Mutagenetix > Incidental Mutation

Incidental Mutation 'R8015:Or5g25'

617112

Institutional Source

Beutler Lab

Gene Symbol

Or5g25

Ensembl Gene

ENSMUSG00000075214

Gene Name

olfactory receptor family 5 subfamily G member 25

Synonyms

MOR175-2, Olfr1002, GA_x6K02T2Q125-47127746-47126790

MMRRC Submission

067455-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R8015 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85477707-85478663 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 85478136 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 176 (H176Q)

Ref Sequence

ENSEMBL: ENSMUSP00000150405 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099920] [ENSMUST00000215548]

AlphaFold

Q8VFK2

Predicted Effect

probably damaging
Transcript: ENSMUST00000099920
AA Change: H176Q

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000097504
Gene: ENSMUSG00000075214
AA Change: H176Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	8.7e-50	PFAM
Pfam:7tm_1	41	290	2.5e-17	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215548
AA Change: H176Q

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (51/51)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alad	A	G	4: 62,430,159 (GRCm39)	V121A	probably damaging	Het
Anapc2	T	A	2: 25,174,688 (GRCm39)	Y684N	probably benign	Het
Atp10a	G	T	7: 58,453,245 (GRCm39)	R808L	probably benign	Het
Best2	A	G	8: 85,735,983 (GRCm39)	V317A	probably damaging	Het
Bmal2	T	C	6: 146,722,088 (GRCm39)	S220P	probably damaging	Het
Cacnb4	A	G	2: 52,354,655 (GRCm39)	L241P	probably damaging	Het
Col6a5	T	C	9: 105,758,940 (GRCm39)	T2089A	possibly damaging	Het
Cttnbp2	G	C	6: 18,426,092 (GRCm39)	A762G	possibly damaging	Het
Defb13	G	T	8: 22,436,828 (GRCm39)	V8F	possibly damaging	Het
Dpp6	C	T	5: 27,022,808 (GRCm39)		probably benign	Het
Fat4	A	G	3: 39,036,065 (GRCm39)	D3239G	possibly damaging	Het
Hmcn1	T	G	1: 150,474,062 (GRCm39)	R4793S	possibly damaging	Het
Hspa1a	T	A	17: 35,189,625 (GRCm39)	Q426L	probably damaging	Het
Hycc2	A	G	1: 58,574,641 (GRCm39)	V300A	possibly damaging	Het
Il20rb	T	A	9: 100,356,947 (GRCm39)	Y61F	probably damaging	Het
Iqcn	C	T	8: 71,169,441 (GRCm39)	S1177L	probably benign	Het
Itgb1	T	A	8: 129,448,882 (GRCm39)	V496D	possibly damaging	Het
Jakmip1	T	C	5: 37,317,109 (GRCm39)	S102P	unknown	Het
Jmjd8	T	A	17: 26,048,302 (GRCm39)	F108I	probably damaging	Het
Krtap15-1	A	G	16: 88,626,097 (GRCm39)	T55A	possibly damaging	Het
Lce3d	A	T	3: 92,865,810 (GRCm39)		probably benign	Het
Lnpep	C	A	17: 17,766,761 (GRCm39)	V702F	probably damaging	Het
Lox	G	A	18: 52,661,420 (GRCm39)	A218V	probably benign	Het
Mcrs1	T	A	15: 99,146,735 (GRCm39)	K99*	probably null	Het
Mfsd8	G	A	3: 40,801,270 (GRCm39)		probably benign	Het
Msto1	G	T	3: 88,818,863 (GRCm39)	P264Q	probably damaging	Het
Nrp2	C	T	1: 62,784,567 (GRCm39)	R239C	probably damaging	Het
Or2ad1	T	C	13: 21,326,303 (GRCm39)	D308G	probably benign	Het
Pate2	A	T	9: 35,581,814 (GRCm39)	H36L	probably damaging	Het
Pate9	A	G	9: 36,446,250 (GRCm39)	V54A	probably benign	Het
Phf20l1	T	G	15: 66,511,797 (GRCm39)	N925K	possibly damaging	Het
Pnliprp2	G	T	19: 58,754,714 (GRCm39)	V253F	probably damaging	Het
Polr2b	A	G	5: 77,484,353 (GRCm39)	D745G	probably damaging	Het
Ptdss1	T	A	13: 67,111,407 (GRCm39)	W158R	possibly damaging	Het
Rcn3	T	A	7: 44,734,331 (GRCm39)	I226F	probably damaging	Het
Rhbdl1	C	G	17: 26,054,825 (GRCm39)	V157L	probably damaging	Het
Slc30a2	A	G	4: 134,074,761 (GRCm39)	T173A	probably benign	Het
Spata16	A	G	3: 26,721,808 (GRCm39)	T110A	probably benign	Het
Specc1l	A	G	10: 75,076,902 (GRCm39)	K53E	probably benign	Het
Speg	G	T	1: 75,392,065 (GRCm39)		probably benign	Het
Spta1	T	A	1: 174,067,737 (GRCm39)	C2055S	probably damaging	Het
Tex14	G	A	11: 87,400,426 (GRCm39)	R406Q	probably benign	Het
Tmem150b	C	T	7: 4,719,327 (GRCm39)	G198S	probably null	Het
Tnfrsf14	T	G	4: 155,011,118 (GRCm39)	Q81P	probably damaging	Het
Txndc17	A	G	11: 72,098,568 (GRCm39)	K40R	probably benign	Het
Ube3a	T	A	7: 58,934,504 (GRCm39)	V560E	probably damaging	Het
Vmn1r14	A	G	6: 57,211,015 (GRCm39)	M198V	probably damaging	Het
Vmn2r23	G	A	6: 123,681,500 (GRCm39)	S136N	probably benign	Het
Vmn2r31	C	T	7: 7,387,199 (GRCm39)	V791I	probably damaging	Het
Wdfy4	T	C	14: 32,829,704 (GRCm39)	Y984C		Het
Zfp386	T	G	12: 116,023,027 (GRCm39)	D248E	probably damaging	Het

Other mutations in Or5g25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02591:Or5g25	APN	2	85,478,487 (GRCm39)	missense	probably damaging	0.98
IGL02873:Or5g25	APN	2	85,478,096 (GRCm39)	missense	possibly damaging	0.50
PIT4362001:Or5g25	UTSW	2	85,478,068 (GRCm39)	missense	probably damaging	1.00
R1241:Or5g25	UTSW	2	85,477,904 (GRCm39)	missense	probably damaging	1.00
R1261:Or5g25	UTSW	2	85,478,143 (GRCm39)	missense	probably damaging	1.00
R1666:Or5g25	UTSW	2	85,478,157 (GRCm39)	nonsense	probably null
R1902:Or5g25	UTSW	2	85,478,201 (GRCm39)	missense	possibly damaging	0.81
R1965:Or5g25	UTSW	2	85,478,090 (GRCm39)	missense	possibly damaging	0.94
R2096:Or5g25	UTSW	2	85,478,434 (GRCm39)	missense	probably benign	0.20
R4239:Or5g25	UTSW	2	85,478,647 (GRCm39)	missense	probably damaging	0.98
R4730:Or5g25	UTSW	2	85,478,336 (GRCm39)	missense	probably benign	0.39
R4948:Or5g25	UTSW	2	85,477,916 (GRCm39)	missense	probably benign	0.30
R5627:Or5g25	UTSW	2	85,477,991 (GRCm39)	missense	probably damaging	1.00
R5844:Or5g25	UTSW	2	85,478,239 (GRCm39)	missense	probably benign	0.36
R6809:Or5g25	UTSW	2	85,478,317 (GRCm39)	missense	probably damaging	1.00
R7399:Or5g25	UTSW	2	85,477,768 (GRCm39)	missense	possibly damaging	0.89
R7476:Or5g25	UTSW	2	85,478,512 (GRCm39)	missense	not run
R7805:Or5g25	UTSW	2	85,477,794 (GRCm39)	nonsense	probably null
R7960:Or5g25	UTSW	2	85,478,417 (GRCm39)	missense	possibly damaging	0.82
R8355:Or5g25	UTSW	2	85,478,485 (GRCm39)	missense	probably damaging	1.00
R8455:Or5g25	UTSW	2	85,478,485 (GRCm39)	missense	probably damaging	1.00
R8479:Or5g25	UTSW	2	85,478,447 (GRCm39)	missense	probably damaging	1.00
R8683:Or5g25	UTSW	2	85,478,410 (GRCm39)	missense	probably benign	0.35
R8699:Or5g25	UTSW	2	85,478,330 (GRCm39)	missense	possibly damaging	0.87
R8762:Or5g25	UTSW	2	85,478,034 (GRCm39)	missense	probably damaging	1.00
R8897:Or5g25	UTSW	2	85,478,187 (GRCm39)	missense	possibly damaging	0.92
R9280:Or5g25	UTSW	2	85,478,504 (GRCm39)	nonsense	probably null
R9674:Or5g25	UTSW	2	85,478,593 (GRCm39)	missense	possibly damaging	0.94

Predicted Primers

PCR Primer
(F):5'- TGCTGTCAGGTGTGAAGAGC -3'
(R):5'- ACAGAATGTTTCCTCCTGGC -3'

Sequencing Primer
(F):5'- CAGGTGGAGAAGGCTTTGC -3'
(R):5'- AATGTTTCCTCCTGGCCTCCATG -3'

Posted On

2020-01-23