Mutagenetix > Incidental Mutation

Incidental Mutation 'R5186:Or8g22'

397888

Institutional Source

Beutler Lab

Gene Symbol

Or8g22

Ensembl Gene

ENSMUSG00000095194

Gene Name

olfactory receptor family 8 subfamily G member 22, pseudogene 1

Synonyms

Olfr936, MOR171-37, EG628171, GA_x6K02T2PVTD-32743332-32742397

MMRRC Submission

042765-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R5186 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

38957910-38958845 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 38958265 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 194 (C194*)

Ref Sequence

ENSEMBL: ENSMUSP00000148891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000178303] [ENSMUST00000216912]

AlphaFold

J3KMV2

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000104850

Predicted Effect

probably damaging
Transcript: ENSMUST00000178303
AA Change: V150E

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000137209
Gene: ENSMUSG00000095194
AA Change: V150E

Domain	Start	End	E-Value	Type
Pfam:7tm_1	1	246	6e-21	PFAM
Pfam:7tm_4	1	264	6.9e-49	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000216912
AA Change: C194*

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Feb 2010]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	T	C	11: 109,982,425 (GRCm39)	I6V	probably null	Het
Aox1	A	G	1: 58,107,529 (GRCm39)	D601G	probably damaging	Het
Asic1	GCACC	GCACCACC	15: 99,596,684 (GRCm39)		probably benign	Het
Cacna1g	T	G	11: 94,333,674 (GRCm39)	N931T	probably damaging	Het
Ccdc14	T	C	16: 34,541,955 (GRCm39)	F511L	probably damaging	Het
Cd177	T	A	7: 24,444,348 (GRCm39)	E710V	probably benign	Het
Cep112	T	C	11: 108,643,386 (GRCm39)	C49R	probably benign	Het
Clip2	G	A	5: 134,551,645 (GRCm39)	T159M	possibly damaging	Het
Dnah2	T	C	11: 69,326,710 (GRCm39)	N3575S	probably damaging	Het
Dnah6	T	A	6: 73,044,410 (GRCm39)	I3234F	probably damaging	Het
Eci3	G	T	13: 35,130,961 (GRCm39)	A302E	possibly damaging	Het
Fam204a	T	C	19: 60,188,421 (GRCm39)	K214E	probably damaging	Het
Fam78a	T	C	2: 31,972,666 (GRCm39)	T85A	possibly damaging	Het
Flnb	T	C	14: 7,909,748 (GRCm38)	Y1401H	probably damaging	Het
Foxl2	A	T	9: 98,838,108 (GRCm39)	D132V	probably damaging	Het
Frs2	C	A	10: 116,914,747 (GRCm39)	W57C	probably damaging	Het
Gm26558	G	T	2: 70,491,761 (GRCm39)		probably benign	Het
Gpr139	A	G	7: 118,744,063 (GRCm39)	V174A	probably benign	Het
Grik5	T	C	7: 24,715,244 (GRCm39)	T676A	probably damaging	Het
H60c	T	C	10: 3,209,273 (GRCm39)		probably null	Het
Hspa1l	A	G	17: 35,197,445 (GRCm39)	K495E	probably damaging	Het
Irgm1	C	T	11: 48,757,044 (GRCm39)	V256I	probably benign	Het
Kat7	T	A	11: 95,177,242 (GRCm39)	T293S	probably benign	Het
Lipg	C	T	18: 75,094,009 (GRCm39)	V13I	probably benign	Het
Lrrn1	A	T	6: 107,546,185 (GRCm39)	Y661F	probably damaging	Het
Mllt3	A	G	4: 87,759,232 (GRCm39)	V272A	probably benign	Het
Mx1	G	A	16: 97,256,694 (GRCm39)	R162C	probably benign	Het
Myo18b	T	A	5: 113,019,336 (GRCm39)	D647V	probably damaging	Het
Naf1	G	A	8: 67,332,298 (GRCm39)	V329I	probably benign	Het
Or4k40	A	T	2: 111,251,119 (GRCm39)	M59K	probably damaging	Het
Or52u1	A	T	7: 104,237,418 (GRCm39)	I153F	probably damaging	Het
P2rx5	G	A	11: 73,062,616 (GRCm39)	V442M	possibly damaging	Het
Pcdhb9	A	G	18: 37,534,285 (GRCm39)	E93G	probably damaging	Het
Pcdhga4	A	T	18: 37,820,479 (GRCm39)	N676I	probably benign	Het
Pgm5	A	G	19: 24,797,492 (GRCm39)	M230T	probably damaging	Het
Pik3c2g	T	C	6: 139,599,016 (GRCm39)	V44A	probably damaging	Het
Pp2d1	T	C	17: 53,815,168 (GRCm39)	M519V	probably benign	Het
Ppp1r10	A	G	17: 36,239,403 (GRCm39)	E404G	probably damaging	Het
Prpf8	A	T	11: 75,380,609 (GRCm39)	E104V	possibly damaging	Het
Ptpra	T	C	2: 30,328,367 (GRCm39)		probably null	Het
Pygl	A	C	12: 70,248,118 (GRCm39)	N248K	probably damaging	Het
Rbm8a	A	G	3: 96,538,248 (GRCm39)	D102G	probably damaging	Het
Sema3d	A	G	5: 12,634,875 (GRCm39)	D647G	probably benign	Het
Serpinb11	A	T	1: 107,307,484 (GRCm39)	D305V	probably damaging	Het
Slc12a8	T	C	16: 33,437,578 (GRCm39)	I337T	probably damaging	Het
Slc29a2	G	A	19: 5,078,995 (GRCm39)	R286Q	probably benign	Het
Slc2a3	T	A	6: 122,712,542 (GRCm39)	D234V	probably damaging	Het
Slco4a1	T	C	2: 180,114,901 (GRCm39)	V608A	probably damaging	Het
Spata31d1e	A	T	13: 59,891,553 (GRCm39)	L89H	probably damaging	Het
Srrm2	C	T	17: 24,035,561 (GRCm39)	T831I	probably benign	Het
St18	T	A	1: 6,872,541 (GRCm39)		probably null	Het
Tesk2	G	C	4: 116,599,093 (GRCm39)	G67A	probably damaging	Het
Tlr1	A	T	5: 65,082,564 (GRCm39)	L671H	probably damaging	Het
Tmem63b	A	T	17: 45,972,403 (GRCm39)	Y735N	possibly damaging	Het
Tmprss11a	C	T	5: 86,567,938 (GRCm39)	C263Y	probably damaging	Het
Trio	A	T	15: 27,898,077 (GRCm39)	V345E	probably damaging	Het
Tut7	A	G	13: 59,964,470 (GRCm39)		probably null	Het
Ubr5	A	G	15: 37,998,160 (GRCm39)	S1674P	probably damaging	Het
Uchl3	T	A	14: 101,933,353 (GRCm39)	C209S	probably damaging	Het
Uhmk1	T	C	1: 170,038,736 (GRCm39)	N206S	probably damaging	Het
Uhrf1	C	T	17: 56,625,340 (GRCm39)	R588W	probably damaging	Het
Usp28	T	C	9: 48,921,550 (GRCm39)	V256A	probably damaging	Het
Utrn	C	A	10: 12,604,521 (GRCm39)	L552F	probably damaging	Het
Vmn1r55	A	T	7: 5,149,985 (GRCm39)	M146K	probably damaging	Het
Vmn1r57	A	C	7: 5,224,107 (GRCm39)	I211L	probably benign	Het
Zar1	C	T	5: 72,734,742 (GRCm39)	C316Y	probably damaging	Het
Zc3h11a	A	T	1: 133,549,412 (GRCm39)	S750T	probably damaging	Het
Zfp366	G	A	13: 99,382,676 (GRCm39)	C613Y	probably benign	Het
Zfp37	A	T	4: 62,109,493 (GRCm39)	C524S	probably damaging	Het
Zfp516	T	C	18: 82,975,218 (GRCm39)	V472A	probably benign	Het
Zhx1	A	T	15: 57,915,819 (GRCm39)	M809K	probably damaging	Het
Zic1	T	C	9: 91,246,424 (GRCm39)	Y216C	probably damaging	Het

Other mutations in Or8g22

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01928:Or8g22	APN	9	38,958,709 (GRCm39)	missense	unknown
R0086:Or8g22	UTSW	9	38,958,191 (GRCm39)	missense	probably benign	0.00
R0561:Or8g22	UTSW	9	38,958,669 (GRCm39)	missense	probably damaging	0.99
R0650:Or8g22	UTSW	9	38,957,996 (GRCm39)	missense	probably benign	0.01
R1221:Or8g22	UTSW	9	38,958,483 (GRCm39)	missense	probably damaging	1.00
R1384:Or8g22	UTSW	9	38,958,200 (GRCm39)	missense	possibly damaging	0.70
R1680:Or8g22	UTSW	9	38,958,296 (GRCm39)	missense	probably benign	0.43
R1733:Or8g22	UTSW	9	38,958,678 (GRCm39)	missense	unknown
R3767:Or8g22	UTSW	9	38,958,707 (GRCm39)	missense	unknown
R4786:Or8g22	UTSW	9	38,958,783 (GRCm39)	nonsense	probably null
R4944:Or8g22	UTSW	9	38,958,158 (GRCm39)	missense	probably damaging	1.00
R5403:Or8g22	UTSW	9	38,957,999 (GRCm39)	missense	probably damaging	1.00
R6037:Or8g22	UTSW	9	38,958,403 (GRCm39)	missense	probably damaging	1.00
R6037:Or8g22	UTSW	9	38,958,403 (GRCm39)	missense	probably damaging	1.00
R6156:Or8g22	UTSW	9	38,958,671 (GRCm39)	missense	possibly damaging	0.90
R6217:Or8g22	UTSW	9	38,958,039 (GRCm39)	makesense	probably null
R6711:Or8g22	UTSW	9	38,958,162 (GRCm39)	makesense	probably null
R6919:Or8g22	UTSW	9	38,958,827 (GRCm39)	utr 5 prime	probably benign
R7022:Or8g22	UTSW	9	38,958,379 (GRCm39)	nonsense	probably null
R7275:Or8g22	UTSW	9	38,958,815 (GRCm39)	utr 5 prime	probably benign
R7290:Or8g22	UTSW	9	38,958,694 (GRCm39)	missense	unknown
R7644:Or8g22	UTSW	9	38,958,638 (GRCm39)	missense	probably damaging	1.00
R8906:Or8g22	UTSW	9	38,958,077 (GRCm39)	missense	possibly damaging	0.87
R9099:Or8g22	UTSW	9	38,958,026 (GRCm39)	missense	probably benign	0.00
Z1176:Or8g22	UTSW	9	38,958,215 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GGTTTCAGATACATAAATGCAGCAG -3'
(R):5'- ATGTTGCCATCTGTAGCCCC -3'

Sequencing Primer
(F):5'- GATACATAAATGCAGCAGAACCG -3'
(R):5'- GCTTTACAGTAGCATCATGTCTCAAC -3'

Posted On

2016-07-06