Mutagenetix > Incidental Mutation

Incidental Mutation 'R4398:Vmn1r25'

325582

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r25

Ensembl Gene

ENSMUSG00000115668

Gene Name

vomeronasal 1 receptor 25

Synonyms

V1rc8

MMRRC Submission

041130-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.107) question?

Stock #

R4398 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

57955379-57956287 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 57955812 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 159 (V159D)

Ref Sequence

ENSEMBL: ENSMUSP00000154074 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176572] [ENSMUST00000228585]

AlphaFold

H3BLP0

Predicted Effect

probably damaging
Transcript: ENSMUST00000176572
AA Change: V159D

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000135860
Gene: ENSMUSG00000115668
AA Change: V159D

Domain	Start	End	E-Value	Type
Pfam:V1R	29	293	5.4e-52	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000228585
AA Change: V159D

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.7%

Validation Efficiency

96% (53/55)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts20	C	A	15: 94,231,576 (GRCm39)	R871L	possibly damaging	Het
Adcy5	G	A	16: 35,089,363 (GRCm39)	C520Y	probably damaging	Het
AI661453	G	A	17: 47,779,042 (GRCm39)		probably benign	Het
Bptf	T	C	11: 107,001,670 (GRCm39)	K481E	probably damaging	Het
Cnot10	T	A	9: 114,460,949 (GRCm39)	K74*	probably null	Het
Ctc1	C	T	11: 68,913,697 (GRCm39)	P200S	probably damaging	Het
Dact1	T	C	12: 71,363,959 (GRCm39)	Y210H	probably damaging	Het
Dbn1	T	C	13: 55,623,194 (GRCm39)	T430A	probably benign	Het
Dmd	A	C	X: 82,765,624 (GRCm39)	T657P	probably benign	Het
Efnb2	C	T	8: 8,670,832 (GRCm39)	R256H	possibly damaging	Het
Eif4a1	T	C	11: 69,560,070 (GRCm39)	I116M	possibly damaging	Het
F730035P03Rik	A	T	7: 99,429,475 (GRCm39)		noncoding transcript	Het
Fbn1	C	T	2: 125,239,701 (GRCm39)	V329I	probably benign	Het
Gpr20	G	A	15: 73,568,125 (GRCm39)	T88I	probably benign	Het
Herc1	T	G	9: 66,386,735 (GRCm39)	V3783G	probably benign	Het
Khdc1a	A	G	1: 21,420,617 (GRCm39)	D79G	possibly damaging	Het
Klk1b16	A	T	7: 43,790,851 (GRCm39)	I218F	probably damaging	Het
Malrd1	C	T	2: 16,155,594 (GRCm39)	T2001I	unknown	Het
Mia3	A	G	1: 183,111,733 (GRCm39)	S556P	probably damaging	Het
Myo3a	T	A	2: 22,467,854 (GRCm39)	D369E	probably benign	Het
Nelfa	T	C	5: 34,058,623 (GRCm39)	D279G	possibly damaging	Het
Ntrk3	A	T	7: 77,900,517 (GRCm39)	C607*	probably null	Het
Or13a20	T	C	7: 140,232,741 (GRCm39)	V283A	possibly damaging	Het
Pclo	A	T	5: 14,825,380 (GRCm39)	Q1371L	probably damaging	Het
Pdzd2	A	T	15: 12,376,061 (GRCm39)	V1358E	probably benign	Het
Pgr	T	C	9: 8,903,750 (GRCm39)		probably null	Het
Prag1	A	G	8: 36,570,809 (GRCm39)	D464G	probably damaging	Het
Prickle4	A	G	17: 48,001,456 (GRCm39)		probably benign	Het
Prim2	A	G	1: 33,551,192 (GRCm39)	Y309H	probably damaging	Het
Prkaa1	A	G	15: 5,206,642 (GRCm39)	Q464R	possibly damaging	Het
Rab36	G	A	10: 74,880,328 (GRCm39)	V63I	probably damaging	Het
Rint1	T	A	5: 23,999,445 (GRCm39)	I78K	possibly damaging	Het
Rnf130	T	A	11: 49,962,205 (GRCm39)	F217Y	probably benign	Het
Smad7	T	C	18: 75,527,234 (GRCm39)	V360A	probably damaging	Het
Sp110	A	C	1: 85,505,050 (GRCm39)	F434C	probably benign	Het
Stag1	A	G	9: 100,838,659 (GRCm39)		probably benign	Het
Tlr12	T	A	4: 128,509,988 (GRCm39)	D754V	probably benign	Het
Tmf1	G	A	6: 97,155,857 (GRCm39)	P43L	probably damaging	Het
Togaram1	A	G	12: 65,027,630 (GRCm39)	N873S	probably benign	Het
Tsn	C	T	1: 118,238,799 (GRCm39)		probably benign	Het
Ubn1	A	G	16: 4,882,289 (GRCm39)	K250R	probably damaging	Het
Vmn2r89	T	C	14: 51,689,551 (GRCm39)	L18P	probably damaging	Het
Vps8	T	G	16: 21,323,216 (GRCm39)	N689K	probably damaging	Het
Ythdc1	T	A	5: 86,963,513 (GRCm39)	D30E	possibly damaging	Het
Ythdc1	G	T	5: 86,983,679 (GRCm39)		probably benign	Het
Zfp407	G	A	18: 84,580,856 (GRCm39)	Q86*	probably null	Het
Zfp521	C	T	18: 13,979,601 (GRCm39)	E271K	probably benign	Het

Other mutations in Vmn1r25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01867:Vmn1r25	APN	6	57,956,196 (GRCm39)	missense	probably damaging	0.99
R0299:Vmn1r25	UTSW	6	57,955,494 (GRCm39)	missense	probably damaging	1.00
R0401:Vmn1r25	UTSW	6	57,955,696 (GRCm39)	missense	probably benign	0.01
R0499:Vmn1r25	UTSW	6	57,955,494 (GRCm39)	missense	probably damaging	1.00
R1294:Vmn1r25	UTSW	6	57,955,464 (GRCm39)	missense	possibly damaging	0.55
R1562:Vmn1r25	UTSW	6	57,955,786 (GRCm39)	missense	probably benign	0.03
R1661:Vmn1r25	UTSW	6	57,955,446 (GRCm39)	missense	probably damaging	1.00
R1665:Vmn1r25	UTSW	6	57,955,446 (GRCm39)	missense	probably damaging	1.00
R1879:Vmn1r25	UTSW	6	57,955,912 (GRCm39)	missense	possibly damaging	0.50
R2221:Vmn1r25	UTSW	6	57,956,223 (GRCm39)	missense	probably damaging	1.00
R2223:Vmn1r25	UTSW	6	57,956,223 (GRCm39)	missense	probably damaging	1.00
R2374:Vmn1r25	UTSW	6	57,955,543 (GRCm39)	missense	probably benign	0.10
R4073:Vmn1r25	UTSW	6	57,955,572 (GRCm39)	missense	possibly damaging	0.94
R4590:Vmn1r25	UTSW	6	57,955,480 (GRCm39)	missense	probably benign	0.02
R4779:Vmn1r25	UTSW	6	57,956,011 (GRCm39)	missense	probably damaging	0.98
R5397:Vmn1r25	UTSW	6	57,956,060 (GRCm39)	nonsense	probably null
R6113:Vmn1r25	UTSW	6	57,955,557 (GRCm39)	missense	probably benign	0.00
R6858:Vmn1r25	UTSW	6	57,955,996 (GRCm39)	missense	probably benign	0.22
R7407:Vmn1r25	UTSW	6	57,956,044 (GRCm39)	missense	possibly damaging	0.76
R7748:Vmn1r25	UTSW	6	57,955,549 (GRCm39)	missense	probably damaging	1.00
R8001:Vmn1r25	UTSW	6	57,956,065 (GRCm39)	nonsense	probably null
R8472:Vmn1r25	UTSW	6	57,955,531 (GRCm39)	missense	possibly damaging	0.87
R9235:Vmn1r25	UTSW	6	57,955,488 (GRCm39)	missense	probably damaging	0.96
R9651:Vmn1r25	UTSW	6	57,956,306 (GRCm39)	unclassified	probably benign

Predicted Primers

PCR Primer
(F):5'- CCATAAGTAGTAGGATGGTCTGG -3'
(R):5'- AGGCTGTCACAATCAGTCCC -3'

Sequencing Primer
(F):5'- TTTCTCAGGGGACACTCTCAGG -3'
(R):5'- AATCAGTCCCCATACCTCATTGTTGG -3'

Posted On

2015-07-06