Incidental Mutation 'R8077:Vmn2r66'
| ID |
629077 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Vmn2r66
|
| Ensembl Gene |
ENSMUSG00000094950 |
| Gene Name |
vomeronasal 2, receptor 66 |
| Synonyms |
F830104D24Rik |
| MMRRC Submission |
067511-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.079)
|
| Stock # |
R8077 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
7 |
| Chromosomal Location |
84643853-84661228 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 84656093 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Tyrosine to Asparagine
at position 308
(Y308N)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000122645
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000124773]
|
| AlphaFold |
A0A3B2W842 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000124773
AA Change: Y308N
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000122645
Gene: ENSMUSG00000094950
AA Change: Y308N
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
18 |
N/A |
INTRINSIC |
|
Pfam:ANF_receptor
|
77 |
463 |
5e-31 |
PFAM |
|
Pfam:NCD3G
|
507 |
559 |
6e-21 |
PFAM |
|
Pfam:7tm_3
|
589 |
827 |
3.8e-52 |
PFAM |
|
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.7%
- 10x: 98.8%
- 20x: 95.4%
|
| Validation Efficiency |
100% (60/60) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 61 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1700093K21Rik |
A |
T |
11: 23,467,237 (GRCm39) |
V132E |
probably benign |
Het |
| 4930563M21Rik |
C |
T |
9: 55,895,250 (GRCm39) |
V315M |
probably damaging |
Het |
| Aadacl4fm1 |
T |
C |
4: 144,255,126 (GRCm39) |
I182T |
probably benign |
Het |
| Alb |
G |
C |
5: 90,615,214 (GRCm39) |
R242P |
probably damaging |
Het |
| Amotl1 |
A |
T |
9: 14,461,798 (GRCm39) |
V805D |
probably damaging |
Het |
| Arhgef3 |
T |
C |
14: 27,107,881 (GRCm39) |
L179P |
probably damaging |
Het |
| Atp8b3 |
G |
A |
10: 80,366,858 (GRCm39) |
L247F |
possibly damaging |
Het |
| Ccdc191 |
T |
C |
16: 43,735,968 (GRCm39) |
|
probably null |
Het |
| Celsr3 |
A |
G |
9: 108,705,530 (GRCm39) |
H671R |
probably benign |
Het |
| Cemip |
C |
A |
7: 83,652,616 (GRCm39) |
|
probably benign |
Het |
| Cfap97 |
G |
T |
8: 46,623,482 (GRCm39) |
V291F |
possibly damaging |
Het |
| Clca3a2 |
A |
G |
3: 144,777,288 (GRCm39) |
V861A |
possibly damaging |
Het |
| Cln8 |
A |
T |
8: 14,944,950 (GRCm39) |
D88V |
probably damaging |
Het |
| Col18a1 |
C |
A |
10: 76,916,685 (GRCm39) |
G330V |
unknown |
Het |
| Dis3 |
C |
T |
14: 99,327,471 (GRCm39) |
R344Q |
probably benign |
Het |
| Esyt2 |
T |
A |
12: 116,305,848 (GRCm39) |
S359R |
possibly damaging |
Het |
| Fbxo41 |
A |
T |
6: 85,450,211 (GRCm39) |
L844Q |
probably damaging |
Het |
| Fn1 |
G |
A |
1: 71,651,761 (GRCm39) |
T1372M |
probably damaging |
Het |
| Gbp5 |
G |
A |
3: 142,213,500 (GRCm39) |
R472H |
probably benign |
Het |
| Gm4559 |
C |
T |
7: 141,827,553 (GRCm39) |
R183K |
unknown |
Het |
| Gm9507 |
T |
A |
10: 77,647,604 (GRCm39) |
E25V |
unknown |
Het |
| Golgb1 |
A |
G |
16: 36,738,995 (GRCm39) |
I2486V |
probably damaging |
Het |
| Ifitm10 |
A |
T |
7: 141,924,704 (GRCm39) |
V45D |
probably damaging |
Het |
| Ift80 |
A |
G |
3: 68,823,478 (GRCm39) |
Y595H |
probably benign |
Het |
| Itga8 |
C |
T |
2: 12,247,244 (GRCm39) |
V326I |
probably benign |
Het |
| Kif14 |
A |
T |
1: 136,399,186 (GRCm39) |
H449L |
possibly damaging |
Het |
| Klrh1 |
A |
G |
6: 129,743,658 (GRCm39) |
Y209H |
probably benign |
Het |
| Ldlrad1 |
G |
A |
4: 107,066,688 (GRCm39) |
A8T |
probably benign |
Het |
| Lrrc8b |
C |
A |
5: 105,627,883 (GRCm39) |
S76R |
possibly damaging |
Het |
| Lrrn1 |
T |
C |
6: 107,545,783 (GRCm39) |
L527P |
probably damaging |
Het |
| Luc7l |
T |
C |
17: 26,474,047 (GRCm39) |
V35A |
probably damaging |
Het |
| Luzp1 |
T |
G |
4: 136,270,402 (GRCm39) |
V875G |
probably damaging |
Het |
| Mcf2l |
G |
T |
8: 13,048,494 (GRCm39) |
|
probably null |
Het |
| Mcoln2 |
T |
C |
3: 145,896,169 (GRCm39) |
M497T |
probably damaging |
Het |
| Mrgprb4 |
A |
T |
7: 47,848,203 (GRCm39) |
S242T |
probably benign |
Het |
| Muc21 |
TCAGTGGTGGTCAGGATGGGGGTAGAGCCTGAGCCACTCCTGGATGCAGTGGTGGTCAGG |
TCAGTGGTGGTCAGG |
17: 35,930,628 (GRCm39) |
|
probably benign |
Het |
| Nipbl |
T |
A |
15: 8,340,734 (GRCm39) |
R1995S |
possibly damaging |
Het |
| Nup35 |
A |
G |
2: 80,469,280 (GRCm39) |
|
probably null |
Het |
| Or13a17 |
C |
T |
7: 140,271,046 (GRCm39) |
S76F |
probably benign |
Het |
| Or2o1 |
A |
G |
11: 49,051,312 (GRCm39) |
D157G |
probably damaging |
Het |
| Or6k2 |
A |
G |
1: 173,979,411 (GRCm39) |
|
probably benign |
Het |
| Prdm12 |
A |
G |
2: 31,532,316 (GRCm39) |
K109E |
probably damaging |
Het |
| Ptch1 |
A |
T |
13: 63,688,626 (GRCm39) |
L444Q |
probably damaging |
Het |
| Qtrt1 |
C |
T |
9: 21,331,392 (GRCm39) |
R374* |
probably null |
Het |
| Rnase11 |
T |
C |
14: 51,287,398 (GRCm39) |
D52G |
probably damaging |
Het |
| Rps6 |
A |
G |
4: 86,774,158 (GRCm39) |
S148P |
probably benign |
Het |
| Rrm2b |
T |
C |
15: 37,947,044 (GRCm39) |
K86E |
possibly damaging |
Het |
| Sh2d1b2 |
A |
G |
1: 170,075,742 (GRCm39) |
K59E |
possibly damaging |
Het |
| Six6 |
T |
A |
12: 72,987,100 (GRCm39) |
W91R |
probably damaging |
Het |
| Slc23a2 |
C |
A |
2: 131,931,092 (GRCm39) |
A136S |
possibly damaging |
Het |
| Slc9a5 |
G |
A |
8: 106,086,012 (GRCm39) |
R593H |
probably damaging |
Het |
| Smbd1 |
T |
C |
16: 32,630,804 (GRCm39) |
M1V |
probably null |
Het |
| Ssbp4 |
T |
C |
8: 71,051,647 (GRCm39) |
Y239C |
probably damaging |
Het |
| Stox1 |
T |
C |
10: 62,501,345 (GRCm39) |
E405G |
probably damaging |
Het |
| Tle7 |
C |
T |
8: 110,836,735 (GRCm39) |
T207M |
probably damaging |
Het |
| Tmem192 |
A |
G |
8: 65,418,196 (GRCm39) |
I194V |
probably benign |
Het |
| Tns3 |
A |
T |
11: 8,395,667 (GRCm39) |
C1246S |
probably damaging |
Het |
| Ugt1a6a |
A |
T |
1: 88,066,575 (GRCm39) |
Q127L |
probably benign |
Het |
| Ush2a |
A |
G |
1: 188,275,025 (GRCm39) |
I1833V |
probably benign |
Het |
| Vti1a |
T |
G |
19: 55,564,917 (GRCm39) |
L191R |
probably benign |
Het |
| Zbtb38 |
A |
T |
9: 96,570,153 (GRCm39) |
N310K |
probably benign |
Het |
|
| Other mutations in Vmn2r66 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00321:Vmn2r66
|
APN |
7 |
84,656,299 (GRCm39) |
missense |
probably benign |
|
|
IGL01562:Vmn2r66
|
APN |
7 |
84,656,495 (GRCm39) |
missense |
probably benign |
0.03 |
|
IGL01689:Vmn2r66
|
APN |
7 |
84,657,033 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02237:Vmn2r66
|
APN |
7 |
84,643,908 (GRCm39) |
missense |
probably benign |
|
|
IGL02415:Vmn2r66
|
APN |
7 |
84,656,020 (GRCm39) |
missense |
probably damaging |
0.97 |
|
IGL02439:Vmn2r66
|
APN |
7 |
84,654,455 (GRCm39) |
splice site |
probably benign |
|
|
IGL02545:Vmn2r66
|
APN |
7 |
84,655,798 (GRCm39) |
missense |
possibly damaging |
0.50 |
|
IGL02708:Vmn2r66
|
APN |
7 |
84,655,796 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02794:Vmn2r66
|
APN |
7 |
84,644,623 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02885:Vmn2r66
|
APN |
7 |
84,644,723 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02975:Vmn2r66
|
APN |
7 |
84,656,182 (GRCm39) |
missense |
probably damaging |
0.98 |
|
IGL03027:Vmn2r66
|
APN |
7 |
84,644,777 (GRCm39) |
splice site |
probably benign |
|
|
IGL03081:Vmn2r66
|
APN |
7 |
84,657,138 (GRCm39) |
missense |
probably benign |
|
|
PIT4131001:Vmn2r66
|
UTSW |
7 |
84,644,301 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0098:Vmn2r66
|
UTSW |
7 |
84,654,965 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0504:Vmn2r66
|
UTSW |
7 |
84,656,023 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0557:Vmn2r66
|
UTSW |
7 |
84,643,972 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0617:Vmn2r66
|
UTSW |
7 |
84,644,484 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0883:Vmn2r66
|
UTSW |
7 |
84,657,070 (GRCm39) |
missense |
probably benign |
|
|
R1159:Vmn2r66
|
UTSW |
7 |
84,644,613 (GRCm39) |
missense |
probably benign |
0.44 |
|
R1168:Vmn2r66
|
UTSW |
7 |
84,656,062 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R1172:Vmn2r66
|
UTSW |
7 |
84,654,799 (GRCm39) |
missense |
probably benign |
0.04 |
|
R1175:Vmn2r66
|
UTSW |
7 |
84,654,799 (GRCm39) |
missense |
probably benign |
0.04 |
|
R1538:Vmn2r66
|
UTSW |
7 |
84,644,166 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R1658:Vmn2r66
|
UTSW |
7 |
84,656,955 (GRCm39) |
missense |
probably benign |
0.07 |
|
R1937:Vmn2r66
|
UTSW |
7 |
84,644,344 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1989:Vmn2r66
|
UTSW |
7 |
84,661,201 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2698:Vmn2r66
|
UTSW |
7 |
84,644,607 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2890:Vmn2r66
|
UTSW |
7 |
84,661,027 (GRCm39) |
splice site |
probably null |
|
|
R3686:Vmn2r66
|
UTSW |
7 |
84,644,397 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R4152:Vmn2r66
|
UTSW |
7 |
84,654,800 (GRCm39) |
missense |
probably benign |
0.08 |
|
R4500:Vmn2r66
|
UTSW |
7 |
84,657,162 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4618:Vmn2r66
|
UTSW |
7 |
84,644,296 (GRCm39) |
missense |
possibly damaging |
0.62 |
|
R4656:Vmn2r66
|
UTSW |
7 |
84,661,204 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R4668:Vmn2r66
|
UTSW |
7 |
84,643,905 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4942:Vmn2r66
|
UTSW |
7 |
84,656,980 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5163:Vmn2r66
|
UTSW |
7 |
84,656,017 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5223:Vmn2r66
|
UTSW |
7 |
84,657,093 (GRCm39) |
missense |
probably benign |
|
|
R5377:Vmn2r66
|
UTSW |
7 |
84,656,026 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5512:Vmn2r66
|
UTSW |
7 |
84,657,149 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5611:Vmn2r66
|
UTSW |
7 |
84,654,951 (GRCm39) |
nonsense |
probably null |
|
|
R5749:Vmn2r66
|
UTSW |
7 |
84,655,979 (GRCm39) |
nonsense |
probably null |
|
|
R6131:Vmn2r66
|
UTSW |
7 |
84,644,224 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6183:Vmn2r66
|
UTSW |
7 |
84,644,766 (GRCm39) |
missense |
possibly damaging |
0.81 |
|
R6509:Vmn2r66
|
UTSW |
7 |
84,656,054 (GRCm39) |
missense |
probably benign |
0.12 |
|
R6930:Vmn2r66
|
UTSW |
7 |
84,661,216 (GRCm39) |
missense |
possibly damaging |
0.80 |
|
R6992:Vmn2r66
|
UTSW |
7 |
84,654,436 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R7015:Vmn2r66
|
UTSW |
7 |
84,644,766 (GRCm39) |
missense |
possibly damaging |
0.81 |
|
R7302:Vmn2r66
|
UTSW |
7 |
84,654,423 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7516:Vmn2r66
|
UTSW |
7 |
84,661,176 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
R7763:Vmn2r66
|
UTSW |
7 |
84,654,909 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7814:Vmn2r66
|
UTSW |
7 |
84,656,472 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8307:Vmn2r66
|
UTSW |
7 |
84,656,270 (GRCm39) |
missense |
probably benign |
|
|
R8315:Vmn2r66
|
UTSW |
7 |
84,643,932 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R8490:Vmn2r66
|
UTSW |
7 |
84,654,794 (GRCm39) |
critical splice donor site |
probably null |
|
|
R8511:Vmn2r66
|
UTSW |
7 |
84,656,026 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8781:Vmn2r66
|
UTSW |
7 |
84,644,355 (GRCm39) |
nonsense |
probably null |
|
|
R8812:Vmn2r66
|
UTSW |
7 |
84,654,893 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9203:Vmn2r66
|
UTSW |
7 |
84,654,950 (GRCm39) |
missense |
probably benign |
0.01 |
|
R9277:Vmn2r66
|
UTSW |
7 |
84,661,164 (GRCm39) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- CAGTTGTGGTTGGACAATTCTTCAG -3'
(R):5'- CAAACTGAGAAGATCATGGCCC -3'
Sequencing Primer
(F):5'- CAGCTTCTTACAATTAGATGGTGGC -3'
(R):5'- GGCCCTTAAAGAGTTTCATACGC -3'
|
| Posted On |
2020-06-30 |
Incidental Mutation