Incidental Mutation 'R9667:Gpr158'
| ID |
727818 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr158
|
| Ensembl Gene |
ENSMUSG00000045967 |
| Gene Name |
G protein-coupled receptor 158 |
| Synonyms |
5330427M13Rik |
| MMRRC Submission |
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R9667 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
21372378-21835355 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 21830054 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Valine
at position 700
(I700V)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000049708
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000055946]
|
| AlphaFold |
Q8C419 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000055946
AA Change: I700V
PolyPhen 2
Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: I700V
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
110 |
125 |
N/A |
INTRINSIC |
|
SCOP:d1edmb_
|
313 |
359 |
5e-4 |
SMART |
|
Blast:EGF
|
318 |
365 |
2e-27 |
BLAST |
|
Pfam:7tm_3
|
426 |
669 |
1.2e-35 |
PFAM |
|
low complexity region
|
840 |
863 |
N/A |
INTRINSIC |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.7%
- 20x: 98.8%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 88 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930563M21Rik |
G |
A |
9: 55,890,645 (GRCm39) |
Q355* |
probably null |
Het |
| Aacs |
A |
T |
5: 125,580,691 (GRCm39) |
N255I |
possibly damaging |
Het |
| Abcf2 |
A |
G |
5: 24,779,185 (GRCm39) |
L121P |
probably damaging |
Het |
| Amigo1 |
A |
G |
3: 108,095,034 (GRCm39) |
I178V |
probably benign |
Het |
| Ankrd13a |
T |
A |
5: 114,933,793 (GRCm39) |
N262K |
probably damaging |
Het |
| Apba2 |
A |
G |
7: 64,345,062 (GRCm39) |
E84G |
possibly damaging |
Het |
| Arhgap35 |
A |
T |
7: 16,296,914 (GRCm39) |
L717* |
probably null |
Het |
| Aspa |
A |
T |
11: 73,199,625 (GRCm39) |
C217* |
probably null |
Het |
| Atp5mc3 |
A |
G |
2: 73,739,567 (GRCm39) |
I91T |
probably damaging |
Het |
| Birc6 |
T |
C |
17: 75,004,420 (GRCm39) |
M4763T |
possibly damaging |
Het |
| Cc2d2b |
A |
G |
19: 40,753,927 (GRCm39) |
Y150C |
unknown |
Het |
| Ccdc180 |
C |
T |
4: 45,920,861 (GRCm39) |
Q936* |
probably null |
Het |
| Cdc42bpg |
A |
G |
19: 6,370,115 (GRCm39) |
E1103G |
probably benign |
Het |
| Cdca2 |
T |
C |
14: 67,915,003 (GRCm39) |
Q752R |
probably benign |
Het |
| Ces1b |
A |
T |
8: 93,791,637 (GRCm39) |
S321T |
probably benign |
Het |
| Cfap70 |
A |
T |
14: 20,490,690 (GRCm39) |
|
probably null |
Het |
| Cpox |
C |
T |
16: 58,490,984 (GRCm39) |
T65I |
possibly damaging |
Het |
| Crocc |
C |
T |
4: 140,748,988 (GRCm39) |
E1606K |
probably damaging |
Het |
| Cryzl2 |
T |
G |
1: 157,316,038 (GRCm39) |
M337R |
probably benign |
Het |
| Cyp4a29 |
C |
A |
4: 115,111,630 (GRCm39) |
A469D |
probably damaging |
Het |
| Dnaaf1 |
T |
A |
8: 120,306,043 (GRCm39) |
Y107N |
possibly damaging |
Het |
| Dpp6 |
T |
A |
5: 27,930,604 (GRCm39) |
I812N |
probably damaging |
Het |
| Elovl5 |
A |
G |
9: 77,889,947 (GRCm39) |
T253A |
possibly damaging |
Het |
| Fancd2 |
T |
A |
6: 113,530,717 (GRCm39) |
L450* |
probably null |
Het |
| Fbxo31 |
C |
T |
8: 122,305,208 (GRCm39) |
R96H |
probably damaging |
Het |
| Gls |
A |
T |
1: 52,230,036 (GRCm39) |
|
probably null |
Het |
| Gm3045 |
G |
A |
13: 56,577,253 (GRCm39) |
E134K |
possibly damaging |
Het |
| H2-Aa |
T |
C |
17: 34,502,295 (GRCm39) |
I209V |
probably benign |
Het |
| Ifnar2 |
A |
G |
16: 91,184,984 (GRCm39) |
D125G |
probably benign |
Het |
| Igsf8 |
C |
A |
1: 172,145,319 (GRCm39) |
D278E |
possibly damaging |
Het |
| Inpp5d |
T |
C |
1: 87,623,128 (GRCm39) |
I407T |
probably damaging |
Het |
| Iqcd |
A |
G |
5: 120,744,737 (GRCm39) |
E355G |
probably damaging |
Het |
| Klra2 |
T |
A |
6: 131,219,836 (GRCm39) |
D115V |
probably benign |
Het |
| Kmt2b |
T |
C |
7: 30,287,784 (GRCm39) |
E120G |
unknown |
Het |
| Lhx6 |
A |
G |
2: 35,980,979 (GRCm39) |
I321T |
possibly damaging |
Het |
| Lmna |
CAGCACGGTGCGTGAGC |
CAGC |
3: 88,389,857 (GRCm39) |
|
probably null |
Het |
| Lztr1 |
T |
C |
16: 17,327,000 (GRCm39) |
Y37H |
probably damaging |
Het |
| Malrd1 |
A |
T |
2: 15,570,026 (GRCm39) |
|
probably null |
Het |
| Mtmr3 |
C |
T |
11: 4,470,890 (GRCm39) |
V79I |
probably damaging |
Het |
| Nbr1 |
A |
G |
11: 101,451,261 (GRCm39) |
I56M |
possibly damaging |
Het |
| Ncor2 |
C |
T |
5: 125,125,545 (GRCm39) |
A580T |
unknown |
Het |
| Ndst3 |
A |
G |
3: 123,353,866 (GRCm39) |
I601T |
possibly damaging |
Het |
| Nectin4 |
C |
A |
1: 171,210,165 (GRCm39) |
P219Q |
probably damaging |
Het |
| Nfatc4 |
T |
C |
14: 56,066,964 (GRCm39) |
V501A |
probably benign |
Het |
| Nisch |
T |
C |
14: 30,895,646 (GRCm39) |
T1015A |
probably damaging |
Het |
| Nr2c1 |
T |
C |
10: 94,017,479 (GRCm39) |
|
probably null |
Het |
| Nrg2 |
T |
C |
18: 36,165,430 (GRCm39) |
E394G |
probably benign |
Het |
| Oas1e |
A |
G |
5: 120,932,347 (GRCm39) |
F99L |
probably benign |
Het |
| Oaz3 |
A |
T |
3: 94,341,835 (GRCm39) |
Y178* |
probably null |
Het |
| Or1e35 |
A |
G |
11: 73,798,097 (GRCm39) |
S74P |
possibly damaging |
Het |
| Or6c69b |
A |
C |
10: 129,627,022 (GRCm39) |
S145R |
probably damaging |
Het |
| Pcdhb20 |
C |
T |
18: 37,637,839 (GRCm39) |
H122Y |
probably benign |
Het |
| Pdcd2 |
T |
A |
17: 15,747,535 (GRCm39) |
M1L |
probably damaging |
Het |
| Phf11 |
T |
C |
14: 59,482,240 (GRCm39) |
N171S |
probably benign |
Het |
| Plcd1 |
C |
A |
9: 118,901,698 (GRCm39) |
G609W |
probably damaging |
Het |
| Plscr3 |
T |
C |
11: 69,738,631 (GRCm39) |
F98L |
probably benign |
Het |
| Ppp1r8 |
T |
C |
4: 132,570,407 (GRCm39) |
N6S |
probably benign |
Het |
| Prpf38b |
G |
A |
3: 108,818,859 (GRCm39) |
|
probably benign |
Het |
| Rin2 |
T |
A |
2: 145,702,202 (GRCm39) |
N299K |
possibly damaging |
Het |
| Serpina1d |
T |
G |
12: 103,734,299 (GRCm39) |
T2P |
probably benign |
Het |
| Slc22a16 |
A |
G |
10: 40,461,125 (GRCm39) |
D330G |
probably benign |
Het |
| Slit1 |
G |
A |
19: 41,731,832 (GRCm39) |
Q6* |
probably null |
Het |
| Spaca7b |
T |
A |
8: 11,705,681 (GRCm39) |
E142V |
probably benign |
Het |
| Spag9 |
T |
C |
11: 93,887,119 (GRCm39) |
V8A |
possibly damaging |
Het |
| Spsb3 |
C |
T |
17: 25,105,784 (GRCm39) |
T44I |
unknown |
Het |
| Srrt |
T |
C |
5: 137,295,732 (GRCm39) |
Y563C |
probably damaging |
Het |
| Syne2 |
A |
G |
12: 75,926,951 (GRCm39) |
D69G |
probably damaging |
Het |
| Tcf12 |
A |
G |
9: 71,792,443 (GRCm39) |
V80A |
probably benign |
Het |
| Tdpoz3 |
G |
A |
3: 93,733,336 (GRCm39) |
D4N |
possibly damaging |
Het |
| Tekt2 |
C |
T |
4: 126,217,444 (GRCm39) |
R207H |
probably damaging |
Het |
| Tmem132d |
G |
A |
5: 128,061,375 (GRCm39) |
T409I |
possibly damaging |
Het |
| Tmem245 |
G |
T |
4: 56,947,119 (GRCm39) |
T98K |
probably damaging |
Het |
| Tmem70 |
A |
C |
1: 16,735,659 (GRCm39) |
E43A |
probably benign |
Het |
| Trim25 |
A |
T |
11: 88,907,188 (GRCm39) |
I516F |
probably damaging |
Het |
| Trim3 |
G |
A |
7: 105,267,455 (GRCm39) |
S308L |
possibly damaging |
Het |
| Ttll9 |
C |
T |
2: 152,831,989 (GRCm39) |
R189* |
probably null |
Het |
| Ttpal |
T |
C |
2: 163,449,596 (GRCm39) |
|
probably null |
Het |
| Ubl4b |
A |
T |
3: 107,461,911 (GRCm39) |
H116Q |
probably benign |
Het |
| Unc80 |
C |
A |
1: 66,651,287 (GRCm39) |
T1544K |
possibly damaging |
Het |
| Usp2 |
T |
C |
9: 44,003,487 (GRCm39) |
|
probably null |
Het |
| Usp25 |
T |
C |
16: 76,874,123 (GRCm39) |
|
probably null |
Het |
| Vmn1r167 |
C |
T |
7: 23,204,990 (GRCm39) |
V9I |
probably benign |
Het |
| Vmn2r87 |
A |
G |
10: 130,314,776 (GRCm39) |
I270T |
probably damaging |
Het |
| Vps35l |
G |
A |
7: 118,348,915 (GRCm39) |
|
probably null |
Het |
| Wdr24 |
A |
G |
17: 26,046,301 (GRCm39) |
D515G |
possibly damaging |
Het |
| Wdr81 |
T |
C |
11: 75,341,650 (GRCm39) |
T87A |
|
Het |
| Zcchc14 |
A |
T |
8: 122,331,863 (GRCm39) |
L500Q |
unknown |
Het |
| Zzef1 |
A |
G |
11: 72,758,786 (GRCm39) |
T1242A |
probably benign |
Het |
|
| Other mutations in Gpr158 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00340:Gpr158
|
APN |
2 |
21,373,494 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00469:Gpr158
|
APN |
2 |
21,751,606 (GRCm39) |
splice site |
probably benign |
|
|
IGL00706:Gpr158
|
APN |
2 |
21,751,584 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00780:Gpr158
|
APN |
2 |
21,831,629 (GRCm39) |
nonsense |
probably null |
|
|
IGL00885:Gpr158
|
APN |
2 |
21,653,832 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01339:Gpr158
|
APN |
2 |
21,373,842 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
IGL01368:Gpr158
|
APN |
2 |
21,831,909 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02141:Gpr158
|
APN |
2 |
21,788,101 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02455:Gpr158
|
APN |
2 |
21,373,511 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02554:Gpr158
|
APN |
2 |
21,831,407 (GRCm39) |
missense |
probably benign |
|
|
IGL02681:Gpr158
|
APN |
2 |
21,820,441 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02752:Gpr158
|
APN |
2 |
21,831,638 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
IGL02756:Gpr158
|
APN |
2 |
21,831,890 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
IGL03181:Gpr158
|
APN |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL03258:Gpr158
|
APN |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03386:Gpr158
|
APN |
2 |
21,831,057 (GRCm39) |
missense |
probably damaging |
1.00 |
|
PIT4810001:Gpr158
|
UTSW |
2 |
21,831,682 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0071:Gpr158
|
UTSW |
2 |
21,815,479 (GRCm39) |
missense |
probably benign |
0.08 |
|
R0081:Gpr158
|
UTSW |
2 |
21,831,528 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0528:Gpr158
|
UTSW |
2 |
21,830,019 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0560:Gpr158
|
UTSW |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0603:Gpr158
|
UTSW |
2 |
21,820,480 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R1560:Gpr158
|
UTSW |
2 |
21,831,125 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1561:Gpr158
|
UTSW |
2 |
21,820,505 (GRCm39) |
splice site |
probably null |
|
|
R1609:Gpr158
|
UTSW |
2 |
21,788,104 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1741:Gpr158
|
UTSW |
2 |
21,832,359 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1827:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R1854:Gpr158
|
UTSW |
2 |
21,373,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1871:Gpr158
|
UTSW |
2 |
21,820,426 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2151:Gpr158
|
UTSW |
2 |
21,832,325 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R2273:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R2275:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R3004:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R3151:Gpr158
|
UTSW |
2 |
21,581,771 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R3943:Gpr158
|
UTSW |
2 |
21,373,370 (GRCm39) |
missense |
possibly damaging |
0.65 |
|
R4238:Gpr158
|
UTSW |
2 |
21,373,362 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4379:Gpr158
|
UTSW |
2 |
21,830,025 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4381:Gpr158
|
UTSW |
2 |
21,832,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4464:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4467:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4496:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4506:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4530:Gpr158
|
UTSW |
2 |
21,373,811 (GRCm39) |
missense |
probably benign |
0.03 |
|
R4646:Gpr158
|
UTSW |
2 |
21,831,864 (GRCm39) |
missense |
probably benign |
|
|
R4798:Gpr158
|
UTSW |
2 |
21,787,993 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4882:Gpr158
|
UTSW |
2 |
21,830,059 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4943:Gpr158
|
UTSW |
2 |
21,831,968 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5334:Gpr158
|
UTSW |
2 |
21,832,316 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5560:Gpr158
|
UTSW |
2 |
21,831,101 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R5600:Gpr158
|
UTSW |
2 |
21,832,046 (GRCm39) |
missense |
probably benign |
|
|
R5637:Gpr158
|
UTSW |
2 |
21,788,083 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5701:Gpr158
|
UTSW |
2 |
21,751,520 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5744:Gpr158
|
UTSW |
2 |
21,373,331 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5911:Gpr158
|
UTSW |
2 |
21,373,932 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R5991:Gpr158
|
UTSW |
2 |
21,373,319 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6200:Gpr158
|
UTSW |
2 |
21,404,227 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6306:Gpr158
|
UTSW |
2 |
21,820,422 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R6324:Gpr158
|
UTSW |
2 |
21,815,365 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6384:Gpr158
|
UTSW |
2 |
21,831,099 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6698:Gpr158
|
UTSW |
2 |
21,831,921 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6997:Gpr158
|
UTSW |
2 |
21,653,802 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R7086:Gpr158
|
UTSW |
2 |
21,831,386 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7175:Gpr158
|
UTSW |
2 |
21,373,113 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7197:Gpr158
|
UTSW |
2 |
21,815,412 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7293:Gpr158
|
UTSW |
2 |
21,581,750 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R7427:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R7515:Gpr158
|
UTSW |
2 |
21,373,092 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7730:Gpr158
|
UTSW |
2 |
21,831,158 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8122:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R8311:Gpr158
|
UTSW |
2 |
21,373,701 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8754:Gpr158
|
UTSW |
2 |
21,581,693 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8782:Gpr158
|
UTSW |
2 |
21,404,149 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8792:Gpr158
|
UTSW |
2 |
21,558,137 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8842:Gpr158
|
UTSW |
2 |
21,581,751 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R9009:Gpr158
|
UTSW |
2 |
21,581,760 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9102:Gpr158
|
UTSW |
2 |
21,830,078 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9150:Gpr158
|
UTSW |
2 |
21,831,251 (GRCm39) |
missense |
probably benign |
0.17 |
|
R9254:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9317:Gpr158
|
UTSW |
2 |
21,832,037 (GRCm39) |
missense |
probably benign |
|
|
R9379:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9428:Gpr158
|
UTSW |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
|
|
R9497:Gpr158
|
UTSW |
2 |
21,831,825 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9681:Gpr158
|
UTSW |
2 |
21,831,315 (GRCm39) |
missense |
probably damaging |
0.99 |
|
X0062:Gpr158
|
UTSW |
2 |
21,831,180 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1176:Gpr158
|
UTSW |
2 |
21,815,501 (GRCm39) |
critical splice donor site |
probably null |
|
|
Z1177:Gpr158
|
UTSW |
2 |
21,832,083 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
| Predicted Primers |
PCR Primer
(F):5'- GAACCTTAGAAAATGCTGCACTG -3'
(R):5'- GTTGAGACAGTTTTGTGAAGCAC -3'
Sequencing Primer
(F):5'- AATGCTGCACTGTCTAGCAATGG -3'
(R):5'- GTGGTTCTTTCAGTCTCTCACAG -3'
|
| Posted On |
2022-10-06 |
Incidental Mutation