Mutagenetix > Incidental Mutation

Incidental Mutation 'R9335:Vmn2r60'

707074

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r60

Ensembl Gene

ENSMUSG00000090619

Gene Name

vomeronasal 2, receptor 60

Synonyms

Gprc2a-rs3, Casr-rs3, EG637898

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.117) question?

Stock #

R9335 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

42116471-42195776 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 42194908 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 565 (H565L)

Ref Sequence

ENSEMBL: ENSMUSP00000128493 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166447]

AlphaFold

A0A3B2WBC8

Predicted Effect

probably damaging
Transcript: ENSMUST00000166447
AA Change: H565L

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000128493
Gene: ENSMUSG00000090619
AA Change: H565L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	78	471	1.2e-44	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	1.4e-51	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932438H23Rik	G	T	16: 91,055,942	T102K	probably damaging	Het
Afm	A	T	5: 90,550,227	D496V	probably damaging	Het
Aldh1l2	C	T	10: 83,506,646	M546I	probably damaging	Het
Apol7c	G	T	15: 77,525,689	N352K	probably benign	Het
Arfgef1	A	T	1: 10,158,011	D1319E	probably damaging	Het
Atl2	A	G	17: 79,852,778	I460T	probably benign	Het
Brms1l	C	A	12: 55,841,646	T73K	possibly damaging	Het
C1rl	A	T	6: 124,505,382	H154L	probably benign	Het
Cacna2d4	T	A	6: 119,302,053	L709Q	probably damaging	Het
Cd101	A	T	3: 101,008,115	V716D	probably benign	Het
Cd177	A	G	7: 24,744,286	S796P	probably benign	Het
D430041D05Rik	A	T	2: 104,248,329	M530K	probably damaging	Het
Dcbld2	A	T	16: 58,451,778	Q399L	probably benign	Het
Dennd4b	T	C	3: 90,268,304	Y138H	probably damaging	Het
Dmxl1	A	G	18: 49,859,120	N311S	probably damaging	Het
Dnaic2	A	G	11: 114,734,663	I107V	probably benign	Het
Dync2h1	A	C	9: 7,112,149	V2480G	possibly damaging	Het
Ect2l	T	A	10: 18,201,284	T24S	probably null	Het
Egfr	C	A	11: 16,870,991	T302K	probably damaging	Het
Eid1	T	G	2: 125,673,658	I156S	possibly damaging	Het
Elmod1	A	T	9: 53,935,832	W21R	probably benign	Het
Enah	G	A	1: 181,921,885	P463L	probably damaging	Het
Epha7	A	G	4: 28,966,529	Y602C	probably benign	Het
Ermap	T	A	4: 119,178,348	Y481F	probably damaging	Het
Fam114a2	G	T	11: 57,506,922	T231N	possibly damaging	Het
Gdap1	A	G	1: 17,161,165	T245A	probably benign	Het
Glmp	T	A	3: 88,328,256	V368D	probably damaging	Het
Gm10203	C	T	6: 149,130,317	A40V	unknown	Het
Gm4553	ACCCTTGCAGCCACCACAGGAGCCACAGCCCCCACAGGAGCTACAGCCTCCCTTGCAGCCACCACAGGAGCCACAGCCCCCACAGGAGCTACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCC	ACCCTTGCAGCCACCACAGGAGCCACAGCCCCCACAGGAGCTACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCC	7: 142,165,420		probably benign	Het
Grid1	G	A	14: 35,321,707	D340N	probably damaging	Het
Hao2	C	A	3: 98,883,694	V55L	possibly damaging	Het
Hemgn	A	T	4: 46,394,647	S488T	probably benign	Het
Ighv12-3	C	T	12: 114,366,692	G61E	probably damaging	Het
Itga2b	T	C	11: 102,455,652	E986G	probably damaging	Het
Laptm5	T	G	4: 130,929,528	S167A		Het
Lmtk3	A	T	7: 45,792,741	Y377F	probably damaging	Het
Lrp2	A	G	2: 69,428,639	I4569T	probably benign	Het
Magi2	A	G	5: 20,661,265	N176D		Het
Mcf2l	C	A	8: 13,000,812	H356N	possibly damaging	Het
Mettl4	T	A	17: 94,735,508	Y300F	probably damaging	Het
Naaladl2	T	G	3: 24,413,368	K307N	possibly damaging	Het
Olfr169	G	A	16: 19,566,763	T40I	probably benign	Het
Olfr43	T	A	11: 74,207,006	D70V	probably damaging	Het
Olfr610	C	T	7: 103,506,520	R142H	probably benign	Het
Olfr765	C	T	10: 129,046,745	G106E	probably damaging	Het
Phip	T	A	9: 82,932,926	M248L	probably benign	Het
Picalm	A	G	7: 90,176,283	T300A	probably benign	Het
Ppa1	T	G	10: 61,648,783	V19G	possibly damaging	Het
Ppp4r3a	T	C	12: 101,040,754	Y793C	probably damaging	Het
Prlr	A	T	15: 10,325,271	I177F	probably benign	Het
Prodh	C	A	16: 18,076,296	G457V	probably damaging	Het
Prrt3	T	C	6: 113,498,097	K55E	probably benign	Het
Prx	G	T	7: 27,518,071	V805L	probably damaging	Het
Ptk6	T	G	2: 181,202,353	D51A	probably damaging	Het
Rec114	T	A	9: 58,657,758	Q205L	probably null	Het
Rfwd3	T	G	8: 111,279,935	K510Q	possibly damaging	Het
Sdhb	C	T	4: 140,972,939	R117C	probably benign	Het
Serinc2	T	G	4: 130,264,427	T21P	probably benign	Het
Serpinb3a	A	C	1: 107,047,156	F216V	probably damaging	Het
Sertm1	T	C	3: 54,899,508	D32G	probably damaging	Het
Setx	A	G	2: 29,145,951	D816G	probably benign	Het
Sfxn4	T	A	19: 60,851,056	N188I	probably damaging	Het
Slc26a4	T	C	12: 31,525,554	D711G	probably damaging	Het
Sp9	T	C	2: 73,274,277	F392L	probably damaging	Het
Ssbp1	T	C	6: 40,478,021	L138P	probably damaging	Het
Sv2a	G	A	3: 96,185,272	G96E	probably damaging	Het
Tbc1d9	G	T	8: 83,210,531	G81C	possibly damaging	Het
Tmem67	G	A	4: 12,040,640	Q968*	probably null	Het
Tnni2	T	C	7: 142,444,266	S169P	probably damaging	Het
Tpk1	T	C	6: 43,469,150		probably null	Het
Unc13b	T	A	4: 43,216,123	Y141N	possibly damaging	Het
Unc13b	A	T	4: 43,255,551	E1057D	probably damaging	Het
Usf3	G	T	16: 44,221,573	G2139C	probably damaging	Het
Vmn1r36	A	T	6: 66,716,446	Y148*	probably null	Het
Vmn2r42	A	C	7: 8,194,759	M287R	probably damaging	Het
Zfp518a	G	A	19: 40,912,781	V385I	probably benign	Het
Zfp974	A	T	7: 27,912,051	M83K	probably benign	Het

Other mutations in Vmn2r60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Vmn2r60	APN	7	42136486	missense	probably benign	0.09
IGL01623:Vmn2r60	APN	7	42136486	missense	probably benign	0.09
IGL02363:Vmn2r60	APN	7	42195154	missense	probably benign	0.02
IGL02485:Vmn2r60	APN	7	42195466	missense	possibly damaging	0.54
IGL02651:Vmn2r60	APN	7	42195586	missense	probably damaging	0.99
IGL02660:Vmn2r60	APN	7	42142296	nonsense	probably null
IGL03135:Vmn2r60	APN	7	42136594	missense	probably benign	0.13
IGL03307:Vmn2r60	APN	7	42116547	missense	probably benign	0.14
R0310:Vmn2r60	UTSW	7	42195140	missense	possibly damaging	0.54
R0314:Vmn2r60	UTSW	7	42135561	splice site	probably benign
R0328:Vmn2r60	UTSW	7	42142320	splice site	probably benign
R0464:Vmn2r60	UTSW	7	42135831	missense	probably damaging	0.99
R0755:Vmn2r60	UTSW	7	42195445	missense	probably damaging	1.00
R1119:Vmn2r60	UTSW	7	42194941	missense	possibly damaging	0.68
R1162:Vmn2r60	UTSW	7	42195771	missense	probably benign	0.29
R1241:Vmn2r60	UTSW	7	42137052	missense	probably benign	0.01
R1404:Vmn2r60	UTSW	7	42136787	missense	probably damaging	0.99
R1404:Vmn2r60	UTSW	7	42136787	missense	probably damaging	0.99
R1488:Vmn2r60	UTSW	7	42136713	missense	probably benign	0.17
R1623:Vmn2r60	UTSW	7	42135855	nonsense	probably null
R1628:Vmn2r60	UTSW	7	42136406	nonsense	probably null
R1883:Vmn2r60	UTSW	7	42136670	missense	probably damaging	0.99
R1884:Vmn2r60	UTSW	7	42136670	missense	probably damaging	0.99
R2182:Vmn2r60	UTSW	7	42195507	missense	probably benign	0.06
R2275:Vmn2r60	UTSW	7	42136827	nonsense	probably null
R2847:Vmn2r60	UTSW	7	42136433	missense	probably benign	0.07
R2885:Vmn2r60	UTSW	7	42140979	missense	possibly damaging	0.91
R2894:Vmn2r60	UTSW	7	42135796	missense	probably benign
R2921:Vmn2r60	UTSW	7	42141035	missense	probably damaging	0.98
R2922:Vmn2r60	UTSW	7	42141035	missense	probably damaging	0.98
R3772:Vmn2r60	UTSW	7	42116556	missense	probably benign	0.35
R3820:Vmn2r60	UTSW	7	42135701	missense	probably damaging	0.98
R3822:Vmn2r60	UTSW	7	42135701	missense	probably damaging	0.98
R3872:Vmn2r60	UTSW	7	42136454	missense	probably benign	0.19
R4222:Vmn2r60	UTSW	7	42116528	missense	probably benign	0.08
R4223:Vmn2r60	UTSW	7	42116528	missense	probably benign	0.08
R4224:Vmn2r60	UTSW	7	42116528	missense	probably benign	0.08
R4526:Vmn2r60	UTSW	7	42195243	missense	probably damaging	0.96
R4547:Vmn2r60	UTSW	7	42135663	missense	probably null	0.54
R4840:Vmn2r60	UTSW	7	42135861	missense	probably damaging	1.00
R5173:Vmn2r60	UTSW	7	42195511	missense	probably damaging	0.97
R5231:Vmn2r60	UTSW	7	42137024	missense	possibly damaging	0.93
R5480:Vmn2r60	UTSW	7	42135730	missense	probably damaging	0.98
R5521:Vmn2r60	UTSW	7	42195625	missense	probably damaging	0.99
R5834:Vmn2r60	UTSW	7	42116508	missense	probably benign	0.17
R6038:Vmn2r60	UTSW	7	42194962	missense	probably benign	0.04
R6038:Vmn2r60	UTSW	7	42194962	missense	probably benign	0.04
R6112:Vmn2r60	UTSW	7	42195423	missense	probably damaging	1.00
R6149:Vmn2r60	UTSW	7	42136976	missense	probably damaging	1.00
R6170:Vmn2r60	UTSW	7	42135621	missense	possibly damaging	0.94
R6383:Vmn2r60	UTSW	7	42116471	start codon destroyed	probably null	0.04
R6811:Vmn2r60	UTSW	7	42194886	missense	probably damaging	1.00
R6876:Vmn2r60	UTSW	7	42135663	missense	probably null	0.54
R6997:Vmn2r60	UTSW	7	42142292	missense	probably benign	0.00
R7040:Vmn2r60	UTSW	7	42142242	missense	probably benign	0.00
R7116:Vmn2r60	UTSW	7	42137063	missense	probably benign	0.00
R7128:Vmn2r60	UTSW	7	42195112	missense	probably damaging	0.96
R7232:Vmn2r60	UTSW	7	42136742	missense	possibly damaging	0.83
R7296:Vmn2r60	UTSW	7	42136402	missense	probably benign	0.01
R7376:Vmn2r60	UTSW	7	42195207	missense	probably damaging	1.00
R7526:Vmn2r60	UTSW	7	42195734	frame shift	probably null
R7527:Vmn2r60	UTSW	7	42195734	frame shift	probably null
R7528:Vmn2r60	UTSW	7	42195734	frame shift	probably null
R7764:Vmn2r60	UTSW	7	42195111	missense	probably damaging	0.99
R7843:Vmn2r60	UTSW	7	42195087	missense	probably benign	0.00
R8080:Vmn2r60	UTSW	7	42141097	missense	probably benign	0.30
R8290:Vmn2r60	UTSW	7	42142266	missense	probably damaging	1.00
R8342:Vmn2r60	UTSW	7	42141070	missense	possibly damaging	0.63
R8362:Vmn2r60	UTSW	7	42195530	missense	probably damaging	1.00
R8418:Vmn2r60	UTSW	7	42195426	missense	probably damaging	0.97
R8848:Vmn2r60	UTSW	7	42136745	missense	probably damaging	1.00
R8860:Vmn2r60	UTSW	7	42142230	missense	probably damaging	0.99
R8882:Vmn2r60	UTSW	7	42141094	missense	probably benign	0.00
R8913:Vmn2r60	UTSW	7	42136354	missense	probably benign	0.27
R9190:Vmn2r60	UTSW	7	42195511	missense	probably damaging	0.99
R9229:Vmn2r60	UTSW	7	42142299	missense	possibly damaging	0.95
R9295:Vmn2r60	UTSW	7	42136531	missense	probably benign	0.01
R9796:Vmn2r60	UTSW	7	42135748	missense	probably benign
RF024:Vmn2r60	UTSW	7	42140939	missense	probably benign	0.01
X0023:Vmn2r60	UTSW	7	42141114	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TTGCCACTATAAGCACTGTATAGAC -3'
(R):5'- AGGATGTAGCTGAGAGTCCTG -3'

Sequencing Primer
(F):5'- AGCACTGTATAGACTTGAAATGATTC -3'
(R):5'- CCTGTTATTGGCCTTGACAATG -3'

Posted On

2022-04-18