Mutagenetix > Incidental Mutation

Incidental Mutation 'R9568:Fat4'

721741

Institutional Source

Beutler Lab

Gene Symbol

Fat4

Ensembl Gene

ENSMUSG00000046743

Gene Name

FAT atypical cadherin 4

Synonyms

6030410K14Rik

MMRRC Submission

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9568 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

38886940-39011985 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 38892007 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Serine at position 1683 (I1683S)

Ref Sequence

ENSEMBL: ENSMUSP00000061836 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061260]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000061260
AA Change: I1683S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000061836
Gene: ENSMUSG00000046743
AA Change: I1683S

Domain	Start	End	E-Value	Type
low complexity region	2	21	N/A	INTRINSIC
CA	60	133	4.09e-7	SMART
CA	157	248	4.51e-18	SMART
CA	272	351	7.66e-30	SMART
CA	380	473	2.55e-17	SMART
CA	497	580	8.27e-26	SMART
CA	605	687	6.46e-28	SMART
CA	711	791	1e-24	SMART
CA	815	891	3.78e-20	SMART
CA	915	994	8.6e-24	SMART
CA	1018	1098	7.09e-25	SMART
CA	1122	1208	6.78e-22	SMART
CA	1232	1313	2.63e-28	SMART
CA	1337	1418	7.25e-31	SMART
CA	1442	1527	4.58e-19	SMART
CA	1550	1629	4.52e-9	SMART
CA	1651	1738	1.3e-9	SMART
CA	1762	1839	2.01e-24	SMART
CA	1863	1942	3.11e-21	SMART
CA	1966	2049	5.85e-26	SMART
CA	2072	2152	1.88e-29	SMART
CA	2176	2257	3.06e-29	SMART
CA	2282	2362	2.61e-23	SMART
CA	2386	2466	2.99e-32	SMART
CA	2490	2568	9.92e-6	SMART
CA	2588	2669	6.58e-20	SMART
CA	2692	2773	7.25e-31	SMART
CA	2796	2872	1.69e-22	SMART
CA	2896	2983	3.16e-22	SMART
CA	3007	3089	1.01e-15	SMART
CA	3113	3194	1.25e-25	SMART
CA	3218	3298	7e-15	SMART
CA	3322	3405	3.96e-14	SMART
CA	3428	3510	3.41e-27	SMART
CA	3532	3614	5.64e-19	SMART
EGF	3807	3862	1.78e-2	SMART
EGF_CA	3864	3900	2.36e-16	SMART
EGF_CA	3902	3938	7.99e-14	SMART
EGF	3943	3976	1.24e-1	SMART
LamG	3996	4144	4.08e-19	SMART
EGF	4167	4200	5.88e-3	SMART
LamG	4244	4375	1.76e-23	SMART
EGF	4430	4464	1.41e-5	SMART
low complexity region	4514	4526	N/A	INTRINSIC
low complexity region	4533	4550	N/A	INTRINSIC
low complexity region	4840	4849	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the protocadherin family. This gene may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem Syndrome 2. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2014]
PHENOTYPE: Homozygous inactivation of this gene leads to neonatal lethality, reduced birth body size, curly tails, kyphosis, small lungs, renal cysts, and defects in sternum and vertebrae morphology, neural tube width, cochlear elongation, stereocilia orientation, kidney development, and intestinal elongation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam6a	T	G	12: 113,544,400 (GRCm38)	V131G	possibly damaging	Het
Agr3	G	T	12: 35,948,350 (GRCm38)	M149I	probably benign	Het
Alox12b	T	A	11: 69,164,010 (GRCm38)	C280S	possibly damaging	Het
Amhr2	C	A	15: 102,445,519 (GRCm38)	Q16K	probably benign	Het
Ank1	A	G	8: 23,119,365 (GRCm38)	T1387A	probably benign	Het
B3gnt9	A	C	8: 105,253,571 (GRCm38)	F395C	probably damaging	Het
BC048562	A	G	9: 108,445,706 (GRCm38)	D80G	probably damaging	Het
Ccdc65	T	A	15: 98,722,938 (GRCm38)	M438K	possibly damaging	Het
Ccz1	T	C	5: 144,001,433 (GRCm38)	R240G	probably damaging	Het
Cish	A	T	9: 107,300,394 (GRCm38)	S90C	probably benign	Het
Col5a2	A	T	1: 45,391,838 (GRCm38)	S881T	possibly damaging	Het
Col6a6	G	A	9: 105,780,727 (GRCm38)	T762I	possibly damaging	Het
Cpa1	A	G	6: 30,640,061 (GRCm38)	K49E	probably benign	Het
Cpb1	T	C	3: 20,266,533 (GRCm38)		probably null	Het
Csmd3	A	G	15: 48,287,546 (GRCm38)	S483P	probably damaging	Het
Cul9	G	A	17: 46,520,118 (GRCm38)	T1643I	possibly damaging	Het
Dock2	T	C	11: 34,708,811 (GRCm38)	K314E	possibly damaging	Het
Elp1	G	T	4: 56,786,711 (GRCm38)	P411T	probably damaging	Het
Fam186b	T	G	15: 99,278,690 (GRCm38)	K773T	probably damaging	Het
Fancl	T	A	11: 26,468,672 (GRCm38)	V259E	probably benign	Het
Fcamr	T	A	1: 130,804,619 (GRCm38)	H114Q	probably damaging	Het
Fndc11	T	C	2: 181,222,253 (GRCm38)	F284L	probably damaging	Het
Galns	A	G	8: 122,584,910 (GRCm38)		probably null	Het
Gch1	A	G	14: 47,189,180 (GRCm38)	S30P	probably benign	Het
Gcnt3	A	G	9: 70,035,064 (GRCm38)	I74T	probably benign	Het
Gfral	A	T	9: 76,197,101 (GRCm38)	C210S	probably damaging	Het
Gm26566	G	A	4: 88,722,010 (GRCm38)	A13T	unknown	Het
Gm9936	A	T	5: 114,857,105 (GRCm38)	D150E	unknown	Het
Gsn	C	A	2: 35,283,991 (GRCm38)	D75E	probably benign	Het
Ighd2-7	T	C	12: 113,456,724 (GRCm38)	T5A	possibly damaging	Het
Itsn1	G	T	16: 91,852,894 (GRCm38)	R152L	probably benign	Het
Klhl3	A	T	13: 58,009,312 (GRCm38)	L620Q	probably damaging	Het
Larp7-ps	G	T	4: 92,191,678 (GRCm38)	Y24*	probably null	Het
Lpl	G	A	8: 68,887,583 (GRCm38)	V77M	probably benign	Het
Lrfn3	A	T	7: 30,359,491 (GRCm38)	H436Q	probably benign	Het
Lrp1b	A	G	2: 40,679,215 (GRCm38)	C3965R		Het
Lrrc2	G	A	9: 110,970,160 (GRCm38)		probably null	Het
Manea	C	A	4: 26,340,468 (GRCm38)	D165Y	probably damaging	Het
Myom1	A	T	17: 71,087,481 (GRCm38)	Q992L	probably damaging	Het
Naip5	T	C	13: 100,223,313 (GRCm38)	T472A	probably benign	Het
Naip5	T	A	13: 100,219,830 (GRCm38)	E1092D	probably benign	Het
Nyap1	C	T	5: 137,735,132 (GRCm38)	W546*	probably null	Het
Olfml3	T	G	3: 103,736,966 (GRCm38)	Y129S	possibly damaging	Het
Or4d5	A	T	9: 40,100,568 (GRCm38)	D307E	probably benign	Het
Or5h19	C	A	16: 59,035,850 (GRCm38)	V296F	probably damaging	Het
Or6z5	A	G	7: 6,474,335 (GRCm38)	E75G	probably damaging	Het
Or9k2b	T	A	10: 130,179,945 (GRCm38)	I312F	probably benign	Het
P2rx6	A	C	16: 17,567,436 (GRCm38)		probably null	Het
Plxnb2	A	T	15: 89,160,957 (GRCm38)	Y1095*	probably null	Het
Pmepa1	G	A	2: 173,228,001 (GRCm38)	R216C	probably damaging	Het
Prodh	A	G	16: 18,084,755 (GRCm38)		probably benign	Het
Ptch1	A	G	13: 63,542,173 (GRCm38)	Y389H	probably damaging	Het
Ptgs2	G	T	1: 150,101,091 (GRCm38)		probably null	Het
Rbm14	A	G	19: 4,811,436 (GRCm38)	Y25H	probably damaging	Het
Rfc2	T	C	5: 134,593,258 (GRCm38)	L197P	probably damaging	Het
Rnf19b	G	T	4: 129,073,604 (GRCm38)	W313L	probably damaging	Het
Sh3rf1	G	T	8: 61,372,551 (GRCm38)	A527S	probably benign	Het
Slc12a1	A	G	2: 125,190,298 (GRCm38)	Y623C	probably damaging	Het
Slc25a54	T	A	3: 109,098,616 (GRCm38)	W147R	probably damaging	Het
Slc4a1	A	G	11: 102,356,854 (GRCm38)	L401P	probably damaging	Het
Slc4a7	A	G	14: 14,796,073 (GRCm38)		probably null	Het
Stab2	A	G	10: 86,863,556 (GRCm38)	Y1933H	probably damaging	Het
Supt5	A	T	7: 28,315,263 (GRCm38)	V1037E	probably damaging	Het
Sv2b	A	T	7: 75,125,680 (GRCm38)	H466Q	probably benign	Het
Tmco5	G	A	2: 116,880,249 (GRCm38)	E17K	probably damaging	Het
Tmem69	G	A	4: 116,554,775 (GRCm38)	S12F	probably damaging	Het
Trp53	C	A	11: 69,587,566 (GRCm38)	Y100*	probably null	Het
Trpm7	A	T	2: 126,822,590 (GRCm38)	H956Q	probably benign	Het
Tsnaxip1	G	A	8: 105,842,503 (GRCm38)	R495H	probably benign	Het
Vmn1r237	A	T	17: 21,314,515 (GRCm38)	I167F	probably benign	Het
Vmn1r70	A	G	7: 10,634,365 (GRCm38)	D260G	probably benign	Het
Zbtb38	G	A	9: 96,688,891 (GRCm38)	H47Y	probably damaging	Het
Zfp184	G	T	13: 21,958,727 (GRCm38)	C201F	probably benign	Het
Zfp384	T	A	6: 125,024,833 (GRCm38)	I147N	possibly damaging	Het

Other mutations in Fat4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00235:Fat4	APN	3	38,982,249 (GRCm38)	missense	probably damaging	1.00
IGL00509:Fat4	APN	3	38,889,039 (GRCm38)	missense	probably damaging	1.00
IGL00698:Fat4	APN	3	38,981,145 (GRCm38)	missense	probably benign	0.17
IGL00934:Fat4	APN	3	38,890,673 (GRCm38)	missense	probably damaging	1.00
IGL01063:Fat4	APN	3	38,890,579 (GRCm38)	missense	possibly damaging	0.80
IGL01123:Fat4	APN	3	38,957,269 (GRCm38)	missense	probably benign	0.00
IGL01313:Fat4	APN	3	39,007,201 (GRCm38)	missense	possibly damaging	0.53
IGL01328:Fat4	APN	3	38,980,658 (GRCm38)	missense	probably damaging	1.00
IGL01328:Fat4	APN	3	38,889,991 (GRCm38)	missense	probably damaging	1.00
IGL01374:Fat4	APN	3	38,887,498 (GRCm38)	missense	probably damaging	1.00
IGL01412:Fat4	APN	3	38,891,181 (GRCm38)	missense	probably benign	0.09
IGL01472:Fat4	APN	3	38,888,070 (GRCm38)	missense	probably damaging	1.00
IGL01514:Fat4	APN	3	38,949,534 (GRCm38)	missense	possibly damaging	0.89
IGL01548:Fat4	APN	3	39,009,257 (GRCm38)	missense	probably damaging	1.00
IGL01548:Fat4	APN	3	38,887,758 (GRCm38)	missense	probably damaging	0.99
IGL01576:Fat4	APN	3	38,888,947 (GRCm38)	missense	probably damaging	1.00
IGL01591:Fat4	APN	3	39,010,375 (GRCm38)	nonsense	probably null
IGL01626:Fat4	APN	3	38,951,032 (GRCm38)	missense	probably damaging	1.00
IGL01746:Fat4	APN	3	38,991,731 (GRCm38)	nonsense	probably null
IGL01800:Fat4	APN	3	38,981,729 (GRCm38)	missense	probably damaging	0.99
IGL01815:Fat4	APN	3	38,888,773 (GRCm38)	missense	probably damaging	1.00
IGL01863:Fat4	APN	3	38,970,619 (GRCm38)	splice site	probably benign
IGL01917:Fat4	APN	3	38,889,730 (GRCm38)	missense	possibly damaging	0.89
IGL01936:Fat4	APN	3	38,979,774 (GRCm38)	missense	probably benign	0.10
IGL02060:Fat4	APN	3	39,010,271 (GRCm38)	missense	probably damaging	1.00
IGL02103:Fat4	APN	3	38,889,199 (GRCm38)	missense	probably damaging	0.97
IGL02119:Fat4	APN	3	38,982,939 (GRCm38)	missense	probably benign	0.10
IGL02124:Fat4	APN	3	38,888,404 (GRCm38)	missense	probably damaging	1.00
IGL02164:Fat4	APN	3	38,996,205 (GRCm38)	critical splice donor site	probably null
IGL02182:Fat4	APN	3	38,890,546 (GRCm38)	missense	probably damaging	1.00
IGL02207:Fat4	APN	3	38,951,263 (GRCm38)	missense	probably benign	0.16
IGL02210:Fat4	APN	3	38,891,853 (GRCm38)	missense	probably benign	0.01
IGL02257:Fat4	APN	3	39,001,139 (GRCm38)	missense	probably benign	0.09
IGL02271:Fat4	APN	3	38,979,919 (GRCm38)	missense	probably benign	0.18
IGL02305:Fat4	APN	3	39,009,988 (GRCm38)	missense	probably damaging	1.00
IGL02314:Fat4	APN	3	38,887,630 (GRCm38)	missense	probably damaging	1.00
IGL02455:Fat4	APN	3	38,951,131 (GRCm38)	missense	possibly damaging	0.48
IGL02468:Fat4	APN	3	38,983,046 (GRCm38)	missense	probably benign
IGL02478:Fat4	APN	3	38,888,215 (GRCm38)	missense	probably damaging	1.00
IGL02480:Fat4	APN	3	39,010,430 (GRCm38)	missense	probably damaging	1.00
IGL02487:Fat4	APN	3	38,887,245 (GRCm38)	missense	probably damaging	1.00
IGL02632:Fat4	APN	3	39,002,764 (GRCm38)	missense	probably benign	0.04
IGL02665:Fat4	APN	3	39,002,836 (GRCm38)	missense	probably benign	0.08
IGL02674:Fat4	APN	3	38,983,337 (GRCm38)	missense	probably benign	0.35
IGL02692:Fat4	APN	3	38,951,086 (GRCm38)	missense	probably damaging	1.00
IGL02710:Fat4	APN	3	38,890,595 (GRCm38)	missense	probably damaging	1.00
IGL02803:Fat4	APN	3	38,889,295 (GRCm38)	missense	probably damaging	1.00
IGL02834:Fat4	APN	3	38,956,744 (GRCm38)	missense	probably damaging	1.00
IGL02891:Fat4	APN	3	38,951,273 (GRCm38)	missense	probably damaging	1.00
IGL02982:Fat4	APN	3	38,890,843 (GRCm38)	missense	probably damaging	1.00
IGL02993:Fat4	APN	3	38,957,155 (GRCm38)	missense	probably damaging	1.00
IGL02996:Fat4	APN	3	38,958,525 (GRCm38)	missense	probably damaging	1.00
IGL03029:Fat4	APN	3	38,982,591 (GRCm38)	missense	possibly damaging	0.46
IGL03124:Fat4	APN	3	38,981,552 (GRCm38)	missense	possibly damaging	0.61
IGL03144:Fat4	APN	3	38,956,859 (GRCm38)	missense	possibly damaging	0.68
IGL03149:Fat4	APN	3	38,991,685 (GRCm38)	missense	probably damaging	1.00
IGL03169:Fat4	APN	3	38,957,398 (GRCm38)	missense	probably benign	0.02
IGL03190:Fat4	APN	3	38,981,241 (GRCm38)	missense	probably damaging	1.00
IGL03272:Fat4	APN	3	39,009,703 (GRCm38)	missense	probably benign
IGL03371:Fat4	APN	3	38,983,187 (GRCm38)	missense	possibly damaging	0.65
IGL03372:Fat4	APN	3	38,889,134 (GRCm38)	missense	possibly damaging	0.88
IGL03388:Fat4	APN	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
IGL03394:Fat4	APN	3	38,892,019 (GRCm38)	missense	probably damaging	0.99
IGL03394:Fat4	APN	3	39,009,364 (GRCm38)	missense	probably damaging	1.00
IGL03405:Fat4	APN	3	38,958,450 (GRCm38)	missense	probably benign	0.02
IGL03410:Fat4	APN	3	38,891,176 (GRCm38)	missense	probably damaging	1.00
Asahi	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
Expulsion	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
heineken	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
schlitz	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
PIT4696001:Fat4	UTSW	3	38,982,357 (GRCm38)	missense	probably damaging	0.98
PIT4696001:Fat4	UTSW	3	38,889,004 (GRCm38)	missense	probably benign	0.04
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0078:Fat4	UTSW	3	38,888,931 (GRCm38)	missense	probably benign	0.35
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0201:Fat4	UTSW	3	38,891,596 (GRCm38)	missense	probably damaging	0.99
R0280:Fat4	UTSW	3	38,890,816 (GRCm38)	missense	probably benign
R0357:Fat4	UTSW	3	38,891,227 (GRCm38)	missense	probably damaging	1.00
R0409:Fat4	UTSW	3	38,977,413 (GRCm38)	missense	probably damaging	1.00
R0498:Fat4	UTSW	3	38,980,637 (GRCm38)	missense	probably benign	0.00
R0502:Fat4	UTSW	3	39,002,924 (GRCm38)	missense	probably damaging	0.98
R0506:Fat4	UTSW	3	38,888,314 (GRCm38)	missense	probably benign	0.00
R0532:Fat4	UTSW	3	38,981,721 (GRCm38)	missense	probably benign	0.02
R0616:Fat4	UTSW	3	38,942,870 (GRCm38)	missense	probably damaging	1.00
R0630:Fat4	UTSW	3	39,000,172 (GRCm38)	missense	probably damaging	1.00
R0678:Fat4	UTSW	3	38,889,694 (GRCm38)	missense	probably damaging	1.00
R0685:Fat4	UTSW	3	39,001,178 (GRCm38)	missense	probably benign
R0729:Fat4	UTSW	3	39,000,295 (GRCm38)	splice site	probably benign
R0748:Fat4	UTSW	3	38,887,828 (GRCm38)	missense	possibly damaging	0.67
R0811:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0812:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0830:Fat4	UTSW	3	38,999,109 (GRCm38)	missense	probably benign	0.26
R0841:Fat4	UTSW	3	38,995,998 (GRCm38)	missense	probably damaging	0.99
R0884:Fat4	UTSW	3	38,982,858 (GRCm38)	missense	possibly damaging	0.89
R1056:Fat4	UTSW	3	38,891,392 (GRCm38)	missense	probably damaging	1.00
R1066:Fat4	UTSW	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
R1078:Fat4	UTSW	3	38,983,086 (GRCm38)	missense	probably benign	0.10
R1084:Fat4	UTSW	3	38,979,825 (GRCm38)	missense	possibly damaging	0.88
R1118:Fat4	UTSW	3	38,982,942 (GRCm38)	missense	possibly damaging	0.88
R1213:Fat4	UTSW	3	38,890,371 (GRCm38)	missense	probably benign	0.01
R1418:Fat4	UTSW	3	38,890,813 (GRCm38)	missense	probably damaging	1.00
R1475:Fat4	UTSW	3	38,888,323 (GRCm38)	missense	probably damaging	1.00
R1487:Fat4	UTSW	3	38,995,917 (GRCm38)	missense	possibly damaging	0.77
R1511:Fat4	UTSW	3	38,983,076 (GRCm38)	missense	probably damaging	0.97
R1534:Fat4	UTSW	3	38,890,089 (GRCm38)	missense	probably damaging	1.00
R1558:Fat4	UTSW	3	38,888,986 (GRCm38)	missense	probably damaging	1.00
R1586:Fat4	UTSW	3	38,888,860 (GRCm38)	missense	probably damaging	1.00
R1592:Fat4	UTSW	3	39,007,177 (GRCm38)	missense	probably damaging	0.99
R1655:Fat4	UTSW	3	38,957,318 (GRCm38)	missense	probably damaging	0.97
R1662:Fat4	UTSW	3	38,980,779 (GRCm38)	missense	probably damaging	1.00
R1710:Fat4	UTSW	3	38,951,155 (GRCm38)	missense	probably damaging	1.00
R1731:Fat4	UTSW	3	38,891,310 (GRCm38)	missense	probably damaging	1.00
R1761:Fat4	UTSW	3	38,887,489 (GRCm38)	missense	possibly damaging	0.61
R1770:Fat4	UTSW	3	39,010,268 (GRCm38)	missense	probably damaging	1.00
R1828:Fat4	UTSW	3	38,983,458 (GRCm38)	missense	probably damaging	1.00
R1835:Fat4	UTSW	3	38,983,571 (GRCm38)	missense	probably benign	0.00
R1846:Fat4	UTSW	3	38,982,383 (GRCm38)	missense	probably benign	0.00
R1861:Fat4	UTSW	3	39,010,484 (GRCm38)	missense	probably benign	0.09
R1871:Fat4	UTSW	3	38,981,072 (GRCm38)	missense	possibly damaging	0.63
R1981:Fat4	UTSW	3	38,991,664 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,996,090 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,887,115 (GRCm38)	missense	probably benign
R2056:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2058:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2059:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2070:Fat4	UTSW	3	39,010,655 (GRCm38)	missense	probably benign	0.00
R2078:Fat4	UTSW	3	38,889,673 (GRCm38)	missense	probably damaging	1.00
R2114:Fat4	UTSW	3	38,981,484 (GRCm38)	missense	probably benign	0.01
R2135:Fat4	UTSW	3	38,980,733 (GRCm38)	missense	probably damaging	0.98
R2152:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2153:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2154:Fat4	UTSW	3	38,887,539 (GRCm38)	missense	probably damaging	1.00
R2196:Fat4	UTSW	3	38,981,417 (GRCm38)	missense	probably benign	0.23
R2211:Fat4	UTSW	3	38,891,527 (GRCm38)	missense	possibly damaging	0.77
R2219:Fat4	UTSW	3	39,010,215 (GRCm38)	missense	probably damaging	1.00
R2247:Fat4	UTSW	3	38,892,049 (GRCm38)	missense	probably damaging	1.00
R2263:Fat4	UTSW	3	38,888,989 (GRCm38)	missense	possibly damaging	0.93
R2264:Fat4	UTSW	3	38,890,422 (GRCm38)	missense	probably benign	0.25
R2274:Fat4	UTSW	3	38,995,899 (GRCm38)	missense	possibly damaging	0.47
R2337:Fat4	UTSW	3	38,980,011 (GRCm38)	missense	probably damaging	1.00
R2343:Fat4	UTSW	3	38,957,105 (GRCm38)	missense	probably damaging	0.97
R2365:Fat4	UTSW	3	38,980,419 (GRCm38)	missense	probably benign
R2412:Fat4	UTSW	3	38,957,072 (GRCm38)	missense	probably benign	0.05
R2883:Fat4	UTSW	3	38,980,804 (GRCm38)	missense	probably damaging	1.00
R2942:Fat4	UTSW	3	38,982,336 (GRCm38)	missense	probably damaging	1.00
R2989:Fat4	UTSW	3	39,007,153 (GRCm38)	missense	probably benign
R3103:Fat4	UTSW	3	38,891,940 (GRCm38)	missense	probably benign	0.03
R3158:Fat4	UTSW	3	38,890,791 (GRCm38)	missense	possibly damaging	0.87
R3800:Fat4	UTSW	3	38,981,274 (GRCm38)	missense	possibly damaging	0.48
R3808:Fat4	UTSW	3	38,982,438 (GRCm38)	missense	possibly damaging	0.52
R3848:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3850:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3957:Fat4	UTSW	3	38,982,346 (GRCm38)	missense	probably benign
R4065:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign	0.13
R4078:Fat4	UTSW	3	38,980,020 (GRCm38)	missense	probably damaging	1.00
R4096:Fat4	UTSW	3	38,887,875 (GRCm38)	missense	possibly damaging	0.46
R4161:Fat4	UTSW	3	38,942,809 (GRCm38)	missense	possibly damaging	0.95
R4273:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R4285:Fat4	UTSW	3	38,889,171 (GRCm38)	missense	probably benign	0.00
R4288:Fat4	UTSW	3	38,891,763 (GRCm38)	missense	probably damaging	1.00
R4407:Fat4	UTSW	3	38,958,540 (GRCm38)	missense	probably benign	0.05
R4528:Fat4	UTSW	3	38,891,294 (GRCm38)	missense	probably benign	0.01
R4547:Fat4	UTSW	3	38,951,283 (GRCm38)	missense	probably damaging	1.00
R4681:Fat4	UTSW	3	38,887,342 (GRCm38)	missense	probably damaging	1.00
R4826:Fat4	UTSW	3	38,982,957 (GRCm38)	missense	probably damaging	1.00
R4855:Fat4	UTSW	3	38,888,317 (GRCm38)	missense	probably benign
R4871:Fat4	UTSW	3	38,891,605 (GRCm38)	missense	probably damaging	1.00
R4897:Fat4	UTSW	3	38,980,632 (GRCm38)	missense	probably damaging	1.00
R4928:Fat4	UTSW	3	39,010,465 (GRCm38)	missense	probably damaging	1.00
R4932:Fat4	UTSW	3	39,007,203 (GRCm38)	missense	probably benign	0.00
R4941:Fat4	UTSW	3	38,957,452 (GRCm38)	missense	probably damaging	1.00
R4943:Fat4	UTSW	3	38,980,173 (GRCm38)	missense	probably benign	0.19
R4959:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R4973:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R5098:Fat4	UTSW	3	38,888,289 (GRCm38)	missense	probably benign	0.34
R5163:Fat4	UTSW	3	38,980,797 (GRCm38)	missense	probably damaging	1.00
R5213:Fat4	UTSW	3	38,980,191 (GRCm38)	missense	possibly damaging	0.56
R5328:Fat4	UTSW	3	38,956,868 (GRCm38)	missense	probably damaging	1.00
R5337:Fat4	UTSW	3	39,010,378 (GRCm38)	missense	probably benign	0.44
R5337:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R5363:Fat4	UTSW	3	38,888,005 (GRCm38)	missense	probably damaging	1.00
R5380:Fat4	UTSW	3	38,888,864 (GRCm38)	missense	probably damaging	1.00
R5384:Fat4	UTSW	3	38,995,946 (GRCm38)	missense	possibly damaging	0.87
R5422:Fat4	UTSW	3	38,887,245 (GRCm38)	missense	possibly damaging	0.92
R5436:Fat4	UTSW	3	38,891,346 (GRCm38)	missense	probably benign	0.00
R5443:Fat4	UTSW	3	39,010,370 (GRCm38)	missense	probably damaging	1.00
R5501:Fat4	UTSW	3	38,887,215 (GRCm38)	missense	probably benign	0.09
R5571:Fat4	UTSW	3	39,010,274 (GRCm38)	missense	probably damaging	1.00
R5625:Fat4	UTSW	3	38,888,934 (GRCm38)	missense	possibly damaging	0.78
R5652:Fat4	UTSW	3	39,002,968 (GRCm38)	missense	probably damaging	0.99
R5725:Fat4	UTSW	3	38,889,625 (GRCm38)	missense	probably damaging	1.00
R5735:Fat4	UTSW	3	38,949,576 (GRCm38)	missense	probably damaging	1.00
R5739:Fat4	UTSW	3	38,983,134 (GRCm38)	missense	probably benign	0.01
R5766:Fat4	UTSW	3	38,889,468 (GRCm38)	missense	probably damaging	1.00
R5780:Fat4	UTSW	3	38,980,955 (GRCm38)	missense	probably damaging	0.96
R5811:Fat4	UTSW	3	38,891,787 (GRCm38)	missense	probably damaging	1.00
R5829:Fat4	UTSW	3	39,007,305 (GRCm38)	missense	probably damaging	1.00
R5879:Fat4	UTSW	3	38,887,336 (GRCm38)	missense	probably benign
R5933:Fat4	UTSW	3	38,951,375 (GRCm38)	critical splice donor site	probably null
R5938:Fat4	UTSW	3	38,951,239 (GRCm38)	missense	probably damaging	1.00
R5940:Fat4	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
R5945:Fat4	UTSW	3	38,983,206 (GRCm38)	missense	probably benign	0.19
R5963:Fat4	UTSW	3	39,010,547 (GRCm38)	missense	probably damaging	1.00
R6077:Fat4	UTSW	3	39,002,802 (GRCm38)	missense	probably damaging	1.00
R6158:Fat4	UTSW	3	38,983,262 (GRCm38)	missense	possibly damaging	0.95
R6246:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	1.00
R6253:Fat4	UTSW	3	38,951,356 (GRCm38)	missense	probably damaging	0.99
R6259:Fat4	UTSW	3	39,007,246 (GRCm38)	missense	probably benign	0.18
R6295:Fat4	UTSW	3	39,007,080 (GRCm38)	splice site	probably null
R6387:Fat4	UTSW	3	38,983,785 (GRCm38)	missense	probably damaging	1.00
R6390:Fat4	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
R6456:Fat4	UTSW	3	38,983,979 (GRCm38)	missense	possibly damaging	0.90
R6493:Fat4	UTSW	3	38,890,887 (GRCm38)	missense	probably damaging	1.00
R6500:Fat4	UTSW	3	38,981,269 (GRCm38)	nonsense	probably null
R6503:Fat4	UTSW	3	38,982,257 (GRCm38)	missense	probably benign	0.00
R6519:Fat4	UTSW	3	39,002,871 (GRCm38)	missense	probably benign
R6566:Fat4	UTSW	3	38,957,126 (GRCm38)	missense	possibly damaging	0.78
R6576:Fat4	UTSW	3	38,979,690 (GRCm38)	missense	probably benign
R6590:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6658:Fat4	UTSW	3	38,942,928 (GRCm38)	missense	probably benign	0.01
R6662:Fat4	UTSW	3	38,956,821 (GRCm38)	missense	possibly damaging	0.95
R6690:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6807:Fat4	UTSW	3	38,982,440 (GRCm38)	missense	probably benign	0.18
R6823:Fat4	UTSW	3	38,983,939 (GRCm38)	missense	probably benign	0.05
R6824:Fat4	UTSW	3	38,957,525 (GRCm38)	missense	probably benign	0.00
R6830:Fat4	UTSW	3	38,981,817 (GRCm38)	missense	probably benign	0.00
R6925:Fat4	UTSW	3	38,996,204 (GRCm38)	critical splice donor site	probably null
R6948:Fat4	UTSW	3	39,009,446 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,995,971 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,981,775 (GRCm38)	missense	probably damaging	1.00
R7017:Fat4	UTSW	3	38,891,543 (GRCm38)	missense	probably benign
R7030:Fat4	UTSW	3	38,981,958 (GRCm38)	missense	probably damaging	1.00
R7044:Fat4	UTSW	3	39,010,811 (GRCm38)	missense	probably benign	0.02
R7044:Fat4	UTSW	3	39,010,810 (GRCm38)	missense	probably benign
R7045:Fat4	UTSW	3	38,888,601 (GRCm38)	missense	probably benign	0.01
R7094:Fat4	UTSW	3	38,889,874 (GRCm38)	missense	probably damaging	1.00
R7111:Fat4	UTSW	3	39,010,533 (GRCm38)	missense	probably damaging	1.00
R7130:Fat4	UTSW	3	38,980,787 (GRCm38)	missense	probably damaging	0.99
R7168:Fat4	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
R7192:Fat4	UTSW	3	38,980,464 (GRCm38)	missense	probably benign	0.04
R7194:Fat4	UTSW	3	38,983,895 (GRCm38)	missense	probably damaging	1.00
R7194:Fat4	UTSW	3	38,888,884 (GRCm38)	missense	probably damaging	1.00
R7199:Fat4	UTSW	3	38,977,362 (GRCm38)	missense	probably damaging	0.98
R7213:Fat4	UTSW	3	38,999,087 (GRCm38)	missense	possibly damaging	0.63
R7216:Fat4	UTSW	3	38,891,043 (GRCm38)	missense	probably damaging	1.00
R7225:Fat4	UTSW	3	38,980,176 (GRCm38)	missense	possibly damaging	0.50
R7238:Fat4	UTSW	3	38,890,413 (GRCm38)	missense	probably benign	0.31
R7239:Fat4	UTSW	3	38,983,840 (GRCm38)	missense	possibly damaging	0.85
R7283:Fat4	UTSW	3	38,889,693 (GRCm38)	missense	probably damaging	1.00
R7296:Fat4	UTSW	3	38,889,145 (GRCm38)	nonsense	probably null
R7372:Fat4	UTSW	3	38,890,209 (GRCm38)	missense	probably damaging	1.00
R7400:Fat4	UTSW	3	38,887,924 (GRCm38)	missense	probably damaging	1.00
R7419:Fat4	UTSW	3	39,000,236 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	39,009,644 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	38,887,450 (GRCm38)	missense	probably damaging	0.97
R7431:Fat4	UTSW	3	39,009,157 (GRCm38)	missense	possibly damaging	0.80
R7486:Fat4	UTSW	3	38,957,427 (GRCm38)	nonsense	probably null
R7501:Fat4	UTSW	3	38,958,448 (GRCm38)	nonsense	probably null
R7533:Fat4	UTSW	3	39,007,257 (GRCm38)	missense	probably benign	0.43
R7542:Fat4	UTSW	3	38,981,621 (GRCm38)	missense	possibly damaging	0.64
R7542:Fat4	UTSW	3	38,981,355 (GRCm38)	missense	possibly damaging	0.56
R7548:Fat4	UTSW	3	38,981,114 (GRCm38)	missense	probably benign	0.13
R7567:Fat4	UTSW	3	38,889,336 (GRCm38)	missense	probably damaging	1.00
R7644:Fat4	UTSW	3	39,010,241 (GRCm38)	missense	possibly damaging	0.64
R7660:Fat4	UTSW	3	38,981,160 (GRCm38)	missense	probably benign
R7665:Fat4	UTSW	3	38,889,178 (GRCm38)	missense	probably benign	0.00
R7676:Fat4	UTSW	3	38,891,697 (GRCm38)	missense	probably damaging	0.98
R7832:Fat4	UTSW	3	39,001,204 (GRCm38)	missense	probably benign	0.00
R7848:Fat4	UTSW	3	38,887,851 (GRCm38)	missense	probably benign
R7883:Fat4	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
R7892:Fat4	UTSW	3	38,949,439 (GRCm38)	critical splice acceptor site	probably null
R7904:Fat4	UTSW	3	38,887,541 (GRCm38)	missense	probably damaging	1.00
R7952:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	0.98
R8015:Fat4	UTSW	3	38,981,916 (GRCm38)	missense	possibly damaging	0.79
R8040:Fat4	UTSW	3	38,981,666 (GRCm38)	missense	probably damaging	1.00
R8142:Fat4	UTSW	3	38,891,203 (GRCm38)	missense	probably damaging	1.00
R8151:Fat4	UTSW	3	38,892,054 (GRCm38)	missense	probably damaging	0.99
R8163:Fat4	UTSW	3	38,979,732 (GRCm38)	missense	possibly damaging	0.88
R8317:Fat4	UTSW	3	38,958,510 (GRCm38)	missense	possibly damaging	0.80
R8413:Fat4	UTSW	3	39,008,979 (GRCm38)	critical splice acceptor site	probably null
R8447:Fat4	UTSW	3	38,979,675 (GRCm38)	missense	possibly damaging	0.88
R8458:Fat4	UTSW	3	38,981,553 (GRCm38)	missense	probably benign	0.25
R8509:Fat4	UTSW	3	38,981,903 (GRCm38)	missense	probably benign
R8543:Fat4	UTSW	3	38,977,494 (GRCm38)	missense	probably damaging	1.00
R8679:Fat4	UTSW	3	39,010,693 (GRCm38)	missense	probably damaging	1.00
R8726:Fat4	UTSW	3	39,010,498 (GRCm38)	missense	probably damaging	1.00
R8743:Fat4	UTSW	3	38,888,443 (GRCm38)	missense	probably benign	0.16
R8751:Fat4	UTSW	3	38,891,853 (GRCm38)	missense	probably benign	0.01
R8779:Fat4	UTSW	3	38,979,749 (GRCm38)	missense	probably damaging	1.00
R8797:Fat4	UTSW	3	38,999,129 (GRCm38)	missense	probably benign	0.01
R8860:Fat4	UTSW	3	38,892,120 (GRCm38)	missense	probably benign	0.26
R8955:Fat4	UTSW	3	38,983,629 (GRCm38)	missense	probably benign	0.01
R9053:Fat4	UTSW	3	38,887,175 (GRCm38)	nonsense	probably null
R9071:Fat4	UTSW	3	38,983,449 (GRCm38)	missense	probably benign	0.29
R9088:Fat4	UTSW	3	39,007,299 (GRCm38)	missense	probably benign	0.02
R9100:Fat4	UTSW	3	39,010,654 (GRCm38)	missense
R9180:Fat4	UTSW	3	38,888,407 (GRCm38)	missense	possibly damaging	0.78
R9184:Fat4	UTSW	3	38,982,443 (GRCm38)	missense	probably damaging	0.99
R9201:Fat4	UTSW	3	38,890,930 (GRCm38)	missense	probably damaging	1.00
R9206:Fat4	UTSW	3	39,009,241 (GRCm38)	missense	probably damaging	0.99
R9268:Fat4	UTSW	3	38,888,247 (GRCm38)	missense	probably damaging	1.00
R9278:Fat4	UTSW	3	38,891,022 (GRCm38)	missense	probably benign	0.44
R9287:Fat4	UTSW	3	38,891,632 (GRCm38)	missense	probably damaging	0.98
R9355:Fat4	UTSW	3	38,981,898 (GRCm38)	missense	probably damaging	1.00
R9437:Fat4	UTSW	3	38,891,268 (GRCm38)	missense	probably benign	0.00
R9455:Fat4	UTSW	3	38,891,263 (GRCm38)	missense
R9456:Fat4	UTSW	3	38,888,422 (GRCm38)	missense	possibly damaging	0.50
R9476:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9510:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9511:Fat4	UTSW	3	38,980,653 (GRCm38)	missense	probably damaging	0.98
R9540:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign
R9646:Fat4	UTSW	3	38,981,664 (GRCm38)	missense	probably damaging	1.00
R9683:Fat4	UTSW	3	38,889,183 (GRCm38)	missense	possibly damaging	0.52
R9711:Fat4	UTSW	3	39,001,225 (GRCm38)	missense	probably benign	0.00
X0017:Fat4	UTSW	3	39,009,106 (GRCm38)	missense	probably benign	0.00
X0019:Fat4	UTSW	3	38,981,040 (GRCm38)	missense	probably damaging	1.00
X0020:Fat4	UTSW	3	39,000,151 (GRCm38)	missense	probably damaging	1.00
X0024:Fat4	UTSW	3	38,942,902 (GRCm38)	missense	probably benign	0.43
X0064:Fat4	UTSW	3	38,970,752 (GRCm38)	missense	probably damaging	1.00
Z1088:Fat4	UTSW	3	38,958,492 (GRCm38)	missense	probably benign	0.00
Z1088:Fat4	UTSW	3	38,887,050 (GRCm38)	missense	possibly damaging	0.88
Z1176:Fat4	UTSW	3	38,983,815 (GRCm38)	missense	probably damaging	1.00
Z1176:Fat4	UTSW	3	38,983,359 (GRCm38)	missense	probably benign	0.00
Z1177:Fat4	UTSW	3	38,890,347 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,888,584 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,981,838 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCCTGTTTTCACACAGACCAAG -3'
(R):5'- TGTGACAACCTGCTTCAATTG -3'

Sequencing Primer
(F):5'- GACCAAGTACATAACCATCTTGAAGG -3'
(R):5'- GAGGACTTCTCTATGGCA -3'

Posted On

2022-08-09