Mutagenetix > Incidental Mutation

Incidental Mutation 'R3729:Vmn2r14'

270872

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r14

Ensembl Gene

ENSMUSG00000091059

Gene Name

vomeronasal 2, receptor 14

Synonyms

EG231591

MMRRC Submission

040719-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.526) question?

Stock #

R3729 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109362822-109372488 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 109364095 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 607 (Y607C)

Ref Sequence

ENSEMBL: ENSMUSP00000128015 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170341]

AlphaFold

E9Q759

Predicted Effect

probably damaging
Transcript: ENSMUST00000170341
AA Change: Y607C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000128015
Gene: ENSMUSG00000091059
AA Change: Y607C

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.3e-31	PFAM
Pfam:NCD3G	507	561	1.1e-17	PFAM
Pfam:7tm_3	594	829	1.2e-55	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	A	G	5: 114,345,409 (GRCm39)	D954G	probably damaging	Het
Acvr1	T	C	2: 58,352,925 (GRCm39)	K345R	probably null	Het
Aldoart2	A	G	12: 55,613,104 (GRCm39)	Y343C	probably damaging	Het
Arpp21	T	A	9: 111,895,047 (GRCm39)	Y739F	possibly damaging	Het
Cachd1	C	A	4: 100,832,077 (GRCm39)	Y746*	probably null	Het
Card14	T	C	11: 119,224,758 (GRCm39)	V595A	probably damaging	Het
Dhx33	T	C	11: 70,879,978 (GRCm39)	D344G	probably benign	Het
Dnah12	T	A	14: 26,427,220 (GRCm39)	D171E	probably benign	Het
Fat3	A	G	9: 16,158,337 (GRCm39)		probably benign	Het
Fmnl3	A	G	15: 99,219,745 (GRCm39)	F668L	probably damaging	Het
Frmpd4	G	A	X: 166,269,803 (GRCm39)	T493M	probably damaging	Het
Gcn1	A	T	5: 115,721,453 (GRCm39)		probably benign	Het
Hspbp1	G	T	7: 4,680,808 (GRCm39)	Q223K	probably damaging	Het
Ighv1-19	C	A	12: 114,672,497 (GRCm39)	C40F	probably damaging	Het
Igkv4-90	A	G	6: 68,784,665 (GRCm39)	F10L	probably benign	Het
Kctd5	T	C	17: 24,278,212 (GRCm39)	D146G	probably benign	Het
Kif4-ps	A	G	12: 101,112,369 (GRCm39)	E166G	probably damaging	Het
Lemd3	T	C	10: 120,763,920 (GRCm39)	D780G	probably damaging	Het
Manba	G	A	3: 135,260,611 (GRCm39)	V599I	probably benign	Het
Map2	A	G	1: 66,451,605 (GRCm39)	E247G	possibly damaging	Het
Mfsd1	T	C	3: 67,490,298 (GRCm39)	F50L	probably benign	Het
Mier2	T	C	10: 79,380,876 (GRCm39)		probably benign	Het
Mslnl	G	A	17: 25,961,908 (GRCm39)	V128M	probably damaging	Het
Nol10	A	T	12: 17,474,674 (GRCm39)	K622I	probably benign	Het
Or5p62	A	T	7: 107,771,516 (GRCm39)	I145N	probably benign	Het
Or8d2b	T	C	9: 38,788,547 (GRCm39)	F25S	probably damaging	Het
Phtf1	A	G	3: 103,893,095 (GRCm39)	M120V	probably benign	Het
Prrx1	A	G	1: 163,089,446 (GRCm39)	L127P	probably damaging	Het
Rbsn	T	C	6: 92,168,316 (GRCm39)	M373V	possibly damaging	Het
Sec24b	T	C	3: 129,827,482 (GRCm39)	K203R	possibly damaging	Het
Shroom3	G	T	5: 93,090,945 (GRCm39)	V1151F	probably damaging	Het
Slc16a10	G	C	10: 39,932,620 (GRCm39)	H314D	possibly damaging	Het
Slc4a7	A	T	14: 14,729,276 (GRCm38)	E47D	probably damaging	Het
Taf12	C	A	4: 132,010,265 (GRCm39)	P78Q	probably damaging	Het
Tnn	A	G	1: 159,973,810 (GRCm39)	C186R	probably damaging	Het
Trim46	T	A	3: 89,142,256 (GRCm39)	T721S	probably benign	Het
Trip4	T	C	9: 65,788,224 (GRCm39)	N74D	possibly damaging	Het
Tssk5	C	T	15: 76,257,096 (GRCm39)	R262H	probably benign	Het
Vegfa	T	C	17: 46,335,446 (GRCm39)	I105V	possibly damaging	Het
Wdr83	G	T	8: 85,806,968 (GRCm39)	H66Q	probably damaging	Het
Zfp280b	A	G	10: 75,874,936 (GRCm39)	T272A	probably benign	Het
Zfp960	T	A	17: 17,308,633 (GRCm39)	L449H	probably damaging	Het

Other mutations in Vmn2r14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01472:Vmn2r14	APN	5	109,364,180 (GRCm39)	nonsense	probably null
IGL01504:Vmn2r14	APN	5	109,369,285 (GRCm39)	missense	probably benign	0.01
IGL01828:Vmn2r14	APN	5	109,372,443 (GRCm39)	missense	possibly damaging	0.71
IGL02093:Vmn2r14	APN	5	109,368,275 (GRCm39)	missense	possibly damaging	0.94
IGL02103:Vmn2r14	APN	5	109,372,349 (GRCm39)	missense	probably damaging	0.96
IGL02123:Vmn2r14	APN	5	109,367,933 (GRCm39)	missense	probably damaging	1.00
IGL02145:Vmn2r14	APN	5	109,368,454 (GRCm39)	nonsense	probably null
IGL02676:Vmn2r14	APN	5	109,367,882 (GRCm39)	missense	probably benign	0.03
IGL02720:Vmn2r14	APN	5	109,369,305 (GRCm39)	missense	probably damaging	1.00
IGL02877:Vmn2r14	APN	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
IGL02974:Vmn2r14	APN	5	109,369,292 (GRCm39)	missense	possibly damaging	0.55
IGL03151:Vmn2r14	APN	5	109,364,260 (GRCm39)	missense	probably damaging	1.00
IGL03297:Vmn2r14	APN	5	109,363,973 (GRCm39)	missense	probably damaging	1.00
IGL03386:Vmn2r14	APN	5	109,368,350 (GRCm39)	missense	possibly damaging	0.90
IGL03394:Vmn2r14	APN	5	109,367,702 (GRCm39)	missense	probably null	0.83
ANU74:Vmn2r14	UTSW	5	109,366,910 (GRCm39)	missense	probably benign	0.00
R0316:Vmn2r14	UTSW	5	109,366,762 (GRCm39)	missense	probably benign	0.07
R0755:Vmn2r14	UTSW	5	109,364,226 (GRCm39)	missense	possibly damaging	0.81
R1219:Vmn2r14	UTSW	5	109,372,440 (GRCm39)	missense	probably benign	0.17
R1321:Vmn2r14	UTSW	5	109,364,117 (GRCm39)	missense	probably benign	0.08
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1509:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R1551:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	probably damaging	1.00
R1628:Vmn2r14	UTSW	5	109,367,838 (GRCm39)	missense	probably benign	0.00
R1668:Vmn2r14	UTSW	5	109,366,913 (GRCm39)	nonsense	probably null
R2013:Vmn2r14	UTSW	5	109,369,109 (GRCm39)	missense	probably benign	0.00
R2201:Vmn2r14	UTSW	5	109,366,698 (GRCm39)	splice site	probably null
R2417:Vmn2r14	UTSW	5	109,372,329 (GRCm39)	missense	probably benign	0.00
R3029:Vmn2r14	UTSW	5	109,363,776 (GRCm39)	missense	probably damaging	1.00
R3120:Vmn2r14	UTSW	5	109,372,431 (GRCm39)	missense	probably null	0.00
R3762:Vmn2r14	UTSW	5	109,368,033 (GRCm39)	missense	probably benign	0.02
R3943:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R3944:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R4222:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4224:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4239:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4240:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4782:Vmn2r14	UTSW	5	109,369,370 (GRCm39)	missense	probably benign	0.01
R4832:Vmn2r14	UTSW	5	109,363,976 (GRCm39)	missense	probably damaging	1.00
R4884:Vmn2r14	UTSW	5	109,369,384 (GRCm39)	splice site	probably null
R4896:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5004:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5117:Vmn2r14	UTSW	5	109,363,961 (GRCm39)	missense	probably benign	0.16
R5285:Vmn2r14	UTSW	5	109,365,442 (GRCm39)	missense	probably damaging	0.98
R5413:Vmn2r14	UTSW	5	109,369,154 (GRCm39)	missense	probably benign	0.29
R5569:Vmn2r14	UTSW	5	109,368,261 (GRCm39)	missense	probably benign	0.44
R5701:Vmn2r14	UTSW	5	109,367,816 (GRCm39)	missense	probably damaging	1.00
R5726:Vmn2r14	UTSW	5	109,365,486 (GRCm39)	missense	possibly damaging	0.95
R5763:Vmn2r14	UTSW	5	109,363,724 (GRCm39)	missense	possibly damaging	0.49
R5872:Vmn2r14	UTSW	5	109,369,222 (GRCm39)	missense	probably benign
R5985:Vmn2r14	UTSW	5	109,368,082 (GRCm39)	missense	possibly damaging	0.89
R6268:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	possibly damaging	0.87
R6273:Vmn2r14	UTSW	5	109,369,133 (GRCm39)	missense	probably benign	0.44
R6409:Vmn2r14	UTSW	5	109,364,096 (GRCm39)	missense	probably benign	0.09
R6944:Vmn2r14	UTSW	5	109,364,140 (GRCm39)	missense	probably benign	0.06
R6944:Vmn2r14	UTSW	5	109,363,925 (GRCm39)	missense	probably benign	0.22
R7608:Vmn2r14	UTSW	5	109,369,276 (GRCm39)	missense	probably benign	0.03
R7740:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7768:Vmn2r14	UTSW	5	109,368,086 (GRCm39)	missense	probably benign	0.01
R7804:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7872:Vmn2r14	UTSW	5	109,369,219 (GRCm39)	missense	probably benign	0.02
R7993:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R8006:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8007:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8187:Vmn2r14	UTSW	5	109,368,420 (GRCm39)	missense	probably benign	0.03
R8369:Vmn2r14	UTSW	5	109,369,342 (GRCm39)	missense	probably damaging	1.00
R8463:Vmn2r14	UTSW	5	109,369,340 (GRCm39)	missense	probably benign	0.30
R8968:Vmn2r14	UTSW	5	109,365,533 (GRCm39)	missense	probably benign	0.01
R9008:Vmn2r14	UTSW	5	109,367,893 (GRCm39)	missense	probably benign	0.00
R9030:Vmn2r14	UTSW	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
R9039:Vmn2r14	UTSW	5	109,367,902 (GRCm39)	nonsense	probably null
R9150:Vmn2r14	UTSW	5	109,367,783 (GRCm39)	missense	probably damaging	1.00
R9164:Vmn2r14	UTSW	5	109,364,087 (GRCm39)	missense	probably damaging	1.00
R9216:Vmn2r14	UTSW	5	109,369,112 (GRCm39)	missense	probably benign	0.01
R9225:Vmn2r14	UTSW	5	109,369,288 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r14	UTSW	5	109,368,176 (GRCm39)	missense	possibly damaging	0.89
R9342:Vmn2r14	UTSW	5	109,368,428 (GRCm39)	missense	probably damaging	1.00
R9472:Vmn2r14	UTSW	5	109,367,962 (GRCm39)	missense	probably benign	0.00
R9678:Vmn2r14	UTSW	5	109,364,041 (GRCm39)	missense	probably damaging	1.00
R9774:Vmn2r14	UTSW	5	109,369,126 (GRCm39)	missense	probably benign	0.07
Z1177:Vmn2r14	UTSW	5	109,367,741 (GRCm39)	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- AAGTTAGGTGCCCCTGATGC -3'
(R):5'- ACAGTGTGTGAGGTGTCCAG -3'

Sequencing Primer
(F):5'- ATCTTTCCCCCTGGAGAAGTGAG -3'
(R):5'- GTGTCCAGATGATAAGTATGCCAAC -3'

Posted On

2015-03-18