|Institutional Source||Beutler Lab|
|Gene Name||protein phosphatase 2, regulatory subunit A, alpha|
|Synonyms||PR65, PP2A, 6330556D22Rik, protein phosphatase PP2A|
|Essential gene?||Essential (E-score: 1.000)|
|Stock #||R9712 (G1)|
|Chromosomal Location||20945311-20965916 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to C at 20958796 bp (GRCm38)|
|Amino Acid Change||Glutamic Acid to Alanine at position 295 (E295A)|
|Ref Sequence||ENSEMBL: ENSMUSP00000007708 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000007708] [ENSMUST00000147983] [ENSMUST00000173658]|
Crystal structure of a protein phosphatase 2A (PP2A) holoenzyme. [X-RAY DIFFRACTION]
Crystal structure of the full-length simian virus 40 small t antigen complexed with the protein phosphatase 2A Aalpha subunit [X-RAY DIFFRACTION]
Structural Basis of PP2A and Sgo interaction [X-RAY DIFFRACTION]
AA Change: E295A
PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)
AA Change: E295A
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a targeted allele that remove exons 5 and 6 exhibit embryonic lethality. Mice heterozygous for this allele exhibit increased benzopyrene-induced lung tumors. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ppp2r1a||
(F):5'- ACTATGTCCCTTGTGCTGCG -3'
(R):5'- CAGGGCAAGATCTGAGTCATG -3'
(F):5'- GCGTCCTAACCTTATTCTGGTG -3'
(R):5'- GAGTCATGATCACATTCTCCCGG -3'