Mutagenetix > Incidental Mutation

Incidental Mutation 'R9712:Fat1'

730257

Institutional Source

Beutler Lab

Gene Symbol

Fat1

Ensembl Gene

ENSMUSG00000070047

Gene Name

FAT atypical cadherin 1

Synonyms

mFat1, Fath, 2310038E12Rik

MMRRC Submission

Accession Numbers

Genbank: NM_001081286

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9712 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

44935447-45052257 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 45017380 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 1472 (I1472L)

Ref Sequence

ENSEMBL: ENSMUSP00000149194 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098796] [ENSMUST00000189017] [ENSMUST00000191428] [ENSMUST00000215588]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000098796
AA Change: I1449L

PolyPhen 2 Score 0.064 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000096394
Gene: ENSMUSG00000070047
AA Change: I1449L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000189017
AA Change: I1449L

Predicted Effect

probably benign
Transcript: ENSMUST00000191428
AA Change: I1449L

PolyPhen 2 Score 0.064 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000140596
Gene: ENSMUSG00000070047
AA Change: I1449L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000215588
AA Change: I1472L

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit holoprosencephaly, anophthalmia, kidney defects and perinatal lethality. Mice homozygous for a hypomorphic allele exhibit altered shoulder girdle and facial musculature, retinal defects, abnormal inner earpatterning and kidney defects. [provided by MGI curators]

Allele List at MGI

All alleles(56) : Targeted, other(1) Gene trapped(55)

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810055G02Rik	A	T	19: 3,715,784	M20L	probably benign	Het
9430007A20Rik	C	T	4: 144,528,784	A258V	probably benign	Het
Abca8b	T	A	11: 109,942,337	D1241V	probably benign	Het
Adam20	G	A	8: 40,795,453	R200H	probably benign	Het
Adcy10	T	C	1: 165,513,112	F229L	probably damaging	Het
Adsl	A	T	15: 80,955,639	N126I	probably benign	Het
Ahnak	AGATCTC	A	19: 9,007,028		probably benign	Het
Ahnak	GATCTCTAT	GAT	19: 9,007,029		probably benign	Het
Aktip	G	A	8: 91,129,727	P41S	probably damaging	Het
Alkbh3	C	A	2: 93,980,973	R258L	probably damaging	Het
Arfgap3	A	G	15: 83,313,533	Y341H	probably benign	Het
Arhgef26	C	T	3: 62,423,613	L583F	probably damaging	Het
Arhgef40	T	C	14: 51,988,958	I153T	probably damaging	Het
Atp1a1	T	C	3: 101,591,441	S179G	probably benign	Het
Bok	T	C	1: 93,686,507	S21P	probably damaging	Het
C130060K24Rik	A	G	6: 65,456,140	I315V	probably benign	Het
Ccdc136	A	G	6: 29,417,442	E754G	probably benign	Het
Cers3	A	G	7: 66,773,630	K108E	probably benign	Het
Cmya5	T	C	13: 93,065,373		probably null	Het
Col19a1	T	C	1: 24,328,067	E478G	possibly damaging	Het
Colec10	T	A	15: 54,459,784	S134R	possibly damaging	Het
Ctnnb1	A	T	9: 120,955,829	I514F	probably damaging	Het
Cux1	C	T	5: 136,309,819	E664K	probably benign	Het
Dars	T	C	1: 128,405,462	Q75R	probably benign	Het
Disp1	T	A	1: 183,135,815	S16C	probably damaging	Het
Dnah7a	A	T	1: 53,559,140	V1412E	probably benign	Het
Ect2l	C	T	10: 18,168,434	V226I	probably benign	Het
Ep400	T	A	5: 110,756,643	H30L	unknown	Het
Ephx4	A	G	5: 107,419,781	I202V	probably benign	Het
Exoc3	A	T	13: 74,192,908	F259Y	probably damaging	Het
Ezr	T	C	17: 6,752,995	E229G	probably damaging	Het
Fsd1l	T	G	4: 53,679,972	D223E	probably benign	Het
Gata6	A	T	18: 11,059,064	D377V	possibly damaging	Het
Gm12185	A	T	11: 48,907,389	M759K	probably benign	Het
Gm21994	C	T	2: 150,254,576	R311Q	probably benign	Het
Gm9922	C	T	14: 101,729,457	A120T	unknown	Het
Hectd4	A	T	5: 121,310,681	Y364F	probably benign	Het
Hnrnph1	A	G	11: 50,385,869	S465G	unknown	Het
Ifi204	T	C	1: 173,749,358	Y559C	probably damaging	Het
Ift88	T	C	14: 57,481,396	S613P	probably damaging	Het
Kif21a	G	C	15: 90,985,325	A441G	probably damaging	Het
Kif21a	G	T	15: 90,995,512	T191K	probably benign	Het
Lrit1	T	A	14: 37,060,127	C252*	probably null	Het
Ncbp1	T	A	4: 46,144,837	D29E	probably benign	Het
Nlrp10	A	T	7: 108,925,528	D248E	probably damaging	Het
Nphp4	T	G	4: 152,547,064	V807G	probably benign	Het
Nsd1	T	C	13: 55,246,043	S589P	possibly damaging	Het
Oas1b	T	A	5: 120,814,485	N80K	probably damaging	Het
Olfr1055	G	A	2: 86,347,239	H176Y	probably benign	Het
Olfr1135	A	T	2: 87,671,761	I202N	probably benign	Het
Oprk1	T	A	1: 5,598,873	C181S	probably damaging	Het
Oprl1	T	A	2: 181,718,419	N89K	probably damaging	Het
Pdcd11	A	G	19: 47,129,302	K1697E	probably damaging	Het
Pdp1	G	A	4: 11,961,607	H254Y	probably benign	Het
Pds5b	A	T	5: 150,805,663	D1419V	possibly damaging	Het
Per1	G	T	11: 69,100,649	G3V	probably benign	Het
Pex16	C	A	2: 92,376,643	N55K	probably damaging	Het
Phldb2	C	A	16: 45,774,977	L862F	probably benign	Het
Pld5	T	A	1: 175,964,006	D478V	probably benign	Het
Pms2	T	C	5: 143,914,796	I177T	probably damaging	Het
Pou1f1	A	T	16: 65,529,872	E119D	probably benign	Het
Ppp2r1a	A	C	17: 20,958,796	E295A	probably damaging	Het
Rad51ap2	A	T	12: 11,457,592	N505I	possibly damaging	Het
Rasal3	A	C	17: 32,396,562	V434G	probably damaging	Het
Rasgrf2	C	T	13: 91,987,973	V6M		Het
Rwdd1	A	T	10: 34,001,156	D197E		Het
Scn11a	A	T	9: 119,790,010	C755*	probably null	Het
Skil	T	C	3: 31,116,860	S443P	probably benign	Het
Slc12a6	T	C	2: 112,356,472	C939R	probably damaging	Het
Slc25a36	A	G	9: 97,079,177	S269P	probably benign	Het
Srrm4	T	A	5: 116,482,393	H92L	unknown	Het
Styk1	CTCTTCATGATTTTCTT	CTCTT	6: 131,301,649		probably benign	Het
Tbc1d4	A	T	14: 101,507,410	V260E	probably benign	Het
Tbc1d8	T	C	1: 39,385,232	N593D	probably damaging	Het
Trex1	C	A	9: 109,058,737	R62L	probably damaging	Het
Trpm3	A	T	19: 22,715,352	D269V	possibly damaging	Het
Trpv4	G	T	5: 114,633,150	Y439*	probably null	Het
Ttn	G	A	2: 76,734,248	T28515I	probably damaging	Het
Ucn2	G	A	9: 108,986,503	G111D	probably damaging	Het
Uhrf2	A	G	19: 30,056,481	I212V	possibly damaging	Het
Usp24	T	A	4: 106,347,367	M261K	probably benign	Het
Vmn1r235	A	T	17: 21,261,698	D95V	probably benign	Het
Vwf	G	A	6: 125,624,573	R826Q		Het
Zfp28	T	C	7: 6,393,879	C438R	probably damaging	Het

Other mutations in Fat1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Fat1	APN	8	45024602	missense	possibly damaging	0.93
IGL00157:Fat1	APN	8	44951670	missense	possibly damaging	0.96
IGL00481:Fat1	APN	8	45050940	missense	probably benign	0.18
IGL00983:Fat1	APN	8	45033390	missense	probably damaging	1.00
IGL01089:Fat1	APN	8	45017857	missense	probably damaging	1.00
IGL01135:Fat1	APN	8	45024840	missense	probably damaging	1.00
IGL01143:Fat1	APN	8	45035532	missense	possibly damaging	0.72
IGL01155:Fat1	APN	8	45023949	missense	probably damaging	1.00
IGL01376:Fat1	APN	8	45026841	missense	probably benign	0.00
IGL01411:Fat1	APN	8	45026800	missense	probably damaging	1.00
IGL01443:Fat1	APN	8	45040576	missense	probably damaging	1.00
IGL01453:Fat1	APN	8	45051270	missense	probably damaging	1.00
IGL01606:Fat1	APN	8	45023049	missense	probably benign	0.26
IGL01622:Fat1	APN	8	45029555	missense	possibly damaging	0.64
IGL01623:Fat1	APN	8	45029555	missense	possibly damaging	0.64
IGL01672:Fat1	APN	8	45040700	missense	probably benign	0.05
IGL01735:Fat1	APN	8	45036239	missense	probably benign	0.07
IGL01793:Fat1	APN	8	44989112	missense	probably benign
IGL01820:Fat1	APN	8	45010502	missense	probably damaging	1.00
IGL01969:Fat1	APN	8	44952599	missense	probably damaging	0.98
IGL02012:Fat1	APN	8	45027540	missense	possibly damaging	0.95
IGL02227:Fat1	APN	8	45023659	missense	probably damaging	1.00
IGL02256:Fat1	APN	8	44950332	missense	probably damaging	1.00
IGL02273:Fat1	APN	8	44950331	missense	probably damaging	1.00
IGL02317:Fat1	APN	8	45025818	missense	probably benign	0.33
IGL02324:Fat1	APN	8	45040556	missense	probably damaging	1.00
IGL02336:Fat1	APN	8	44951583	missense	probably benign	0.16
IGL02442:Fat1	APN	8	44950323	missense	probably benign	0.02
IGL02486:Fat1	APN	8	45025072	missense	probably benign	0.16
IGL02551:Fat1	APN	8	45051398	missense	probably damaging	1.00
IGL02617:Fat1	APN	8	45035591	missense	probably benign	0.31
IGL02698:Fat1	APN	8	45023164	missense	probably benign
IGL02885:Fat1	APN	8	44989167	missense	probably benign	0.01
IGL02904:Fat1	APN	8	45040682	missense	probably damaging	1.00
IGL02953:Fat1	APN	8	45024314	missense	probably damaging	1.00
IGL03108:Fat1	APN	8	45023614	missense	probably damaging	1.00
IGL03153:Fat1	APN	8	45030123	missense	possibly damaging	0.83
IGL03183:Fat1	APN	8	44950586	missense	probably damaging	0.99
IGL03327:Fat1	APN	8	44950468	missense	probably damaging	1.00
IGL03405:Fat1	APN	8	45025241	missense	probably damaging	1.00
Laggardly	UTSW	8	45044464	missense	probably damaging	1.00
R2257_fat1_465	UTSW	8	44950371	missense	probably damaging	1.00
Shrinkage	UTSW	8	45018037	missense	probably damaging	1.00
F5493:Fat1	UTSW	8	45025480	missense	probably damaging	0.99
G1citation:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
I2289:Fat1	UTSW	8	45024996	missense	probably benign	0.01
IGL02837:Fat1	UTSW	8	45017434	missense	probably benign	0.00
PIT4283001:Fat1	UTSW	8	45029540	missense	probably damaging	1.00
PIT4283001:Fat1	UTSW	8	45037207	missense	probably damaging	1.00
PIT4576001:Fat1	UTSW	8	45024645	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
R0078:Fat1	UTSW	8	44953299	missense	probably damaging	1.00
R0197:Fat1	UTSW	8	45026553	missense	probably benign	0.00
R0328:Fat1	UTSW	8	45023790	missense	probably benign	0.35
R0367:Fat1	UTSW	8	45024313	missense	probably damaging	1.00
R0371:Fat1	UTSW	8	44951892	missense	probably damaging	1.00
R0380:Fat1	UTSW	8	45010123	missense	probably damaging	0.97
R0389:Fat1	UTSW	8	44950348	missense	probably benign	0.00
R0433:Fat1	UTSW	8	45024649	missense	possibly damaging	0.51
R0456:Fat1	UTSW	8	45029534	missense	probably damaging	1.00
R0494:Fat1	UTSW	8	44950542	missense	probably damaging	1.00
R0506:Fat1	UTSW	8	45022951	missense	probably damaging	0.99
R0512:Fat1	UTSW	8	44951332	nonsense	probably null
R0624:Fat1	UTSW	8	45051168	missense	possibly damaging	0.46
R0701:Fat1	UTSW	8	45026553	missense	probably benign	0.00
R0723:Fat1	UTSW	8	45026749	missense	probably damaging	1.00
R0787:Fat1	UTSW	8	45040555	missense	probably damaging	1.00
R0788:Fat1	UTSW	8	45023983	missense	probably benign	0.27
R0862:Fat1	UTSW	8	45018037	missense	probably damaging	1.00
R0864:Fat1	UTSW	8	45018037	missense	probably damaging	1.00
R0907:Fat1	UTSW	8	45026598	missense	probably benign	0.08
R0962:Fat1	UTSW	8	45033326	splice site	probably benign
R1051:Fat1	UTSW	8	45044506	missense	probably damaging	1.00
R1156:Fat1	UTSW	8	45039890	missense	possibly damaging	0.94
R1237:Fat1	UTSW	8	45044279	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45010545	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45010545	missense	probably damaging	1.00
R1478:Fat1	UTSW	8	45025622	missense	probably damaging	0.99
R1482:Fat1	UTSW	8	44953244	missense	probably benign	0.04
R1496:Fat1	UTSW	8	45033390	missense	probably damaging	1.00
R1498:Fat1	UTSW	8	45025484	nonsense	probably null
R1508:Fat1	UTSW	8	45026862	missense	probably benign	0.01
R1577:Fat1	UTSW	8	45023383	missense	probably benign	0.30
R1646:Fat1	UTSW	8	45018042	missense	probably damaging	1.00
R1652:Fat1	UTSW	8	45025178	nonsense	probably null
R1656:Fat1	UTSW	8	45025530	nonsense	probably null
R1662:Fat1	UTSW	8	44953164	missense	probably benign	0.20
R1672:Fat1	UTSW	8	45036835	missense	probably damaging	1.00
R1704:Fat1	UTSW	8	45025576	missense	probably damaging	1.00
R1708:Fat1	UTSW	8	45024792	missense	probably damaging	1.00
R1710:Fat1	UTSW	8	45010482	missense	probably benign	0.00
R1812:Fat1	UTSW	8	45036803	missense	probably damaging	1.00
R1872:Fat1	UTSW	8	44953304	missense	probably benign	0.01
R1872:Fat1	UTSW	8	45038349	missense	probably damaging	1.00
R1883:Fat1	UTSW	8	45051147	missense	probably benign	0.17
R1893:Fat1	UTSW	8	45023856	missense	probably damaging	1.00
R1930:Fat1	UTSW	8	45044228	missense	possibly damaging	0.91
R1931:Fat1	UTSW	8	45044228	missense	possibly damaging	0.91
R1952:Fat1	UTSW	8	45033926	missense	probably benign	0.00
R1957:Fat1	UTSW	8	45040682	missense	probably damaging	1.00
R1999:Fat1	UTSW	8	44952393	missense	probably damaging	0.96
R2019:Fat1	UTSW	8	45023746	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45024332	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45026704	missense	probably damaging	1.00
R2117:Fat1	UTSW	8	45037463	missense	probably benign	0.33
R2196:Fat1	UTSW	8	45024646	missense	probably damaging	1.00
R2204:Fat1	UTSW	8	45023700	missense	probably damaging	1.00
R2256:Fat1	UTSW	8	44950371	missense	probably damaging	1.00
R2257:Fat1	UTSW	8	44950371	missense	probably damaging	1.00
R2409:Fat1	UTSW	8	45040530	splice site	probably benign
R2416:Fat1	UTSW	8	45026383	missense	probably damaging	1.00
R3021:Fat1	UTSW	8	45044011	missense	probably damaging	1.00
R3108:Fat1	UTSW	8	45045173	splice site	probably null
R3109:Fat1	UTSW	8	45045173	splice site	probably null
R3196:Fat1	UTSW	8	44951868	missense	probably benign	0.00
R3683:Fat1	UTSW	8	45017938	missense	probably benign
R3732:Fat1	UTSW	8	44953269	missense	possibly damaging	0.85
R3732:Fat1	UTSW	8	44953269	missense	possibly damaging	0.85
R3733:Fat1	UTSW	8	44953269	missense	possibly damaging	0.85
R3753:Fat1	UTSW	8	45025479	missense	probably damaging	0.97
R3905:Fat1	UTSW	8	45023035	missense	probably benign	0.00
R3907:Fat1	UTSW	8	45023035	missense	probably benign	0.00
R3908:Fat1	UTSW	8	45023035	missense	probably benign	0.00
R4060:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4061:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4062:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4063:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4078:Fat1	UTSW	8	44989122	missense	probably damaging	0.99
R4105:Fat1	UTSW	8	45036851	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45010437	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45050944	missense	probably damaging	1.00
R4161:Fat1	UTSW	8	45036787	missense	probably benign	0.00
R4364:Fat1	UTSW	8	44952962	missense	probably benign	0.01
R4394:Fat1	UTSW	8	44952346	missense	probably damaging	0.98
R4395:Fat1	UTSW	8	44952346	missense	probably damaging	0.98
R4396:Fat1	UTSW	8	44952346	missense	probably damaging	0.98
R4412:Fat1	UTSW	8	45023599	missense	probably damaging	0.99
R4542:Fat1	UTSW	8	45041894	missense	probably damaging	1.00
R4591:Fat1	UTSW	8	45026242	missense	probably benign
R4606:Fat1	UTSW	8	44950683	missense	possibly damaging	0.47
R4612:Fat1	UTSW	8	45025147	missense	probably damaging	1.00
R4730:Fat1	UTSW	8	45033477	missense	probably damaging	1.00
R4778:Fat1	UTSW	8	45038326	missense	probably benign	0.04
R4824:Fat1	UTSW	8	44989114	missense	probably damaging	1.00
R4829:Fat1	UTSW	8	45036162	missense	probably damaging	1.00
R4832:Fat1	UTSW	8	45013065	missense	possibly damaging	0.95
R4849:Fat1	UTSW	8	45012970	missense	probably benign	0.15
R4896:Fat1	UTSW	8	44951280	missense	possibly damaging	0.68
R4927:Fat1	UTSW	8	45022963	missense	probably damaging	0.96
R4941:Fat1	UTSW	8	45036275	missense	probably benign	0.00
R5011:Fat1	UTSW	8	45031263	critical splice acceptor site	probably null
R5040:Fat1	UTSW	8	45023380	missense	probably damaging	1.00
R5112:Fat1	UTSW	8	45024282	missense	probably damaging	1.00
R5151:Fat1	UTSW	8	44951814	missense	possibly damaging	0.74
R5161:Fat1	UTSW	8	44952512	missense	probably benign	0.00
R5162:Fat1	UTSW	8	45025809	missense	probably benign	0.02
R5353:Fat1	UTSW	8	45036131	missense	probably benign	0.13
R5425:Fat1	UTSW	8	45025885	missense	possibly damaging	0.64
R5458:Fat1	UTSW	8	45013053	missense	probably damaging	1.00
R5479:Fat1	UTSW	8	45036875	missense	possibly damaging	0.88
R5543:Fat1	UTSW	8	45023479	missense	probably damaging	0.99
R5569:Fat1	UTSW	8	45039836	missense	probably damaging	0.98
R5610:Fat1	UTSW	8	44953072	nonsense	probably null
R5734:Fat1	UTSW	8	45051209	missense	probably damaging	0.99
R5832:Fat1	UTSW	8	45017423	missense	possibly damaging	0.65
R5860:Fat1	UTSW	8	45051129	missense	probably benign
R5886:Fat1	UTSW	8	45027681	critical splice donor site	probably null
R5886:Fat1	UTSW	8	45033395	missense	probably damaging	1.00
R5919:Fat1	UTSW	8	45026873	critical splice donor site	probably null
R5930:Fat1	UTSW	8	45044036	missense	probably benign	0.10
R5960:Fat1	UTSW	8	45033368	missense	probably damaging	1.00
R5988:Fat1	UTSW	8	45029456	missense	probably benign	0.00
R6166:Fat1	UTSW	8	44952485	missense	probably damaging	1.00
R6184:Fat1	UTSW	8	44953392	missense	probably benign	0.00
R6208:Fat1	UTSW	8	45027613	missense	probably damaging	0.99
R6351:Fat1	UTSW	8	45033495	missense	probably damaging	1.00
R6391:Fat1	UTSW	8	44952342	missense	possibly damaging	0.69
R6701:Fat1	UTSW	8	44950681	missense	probably damaging	1.00
R6702:Fat1	UTSW	8	44953046	missense	probably benign	0.28
R6703:Fat1	UTSW	8	44953046	missense	probably benign	0.28
R6704:Fat1	UTSW	8	45024373	missense	probably damaging	1.00
R6822:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
R6852:Fat1	UTSW	8	45035598	missense	possibly damaging	0.46
R6863:Fat1	UTSW	8	45044464	missense	probably damaging	1.00
R6885:Fat1	UTSW	8	44952452	missense	possibly damaging	0.94
R6912:Fat1	UTSW	8	45051023	missense	probably benign	0.00
R6927:Fat1	UTSW	8	45024495	missense	probably benign	0.41
R6964:Fat1	UTSW	8	45043945	missense	probably damaging	1.00
R7010:Fat1	UTSW	8	44953349	nonsense	probably null
R7062:Fat1	UTSW	8	44950216	start codon destroyed	probably null	0.99
R7063:Fat1	UTSW	8	45040775	missense	probably benign	0.09
R7071:Fat1	UTSW	8	44989108	missense	possibly damaging	0.67
R7117:Fat1	UTSW	8	45031468	missense	probably damaging	0.98
R7146:Fat1	UTSW	8	44950925	missense	probably benign
R7210:Fat1	UTSW	8	45023503	missense	probably damaging	1.00
R7227:Fat1	UTSW	8	45010609	missense	probably benign	0.08
R7270:Fat1	UTSW	8	45037438	missense	probably damaging	1.00
R7373:Fat1	UTSW	8	45026665	missense	probably damaging	1.00
R7390:Fat1	UTSW	8	44952474	missense	possibly damaging	0.81
R7465:Fat1	UTSW	8	45044152	missense	probably benign	0.35
R7476:Fat1	UTSW	8	45031274	missense	probably benign	0.01
R7483:Fat1	UTSW	8	45023160	missense	probably benign	0.13
R7484:Fat1	UTSW	8	45036184	missense	probably damaging	1.00
R7526:Fat1	UTSW	8	45023427	missense	probably damaging	1.00
R7549:Fat1	UTSW	8	44988994	missense	probably benign	0.01
R7554:Fat1	UTSW	8	45037165	missense	possibly damaging	0.88
R7620:Fat1	UTSW	8	45009850	missense	possibly damaging	0.95
R7652:Fat1	UTSW	8	44953299	missense	probably damaging	1.00
R7694:Fat1	UTSW	8	44988930	critical splice acceptor site	probably null
R7746:Fat1	UTSW	8	44951633	missense	probably damaging	0.96
R7762:Fat1	UTSW	8	45023322	missense	probably damaging	1.00
R7762:Fat1	UTSW	8	45037337	missense	probably damaging	0.99
R7782:Fat1	UTSW	8	44950911	missense	probably damaging	1.00
R7801:Fat1	UTSW	8	45042223	missense	probably damaging	1.00
R7807:Fat1	UTSW	8	45041973	missense	probably damaging	1.00
R7821:Fat1	UTSW	8	44950224	missense	probably benign
R7869:Fat1	UTSW	8	45051222	missense	probably benign	0.02
R8034:Fat1	UTSW	8	44951691	missense	probably benign	0.28
R8094:Fat1	UTSW	8	44952702	missense	probably damaging	0.98
R8111:Fat1	UTSW	8	45026058	missense	possibly damaging	0.94
R8220:Fat1	UTSW	8	45039956	missense	probably null
R8221:Fat1	UTSW	8	44953353	missense
R8233:Fat1	UTSW	8	44952018	missense
R8250:Fat1	UTSW	8	44953299	missense	probably damaging	1.00
R8279:Fat1	UTSW	8	45030347	critical splice donor site	probably null
R8726:Fat1	UTSW	8	45024169	missense	probably benign	0.23
R8875:Fat1	UTSW	8	45040563	missense	probably damaging	1.00
R8937:Fat1	UTSW	8	45030313	missense	probably damaging	1.00
R8950:Fat1	UTSW	8	45023121	missense	probably damaging	1.00
R8971:Fat1	UTSW	8	45042294	missense	probably damaging	1.00
R8976:Fat1	UTSW	8	45031295	missense	probably benign	0.02
R9000:Fat1	UTSW	8	45044550	nonsense	probably null
R9032:Fat1	UTSW	8	45039857	missense	probably benign	0.01
R9076:Fat1	UTSW	8	45039901	missense	probably damaging	1.00
R9083:Fat1	UTSW	8	45013090	missense	possibly damaging	0.76
R9083:Fat1	UTSW	8	45038299	missense	probably benign	0.00
R9103:Fat1	UTSW	8	44951813	missense	probably benign	0.38
R9124:Fat1	UTSW	8	44950326	missense	probably benign
R9124:Fat1	UTSW	8	45025027	missense	possibly damaging	0.48
R9128:Fat1	UTSW	8	45009841	missense	probably benign	0.14
R9148:Fat1	UTSW	8	44952645	missense	possibly damaging	0.81
R9162:Fat1	UTSW	8	44951315	missense	probably damaging	1.00
R9209:Fat1	UTSW	8	44951754	missense	possibly damaging	0.80
R9276:Fat1	UTSW	8	45035477	missense	probably damaging	0.99
R9303:Fat1	UTSW	8	45010461	missense	probably damaging	1.00
R9319:Fat1	UTSW	8	44953023	missense	probably damaging	1.00
R9392:Fat1	UTSW	8	45023191	missense	probably damaging	1.00
R9616:Fat1	UTSW	8	44953038	missense	probably damaging	0.99
R9756:Fat1	UTSW	8	45043937	missense	probably damaging	0.96
RF001:Fat1	UTSW	8	44988966	missense	probably benign	0.00
X0064:Fat1	UTSW	8	45025734	missense	possibly damaging	0.58
Z1088:Fat1	UTSW	8	45023807	missense	possibly damaging	0.88
Z1176:Fat1	UTSW	8	44950598	missense	probably benign
Z1176:Fat1	UTSW	8	45023596	missense	possibly damaging	0.65
Z1176:Fat1	UTSW	8	45036838	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AATTCGCCTGTATTTCTCAAGC -3'
(R):5'- AGGCGGAATTTCTTGAGGC -3'

Sequencing Primer
(F):5'- CCTGTATTTCTCAAGCAATTTTCAAC -3'
(R):5'- CTGGCTGGATCGATGCTGC -3'

Posted On

2022-10-06