Mutagenetix > Incidental Mutation

Incidental Mutation 'R2211:Rnpepl1'

239345

Institutional Source

Beutler Lab

Gene Symbol

Rnpepl1

Ensembl Gene

ENSMUSG00000026269

Gene Name

arginyl aminopeptidase (aminopeptidase B)-like 1

Synonyms

MMRRC Submission

040213-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2211 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

92910783-92924384 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 92916380 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 278 (L278F)

Ref Sequence

ENSEMBL: ENSMUSP00000027487 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027487] [ENSMUST00000178116]

AlphaFold

G5E872

Predicted Effect

probably damaging
Transcript: ENSMUST00000027487
AA Change: L278F

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000027487
Gene: ENSMUSG00000026269
AA Change: L278F

Domain	Start	End	E-Value	Type
low complexity region	10	27	N/A	INTRINSIC
Pfam:Peptidase_M1	36	440	3e-58	PFAM
Leuk-A4-hydro_C	523	668	1.31e-46	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000178116

SMART Domains

Protein: ENSMUSP00000136080
Gene: ENSMUSG00000026269

Domain	Start	End	E-Value	Type
Pfam:Peptidase_M1	5	170	1.2e-13	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000178361

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000178662

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000178778

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000178816

Predicted Effect

probably benign
Transcript: ENSMUST00000179127

Predicted Effect

probably benign
Transcript: ENSMUST00000179531

Predicted Effect

probably benign
Transcript: ENSMUST00000179837

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000179854

Predicted Effect

probably benign
Transcript: ENSMUST00000179993

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180306

Meta Mutation Damage Score

0.1370

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430078G23Rik	T	C	8: 3,387,680	S268P	possibly damaging	Het
Adam18	A	G	8: 24,628,155	S34P	probably damaging	Het
Adam23	T	A	1: 63,573,129		probably benign	Het
Adcy10	A	T	1: 165,518,212	I277F	probably damaging	Het
Arfgef3	A	T	10: 18,592,245	S1736T	possibly damaging	Het
Arhgap21	A	T	2: 20,881,640	M242K	possibly damaging	Het
Astn1	A	G	1: 158,657,306	R4G	probably benign	Het
AU041133	G	A	10: 82,150,921	C135Y	probably damaging	Het
Cdc42bpb	A	G	12: 111,301,854	V53A	probably benign	Het
Cdh23	T	C	10: 60,466,004	D428G	possibly damaging	Het
Cebpa	T	C	7: 35,120,466	S350P	probably damaging	Het
Cftr	A	G	6: 18,214,280	M152V	probably null	Het
Cpa4	A	G	6: 30,583,650	N255S	possibly damaging	Het
Ddx51	T	A	5: 110,655,768	D343E	probably damaging	Het
Dnah7a	T	C	1: 53,479,773	I2942V	probably benign	Het
Dnajc1	G	T	2: 18,392,475	A9E	probably damaging	Het
Dpysl5	G	A	5: 30,791,597	D399N	probably damaging	Het
Edem3	A	G	1: 151,804,702	D526G	possibly damaging	Het
Emc10	G	A	7: 44,493,192	R109W	probably damaging	Het
Etaa1	G	A	11: 17,952,686	Q84*	probably null	Het
Fabp3	C	T	4: 130,312,387	T57I	probably benign	Het
Fam149a	G	A	8: 45,341,009	T674I	probably damaging	Het
Fam98a	A	G	17: 75,538,945		probably null	Het
Fat4	A	G	3: 38,891,527	N1523S	possibly damaging	Het
Fbxw10	A	G	11: 62,867,535	T529A	probably damaging	Het
Gzf1	C	T	2: 148,684,950	A447V	probably damaging	Het
Hap1	G	A	11: 100,354,724	T138M	probably benign	Het
Hic1	T	A	11: 75,169,384	R46W	possibly damaging	Het
Id4	T	A	13: 48,261,802	L102Q	probably damaging	Het
Il3ra	T	C	14: 14,355,029	C271R	probably benign	Het
Ints4	T	C	7: 97,509,750	I443T	possibly damaging	Het
Lmln	A	G	16: 33,109,778	E535G	probably benign	Het
Lrrtm4	A	G	6: 80,022,640	H345R	probably benign	Het
Ltbp3	T	C	19: 5,753,962	I834T	possibly damaging	Het
Mnd1	C	A	3: 84,134,109	C62F	probably benign	Het
Mpp5	A	T	12: 78,797,248	K75N	possibly damaging	Het
Ms4a18	C	A	19: 10,997,305	V341L	probably benign	Het
Nbr1	T	C	11: 101,567,264		probably null	Het
Nf1	T	C	11: 79,444,064	M914T	probably benign	Het
Notch3	T	A	17: 32,147,978	H861L	probably benign	Het
Nupl1	A	G	14: 60,232,640	F341L	probably damaging	Het
Ogfod2	C	A	5: 124,112,780		probably null	Het
Oit3	T	C	10: 59,428,070	D414G	probably damaging	Het
Olfr1079	T	C	2: 86,538,513	Y132C	probably damaging	Het
Olfr1497	T	A	19: 13,795,369	M81L	probably benign	Het
Olfr787	A	T	10: 129,462,940	K88M	probably damaging	Het
Olfr804	A	G	10: 129,705,451	H191R	probably benign	Het
Pcbp4	C	A	9: 106,460,734	H74Q	probably benign	Het
Pip5k1b	A	T	19: 24,378,850	D241E	probably damaging	Het
Plcb2	T	C	2: 118,723,534	D102G	probably benign	Het
Plekha5	G	A	6: 140,525,861	E4K	possibly damaging	Het
Ppargc1a	A	C	5: 51,474,259	S343A	possibly damaging	Het
Ppwd1	G	A	13: 104,207,142	S585L	probably benign	Het
Rarg	T	C	15: 102,239,524	N284S	probably benign	Het
Rp1	A	C	1: 4,348,139	S917A	probably damaging	Het
Rsf1	ATGGCG	ATGGCGAGGGTGGCG	7: 97,579,904		probably benign	Het
Rsph3b	T	C	17: 6,941,740	S189G	probably benign	Het
Sec31b	A	T	19: 44,523,150	L604Q	probably damaging	Het
Sema6b	T	A	17: 56,124,741	I641F	probably benign	Het
Slc35f5	A	T	1: 125,579,264	I309F	possibly damaging	Het
Smarca4	A	G	9: 21,686,029	E1360G	probably damaging	Het
Spata31d1c	T	C	13: 65,035,939	S432P	probably benign	Het
Spsb3	T	C	17: 24,890,937		probably null	Het
Sptbn4	T	C	7: 27,367,609	D1960G	probably damaging	Het
Srgap1	A	G	10: 121,853,740	V345A	possibly damaging	Het
Tiam2	C	A	17: 3,414,918	C307*	probably null	Het
Trim43b	T	A	9: 89,085,249	T444S	possibly damaging	Het
Trmt13	A	G	3: 116,594,754	I11T	probably benign	Het
Ylpm1	A	T	12: 85,044,378	R1073*	probably null	Het
Zfp524	T	C	7: 5,017,919	S149P	probably damaging	Het
Zfp777	T	C	6: 48,043,885	I312V	possibly damaging	Het

Other mutations in Rnpepl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01152:Rnpepl1	APN	1	92915899	missense	possibly damaging	0.69
IGL01291:Rnpepl1	APN	1	92919746	missense	probably benign
IGL02266:Rnpepl1	APN	1	92916889	missense	probably damaging	1.00
IGL02481:Rnpepl1	APN	1	92915907	missense	probably damaging	1.00
IGL02483:Rnpepl1	APN	1	92915907	missense	probably damaging	1.00
IGL03377:Rnpepl1	APN	1	92919231	missense	probably benign	0.01
ANU05:Rnpepl1	UTSW	1	92919746	missense	probably benign
R0069:Rnpepl1	UTSW	1	92918898	missense	possibly damaging	0.91
R0409:Rnpepl1	UTSW	1	92915860	missense	probably damaging	1.00
R0479:Rnpepl1	UTSW	1	92918865	unclassified	probably benign
R1155:Rnpepl1	UTSW	1	92916887	missense	probably damaging	1.00
R1170:Rnpepl1	UTSW	1	92919195	missense	possibly damaging	0.56
R1397:Rnpepl1	UTSW	1	92917159	missense	probably damaging	1.00
R1601:Rnpepl1	UTSW	1	92917222	missense	possibly damaging	0.95
R2184:Rnpepl1	UTSW	1	92916823	missense	probably benign	0.43
R2187:Rnpepl1	UTSW	1	92916895	missense	probably null	1.00
R2902:Rnpepl1	UTSW	1	92916380	missense	probably damaging	1.00
R3105:Rnpepl1	UTSW	1	92916380	missense	probably damaging	1.00
R3196:Rnpepl1	UTSW	1	92917159	missense	probably damaging	1.00
R3439:Rnpepl1	UTSW	1	92916940	missense	possibly damaging	0.94
R4887:Rnpepl1	UTSW	1	92915113	missense	probably damaging	1.00
R4966:Rnpepl1	UTSW	1	92916761	missense	probably damaging	1.00
R5212:Rnpepl1	UTSW	1	92911323	missense	probably benign	0.03
R5214:Rnpepl1	UTSW	1	92919279	missense	probably benign	0.01
R5385:Rnpepl1	UTSW	1	92917192	missense	probably damaging	1.00
R5655:Rnpepl1	UTSW	1	92919310	missense	probably damaging	1.00
R5694:Rnpepl1	UTSW	1	92918941	missense	probably benign	0.03
R5940:Rnpepl1	UTSW	1	92917712	missense	probably damaging	1.00
R6046:Rnpepl1	UTSW	1	92916821	missense	probably damaging	1.00
R6086:Rnpepl1	UTSW	1	92917681	missense	probably damaging	1.00
R6104:Rnpepl1	UTSW	1	92915884	missense	probably benign
R6349:Rnpepl1	UTSW	1	92919841	missense	probably damaging	1.00
R7381:Rnpepl1	UTSW	1	92919195	missense	possibly damaging	0.56
R7402:Rnpepl1	UTSW	1	92919650	missense	probably benign	0.01
R7474:Rnpepl1	UTSW	1	92918972	missense	probably benign	0.14
R7714:Rnpepl1	UTSW	1	92917168	missense	probably damaging	1.00
R9408:Rnpepl1	UTSW	1	92917702	missense	probably benign	0.19
R9566:Rnpepl1	UTSW	1	92919746	missense
R9591:Rnpepl1	UTSW	1	92919587	missense	probably damaging	1.00
R9773:Rnpepl1	UTSW	1	92919837	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CACCTTGGGTTCCTTAAGTAGTTC -3'
(R):5'- ATGTATGCTTCACCTGGGATGG -3'

Sequencing Primer
(F):5'- CCCCTACCTGTTGTGTGAAGGAG -3'
(R):5'- GAAGTCCCAATCTAGCCCTATGTC -3'

Posted On

2014-10-15