Mutagenetix > Incidental Mutations

Incidental Mutation 'R5921:Oas3'

461573

Institutional Source

Beutler Lab

Gene Symbol

Oas3

Ensembl Gene

ENSMUSG00000032661

Gene Name

2'-5' oligoadenylate synthetase 3

Synonyms

Oasl10, 2'-5' oligoadenylate synthetase-like 10

MMRRC Submission

044118-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R5921 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

120753098-120777661 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 120769981 bp

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 298 (D298G)

Ref Sequence

ENSEMBL: ENSMUSP00000035588 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044833]

Predicted Effect

probably damaging
Transcript: ENSMUST00000044833
AA Change: D298G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000035588
Gene: ENSMUSG00000032661
AA Change: D298G

Domain	Start	End	E-Value	Type
Pfam:OAS1_C	159	341	6.3e-83	PFAM
Pfam:OAS1_C	610	795	3.1e-78	PFAM
Pfam:NTP_transf_2	831	920	4.5e-11	PFAM
Pfam:OAS1_C	954	1136	9e-85	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000166703

Meta Mutation Damage Score

0.7689

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.7%

Validation Efficiency

95% (70/74)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932443I19Rik	G	T	8: 13,734,840	A34S	probably damaging	Het
Ablim2	G	A	5: 35,812,211	V223M	probably damaging	Het
Adamts7	T	C	9: 90,188,694	S623P	probably benign	Het
Aqp7	G	T	4: 41,036,093	N48K	probably benign	Het
Asic4	A	G	1: 75,451,373	N181S	probably benign	Het
Blvra	A	T	2: 127,087,363		probably benign	Het
Bmf	C	A	2: 118,532,553		probably benign	Het
Bnc2	A	T	4: 84,293,055	I454N	possibly damaging	Het
Catsperg1	A	T	7: 29,190,523	L700H	possibly damaging	Het
Ccdc14	T	C	16: 34,706,391	V222A	probably damaging	Het
Clstn3	A	T	6: 124,431,580		probably benign	Het
Col15a1	A	T	4: 47,300,602	I1066F	probably damaging	Het
Dcdc2c	T	C	12: 28,524,775	E116G	possibly damaging	Het
Dopey1	G	A	9: 86,501,922	S310N	probably damaging	Het
Dync1h1	T	A	12: 110,618,368	V735E	probably damaging	Het
Eva1a	T	C	6: 82,092,159	Y156H	probably damaging	Het
Fbxw26	A	G	9: 109,746,018	I13T	probably damaging	Het
Fermt2	A	T	14: 45,464,746	L527Q	probably damaging	Het
Fxyd4	G	A	6: 117,936,138		probably benign	Het
Gal	A	G	19: 3,410,100	S124P	probably damaging	Het
Glmp	T	C	3: 88,325,976	S56P	probably benign	Het
Gm5600	T	C	7: 113,708,176		noncoding transcript	Het
Golga2	A	G	2: 32,297,755	N194S	probably benign	Het
Gon4l	T	C	3: 88,909,947		probably benign	Het
Gtf2ird2	T	A	5: 134,217,584	Y895N	probably damaging	Het
Hsd3b1	C	A	3: 98,857,899	M22I	probably benign	Het
Ipo13	A	C	4: 117,912,089	L169V	probably benign	Het
Kif13a	G	T	13: 46,825,300	T208K	probably damaging	Het
Klhl5	G	T	5: 65,162,956	A618S	probably damaging	Het
Lrig2	A	T	3: 104,462,754	L496*	probably null	Het
Macf1	A	G	4: 123,526,711	I250T	probably benign	Het
Man1a	A	G	10: 53,907,510	I632T	probably damaging	Het
Nav2	A	G	7: 49,304,576		probably benign	Het
Nek8	A	G	11: 78,173,059	M40T	probably damaging	Het
Ociad1	A	G	5: 73,310,382	D167G	probably benign	Het
Olfr115	A	T	17: 37,610,219	C177*	probably null	Het
Olfr12	T	A	1: 92,620,622	S239T	probably benign	Het
Olfr284	T	C	15: 98,340,429	T187A	probably benign	Het
Pafah2	G	T	4: 134,418,069	V255L	probably benign	Het
Pde10a	A	G	17: 8,930,537	Y407C	probably damaging	Het
Pirb	A	C	7: 3,716,694	Y484*	probably null	Het
Prl8a6	A	G	13: 27,437,188	S20P	probably damaging	Het
R3hdm4	A	T	10: 79,913,619	V52E	probably damaging	Het
Rab3ip	A	G	10: 116,939,247	Y69H	probably damaging	Het
Rxrg	T	C	1: 167,639,239	M330T	possibly damaging	Het
Sema4g	G	T	19: 44,998,704	G460V	probably benign	Het
Sidt1	T	C	16: 44,273,735		probably benign	Het
Slc12a2	T	A	18: 57,932,523	D943E	probably benign	Het
Slc12a4	T	C	8: 105,945,244		probably null	Het
Slc4a3	T	G	1: 75,557,444		probably null	Het
Slc4a8	C	T	15: 100,814,447		probably benign	Het
Srcap	T	A	7: 127,558,833		probably benign	Het
Stk39	A	G	2: 68,366,105	S327P	probably damaging	Het
Tbc1d5	G	A	17: 50,963,693	T170M	probably damaging	Het
Trim13	T	A	14: 61,605,089	F185Y	probably benign	Het
Ttc17	A	G	2: 94,378,848	V87A	probably damaging	Het
Ttn	A	T	2: 76,720,863	M31395K	possibly damaging	Het
Usp34	T	A	11: 23,464,686	D2876E	probably damaging	Het
Uvssa	T	C	5: 33,389,752	S221P	probably benign	Het
Vmn2r93	T	A	17: 18,325,768	L634Q	probably damaging	Het
Vmp1	C	T	11: 86,586,510	A355T	probably benign	Het
Xpo5	A	T	17: 46,221,421	M461L	probably benign	Het
Zfp759	T	A	13: 67,140,494	F703Y	probably damaging	Het

Other mutations in Oas3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00547:Oas3	APN	5	120777442	splice site	probably benign
IGL01095:Oas3	APN	5	120772889	missense	probably damaging	1.00
IGL01835:Oas3	APN	5	120766128	nonsense	probably null
IGL02006:Oas3	APN	5	120769235	missense	probably benign	0.00
IGL02811:Oas3	APN	5	120764322	missense	unknown
IGL03194:Oas3	APN	5	120758953	missense	probably damaging	1.00
R0066:Oas3	UTSW	5	120758875	missense	probably damaging	1.00
R0195:Oas3	UTSW	5	120756145	missense	probably damaging	1.00
R0196:Oas3	UTSW	5	120756145	missense	probably damaging	1.00
R0197:Oas3	UTSW	5	120756145	missense	probably damaging	1.00
R0445:Oas3	UTSW	5	120756145	missense	probably damaging	1.00
R0523:Oas3	UTSW	5	120766144	missense	unknown
R0592:Oas3	UTSW	5	120771149	missense	probably damaging	1.00
R0946:Oas3	UTSW	5	120769063	missense	unknown
R1354:Oas3	UTSW	5	120770000	missense	possibly damaging	0.94
R1642:Oas3	UTSW	5	120777574	missense	possibly damaging	0.90
R1681:Oas3	UTSW	5	120769908	missense	probably benign	0.22
R1844:Oas3	UTSW	5	120759980	missense	probably damaging	0.99
R1981:Oas3	UTSW	5	120761835	splice site	probably benign
R2443:Oas3	UTSW	5	120777488	missense	probably benign	0.35
R2902:Oas3	UTSW	5	120758917	missense	probably damaging	1.00
R3034:Oas3	UTSW	5	120771056	missense	probably damaging	1.00
R4565:Oas3	UTSW	5	120771039	missense	probably damaging	1.00
R4692:Oas3	UTSW	5	120769355	missense	probably benign	0.03
R4723:Oas3	UTSW	5	120766256	missense	unknown
R4812:Oas3	UTSW	5	120761147	unclassified	probably benign
R5288:Oas3	UTSW	5	120756990	missense	probably damaging	1.00
R5343:Oas3	UTSW	5	120756238	missense	possibly damaging	0.70
R5494:Oas3	UTSW	5	120761644	missense	unknown
R5688:Oas3	UTSW	5	120758802	missense	probably benign	0.31
R5894:Oas3	UTSW	5	120756954	missense	probably damaging	1.00
R6037:Oas3	UTSW	5	120769319	missense	probably benign	0.41
R6037:Oas3	UTSW	5	120769319	missense	probably benign	0.41
R6066:Oas3	UTSW	5	120772924	missense	probably damaging	0.97
R6104:Oas3	UTSW	5	120761693	missense	unknown
R6134:Oas3	UTSW	5	120769048	missense	unknown
R6255:Oas3	UTSW	5	120771230	missense	probably benign	0.04
R6257:Oas3	UTSW	5	120761135	unclassified	probably benign
R6776:Oas3	UTSW	5	120758874	missense	probably damaging	1.00
R8022:Oas3	UTSW	5	120756966	missense	possibly damaging	0.91
R8137:Oas3	UTSW	5	120777500	missense	probably benign	0.07
RF006:Oas3	UTSW	5	120774100	missense	probably damaging	1.00
X0024:Oas3	UTSW	5	120761728	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TCTAGGTGGACAGACATATCCAG -3'
(R):5'- TCCAGCAAAGTGGGTGATACC -3'

Sequencing Primer
(F):5'- TGGACAGACATATCCAGAGAGC -3'
(R):5'- AGTGGGTGATACCATCATTATGC -3'

Posted On

2017-02-28