Mutagenetix > Incidental Mutation

Incidental Mutation 'R8373:Mettl4'

646558

Institutional Source

Beutler Lab

Gene Symbol

Mettl4

Ensembl Gene

ENSMUSG00000055660

Gene Name

methyltransferase like 4

Synonyms

HsT661, A730091E08Rik, 2410198H06Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.194) question?

Stock #

R8373 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

94727080-94749892 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 94733649 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 359 (T359I)

Ref Sequence

ENSEMBL: ENSMUSP00000127142 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171284]

AlphaFold

Q3U034

Predicted Effect

probably damaging
Transcript: ENSMUST00000171284
AA Change: T359I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000127142
Gene: ENSMUSG00000055660
AA Change: T359I

Domain	Start	End	E-Value	Type
Pfam:MT-A70	280	454	9.9e-43	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210010C04Rik	T	G	6: 41,031,688	T231P	possibly damaging	Het
Adgrg6	T	C	10: 14,467,334	T290A	probably benign	Het
Aff4	C	T	11: 53,400,267	Q685*	probably null	Het
Ankmy1	A	G	1: 92,896,094	M150T	probably damaging	Het
Armc1	T	C	3: 19,149,567	Y65C	probably damaging	Het
Armc8	A	G	9: 99,527,099	V222A	probably benign	Het
Bcar1	A	T	8: 111,715,738	Y228*	probably null	Het
Cct2	T	C	10: 117,060,824	D158G	possibly damaging	Het
Cul1	T	A	6: 47,515,063	C426S	possibly damaging	Het
Deup1	G	T	9: 15,592,375	L297M	possibly damaging	Het
Dpp10	A	G	1: 123,854,229	S74P	possibly damaging	Het
Epha4	A	G	1: 77,507,079	Y98H	possibly damaging	Het
Epn3	T	G	11: 94,492,936	D296A	probably damaging	Het
Eri2	A	C	7: 119,772,597	I252S	probably benign	Het
Gm14025	T	A	2: 129,038,171	I612F		Het
Gp9	C	T	6: 87,779,012	T3I	probably benign	Het
H2-Q5	T	A	17: 35,394,456	V55E		Het
Kif3c	G	T	12: 3,366,089	V37L	probably benign	Het
Lct	A	T	1: 128,303,840	N757K	probably damaging	Het
Lhx8	A	C	3: 154,324,658	N112K	probably damaging	Het
Loxl3	T	A	6: 83,048,891	S373R	possibly damaging	Het
Mpp7	A	G	18: 7,444,096	S109P	probably damaging	Het
Nckap5	A	G	1: 126,026,295	V840A	probably benign	Het
Ncoa4	T	A	14: 32,176,936	L571Q	probably damaging	Het
Olfr453	T	C	6: 42,744,346	F103S	probably damaging	Het
Olfr46	A	T	7: 140,610,295	Y35F	possibly damaging	Het
Phgdh	G	A	3: 98,321,245	T204I	probably damaging	Het
Pla2g4d	C	T	2: 120,277,499	V310M	probably null	Het
Psmd12	C	T	11: 107,497,624	P421L	probably damaging	Het
Ptch1	A	G	13: 63,541,168	Y432H	probably damaging	Het
Rapgef1	G	A	2: 29,710,231	G655S	probably damaging	Het
Rilpl2	C	A	5: 124,478,034	A18S	probably damaging	Het
Srsf7	T	C	17: 80,205,386	R88G	probably benign	Het
St6galnac1	T	C	11: 116,769,233	K85E	possibly damaging	Het
Trip12	A	T	1: 84,795,767	S49R	probably damaging	Het
Wdr48	A	G	9: 119,905,494	T160A	probably damaging	Het
Zfp704	G	A	3: 9,609,442	T93M	unknown	Het

Other mutations in Mettl4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03376:Mettl4	APN	17	94735371	missense	probably damaging	1.00
R1938:Mettl4	UTSW	17	94747857	missense	possibly damaging	0.76
R2172:Mettl4	UTSW	17	94733163	missense	probably benign
R2370:Mettl4	UTSW	17	94733148	missense	probably damaging	1.00
R3621:Mettl4	UTSW	17	94735508	missense	probably damaging	1.00
R3913:Mettl4	UTSW	17	94740532	missense	probably benign	0.06
R4155:Mettl4	UTSW	17	94740575	missense	probably benign
R4536:Mettl4	UTSW	17	94735505	missense	possibly damaging	0.79
R4946:Mettl4	UTSW	17	94740532	missense	probably benign	0.06
R5263:Mettl4	UTSW	17	94740509	nonsense	probably null
R5397:Mettl4	UTSW	17	94727277	nonsense	probably null
R6242:Mettl4	UTSW	17	94735374	missense	probably damaging	1.00
R6508:Mettl4	UTSW	17	94743945	missense	probably damaging	0.98
R7069:Mettl4	UTSW	17	94733633	missense	probably damaging	0.98
R7941:Mettl4	UTSW	17	94733194	splice site	probably null
R8088:Mettl4	UTSW	17	94735367	missense	probably damaging	1.00
R8710:Mettl4	UTSW	17	94733644	missense	probably damaging	0.99
R9055:Mettl4	UTSW	17	94740415	missense	possibly damaging	0.65
R9130:Mettl4	UTSW	17	94735485	missense	possibly damaging	0.61
R9335:Mettl4	UTSW	17	94735508	missense	probably damaging	1.00
R9697:Mettl4	UTSW	17	94727378	missense	probably damaging	0.98
Z1176:Mettl4	UTSW	17	94733563	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CAGCTCTAGGTCACTTTCAGATTTC -3'
(R):5'- ATGCCATGTGAGTGTTCAAAC -3'

Sequencing Primer
(F):5'- TCTAGGTCACTTTCAGATTTCAAAAG -3'
(R):5'- TGCTGGACTTGAAAGACCCACTATG -3'

Posted On

2020-09-02