Mutagenetix > Incidental Mutation

Incidental Mutation 'R5148:Vmn2r3'

395189

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r3

Ensembl Gene

ENSMUSG00000091572

Gene Name

vomeronasal 2, receptor 3

Synonyms

EG637004

MMRRC Submission

043262-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5148 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64166225-64197130 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 64186247 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Glutamine at position 146 (P146Q)

Ref Sequence

ENSEMBL: ENSMUSP00000134891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170244] [ENSMUST00000176328]

AlphaFold

H3BJ88

Predicted Effect

probably damaging
Transcript: ENSMUST00000170244
AA Change: P118Q

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000126165
Gene: ENSMUSG00000091572
AA Change: P118Q

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	64	479	4e-64	PFAM
Pfam:NCD3G	521	574	1.1e-17	PFAM
Pfam:7tm_3	605	842	2.9e-75	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000176328
AA Change: P146Q

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000134891
Gene: ENSMUSG00000091572
AA Change: P146Q

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	92	507	9.5e-66	PFAM
Pfam:NCD3G	549	602	8.8e-17	PFAM
Pfam:7tm_3	635	869	8.5e-48	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.2%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ank2	A	T	3: 126,819,285 (GRCm39)		probably null	Het
Atf7	A	G	15: 102,455,608 (GRCm39)	M252T	probably benign	Het
Cacna1h	C	A	17: 25,606,519 (GRCm39)	D1027Y	probably damaging	Het
Cngb1	A	G	8: 95,992,611 (GRCm39)	V667A	probably benign	Het
Cntf	T	C	19: 12,741,368 (GRCm39)	E164G	probably damaging	Het
Dbt	T	C	3: 116,321,893 (GRCm39)		probably benign	Het
Egfem1	G	A	3: 29,511,972 (GRCm39)		probably benign	Het
Gm12790	C	T	4: 101,825,268 (GRCm39)	V49I	possibly damaging	Het
Gm5444	C	T	13: 4,884,314 (GRCm39)		noncoding transcript	Het
Gpcpd1	A	T	2: 132,376,110 (GRCm39)	Y574*	probably null	Het
Gss	G	T	2: 155,415,029 (GRCm39)	N225K	possibly damaging	Het
Il17rc	G	T	6: 113,459,958 (GRCm39)	A635S	probably benign	Het
Lhfpl5	T	A	17: 28,798,942 (GRCm39)	D150E	probably damaging	Het
Lin9	T	A	1: 180,496,763 (GRCm39)	L351I	probably benign	Het
Lrrc40	T	G	3: 157,760,206 (GRCm39)		probably null	Het
Ly6f	A	G	15: 75,143,646 (GRCm39)	T118A	probably benign	Het
Malrd1	A	G	2: 16,147,037 (GRCm39)	N1960D	unknown	Het
Map3k14	T	C	11: 103,130,158 (GRCm39)	H253R	probably benign	Het
Morc2a	T	C	11: 3,639,084 (GRCm39)	L1025P	probably damaging	Het
Nlrc5	G	T	8: 95,203,321 (GRCm39)	G474W	probably damaging	Het
Olr1	G	T	6: 129,470,572 (GRCm39)	D198E	probably benign	Het
Or5b123	C	A	19: 13,596,874 (GRCm39)	S116*	probably null	Het
Or5d14	G	A	2: 87,880,737 (GRCm39)	T77I	probably benign	Het
Or8c16	T	C	9: 38,130,317 (GRCm39)	I66T	probably benign	Het
Pafah1b1	A	T	11: 74,575,278 (GRCm39)	S209T	probably damaging	Het
Phf14	A	T	6: 11,961,641 (GRCm39)	Y426F	possibly damaging	Het
Phldb1	A	G	9: 44,615,455 (GRCm39)	V855A	probably benign	Het
Pira2	T	G	7: 3,847,592 (GRCm39)	R32S	possibly damaging	Het
Pnldc1	T	C	17: 13,111,676 (GRCm39)	I344V	probably benign	Het
Prss29	T	C	17: 25,539,881 (GRCm39)	V93A	probably benign	Het
Ptpn13	C	T	5: 103,640,098 (GRCm39)	L186F	probably damaging	Het
Ralgps1	A	C	2: 33,048,999 (GRCm39)	C303W	probably damaging	Het
Sdr42e1	T	A	8: 118,390,342 (GRCm39)	N100Y	probably damaging	Het
Serpina11	A	T	12: 103,952,503 (GRCm39)	L96Q	probably damaging	Het
Slc12a8	G	A	16: 33,445,288 (GRCm39)	R448H	probably benign	Het
Snrpd1	T	A	18: 10,626,892 (GRCm39)	V53E	probably benign	Het
Ssbp1	T	C	6: 40,454,883 (GRCm39)	V114A	possibly damaging	Het
T	A	G	17: 8,655,037 (GRCm39)	E47G	probably damaging	Het
Tmem156	T	C	5: 65,231,111 (GRCm39)	K189R	probably benign	Het
Trim47	G	T	11: 115,998,678 (GRCm39)	Q314K	possibly damaging	Het
Vmn2r6	A	C	3: 64,464,015 (GRCm39)	V273G	probably damaging	Het
Wrnip1	G	T	13: 32,990,839 (GRCm39)	R366L	probably damaging	Het
Zfp981	A	T	4: 146,621,357 (GRCm39)	H94L	possibly damaging	Het

Other mutations in Vmn2r3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r3	APN	3	64,167,304 (GRCm39)	missense	probably damaging	1.00
IGL01468:Vmn2r3	APN	3	64,182,382 (GRCm39)	missense	possibly damaging	0.57
IGL02032:Vmn2r3	APN	3	64,182,476 (GRCm39)	missense	possibly damaging	0.95
IGL02405:Vmn2r3	APN	3	64,178,620 (GRCm39)	splice site	probably benign
IGL02640:Vmn2r3	APN	3	64,194,816 (GRCm39)	missense	probably benign
IGL02719:Vmn2r3	APN	3	64,183,031 (GRCm39)	missense	probably damaging	1.00
IGL02746:Vmn2r3	APN	3	64,167,239 (GRCm39)	missense	possibly damaging	0.74
IGL02952:Vmn2r3	APN	3	64,186,256 (GRCm39)	missense	probably damaging	1.00
IGL03390:Vmn2r3	APN	3	64,182,767 (GRCm39)	missense	possibly damaging	0.55
G1citation:Vmn2r3	UTSW	3	64,194,876 (GRCm39)	missense	probably benign	0.30
R0023:Vmn2r3	UTSW	3	64,182,787 (GRCm39)	missense	probably damaging	0.99
R0433:Vmn2r3	UTSW	3	64,183,054 (GRCm39)	missense	possibly damaging	0.51
R0647:Vmn2r3	UTSW	3	64,183,046 (GRCm39)	missense	probably damaging	1.00
R1071:Vmn2r3	UTSW	3	64,182,697 (GRCm39)	missense	possibly damaging	0.79
R1536:Vmn2r3	UTSW	3	64,182,538 (GRCm39)	missense	probably damaging	1.00
R1806:Vmn2r3	UTSW	3	64,194,810 (GRCm39)	missense	possibly damaging	0.73
R1806:Vmn2r3	UTSW	3	64,182,893 (GRCm39)	missense	probably benign	0.03
R1852:Vmn2r3	UTSW	3	64,166,815 (GRCm39)	missense	probably damaging	1.00
R1868:Vmn2r3	UTSW	3	64,166,537 (GRCm39)	missense	probably damaging	1.00
R2072:Vmn2r3	UTSW	3	64,182,493 (GRCm39)	missense	possibly damaging	0.87
R2240:Vmn2r3	UTSW	3	64,166,483 (GRCm39)	missense	probably benign	0.44
R2446:Vmn2r3	UTSW	3	64,182,733 (GRCm39)	missense	probably damaging	0.98
R4133:Vmn2r3	UTSW	3	64,183,138 (GRCm39)	missense	probably damaging	0.99
R4159:Vmn2r3	UTSW	3	64,194,850 (GRCm39)	nonsense	probably null
R4494:Vmn2r3	UTSW	3	64,182,692 (GRCm39)	missense	probably damaging	1.00
R4860:Vmn2r3	UTSW	3	64,183,022 (GRCm39)	missense	probably benign	0.00
R4895:Vmn2r3	UTSW	3	64,167,182 (GRCm39)	missense	probably benign	0.00
R4912:Vmn2r3	UTSW	3	64,166,618 (GRCm39)	missense	probably damaging	1.00
R5018:Vmn2r3	UTSW	3	64,178,774 (GRCm39)	missense	probably benign
R5033:Vmn2r3	UTSW	3	64,167,220 (GRCm39)	missense	probably benign	0.09
R5126:Vmn2r3	UTSW	3	64,166,740 (GRCm39)	missense	probably damaging	1.00
R5414:Vmn2r3	UTSW	3	64,166,978 (GRCm39)	nonsense	probably null
R5785:Vmn2r3	UTSW	3	64,166,444 (GRCm39)	missense	possibly damaging	0.89
R5905:Vmn2r3	UTSW	3	64,182,698 (GRCm39)	missense	probably benign	0.19
R5992:Vmn2r3	UTSW	3	64,167,068 (GRCm39)	missense	probably damaging	1.00
R6028:Vmn2r3	UTSW	3	64,182,698 (GRCm39)	missense	probably benign	0.19
R6331:Vmn2r3	UTSW	3	64,186,182 (GRCm39)	missense	probably damaging	1.00
R6378:Vmn2r3	UTSW	3	64,182,517 (GRCm39)	missense	probably damaging	1.00
R6775:Vmn2r3	UTSW	3	64,183,039 (GRCm39)	missense	possibly damaging	0.88
R6822:Vmn2r3	UTSW	3	64,194,876 (GRCm39)	missense	probably benign	0.30
R6826:Vmn2r3	UTSW	3	64,182,327 (GRCm39)	nonsense	probably null
R6886:Vmn2r3	UTSW	3	64,166,927 (GRCm39)	missense	probably damaging	1.00
R6971:Vmn2r3	UTSW	3	64,166,668 (GRCm39)	missense	probably damaging	0.99
R7154:Vmn2r3	UTSW	3	64,194,732 (GRCm39)	missense	probably benign	0.02
R7192:Vmn2r3	UTSW	3	64,167,364 (GRCm39)	missense	probably benign	0.24
R7282:Vmn2r3	UTSW	3	64,168,825 (GRCm39)	missense	possibly damaging	0.90
R7472:Vmn2r3	UTSW	3	64,182,953 (GRCm39)	missense	probably benign	0.00
R7563:Vmn2r3	UTSW	3	64,182,770 (GRCm39)	missense	possibly damaging	0.60
R7726:Vmn2r3	UTSW	3	64,182,939 (GRCm39)	nonsense	probably null
R7966:Vmn2r3	UTSW	3	64,186,235 (GRCm39)	missense	probably damaging	0.99
R8025:Vmn2r3	UTSW	3	64,182,871 (GRCm39)	missense	possibly damaging	0.91
R8050:Vmn2r3	UTSW	3	64,178,714 (GRCm39)	missense	probably damaging	0.99
R8300:Vmn2r3	UTSW	3	64,182,347 (GRCm39)	missense	probably benign	0.00
R8402:Vmn2r3	UTSW	3	64,178,617 (GRCm39)	splice site	probably benign
R8486:Vmn2r3	UTSW	3	64,186,370 (GRCm39)	missense	probably damaging	1.00
R8523:Vmn2r3	UTSW	3	64,182,311 (GRCm39)	missense	probably benign	0.03
R8678:Vmn2r3	UTSW	3	64,166,896 (GRCm39)	missense	possibly damaging	0.76
R8885:Vmn2r3	UTSW	3	64,182,383 (GRCm39)	missense	probably benign	0.00
R8886:Vmn2r3	UTSW	3	64,194,892 (GRCm39)	missense	possibly damaging	0.47
R8905:Vmn2r3	UTSW	3	64,166,695 (GRCm39)	missense	probably damaging	0.99
R8937:Vmn2r3	UTSW	3	64,166,673 (GRCm39)	missense	probably damaging	1.00
R8955:Vmn2r3	UTSW	3	64,168,803 (GRCm39)	missense	possibly damaging	0.56
R9172:Vmn2r3	UTSW	3	64,186,403 (GRCm39)	missense	possibly damaging	0.79
R9485:Vmn2r3	UTSW	3	64,183,046 (GRCm39)	missense	probably damaging	1.00
R9575:Vmn2r3	UTSW	3	64,178,735 (GRCm39)	missense	probably benign	0.01
R9618:Vmn2r3	UTSW	3	64,178,724 (GRCm39)	missense	probably damaging	0.98
X0022:Vmn2r3	UTSW	3	64,182,389 (GRCm39)	missense	probably damaging	1.00
X0022:Vmn2r3	UTSW	3	64,178,669 (GRCm39)	missense	possibly damaging	0.62

Predicted Primers

PCR Primer
(F):5'- TAGCTTAGTGCAGCATCTCTGG -3'
(R):5'- GTTTCCGCTGGATGAAAACC -3'

Sequencing Primer
(F):5'- GTGCAACAGCCCTAAATGTCTTGG -3'
(R):5'- TTCCGCTGGATGAAAACCATGATC -3'

Posted On

2016-06-21