Mutagenetix > Incidental Mutation

Incidental Mutation 'R7636:Apob'

589973

Institutional Source

Beutler Lab

Gene Symbol

Apob

Ensembl Gene

ENSMUSG00000020609

Gene Name

apolipoprotein B

Synonyms

apob-100, apob-48

MMRRC Submission

045722-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.859) question?

Stock #

R7636 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

8027648-8066835 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 8059516 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 2666 (I2666T)

Ref Sequence

ENSEMBL: ENSMUSP00000036044 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037520] [ENSMUST00000037811] [ENSMUST00000171239]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000037520
AA Change: I2633T

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000035761
Gene: ENSMUSG00000020609
AA Change: I2633T

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	33	585	6.03e-94	SMART
DUF1943	619	932	7.88e-97	SMART
Pfam:DUF1081	945	1059	9.4e-32	PFAM
low complexity region	1100	1109	N/A	INTRINSIC
Blast:LPD_N	1249	1311	9e-22	BLAST
low complexity region	1632	1644	N/A	INTRINSIC
internal_repeat_1	1882	2038	6.61e-9	PROSPERO
SCOP:d1gw5a_	2105	2577	9e-5	SMART
internal_repeat_1	2973	3150	6.61e-9	PROSPERO
low complexity region	3561	3580	N/A	INTRINSIC
low complexity region	3928	3936	N/A	INTRINSIC
Pfam:ApoB100_C	4401	4456	5.6e-34	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000037811
AA Change: I2666T

PolyPhen 2 Score 0.861 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000036044
Gene: ENSMUSG00000020609
AA Change: I2666T

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	46	598	6.03e-94	SMART
DUF1943	632	945	7.88e-97	SMART
Pfam:DUF1081	960	1070	6.3e-39	PFAM
low complexity region	1113	1122	N/A	INTRINSIC
Blast:LPD_N	1282	1344	1e-21	BLAST
low complexity region	1665	1677	N/A	INTRINSIC
internal_repeat_1	1915	2071	6.6e-9	PROSPERO
SCOP:d1gw5a_	2138	2610	9e-5	SMART
internal_repeat_1	3006	3183	6.6e-9	PROSPERO
low complexity region	3594	3613	N/A	INTRINSIC
low complexity region	3961	3969	N/A	INTRINSIC
Pfam:ApoB100_C	4434	4490	1.6e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171239

SMART Domains

Protein: ENSMUSP00000129496
Gene: ENSMUSG00000020609

Domain	Start	End	E-Value	Type
low complexity region	348	356	N/A	INTRINSIC

Meta Mutation Damage Score

0.1376

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (55/55)

MGI Phenotype

FUNCTION: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mutants usually die by midgestation and longer survivors exhibit exencephaly. Heterozygotes show reduced plasma cholesterol and apolipoprotein levels. Single isoform B100 and B48 null mutants are viable. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610318N02Rik	C	T	16: 16,935,701 (GRCm39)	E199K	possibly damaging	Het
Akap13	C	A	7: 75,259,621 (GRCm39)	N748K	probably benign	Het
Aldh16a1	G	T	7: 44,796,955 (GRCm39)	P58T	unknown	Het
Amhr2	T	A	15: 102,360,893 (GRCm39)	C343S	probably damaging	Het
Ankrd17	A	T	5: 90,380,239 (GRCm39)	M2483K	possibly damaging	Het
Ano3	A	T	2: 110,513,048 (GRCm39)	L649*	probably null	Het
C1ql3	A	T	2: 13,015,621 (GRCm39)	V13E	probably benign	Het
Casp12	A	G	9: 5,358,344 (GRCm39)	I384V	probably benign	Het
Cimip2a	A	T	2: 25,108,832 (GRCm39)	H7L	probably damaging	Het
Clcn1	A	T	6: 42,268,268 (GRCm39)	K147*	probably null	Het
Ctnna1	T	A	18: 35,356,526 (GRCm39)	N398K	possibly damaging	Het
Dcdc2a	A	T	13: 25,286,605 (GRCm39)	K176M	probably damaging	Het
Depdc5	T	A	5: 33,075,327 (GRCm39)	S502T	probably benign	Het
Fam83e	G	A	7: 45,376,450 (GRCm39)	D388N	probably damaging	Het
Fbxl15	G	A	19: 46,317,614 (GRCm39)	E99K	probably benign	Het
Fbxw2	T	C	2: 34,702,956 (GRCm39)	T186A	probably benign	Het
Frem2	T	A	3: 53,560,668 (GRCm39)	M1280L	probably benign	Het
Gbgt1	T	A	2: 28,395,326 (GRCm39)	D321E	probably damaging	Het
Gsdmc2	T	A	15: 63,700,041 (GRCm39)	T244S	probably benign	Het
Heatr5a	C	A	12: 51,934,979 (GRCm39)	G1675W	probably damaging	Het
Heatr5a	G	A	12: 51,999,341 (GRCm39)	L287F	probably damaging	Het
Hfm1	C	A	5: 107,065,332 (GRCm39)	A116S	probably benign	Het
Insr	A	G	8: 3,308,709 (GRCm39)	I109T	probably damaging	Het
Itga4	A	G	2: 79,144,176 (GRCm39)	I688V	probably benign	Het
Lipe	A	G	7: 25,088,042 (GRCm39)	M49T	probably benign	Het
Lrriq3	A	G	3: 154,893,787 (GRCm39)	Y496C	probably damaging	Het
Lyst	T	C	13: 13,791,332 (GRCm39)		probably null	Het
Megf10	T	A	18: 57,410,061 (GRCm39)	I614N	possibly damaging	Het
Mkx	A	G	18: 7,000,630 (GRCm39)	I104T	possibly damaging	Het
Mrgpra4	C	A	7: 47,630,721 (GRCm39)	L293F	possibly damaging	Het
Olfm2	A	G	9: 20,579,593 (GRCm39)	V386A	possibly damaging	Het
Or2w3b	A	T	11: 58,623,273 (GRCm39)	N239K	possibly damaging	Het
Or4a15	A	T	2: 89,193,583 (GRCm39)	Y63*	probably null	Het
Or52s6	C	A	7: 103,092,040 (GRCm39)	D97Y	probably damaging	Het
Pkd2l1	G	T	19: 44,179,870 (GRCm39)	D113E	possibly damaging	Het
Rab11fip2	A	T	19: 59,931,317 (GRCm39)	I39K	possibly damaging	Het
Rab1b	A	T	19: 5,150,810 (GRCm39)	V99E	probably damaging	Het
Rad54l2	A	T	9: 106,597,586 (GRCm39)	L140Q	probably damaging	Het
Repin1	T	C	6: 48,573,299 (GRCm39)	F76S	probably benign	Het
Robo1	A	G	16: 72,360,615 (GRCm39)	D9G		Het
Scn3a	A	G	2: 65,328,033 (GRCm39)	L819P	probably damaging	Het
Scn7a	A	T	2: 66,574,172 (GRCm39)	N150K	possibly damaging	Het
Sh2d3c	T	C	2: 32,615,023 (GRCm39)	S38P	probably benign	Het
Sin3b	A	T	8: 73,474,362 (GRCm39)	K549*	probably null	Het
Sorl1	A	G	9: 42,003,630 (GRCm39)	F222S	possibly damaging	Het
Spata31h1	T	A	10: 82,130,973 (GRCm39)	D679V	probably benign	Het
Tmem147	A	T	7: 30,427,726 (GRCm39)		probably null	Het
Tmem161b	C	A	13: 84,370,537 (GRCm39)		probably benign	Het
Tmem179	C	T	12: 112,477,177 (GRCm39)		probably null	Het
Tubg2	A	T	11: 101,050,913 (GRCm39)	I213F	probably damaging	Het
Zfhx3	T	C	8: 109,673,441 (GRCm39)	I1497T	probably benign	Het
Zfp128	A	T	7: 12,624,039 (GRCm39)	M136L	probably benign	Het
Zzz3	G	A	3: 152,133,289 (GRCm39)	A116T	probably benign	Het

Other mutations in Apob

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00402:Apob	APN	12	8,043,065 (GRCm39)	splice site	probably benign
IGL00421:Apob	APN	12	8,060,197 (GRCm39)	missense	probably damaging	0.99
IGL00658:Apob	APN	12	8,059,471 (GRCm39)	missense	probably benign	0.08
IGL00768:Apob	APN	12	8,052,107 (GRCm39)	missense	probably damaging	1.00
IGL00833:Apob	APN	12	8,060,101 (GRCm39)	missense	probably benign	0.14
IGL00926:Apob	APN	12	8,065,421 (GRCm39)	missense	probably benign	0.01
IGL01065:Apob	APN	12	8,053,299 (GRCm39)	missense	probably damaging	0.99
IGL01313:Apob	APN	12	8,050,898 (GRCm39)	missense	probably damaging	1.00
IGL01419:Apob	APN	12	8,052,251 (GRCm39)	missense	probably damaging	0.99
IGL01461:Apob	APN	12	8,051,884 (GRCm39)	missense	probably benign	0.13
IGL02002:Apob	APN	12	8,044,822 (GRCm39)	missense	probably benign	0.03
IGL02031:Apob	APN	12	8,065,222 (GRCm39)	missense	probably benign
IGL02102:Apob	APN	12	8,039,407 (GRCm39)	missense	possibly damaging	0.94
IGL02115:Apob	APN	12	8,042,923 (GRCm39)	missense	probably benign	0.06
IGL02513:Apob	APN	12	8,042,979 (GRCm39)	missense	probably benign	0.01
IGL02967:Apob	APN	12	8,065,366 (GRCm39)	nonsense	probably null
IGL03005:Apob	APN	12	8,043,059 (GRCm39)	splice site	probably benign
IGL03011:Apob	APN	12	8,047,883 (GRCm39)	missense	probably damaging	1.00
IGL03116:Apob	APN	12	8,066,350 (GRCm39)	missense	probably damaging	0.98
IGL03215:Apob	APN	12	8,063,818 (GRCm39)	missense	possibly damaging	0.92
IGL03227:Apob	APN	12	8,066,089 (GRCm39)	missense	probably benign	0.04
Aesthete	UTSW	12	8,060,080 (GRCm39)	nonsense	probably null
Essence	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
Ethos	UTSW	12	8,040,394 (GRCm39)	missense	probably null	1.00
IGL02835:Apob	UTSW	12	8,065,097 (GRCm39)	missense	possibly damaging	0.86
IGL02837:Apob	UTSW	12	8,055,102 (GRCm39)	missense	probably damaging	1.00
R0071:Apob	UTSW	12	8,052,111 (GRCm39)	missense	probably damaging	0.98
R0071:Apob	UTSW	12	8,052,111 (GRCm39)	missense	probably damaging	0.98
R0116:Apob	UTSW	12	8,039,113 (GRCm39)	unclassified	probably benign
R0180:Apob	UTSW	12	8,058,285 (GRCm39)	nonsense	probably null
R0288:Apob	UTSW	12	8,040,779 (GRCm39)	nonsense	probably null
R0295:Apob	UTSW	12	8,052,181 (GRCm39)	nonsense	probably null
R0305:Apob	UTSW	12	8,062,210 (GRCm39)	missense	probably damaging	1.00
R0312:Apob	UTSW	12	8,059,034 (GRCm39)	missense	probably benign
R0324:Apob	UTSW	12	8,060,521 (GRCm39)	missense	probably benign	0.41
R0326:Apob	UTSW	12	8,040,307 (GRCm39)	missense	probably damaging	1.00
R0363:Apob	UTSW	12	8,060,136 (GRCm39)	missense	probably damaging	1.00
R0390:Apob	UTSW	12	8,038,678 (GRCm39)	missense	probably damaging	0.99
R0462:Apob	UTSW	12	8,050,896 (GRCm39)	missense	probably damaging	1.00
R0471:Apob	UTSW	12	8,040,406 (GRCm39)	missense	probably damaging	1.00
R0532:Apob	UTSW	12	8,066,188 (GRCm39)	missense	possibly damaging	0.48
R0548:Apob	UTSW	12	8,056,282 (GRCm39)	missense	probably damaging	1.00
R0560:Apob	UTSW	12	8,055,101 (GRCm39)	missense	probably damaging	1.00
R0595:Apob	UTSW	12	8,058,369 (GRCm39)	missense	probably benign	0.01
R0600:Apob	UTSW	12	8,056,440 (GRCm39)	missense	probably damaging	1.00
R0626:Apob	UTSW	12	8,066,193 (GRCm39)	missense	probably benign	0.45
R0685:Apob	UTSW	12	8,060,742 (GRCm39)	missense	probably benign
R0765:Apob	UTSW	12	8,066,518 (GRCm39)	missense	probably benign
R0790:Apob	UTSW	12	8,060,245 (GRCm39)	missense	probably damaging	1.00
R0918:Apob	UTSW	12	8,033,941 (GRCm39)	missense	probably benign	0.10
R0962:Apob	UTSW	12	8,039,191 (GRCm39)	missense	probably damaging	0.98
R1055:Apob	UTSW	12	8,044,963 (GRCm39)	missense	probably damaging	1.00
R1077:Apob	UTSW	12	8,056,017 (GRCm39)	missense	probably benign
R1143:Apob	UTSW	12	8,062,354 (GRCm39)	missense	probably benign	0.26
R1163:Apob	UTSW	12	8,061,654 (GRCm39)	missense	probably damaging	1.00
R1266:Apob	UTSW	12	8,056,093 (GRCm39)	missense	probably benign	0.37
R1434:Apob	UTSW	12	8,059,715 (GRCm39)	missense	probably damaging	1.00
R1442:Apob	UTSW	12	8,036,165 (GRCm39)	missense	probably benign	0.31
R1445:Apob	UTSW	12	8,066,084 (GRCm39)	missense	possibly damaging	0.48
R1459:Apob	UTSW	12	8,061,937 (GRCm39)	missense	possibly damaging	0.92
R1459:Apob	UTSW	12	8,056,047 (GRCm39)	missense	probably benign
R1465:Apob	UTSW	12	8,061,421 (GRCm39)	missense	possibly damaging	0.91
R1465:Apob	UTSW	12	8,061,421 (GRCm39)	missense	possibly damaging	0.91
R1508:Apob	UTSW	12	8,061,481 (GRCm39)	missense	possibly damaging	0.92
R1518:Apob	UTSW	12	8,039,207 (GRCm39)	missense	probably benign	0.01
R1531:Apob	UTSW	12	8,047,880 (GRCm39)	missense	possibly damaging	0.65
R1547:Apob	UTSW	12	8,053,368 (GRCm39)	missense	probably benign	0.08
R1574:Apob	UTSW	12	8,040,839 (GRCm39)	missense	possibly damaging	0.51
R1574:Apob	UTSW	12	8,040,839 (GRCm39)	missense	possibly damaging	0.51
R1682:Apob	UTSW	12	8,062,365 (GRCm39)	missense	probably benign	0.00
R1709:Apob	UTSW	12	8,059,306 (GRCm39)	missense	probably damaging	0.98
R1718:Apob	UTSW	12	8,066,087 (GRCm39)	missense	probably benign	0.02
R1752:Apob	UTSW	12	8,038,766 (GRCm39)	missense	probably benign	0.01
R1781:Apob	UTSW	12	8,059,603 (GRCm39)	missense	possibly damaging	0.96
R1818:Apob	UTSW	12	8,063,064 (GRCm39)	missense	possibly damaging	0.93
R1818:Apob	UTSW	12	8,056,834 (GRCm39)	missense	probably damaging	0.98
R1842:Apob	UTSW	12	8,061,559 (GRCm39)	missense	probably damaging	1.00
R1843:Apob	UTSW	12	8,057,602 (GRCm39)	missense	possibly damaging	0.65
R1853:Apob	UTSW	12	8,060,928 (GRCm39)	nonsense	probably null
R1990:Apob	UTSW	12	8,051,039 (GRCm39)	missense	probably damaging	1.00
R2016:Apob	UTSW	12	8,057,751 (GRCm39)	missense	possibly damaging	0.48
R2017:Apob	UTSW	12	8,057,751 (GRCm39)	missense	possibly damaging	0.48
R2023:Apob	UTSW	12	8,061,090 (GRCm39)	missense	probably benign	0.01
R2037:Apob	UTSW	12	8,057,488 (GRCm39)	missense	probably benign	0.37
R2054:Apob	UTSW	12	8,063,134 (GRCm39)	missense	probably damaging	1.00
R2057:Apob	UTSW	12	8,052,164 (GRCm39)	nonsense	probably null
R2085:Apob	UTSW	12	8,062,240 (GRCm39)	missense	probably damaging	1.00
R2159:Apob	UTSW	12	8,060,081 (GRCm39)	missense	probably benign	0.12
R2209:Apob	UTSW	12	8,057,752 (GRCm39)	missense	probably benign	0.28
R2249:Apob	UTSW	12	8,057,499 (GRCm39)	missense	probably damaging	1.00
R2254:Apob	UTSW	12	8,061,256 (GRCm39)	missense	possibly damaging	0.92
R2265:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2266:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2267:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2268:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2296:Apob	UTSW	12	8,044,879 (GRCm39)	missense	probably damaging	0.97
R2897:Apob	UTSW	12	8,060,356 (GRCm39)	missense	probably damaging	1.00
R3431:Apob	UTSW	12	8,060,778 (GRCm39)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,056,327 (GRCm39)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,061,763 (GRCm39)	missense	possibly damaging	0.46
R3899:Apob	UTSW	12	8,065,849 (GRCm39)	missense	possibly damaging	0.87
R4020:Apob	UTSW	12	8,044,914 (GRCm39)	nonsense	probably null
R4050:Apob	UTSW	12	8,065,390 (GRCm39)	missense	probably benign	0.02
R4351:Apob	UTSW	12	8,043,054 (GRCm39)	missense	probably benign	0.03
R4365:Apob	UTSW	12	8,066,083 (GRCm39)	missense	possibly damaging	0.95
R4366:Apob	UTSW	12	8,066,083 (GRCm39)	missense	possibly damaging	0.95
R4456:Apob	UTSW	12	8,065,445 (GRCm39)	missense	probably damaging	1.00
R4458:Apob	UTSW	12	8,065,445 (GRCm39)	missense	probably damaging	1.00
R4600:Apob	UTSW	12	8,058,568 (GRCm39)	missense	probably damaging	1.00
R4611:Apob	UTSW	12	8,061,331 (GRCm39)	missense	probably damaging	1.00
R4646:Apob	UTSW	12	8,062,759 (GRCm39)	missense	probably benign	0.21
R4678:Apob	UTSW	12	8,045,585 (GRCm39)	missense	probably damaging	1.00
R4685:Apob	UTSW	12	8,056,456 (GRCm39)	missense	probably benign	0.00
R4707:Apob	UTSW	12	8,056,205 (GRCm39)	missense	probably damaging	0.96
R4726:Apob	UTSW	12	8,040,267 (GRCm39)	missense	probably damaging	0.98
R4792:Apob	UTSW	12	8,058,051 (GRCm39)	missense	probably benign	0.26
R4822:Apob	UTSW	12	8,065,741 (GRCm39)	missense	probably benign	0.04
R4834:Apob	UTSW	12	8,064,101 (GRCm39)	missense	possibly damaging	0.49
R4835:Apob	UTSW	12	8,065,391 (GRCm39)	missense	possibly damaging	0.56
R4887:Apob	UTSW	12	8,063,099 (GRCm39)	missense	probably damaging	1.00
R4910:Apob	UTSW	12	8,057,848 (GRCm39)	missense	probably damaging	1.00
R5072:Apob	UTSW	12	8,058,714 (GRCm39)	missense	probably benign	0.00
R5073:Apob	UTSW	12	8,055,219 (GRCm39)	critical splice donor site	probably null
R5074:Apob	UTSW	12	8,055,219 (GRCm39)	critical splice donor site	probably null
R5101:Apob	UTSW	12	8,061,934 (GRCm39)	missense	probably benign	0.09
R5123:Apob	UTSW	12	8,057,630 (GRCm39)	splice site	probably null
R5133:Apob	UTSW	12	8,058,898 (GRCm39)	missense	probably damaging	0.99
R5135:Apob	UTSW	12	8,060,086 (GRCm39)	missense	probably damaging	1.00
R5137:Apob	UTSW	12	8,061,384 (GRCm39)	missense	possibly damaging	0.63
R5160:Apob	UTSW	12	8,062,126 (GRCm39)	missense	possibly damaging	0.90
R5173:Apob	UTSW	12	8,058,238 (GRCm39)	missense	probably benign	0.00
R5202:Apob	UTSW	12	8,063,737 (GRCm39)	missense	probably damaging	0.98
R5229:Apob	UTSW	12	8,027,806 (GRCm39)	missense	probably benign
R5292:Apob	UTSW	12	8,055,912 (GRCm39)	missense	probably benign	0.01
R5378:Apob	UTSW	12	8,061,865 (GRCm39)	missense	probably damaging	0.99
R5494:Apob	UTSW	12	8,061,762 (GRCm39)	missense	probably damaging	0.99
R5517:Apob	UTSW	12	8,040,906 (GRCm39)	missense	probably damaging	1.00
R5576:Apob	UTSW	12	8,048,662 (GRCm39)	missense	probably damaging	1.00
R5582:Apob	UTSW	12	8,060,788 (GRCm39)	missense	probably damaging	1.00
R5629:Apob	UTSW	12	8,057,847 (GRCm39)	missense	probably damaging	1.00
R5678:Apob	UTSW	12	8,041,494 (GRCm39)	missense	possibly damaging	0.92
R5732:Apob	UTSW	12	8,060,353 (GRCm39)	missense	probably benign	0.15
R5734:Apob	UTSW	12	8,038,781 (GRCm39)	missense	probably damaging	1.00
R5742:Apob	UTSW	12	8,057,191 (GRCm39)	missense	probably damaging	1.00
R5751:Apob	UTSW	12	8,062,619 (GRCm39)	nonsense	probably null
R5776:Apob	UTSW	12	8,056,149 (GRCm39)	missense	possibly damaging	0.57
R5778:Apob	UTSW	12	8,065,074 (GRCm39)	missense	probably benign	0.45
R5783:Apob	UTSW	12	8,051,022 (GRCm39)	missense	probably damaging	1.00
R5786:Apob	UTSW	12	8,065,304 (GRCm39)	missense	possibly damaging	0.48
R5837:Apob	UTSW	12	8,053,277 (GRCm39)	missense	probably benign	0.04
R5857:Apob	UTSW	12	8,065,397 (GRCm39)	missense	probably benign	0.00
R6029:Apob	UTSW	12	8,066,243 (GRCm39)	missense	probably damaging	0.99
R6032:Apob	UTSW	12	8,045,513 (GRCm39)	missense	probably benign	0.02
R6032:Apob	UTSW	12	8,045,513 (GRCm39)	missense	probably benign	0.02
R6086:Apob	UTSW	12	8,065,164 (GRCm39)	missense	probably benign
R6110:Apob	UTSW	12	8,061,883 (GRCm39)	missense	probably damaging	1.00
R6131:Apob	UTSW	12	8,065,874 (GRCm39)	missense	probably benign	0.17
R6157:Apob	UTSW	12	8,056,077 (GRCm39)	missense	probably benign
R6179:Apob	UTSW	12	8,055,060 (GRCm39)	nonsense	probably null
R6247:Apob	UTSW	12	8,051,801 (GRCm39)	missense	probably damaging	1.00
R6279:Apob	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
R6300:Apob	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
R6320:Apob	UTSW	12	8,039,194 (GRCm39)	missense	probably benign	0.27
R6339:Apob	UTSW	12	8,066,188 (GRCm39)	missense	probably damaging	0.99
R6353:Apob	UTSW	12	8,059,421 (GRCm39)	missense	probably damaging	1.00
R6395:Apob	UTSW	12	8,058,507 (GRCm39)	missense	probably benign	0.45
R6441:Apob	UTSW	12	8,037,796 (GRCm39)	missense	probably damaging	1.00
R6492:Apob	UTSW	12	8,058,261 (GRCm39)	missense	probably damaging	0.99
R6495:Apob	UTSW	12	8,040,394 (GRCm39)	missense	probably null	1.00
R6502:Apob	UTSW	12	8,051,814 (GRCm39)	missense	probably damaging	0.99
R6520:Apob	UTSW	12	8,033,124 (GRCm39)	missense	probably damaging	1.00
R6644:Apob	UTSW	12	8,059,077 (GRCm39)	missense	probably damaging	0.97
R6704:Apob	UTSW	12	8,060,379 (GRCm39)	missense	probably damaging	0.98
R6750:Apob	UTSW	12	8,047,853 (GRCm39)	missense	probably damaging	1.00
R6759:Apob	UTSW	12	8,061,049 (GRCm39)	missense	probably benign	0.06
R6812:Apob	UTSW	12	8,033,062 (GRCm39)	missense	probably damaging	0.98
R6865:Apob	UTSW	12	8,058,847 (GRCm39)	missense	probably benign	0.05
R6873:Apob	UTSW	12	8,065,995 (GRCm39)	missense	probably benign	0.00
R7013:Apob	UTSW	12	8,060,080 (GRCm39)	nonsense	probably null
R7067:Apob	UTSW	12	8,059,423 (GRCm39)	missense	probably damaging	1.00
R7084:Apob	UTSW	12	8,059,591 (GRCm39)	missense	probably benign
R7113:Apob	UTSW	12	8,045,539 (GRCm39)	missense	probably damaging	1.00
R7175:Apob	UTSW	12	8,057,034 (GRCm39)	missense	probably benign	0.33
R7196:Apob	UTSW	12	8,033,893 (GRCm39)	missense	possibly damaging	0.90
R7199:Apob	UTSW	12	8,055,072 (GRCm39)	missense	probably damaging	1.00
R7205:Apob	UTSW	12	8,055,087 (GRCm39)	missense	probably damaging	0.98
R7251:Apob	UTSW	12	8,057,037 (GRCm39)	missense	probably damaging	0.98
R7474:Apob	UTSW	12	8,059,185 (GRCm39)	missense	probably benign	0.29
R7484:Apob	UTSW	12	8,056,884 (GRCm39)	nonsense	probably null
R7538:Apob	UTSW	12	8,052,219 (GRCm39)	missense	probably damaging	0.98
R7646:Apob	UTSW	12	8,059,189 (GRCm39)	missense	probably damaging	0.99
R7787:Apob	UTSW	12	8,040,780 (GRCm39)	missense	probably damaging	0.97
R7793:Apob	UTSW	12	8,058,124 (GRCm39)	missense	probably damaging	0.99
R7836:Apob	UTSW	12	8,051,885 (GRCm39)	missense	possibly damaging	0.72
R7895:Apob	UTSW	12	8,061,933 (GRCm39)	missense	probably benign	0.00
R8005:Apob	UTSW	12	8,059,744 (GRCm39)	missense	probably benign	0.01
R8013:Apob	UTSW	12	8,060,798 (GRCm39)	missense	possibly damaging	0.94
R8014:Apob	UTSW	12	8,060,798 (GRCm39)	missense	possibly damaging	0.94
R8111:Apob	UTSW	12	8,058,801 (GRCm39)	missense	probably benign	0.16
R8117:Apob	UTSW	12	8,056,435 (GRCm39)	missense	probably damaging	0.99
R8226:Apob	UTSW	12	8,059,056 (GRCm39)	missense	probably benign	0.00
R8244:Apob	UTSW	12	8,060,548 (GRCm39)	missense	probably damaging	0.96
R8280:Apob	UTSW	12	8,060,851 (GRCm39)	missense	possibly damaging	0.46
R8310:Apob	UTSW	12	8,059,033 (GRCm39)	missense	probably benign	0.00
R8327:Apob	UTSW	12	8,051,015 (GRCm39)	missense	possibly damaging	0.72
R8329:Apob	UTSW	12	8,061,135 (GRCm39)	missense	probably damaging	0.98
R8331:Apob	UTSW	12	8,051,882 (GRCm39)	missense	probably benign	0.28
R8351:Apob	UTSW	12	8,056,356 (GRCm39)	missense	probably benign	0.29
R8412:Apob	UTSW	12	8,058,069 (GRCm39)	missense	probably benign	0.33
R8425:Apob	UTSW	12	8,038,842 (GRCm39)	missense	possibly damaging	0.70
R8481:Apob	UTSW	12	8,044,807 (GRCm39)	splice site	probably null
R8493:Apob	UTSW	12	8,059,009 (GRCm39)	missense	possibly damaging	0.87
R8529:Apob	UTSW	12	8,057,353 (GRCm39)	missense	probably damaging	1.00
R8554:Apob	UTSW	12	8,037,830 (GRCm39)	missense	probably damaging	0.98
R8692:Apob	UTSW	12	8,058,270 (GRCm39)	missense	probably damaging	0.98
R8695:Apob	UTSW	12	8,057,830 (GRCm39)	missense	probably damaging	1.00
R8977:Apob	UTSW	12	8,065,990 (GRCm39)	missense	probably damaging	0.99
R9016:Apob	UTSW	12	8,035,408 (GRCm39)	splice site	silent
R9020:Apob	UTSW	12	8,063,999 (GRCm39)	missense	probably damaging	1.00
R9037:Apob	UTSW	12	8,066,501 (GRCm39)	missense	probably benign	0.15
R9053:Apob	UTSW	12	8,058,954 (GRCm39)	missense	possibly damaging	0.72
R9062:Apob	UTSW	12	8,058,046 (GRCm39)	missense	possibly damaging	0.91
R9142:Apob	UTSW	12	8,062,705 (GRCm39)	missense	possibly damaging	0.95
R9180:Apob	UTSW	12	8,047,925 (GRCm39)	missense	probably damaging	1.00
R9205:Apob	UTSW	12	8,030,635 (GRCm39)	missense	probably damaging	0.99
R9248:Apob	UTSW	12	8,065,231 (GRCm39)	nonsense	probably null
R9277:Apob	UTSW	12	8,061,183 (GRCm39)	missense	probably benign	0.01
R9305:Apob	UTSW	12	8,058,053 (GRCm39)	missense	probably benign	0.04
R9358:Apob	UTSW	12	8,060,833 (GRCm39)	missense	probably benign	0.14
R9375:Apob	UTSW	12	8,029,261 (GRCm39)	missense	possibly damaging	0.91
R9385:Apob	UTSW	12	8,056,399 (GRCm39)	missense	possibly damaging	0.91
R9386:Apob	UTSW	12	8,056,629 (GRCm39)	missense	probably damaging	0.99
R9392:Apob	UTSW	12	8,057,098 (GRCm39)	missense	probably benign	0.45
R9470:Apob	UTSW	12	8,039,219 (GRCm39)	missense	possibly damaging	0.94
R9523:Apob	UTSW	12	8,052,069 (GRCm39)	missense	probably damaging	1.00
R9545:Apob	UTSW	12	8,033,890 (GRCm39)	missense	possibly damaging	0.81
R9629:Apob	UTSW	12	8,059,054 (GRCm39)	missense	probably damaging	1.00
R9702:Apob	UTSW	12	8,057,559 (GRCm39)	missense	probably damaging	0.96
R9703:Apob	UTSW	12	8,030,507 (GRCm39)	missense	probably damaging	0.99
R9719:Apob	UTSW	12	8,065,464 (GRCm39)	missense	probably benign	0.15
R9726:Apob	UTSW	12	8,056,926 (GRCm39)	missense	probably damaging	0.99
R9729:Apob	UTSW	12	8,066,125 (GRCm39)	missense	probably damaging	0.99
X0027:Apob	UTSW	12	8,057,975 (GRCm39)	missense	probably benign
Z1088:Apob	UTSW	12	8,062,936 (GRCm39)	missense	possibly damaging	0.95
Z1088:Apob	UTSW	12	8,055,945 (GRCm39)	nonsense	probably null
Z1088:Apob	UTSW	12	8,055,074 (GRCm39)	missense	possibly damaging	0.91
Z1176:Apob	UTSW	12	8,054,978 (GRCm39)	missense	probably benign	0.00
Z1176:Apob	UTSW	12	8,048,011 (GRCm39)	missense	probably damaging	1.00
Z1177:Apob	UTSW	12	8,065,249 (GRCm39)	frame shift	probably null
Z1177:Apob	UTSW	12	8,038,765 (GRCm39)	missense	probably benign	0.43

Predicted Primers

PCR Primer
(F):5'- TCTCCGTGCTCTCCAAGAAG -3'
(R):5'- TGTGAAGATCAGGAACGTGTAAATC -3'

Sequencing Primer
(F):5'- TCCGTGCTCTCCAAGAAGGTAAC -3'
(R):5'- GATCAGGAACGTGTAAATCTTTGAG -3'

Posted On

2019-10-24