW	12	8,066,125 (GRCm39)	missense	probably damaging	0.99
X0027:Apob	UTSW	12	8,057,975 (GRCm39)	missense	probably benign
Z1088:Apob	UTSW	12	8,062,936 (GRCm39)	missense	possibly damaging	0.95
Z1088:Apob	UTSW	12	8,055,945 (GRCm39)	nonsense	probably null
Z1088:Apob	UTSW	12	8,055,074 (GRCm39)	missense	possibly damaging	0.91
Z1176:Apob	UTSW	12	8,054,978 (GRCm39)	missense	probably benign	0.00
Z1176:Apob	UTSW	12	8,048,011 (GRCm39)	missense	probably damaging	1.00
Z1177:Apob	UTSW	12	8,065,249 (GRCm39)	frame shift	probably null
Z1177:Apob	UTSW	12	8,038,765 (GRCm39)	missense	probably benign	0.43

Predicted Primers

PCR Primer
(F):5'- CAGCATATTCAAAACTGAGTGGAG -3'
(R):5'- AGTCCATCAGCCACATGACTG -3'

Sequencing Primer
(F):5'- TATTCAAAACTGAGTGGAGGTACAAG -3'
(R):5'- ACTGCCATGTGGTACTTCTGAGTAAC -3'

Posted On

2022-03-25