Mutagenetix > Incidental Mutation

Incidental Mutation 'R7563:Vmn2r114'

585281

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r114

Ensembl Gene

ENSMUSG00000091945

Gene Name

vomeronasal 2, receptor 114

Synonyms

EG666002

MMRRC Submission

045655-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R7563 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

23509908-23531287 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 23510000 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 827 (V827I)

Ref Sequence

ENSEMBL: ENSMUSP00000127505 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168033]

AlphaFold

E9Q281

Predicted Effect

probably benign
Transcript: ENSMUST00000168033
AA Change: V827I

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000127505
Gene: ENSMUSG00000091945
AA Change: V827I

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	470	1.5e-24	PFAM
Pfam:NCD3G	511	564	1.5e-18	PFAM
Pfam:7tm_3	597	832	1.4e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (56/56)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl3	A	G	4: 144,184,464 (GRCm39)	I98T	probably damaging	Het
Ahnak	T	C	19: 8,988,529 (GRCm39)	I3271T	probably damaging	Het
Aox1	G	A	1: 58,086,304 (GRCm39)	V70I	probably benign	Het
Ap1g2	A	G	14: 55,337,206 (GRCm39)	S710P	probably damaging	Het
Bhlhe40	TG	TGG	6: 108,641,818 (GRCm39)	254	probably null	Het
Cacna1e	T	C	1: 154,347,162 (GRCm39)	K1064E	probably benign	Het
Capn11	T	A	17: 45,944,891 (GRCm39)	I459F	probably damaging	Het
Ccnc	A	G	4: 21,732,220 (GRCm39)	I48V	probably damaging	Het
Ces1d	T	A	8: 93,904,667 (GRCm39)	I358F	probably benign	Het
Clgn	A	T	8: 84,147,185 (GRCm39)	N379I	probably damaging	Het
Cym	T	C	3: 107,121,548 (GRCm39)	Y248C	probably damaging	Het
Epha8	T	C	4: 136,666,100 (GRCm39)	D352G	possibly damaging	Het
Eya2	T	C	2: 165,558,050 (GRCm39)		probably null	Het
Fam219a	A	C	4: 41,569,208 (GRCm39)	V10G	probably benign	Het
Fbxl5	C	T	5: 43,978,891 (GRCm39)	V20I	probably benign	Het
Fbxl9	A	G	8: 106,042,388 (GRCm39)	C147R	probably benign	Het
Fscb	T	C	12: 64,520,059 (GRCm39)	E469G	possibly damaging	Het
Glt8d2	A	T	10: 82,496,659 (GRCm39)		probably null	Het
Grep1	A	T	17: 23,936,302 (GRCm39)	F8L	probably benign	Het
Helt	T	C	8: 46,746,630 (GRCm39)		probably benign	Het
Igkv8-27	G	T	6: 70,148,887 (GRCm39)	T89K	probably benign	Het
Ipo5	T	A	14: 121,183,567 (GRCm39)	H1048Q	probably benign	Het
Kalrn	T	C	16: 34,212,464 (GRCm39)	D28G	probably damaging	Het
Kcnh4	G	A	11: 100,632,680 (GRCm39)	P936S	probably benign	Het
Klrd1	A	G	6: 129,570,701 (GRCm39)	I37M	possibly damaging	Het
Kmt2e	T	C	5: 23,705,271 (GRCm39)	V1267A	probably damaging	Het
Marchf1	A	T	8: 66,920,965 (GRCm39)	Q214L	probably damaging	Het
Mlip	G	T	9: 77,020,279 (GRCm39)	H52N	probably damaging	Het
Oas1e	T	C	5: 120,927,021 (GRCm39)	R229G	probably benign	Het
Ogfr	T	A	2: 180,234,300 (GRCm39)		probably null	Het
Or4g16	T	C	2: 111,137,134 (GRCm39)	F195L	probably benign	Het
Or5m10	T	A	2: 85,717,482 (GRCm39)	Y113N	probably damaging	Het
Pde4d	T	C	13: 110,087,541 (GRCm39)	I636T	probably benign	Het
Pex5l	C	T	3: 33,008,625 (GRCm39)	V426I	probably damaging	Het
Pmfbp1	A	G	8: 110,252,006 (GRCm39)	K384E	possibly damaging	Het
Pramel22	A	T	4: 143,380,675 (GRCm39)	Y449*	probably null	Het
Prl6a1	T	G	13: 27,498,221 (GRCm39)		probably null	Het
Prss23	A	T	7: 89,159,038 (GRCm39)	W344R	probably damaging	Het
Ptar1	T	A	19: 23,697,680 (GRCm39)	D397E	probably benign	Het
Qrsl1	G	A	10: 43,752,513 (GRCm39)	R437C	probably damaging	Het
Rab6a	G	T	7: 100,257,404 (GRCm39)		probably benign	Het
Samd14	C	A	11: 94,912,239 (GRCm39)	S205R	probably benign	Het
Sel1l3	T	C	5: 53,343,326 (GRCm39)	Y322C	probably damaging	Het
Slc30a5	A	T	13: 100,940,480 (GRCm39)	L669I	probably benign	Het
Ssc4d	A	G	5: 135,991,887 (GRCm39)	L419P	probably damaging	Het
Tbc1d2b	A	T	9: 90,101,063 (GRCm39)	Y642*	probably null	Het
Tbc1d2b	A	C	9: 90,108,301 (GRCm39)	F417V	probably benign	Het
Top2a	T	C	11: 98,907,005 (GRCm39)	D212G	probably damaging	Het
Trim39	A	T	17: 36,571,807 (GRCm39)	V317E	probably damaging	Het
Uncx	G	A	5: 139,530,261 (GRCm39)	R113H	probably damaging	Het
Usp4	A	G	9: 108,256,543 (GRCm39)	S655G	probably benign	Het
Vmn2r28	T	A	7: 5,491,200 (GRCm39)	N349I	probably benign	Het
Vmn2r3	A	G	3: 64,182,770 (GRCm39)	W310R	possibly damaging	Het
Xirp2	T	C	2: 67,340,245 (GRCm39)	W829R	probably damaging	Het
Zfp574	C	A	7: 24,780,777 (GRCm39)	H600N	possibly damaging	Het

Other mutations in Vmn2r114

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Vmn2r114	APN	17	23,510,639 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,510,212 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,509,957 (GRCm39)	missense	probably benign	0.23
IGL00990:Vmn2r114	APN	17	23,509,939 (GRCm39)	missense	probably benign
IGL01838:Vmn2r114	APN	17	23,515,956 (GRCm39)	missense	probably benign	0.44
IGL01990:Vmn2r114	APN	17	23,529,355 (GRCm39)	missense	probably benign	0.22
IGL01994:Vmn2r114	APN	17	23,529,451 (GRCm39)	missense	probably damaging	1.00
IGL02153:Vmn2r114	APN	17	23,510,782 (GRCm39)	missense	probably benign	0.01
IGL02453:Vmn2r114	APN	17	23,530,108 (GRCm39)	missense	probably benign	0.00
IGL02621:Vmn2r114	APN	17	23,529,494 (GRCm39)	missense	probably damaging	0.98
IGL02938:Vmn2r114	APN	17	23,510,263 (GRCm39)	missense	probably benign	0.10
IGL03130:Vmn2r114	APN	17	23,515,970 (GRCm39)	splice site	probably benign
IGL03325:Vmn2r114	APN	17	23,510,652 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r114	UTSW	17	23,510,619 (GRCm39)	missense	probably damaging	1.00
R0109:Vmn2r114	UTSW	17	23,529,549 (GRCm39)	nonsense	probably null
R0164:Vmn2r114	UTSW	17	23,528,800 (GRCm39)	critical splice donor site	probably null
R0310:Vmn2r114	UTSW	17	23,509,917 (GRCm39)	missense	probably benign	0.23
R0583:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R0677:Vmn2r114	UTSW	17	23,529,568 (GRCm39)	missense	probably damaging	1.00
R1127:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1147:Vmn2r114	UTSW	17	23,530,037 (GRCm39)	missense	probably benign	0.00
R1147:Vmn2r114	UTSW	17	23,530,037 (GRCm39)	missense	probably benign	0.00
R1157:Vmn2r114	UTSW	17	23,529,314 (GRCm39)	missense	possibly damaging	0.60
R1323:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1347:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1435:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1437:Vmn2r114	UTSW	17	23,510,185 (GRCm39)	missense	probably damaging	1.00
R1585:Vmn2r114	UTSW	17	23,510,675 (GRCm39)	missense	probably damaging	0.98
R1641:Vmn2r114	UTSW	17	23,515,962 (GRCm39)	missense	probably benign	0.00
R1748:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably benign	0.17
R1954:Vmn2r114	UTSW	17	23,530,086 (GRCm39)	missense	probably benign	0.32
R2081:Vmn2r114	UTSW	17	23,510,083 (GRCm39)	missense	possibly damaging	0.91
R2103:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2113:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2134:Vmn2r114	UTSW	17	23,510,737 (GRCm39)	missense	probably damaging	1.00
R2149:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2424:Vmn2r114	UTSW	17	23,515,842 (GRCm39)	missense	possibly damaging	0.90
R2847:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2848:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2893:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3017:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3018:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3019:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3020:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3021:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4628:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4668:Vmn2r114	UTSW	17	23,529,447 (GRCm39)	missense	possibly damaging	0.83
R4840:Vmn2r114	UTSW	17	23,510,353 (GRCm39)	missense	probably damaging	0.97
R4841:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4842:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4856:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4886:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4992:Vmn2r114	UTSW	17	23,510,765 (GRCm39)	missense	probably benign	0.03
R5182:Vmn2r114	UTSW	17	23,510,632 (GRCm39)	missense	probably damaging	0.96
R5223:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5405:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5449:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5615:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5834:Vmn2r114	UTSW	17	23,529,599 (GRCm39)	missense	possibly damaging	0.90
R6150:Vmn2r114	UTSW	17	23,510,269 (GRCm39)	missense	probably benign	0.03
R6277:Vmn2r114	UTSW	17	23,509,954 (GRCm39)	missense	possibly damaging	0.93
R6403:Vmn2r114	UTSW	17	23,528,939 (GRCm39)	missense	probably damaging	0.99
R6589:Vmn2r114	UTSW	17	23,510,642 (GRCm39)	missense	probably damaging	1.00
R6613:Vmn2r114	UTSW	17	23,529,220 (GRCm39)	missense	possibly damaging	0.82
R6747:Vmn2r114	UTSW	17	23,528,850 (GRCm39)	missense	probably benign	0.00
R6837:Vmn2r114	UTSW	17	23,529,176 (GRCm39)	missense	probably benign	0.10
R6911:Vmn2r114	UTSW	17	23,510,104 (GRCm39)	missense	probably damaging	0.98
R6950:Vmn2r114	UTSW	17	23,529,137 (GRCm39)	missense	probably benign	0.03
R7276:Vmn2r114	UTSW	17	23,509,934 (GRCm39)	missense	probably damaging	0.97
R7482:Vmn2r114	UTSW	17	23,510,468 (GRCm39)	missense	probably damaging	1.00
R7514:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably null	0.96
R7523:Vmn2r114	UTSW	17	23,529,611 (GRCm39)	missense	probably benign	0.01
R7585:Vmn2r114	UTSW	17	23,510,239 (GRCm39)	missense	probably damaging	1.00
R7593:Vmn2r114	UTSW	17	23,510,817 (GRCm39)	nonsense	probably null
R7611:Vmn2r114	UTSW	17	23,515,944 (GRCm39)	missense	probably damaging	0.97
R7641:Vmn2r114	UTSW	17	23,527,177 (GRCm39)	missense	possibly damaging	0.53
R7651:Vmn2r114	UTSW	17	23,509,986 (GRCm39)	nonsense	probably null
R7970:Vmn2r114	UTSW	17	23,530,186 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r114	UTSW	17	23,529,142 (GRCm39)	missense	probably benign	0.36
R8802:Vmn2r114	UTSW	17	23,528,836 (GRCm39)	missense	possibly damaging	0.65
R8847:Vmn2r114	UTSW	17	23,528,986 (GRCm39)	missense	probably damaging	1.00
R8991:Vmn2r114	UTSW	17	23,529,286 (GRCm39)	missense	probably damaging	1.00
R9138:Vmn2r114	UTSW	17	23,510,578 (GRCm39)	missense	probably damaging	1.00
R9173:Vmn2r114	UTSW	17	23,510,527 (GRCm39)	missense	probably damaging	0.99
R9175:Vmn2r114	UTSW	17	23,527,212 (GRCm39)	missense	probably damaging	1.00
R9657:Vmn2r114	UTSW	17	23,510,690 (GRCm39)	missense	probably damaging	1.00
R9670:Vmn2r114	UTSW	17	23,531,098 (GRCm39)	missense
X0065:Vmn2r114	UTSW	17	23,529,931 (GRCm39)	missense	probably benign	0.34
Z1088:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null

Predicted Primers

PCR Primer
(F):5'- GGCAATATTCTTAGTGGAAACAGTG -3'
(R):5'- CCATTGGGAGCTTCACTCTG -3'

Sequencing Primer
(F):5'- CAGTGAGAGTTATTCCAACATCAG -3'
(R):5'- GGGAGCTTCACTCTGGCTTTC -3'

Posted On

2019-10-17