Mutagenetix > Incidental Mutation

Incidental Mutation 'R7669:Or7e165'

592048

Institutional Source

Beutler Lab

Gene Symbol

Or7e165

Ensembl Gene

ENSMUSG00000058659

Gene Name

olfactory receptor family 7 subfamily E member 165

Synonyms

Olfr58, MOR146-7P, IG6, GA_x6K02T2PVTD-13523015-13523944, MOR146-3

MMRRC Submission

045741-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R7669 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19691579-19695360 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 19694839 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 137 (N137D)

Ref Sequence

ENSEMBL: ENSMUSP00000150309 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000212363] [ENSMUST00000215112]

AlphaFold

Q7TRF6

Predicted Effect

probably benign
Transcript: ENSMUST00000212363

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215112
AA Change: N137D

PolyPhen 2 Score 0.794 (Sensitivity: 0.85; Specificity: 0.93)

Meta Mutation Damage Score

0.2781

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

100% (52/52)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars2	C	T	17: 45,831,221 (GRCm39)	P930S	probably benign	Het
Ada	T	A	2: 163,570,111 (GRCm39)	K301*	probably null	Het
Alb	T	C	5: 90,611,850 (GRCm39)	L93P	possibly damaging	Het
Aldh1l1	G	A	6: 90,547,844 (GRCm39)	G435S	probably benign	Het
Alox12b	A	T	11: 69,060,167 (GRCm39)	I627F	probably benign	Het
Aloxe3	A	G	11: 69,025,946 (GRCm39)	I503V	probably benign	Het
Arhgap29	C	T	3: 121,786,461 (GRCm39)	A342V	probably damaging	Het
BC024139	G	A	15: 76,004,768 (GRCm39)	P636L	possibly damaging	Het
Bmp6	G	T	13: 38,668,896 (GRCm39)	R293L	probably damaging	Het
Cfd	G	A	10: 79,727,447 (GRCm39)		probably null	Het
Csmd1	C	T	8: 15,967,273 (GRCm39)	A3197T	probably damaging	Het
Flii	G	A	11: 60,613,490 (GRCm39)	L166F	probably damaging	Het
Fmn1	T	A	2: 113,195,822 (GRCm39)	N507K	unknown	Het
Foxe1	C	A	4: 46,344,545 (GRCm39)	R118S	possibly damaging	Het
Fras1	T	C	5: 96,840,483 (GRCm39)	V1646A	probably benign	Het
Gm14496	A	G	2: 181,637,711 (GRCm39)	T262A	possibly damaging	Het
Gpbp1	A	T	13: 111,575,658 (GRCm39)	S282T	probably benign	Het
Gria4	A	T	9: 4,462,029 (GRCm39)	N641K	probably damaging	Het
Grin2a	T	G	16: 9,810,327 (GRCm39)	N24T	probably benign	Het
Gstm2	T	G	3: 107,892,992 (GRCm39)	D40A	probably benign	Het
Heatr1	T	A	13: 12,426,143 (GRCm39)	I657N	probably benign	Het
Kmt2b	T	C	7: 30,282,656 (GRCm39)	E1102G	possibly damaging	Het
Macroh2a1	T	C	13: 56,276,146 (GRCm39)	Y39C	probably damaging	Het
Mgam	T	A	6: 40,635,944 (GRCm39)	N366K	probably benign	Het
Mier2	A	T	10: 79,385,510 (GRCm39)	V35E	probably damaging	Het
Mlxipl	T	C	5: 135,161,224 (GRCm39)	F381S	possibly damaging	Het
Mroh1	A	G	15: 76,336,048 (GRCm39)	H1474R	possibly damaging	Het
Mtrf1l	G	T	10: 5,765,620 (GRCm39)	A239E	probably damaging	Het
Nbea	G	T	3: 55,625,200 (GRCm39)	A2297E	probably damaging	Het
Nectin4	A	G	1: 171,207,827 (GRCm39)	E73G	probably benign	Het
Neurl1b	C	G	17: 26,657,720 (GRCm39)	H219Q	probably benign	Het
Nfia	G	A	4: 97,671,742 (GRCm39)	V151I	probably damaging	Het
Nol6	A	G	4: 41,118,717 (GRCm39)	L720P	probably damaging	Het
Or6c203	A	G	10: 129,010,128 (GRCm39)	F254S	probably damaging	Het
Patj	A	G	4: 98,407,179 (GRCm39)	E1054G	probably damaging	Het
Pcnx1	A	G	12: 82,037,325 (GRCm39)	D1861G	probably damaging	Het
Prss12	A	G	3: 123,241,045 (GRCm39)	T80A	probably benign	Het
Scart2	A	G	7: 139,876,234 (GRCm39)	S569G	possibly damaging	Het
Sgsm1	C	A	5: 113,400,890 (GRCm39)	R1000L	probably damaging	Het
Sulf2	T	C	2: 165,935,516 (GRCm39)	D199G	possibly damaging	Het
Suox	T	C	10: 128,506,780 (GRCm39)	D416G	probably benign	Het
Syne1	G	A	10: 5,011,531 (GRCm39)	T38M	probably damaging	Het
Tcf7l2	T	A	19: 55,912,975 (GRCm39)	C421*	probably null	Het
Tex55	C	T	16: 38,648,453 (GRCm39)	D219N	possibly damaging	Het
Traf7	C	A	17: 24,732,282 (GRCm39)	G143*	probably null	Het
Trappc12	T	C	12: 28,761,957 (GRCm39)	I544V	probably benign	Het
Trmt1	G	A	8: 85,424,180 (GRCm39)	V434I	probably benign	Het
Ttc5	T	C	14: 51,014,787 (GRCm39)	H160R	probably benign	Het
Xirp2	T	A	2: 67,342,521 (GRCm39)	H1587Q	probably benign	Het
Zfp523	C	T	17: 28,420,015 (GRCm39)	T220M	probably damaging	Het
Zfp804b	A	T	5: 6,819,362 (GRCm39)	S1234T	probably damaging	Het

Other mutations in Or7e165

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01461:Or7e165	APN	9	19,695,245 (GRCm39)	splice site	probably null
IGL01815:Or7e165	APN	9	19,695,311 (GRCm39)	missense	probably damaging	1.00
IGL02408:Or7e165	APN	9	19,694,711 (GRCm39)	missense	probably benign	0.31
IGL02644:Or7e165	APN	9	19,695,010 (GRCm39)	missense	probably benign	0.30
IGL03243:Or7e165	APN	9	19,694,564 (GRCm39)	missense	probably damaging	0.99
R0603:Or7e165	UTSW	9	19,695,235 (GRCm39)	missense	probably damaging	1.00
R2363:Or7e165	UTSW	9	19,694,892 (GRCm39)	missense	probably benign	0.00
R2570:Or7e165	UTSW	9	19,695,305 (GRCm39)	missense	probably damaging	1.00
R3890:Or7e165	UTSW	9	19,695,011 (GRCm39)	missense	probably benign	0.03
R3892:Or7e165	UTSW	9	19,695,011 (GRCm39)	missense	probably benign	0.03
R4163:Or7e165	UTSW	9	19,695,086 (GRCm39)	missense	possibly damaging	0.69
R4610:Or7e165	UTSW	9	19,694,442 (GRCm39)	nonsense	probably null
R4691:Or7e165	UTSW	9	19,694,678 (GRCm39)	missense	probably benign	0.33
R4707:Or7e165	UTSW	9	19,694,596 (GRCm39)	missense	probably damaging	1.00
R4825:Or7e165	UTSW	9	19,694,872 (GRCm39)	missense	possibly damaging	0.74
R4950:Or7e165	UTSW	9	19,695,027 (GRCm39)	missense	probably benign
R5185:Or7e165	UTSW	9	19,694,672 (GRCm39)	missense	probably damaging	1.00
R5202:Or7e165	UTSW	9	19,694,514 (GRCm39)	missense	possibly damaging	0.46
R5439:Or7e165	UTSW	9	19,695,161 (GRCm39)	missense	probably damaging	1.00
R5669:Or7e165	UTSW	9	19,695,053 (GRCm39)	missense	probably benign	0.02
R5672:Or7e165	UTSW	9	19,694,507 (GRCm39)	missense	possibly damaging	0.92
R6038:Or7e165	UTSW	9	19,694,858 (GRCm39)	missense	probably benign
R6038:Or7e165	UTSW	9	19,694,858 (GRCm39)	missense	probably benign
R6212:Or7e165	UTSW	9	19,694,585 (GRCm39)	missense	probably damaging	1.00
R6415:Or7e165	UTSW	9	19,695,044 (GRCm39)	missense	probably damaging	1.00
R7385:Or7e165	UTSW	9	19,694,507 (GRCm39)	missense	possibly damaging	0.92

Predicted Primers

PCR Primer
(F):5'- GTCTGACATGGGCTTTAGCAG -3'
(R):5'- GCTTGAAAGAAACATGGCTATGTC -3'

Sequencing Primer
(F):5'- TTTAGCAGCACCACAATCCC -3'
(R):5'- ACATGGCTATGTCATTGATAGTAGTG -3'

Posted On

2019-11-12